Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis.

abstract：:A genotyping study of 285 Hybrid Capture 2 low-risk probe cocktail-positive specimens showed cross-reactivity with several untargeted human papillomavirus genotypes. Cross-reactivity was often clinically beneficial due to the detection of untargeted low-risk genotypes. A total of 8.4% of positive results, usually weak...

journal_title：Journal of clinical microbiology

authors： Poljak M,Kocjan BJ,Kovanda A,Lunar MM,Lepej SZ,Planinic A,Seme K,Vince A

更新日期：2009-08-01 00:00:00

abstract：:We recently reported an outbreak of invasive aspergillosis in the major heart surgery unit of Hospital Gregorio Marañón, Madrid, Spain (T. Peláez, P. Muñoz, J. Guinea, M. Valerio, M. Giannella, C. H. W. Klaassen, and E. Bouza, Clin. Infect. Dis., in press). Aspergillus fumigatus was isolated from clinical samples from...

journal_title：Journal of clinical microbiology

authors： Guinea J,García de Viedma D,Peláez T,Escribano P,Muñoz P,Meis JF,Klaassen CH,Bouza E

更新日期：2011-10-01 00:00:00

abstract：:We compared the new Abbott TestPack (TP) respiratory syncytial virus (RSV) enzyme immunoassay (EIA) with cell culture and two commercial RSV EIAs (from Abbott Diagnostics and Kallestad Laboratories) by using split samples of fresh nasal washings from children with suspected RSV disease. Two tubes of HEp-2 cells were i...

journal_title：Journal of clinical microbiology

authors： Wren CG,Bate BJ,Masters HB,Lauer BA

更新日期：1990-06-01 00:00:00

abstract：:The attenuated bacillus Calmette-Guérin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and other members of the M. tuberculosis complex by conventional methods. Recently, a genomic region designated RD1 was found to be pre...

journal_title：Journal of clinical microbiology

authors： Talbot EA,Williams DL,Frothingham R

更新日期：1997-03-01 00:00:00

abstract：:The pandemic spread of Vibrio parahaemolyticus is an international public health issue. Because of the outbreak potential of the organism, it is critical to establish an internationally recognized molecular subtyping protocol for V. parahaemolyticus that is both rapid and robust as a means to monitor its further sprea...

journal_title：Journal of clinical microbiology

authors： Kam KM,Luey CK,Parsons MB,Cooper KL,Nair GB,Alam M,Islam MA,Cheung DT,Chu YW,Ramamurthy T,Pazhani GP,Bhattacharya SK,Watanabe H,Terajima J,Arakawa E,Ratchtrachenchai OA,Huttayananont S,Ribot EM,Gerner-Smidt P,Swamin

更新日期：2008-08-01 00:00:00

abstract：:A simple enzyme immunoassay has been developed to allow the quantitation of the human response to immunization with pneumococcal polysaccharide. The assay uses the 14-valent vaccine (Pneumovax) as a convenient antigen to adsorb to the solid-phase microdilution plate wells and commercially available isotype-specific an...

journal_title：Journal of clinical microbiology

authors： Shyamala GN,Roberton DM,Hosking CS

更新日期：1988-08-01 00:00:00

abstract：:A solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Rabbit and guinea pig antisera for the assay were raised against purified poliovirus type 3/Fin (strain 3/Fin/K) isolated from a fecal specimen from a meningitis patient during an outbreak of poliomyelitis in Finland in 1984....

journal_title：Journal of clinical microbiology

authors： Ukkonen P,Huovilainen A,Hovi T

更新日期：1986-12-01 00:00:00

abstract：:Coagulase-negative staphylococci obtained from patients with continuous ambulatory peritoneal dialysis-related peritonitis were cultured in nutrient broth in an atmosphere of air containing 5% carbon dioxide (reflecting the CO2 tension found in freshly used dialysate). Significant differences were observed between the...

journal_title：Journal of clinical microbiology

authors： Denyer SP,Davies MC,Evans JA,Finch RG,Smith DG,Wilcox MH,Williams P

更新日期：1990-08-01 00:00:00

abstract：:Viral infections of the central nervous system (CNS) are caused by a variety of viruses, namely, herpesviruses, enteroviruses, and flaviviruses. The similar clinical signs provoked by these viruses make the diagnosis difficult. We report on the simultaneous detection of these major CNS pathogens using amplification by...

journal_title：Journal of clinical microbiology

authors： Korimbocus J,Scaramozzino N,Lacroix B,Crance JM,Garin D,Vernet G

更新日期：2005-08-01 00:00:00

abstract：:The National Committee for Clinical Laboratory Standards has recently changed the oxacillin breakpoint from >/=4 mg/liter to >/=0. 5 mg/liter to detect methicillin-resistant coagulase-negative staphylococci (CoNS) because the previous breakpoint lacked sensitivity. To determine the correlation between the new oxacilli...

journal_title：Journal of clinical microbiology

authors： Hussain Z,Stoakes L,Massey V,Diagre D,Fitzgerald V,El Sayed S,Lannigan R

更新日期：2000-02-01 00:00:00

abstract：:Helcococcus kunzii was isolated by sonication and conventional cultures obtained from a case of infection following total knee prosthesis in an immunocompetent patient. The patient recovered uneventfully. This is the first known case of an H. kunzii prosthetic joint infection. ...

journal_title：Journal of clinical microbiology

authors： Pérez-Jorge C,Cordero J,Marin M,Esteban J

更新日期：2012-02-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay method was used to classify bovine serum as positive, negative, or doubtful for antibodies to Brucella abortus. Spectrophotometric data from assays of 64 serologically positive and 32 serologically negative bovine sera were analyzed statistically to define the range of spectrophoto...

journal_title：Journal of clinical microbiology

authors： Heck FC,Williams JD,Pruett J

更新日期：1980-04-01 00:00:00

abstract：:We compared the BD MAX enteric bacterial panel (EBP) to culture for the detection of Salmonella, Shigella, Campylobacter, and Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157 in seeded stool samples. The EBP panel demonstrated superior sensitivity and reliably detected Salmonella, EHEC O157, Shigel...

journal_title：Journal of clinical microbiology

authors： Anderson NW,Buchan BW,Ledeboer NA

更新日期：2014-04-01 00:00:00

abstract：:A total of 28 unrelated isolates of the Salmonella enterica subsp. enterica serovar dublin (S. dublin) collected during a 6-year period, as well as four samples of the S. dublin live vaccine strain Bovisaloral and its prototype strain S. dublin 442/039, were investigated by different molecular typing methods for the f...

journal_title：Journal of clinical microbiology

authors： Liebisch B,Schwarz S

更新日期：1996-03-01 00:00:00

abstract：:The low incidence of group B streptococcal (GBS) invasive neonatal disease in Mexico has been attributed to the low prevalence of serotype III strains, a major serotype in developed countries. In addition, nontypeable strains account for 12% of the isolates in Mexico and < 1% of the isolates in the United States. In t...

journal_title：Journal of clinical microbiology

authors： Palacios GC,Eskew EK,Solorzano F,Mattingly SJ

更新日期：1997-11-01 00:00:00

abstract：:Mitochondrial cytochrome b (cyt b) genes of 42 strains representing 23 species of the genus Trichosporon were partially sequenced to determine their molecular phylogenetic relationships. Almost half of the 22 strains investigated (from 11 different species) contained introns in their sequences. Analysis of a 396-bp co...

journal_title：Journal of clinical microbiology

authors： Biswas SK,Wang L,Yokoyama K,Nishimura K

更新日期：2005-10-01 00:00:00

abstract：:Three molecular assays were evaluated for the direct detection of Mycobacterium tuberculosis complex bacteria in 125 respiratory and 22 nonrespiratory samples. The overall sensitivities obtained were as follows: GenoType MTBDRplus, 97.9%; GenoType Mycobacteria Direct, 93.7%; Gen-Probe Mycobacterium tuberculosis Amplif...

journal_title：Journal of clinical microbiology

authors： Neonakis IK,Gitti Z,Baritaki S,Petinaki E,Baritaki M,Spandidos DA

更新日期：2009-08-01 00:00:00

abstract：:Visceral and cutaneous leishmaniases are heterogenous entities. The Leishmania species that a given patient harbors usually cannot be determined clinically, and this identification is essential to prescribe the best species-specific therapeutic regimen. Our diagnosis procedure includes a real-time PCR assay targeted a...

journal_title：Journal of clinical microbiology

authors： Foulet F,Botterel F,Buffet P,Morizot G,Rivollet D,Deniau M,Pratlong F,Costa JM,Bretagne S

更新日期：2007-07-01 00:00:00

abstract：:The penicillin-induced L phase of growth of the group B, type III streptococcal strain 76-043 was examined for biochemical properties used for the identification of group B streptococci. After numerous serial subcultures in the cell wall-defective state, this stable L phase continued to produce hemolysin, hippuricase,...

journal_title：Journal of clinical microbiology

authors： Flores AE,Ferrieri P

更新日期：1983-10-01 00:00:00

abstract：:Stool microscopy as performed in clinical parasitology laboratories is a complex procedure with subjective interpretation. Quality assurance (QA) programs often emphasize proficiency testing as an assessment tool. We describe a result reproducibility assessment tool, which can form part of a broader QA program, and wh...

journal_title：Journal of clinical microbiology

authors： Libman MD,Gyorkos TW,Kokoskin E,Maclean JD

更新日期：2008-07-01 00:00:00

abstract：:Plasmids encoding extended-spectrum beta-lactamases of the TEM, SHV, and AmpC families were introduced into common Escherichia coli and Klebsiella pneumoniae hosts to create a homogeneous panel for evaluating the abilities of five test systems to detect resistance to eight beta-lactam antibiotics. Although MICs, as de...

journal_title：Journal of clinical microbiology

authors： Katsanis GP,Spargo J,Ferraro MJ,Sutton L,Jacoby GA

更新日期：1994-03-01 00:00:00

abstract：:A TaqMan PCR was established for identification and quantitation of members of the Ehrlichia phagocytophila group in experimentally infected cows and in Ixodes ricinus ticks. The TaqMan PCR identified a 106-bp section of the 16S rRNA gene by use of a specific fluorogenic probe and two primers. This technique was speci...

journal_title：Journal of clinical microbiology

authors： Pusterla N,Huder JB,Leutenegger CM,Braun U,Madigan JE,Lutz H

更新日期：1999-05-01 00:00:00

abstract：:MICs and minimal bactericidal concentrations (MBCs) were evaluated for the four antibiotics azithromycin, amoxicillin, ceftriaxone, and doxycycline against the three main genospecies of Borrelia burgdorferi sensu lato. In MBC testing, statistically significant differences between the genospecies could be found in 7 ou...

journal_title：Journal of clinical microbiology

authors： Sicklinger M,Wienecke R,Neubert U

更新日期：2003-04-01 00:00:00

abstract：:Infections associated with Neisseria meningitidis are a major public health problem in England, Wales, and Northern Ireland. Currently, over 40% of cases are confirmed directly from clinical specimens using PCR-based methodologies without an organism being isolated. A nested/seminested multilocus sequence typing (MLST...

journal_title：Journal of clinical microbiology

authors： Birtles A,Hardy K,Gray SJ,Handford S,Kaczmarski EB,Edwards-Jones V,Fox AJ

更新日期：2005-12-01 00:00:00

abstract：:The duration of mumps virus RNA detection was studied during a mumps outbreak in a highly vaccinated university population. Seven of the eight reverse transcription-PCR-positive specimens were collected during the first 3 days of parotitis, suggesting that viral shedding is minimal after the first 3 days of symptoms. ...

journal_title：Journal of clinical microbiology

authors： Bitsko RH,Cortese MM,Dayan GH,Rota PA,Lowe L,Iversen SC,Bellini WJ

更新日期：2008-03-01 00:00:00

abstract：:Serological interrelations among 46 strains of Haemophilus somnus of various geographical and pathological origins were studied by agglutination and agglutinin adsorption procedures. Sera prepared against nine representative American and Swiss isolates agglutinated all other strains to various titers. Adsorption of an...

journal_title：Journal of clinical microbiology

authors： Canto GJ,Biberstein EL

更新日期：1982-06-01 00:00:00

abstract：:A methodologic study was performed to compare the polymerase chain reaction (PCR) and Southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (HPV) infection. Three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by c...

journal_title：Journal of clinical microbiology

authors： Schiffman MH,Bauer HM,Lorincz AT,Manos MM,Byrne JC,Glass AG,Cadell DM,Howley PM

更新日期：1991-03-01 00:00:00

abstract：:We have evaluated the diagnostic utility of eleven Toxoplasma gondii recombinant antigens (P22 [SAG2], P24 [GRA1], P25, P28 [GRA2], P29 [GRA7], P30 [SAG1], P35, P41 [GRA4], P54 [ROP2], P66 [ROP1], and P68) in immunoglobulin G (IgG) and IgM recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Following an initi...

journal_title：Journal of clinical microbiology

authors： Aubert D,Maine GT,Villena I,Hunt JC,Howard L,Sheu M,Brojanac S,Chovan LE,Nowlan SF,Pinon JM

更新日期：2000-03-01 00:00:00

abstract：:Swine influenza viruses (SIV) have been shown to sporadically infect humans and are infrequently identified by the Influenza Division of the Centers for Disease Control and Prevention (CDC) after being received as unsubtypeable influenza A virus samples. Real-time reverse transcriptase PCR (rRT-PCR) procedures for det...

journal_title：Journal of clinical microbiology

authors： Shu B,Wu KH,Emery S,Villanueva J,Johnson R,Guthrie E,Berman L,Warnes C,Barnes N,Klimov A,Lindstrom S

更新日期：2011-07-01 00:00:00

abstract：:There are no previous reports of human infection due to Acinetobacter baylyi. In this study, we report on six patients with bacteremia due to A. baylyi, based on analysis of the 16S-23S rRNA intergenic spacer and the 16S rRNA gene. All six patients had multiple underlying diseases. The infection was nosocomially acqui...

journal_title：Journal of clinical microbiology

authors： Chen TL,Siu LK,Lee YT,Chen CP,Huang LY,Wu RC,Cho WL,Fung CP

更新日期：2008-09-01 00:00:00