Evaluation of GenoType mycobacteria direct assay in comparison with Gen-Probe Mycobacterium tuberculosis amplified direct test and GenoType MTBDRplus for direct detection of Mycobacterium tuberculosis complex in clinical samples.

abstract：:A case of pyogenic liver abscess (PLA) due to Rhodococcus equi in an immunocompetent individual was successfully treated by combining surgery and antibiotics. The R. equi-targeted antimicrobial agents erythromycin and rifampin were used only after surgical resection of the lesion and identification of the infective or...

journal_title：Journal of clinical microbiology

authors： Napoleão F,Damasco PV,Camello TC,do Vale MD,de Andrade AF,Hirata R Jr,de Mattos-Guaraldi AL

更新日期：2005-02-01 00:00:00

abstract：:A toxic shock syndrome toxin (TSST) variant with an isoelectric point (pI) of 8.6 produced by an ovine-associated Staphylococcus aureus strain was described previously. Analysis of additional strains associated with sheep, goats, cows, and humans by isoelectric focusing with immunoblotting using monoclonal antibodies ...

journal_title：Journal of clinical microbiology

authors： Ho G,Campbell WH,Bergdoll MS,Carlson E

更新日期：1989-09-01 00:00:00

abstract：:The rise of imported malaria cases and the high fatality rate in Europe make the search for new and easy diagnostic methods necessary. Rapid diagnosis tests (RDTs) are, in part, developed to cover the lack of diagnosis experience. Unfortunately, our data suggest that the accuracy of RDTs is insufficient and could incr...

journal_title：Journal of clinical microbiology

authors： Rubio JM,Buhigas I,Subirats M,Baquero M,Puente S,Benito A

更新日期：2001-07-01 00:00:00

abstract：:We determined the E-Test and National Committee for Clinical Laboratory Standards standardized agar dilution MICs of ceftazidime, ciprofloxacin, piperacillin, and tobramycin for Pseudomonas aeruginosa during tests of 100 rough and mucoid P. aeruginosa isolates from cystic fibrosis patients. The levels of agreement (+/...

journal_title：Journal of clinical microbiology

authors： Marley EF,Mohla C,Campos JM

更新日期：1995-12-01 00:00:00

abstract：:Fosfomycin has been shown to have a wide spectrum of activity against multidrug-resistant Gram-negative bacteria; however, breakpoints have been established only for Escherichia coli or Enterobacterales per the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibilit...

journal_title：Journal of clinical microbiology

authors： Smith EC,Brigman HV,Anderson JC,Emery CL,Bias TE,Bergen PJ,Landersdorfer CB,Hirsch EB

更新日期：2020-09-22 00:00:00

abstract：:The complete nucleotide sequence of one of the immediate-early genes of human herpesvirus 6 variant B was determined and compared with that of variant A reported by Martin et al. (M.D. Martin, J. Nicholas, B. J. Thomson, C. Newman, and R. W. Honess, J. Virol. 65:5381-5390, 1991). While it was reported that two open re...

journal_title：Journal of clinical microbiology

authors： Yamamoto T,Mukai T,Kondo K,Yamanishi K

更新日期：1994-02-01 00:00:00

abstract：:Nosocomial oxacillin-resistant Staphylococcus aureus (ORSA) bloodstream isolates were tested to determine the prevalence of vancomycin heteroresistance. We screened 619 ORSA nosocomial bloodstream isolates from 36 hospitals between 1997 and 2000. Only one isolate exhibiting heterotypic resistance was detected. Thus, v...

journal_title：Journal of clinical microbiology

authors： Tallent SM,Bischoff T,Climo M,Ostrowsky B,Wenzel RP,Edmond MB

更新日期：2002-06-01 00:00:00

abstract：:We have developed a rapid and sensitive assay for the detection of Salmonella serovars in veterinary clinical specimens. This method utilizes a short cultivation period followed by PCR. For detection of the amplified product, an enzyme-linked immunosorbent assay (ELISA)-based oligonucleotide ligation assay (OLA) was u...

journal_title：Journal of clinical microbiology

authors： Stone GG,Oberst RD,Hays MP,McVey S,Chengappa MM

更新日期：1995-11-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed to study the subclass distribution of immunoglobulin G (IgG) specific to the core glycolipid (CGL) of the Re mutant of Salmonella minnesota R595 in serum samples from individuals with an IgG response toward these antigens. In a group of healthy blood donors, we detect...

journal_title：Journal of clinical microbiology

authors： Nys M,Joassin L,Somzee A,Demonty J

更新日期：1988-05-01 00:00:00

abstract：:At the Islet Isolation Laboratory of the Scottish National Blood Transfusion Service, manual sterility testing data show that contamination rates are 57.7% for pancreas transport fluid, 4.3% for postpurification islet samples, and 0% for pretransplant islet samples. This pilot study presents the BacT/Alert System as a...

journal_title：Journal of clinical microbiology

authors： Murray L,McGowan N,Fleming J,Bailey L

更新日期：2014-10-01 00:00:00

abstract：:Monoclonal antibodies (MAb) were produced against the specific Bordetella pertussis antigen pertussis toxin (PT). In preliminary studies, one MAb (IB12) was selected and used in an enzyme-linked dot blot immunoassay to evaluate the ability of the method to detect known amounts of PT in control experiments and to test ...

journal_title：Journal of clinical microbiology

authors： Friedman RL,Paulaitis S,McMillan JW

更新日期：1989-11-01 00:00:00

abstract：:The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tåstrup, Denmark) and the Staph ID 32 API system (API System, BioMérieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated....

journal_title：Journal of clinical microbiology

authors： Renneberg J,Rieneck K,Gutschik E

更新日期：1995-05-01 00:00:00

abstract：:A qualitative multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was developed. Furthermore, a separate assay for the recognition of hepatitis A virus was established to complement the test pattern so that all ...

journal_title：Journal of clinical microbiology

authors： Jokela P,Joki-Korpela P,Maaronen M,Glumoff V,Hyypiä T

更新日期：2005-03-01 00:00:00

abstract：:Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a ...

journal_title：Journal of clinical microbiology

authors： Röder B,Frühwirth K,Vogl C,Wagner M,Rossmanith P

更新日期：2010-11-01 00:00:00

abstract：:A colony immunoblot method was developed for serotyping the capsular polysaccharides expressed by Staphylococcus aureus isolates. The method was rapid and specific and was performed with either polyclonal or monoclonal antibodies specific for each of the capsule types. S. aureus isolates were obtained from patients wi...

journal_title：Journal of clinical microbiology

authors： Lee JC,Liu MJ,Parsonnet J,Arbeit RD

更新日期：1990-12-01 00:00:00

abstract：:A set of universal degenerate primers which amplified, by PCR, a 600-bp oligomer encoding a portion of the 60-kDa heat shock protein (HSP60) of both Staphylococcus aureus and Staphylococcus epidermidis were developed. However, when used as a DNA probe, the 600-bp PCR product generated from S. epidermidis failed to cro...

journal_title：Journal of clinical microbiology

authors： Goh SH,Potter S,Wood JO,Hemmingsen SM,Reynolds RP,Chow AW

更新日期：1996-04-01 00:00:00

abstract：:For up to 20 to 40% of patients chronically infected with hepatitis C virus (HCV), the mode of transmission is still unknown. We demonstrate that tear fluid contains HCV RNA-carrying material with the properties of infectious virus and conclude that smear infection with tear fluid may play a role in HCV transmission. ...

journal_title：Journal of clinical microbiology

authors： Feucht HH,Zöllner B,Schröter M,Altrogge H,Laufs R

更新日期：1995-08-01 00:00:00

abstract：:Campylobacter species are the leading agents of bacterial gastroenteritis in developed countries. In this study 320 specimens of feces from patients with symptoms of acute gastroenteritis were cultured for Campylobacter species by direct plating on modified charcoal cefoperazone deoxycholate agar and by enrichment in ...

journal_title：Journal of clinical microbiology

authors： Maher M,Finnegan C,Collins E,Ward B,Carroll C,Cormican M

更新日期：2003-07-01 00:00:00

abstract：:The first outbreak of Candida haemulonii fungemia is described. The seven isolates from the blood of four neonates were identified by DNA sequencing of the ribosomal DNA. They were all resistant to amphotericin B, fluconazole, and itraconazole. This report highlights the emergence of C. haemulonii as an opportunistic ...

journal_title：Journal of clinical microbiology

authors： Khan ZU,Al-Sweih NA,Ahmad S,Al-Kazemi N,Khan S,Joseph L,Chandy R

更新日期：2007-06-01 00:00:00

abstract：:A total of 322 single and paired serum samples from children (newborn to 10 years old), young adults (18 to 20 years old), pregnant women and their cord serum samples, and elderly people (more than 70 years old) was tested for antibodies to enteric adenovirus types 40 and 41 by neutralization test. Serum samples were ...

journal_title：Journal of clinical microbiology

authors： Shinozaki T,Araki K,Ushijima H,Fujii R

更新日期：1987-09-01 00:00:00

abstract：:Colonization of the gastrointestinal and genitourinary tracts of pregnant women with group B Streptococcus (GBS) can result in vertical transmission to neonates during labor/delivery. GBS infections in neonates can cause severe complications, such as sepsis, meningitis, and pneumonia. Accurate detection is critical be...

journal_title：Journal of clinical microbiology

authors： Shin JH,Pride DT

更新日期：2019-05-24 00:00:00

abstract：:We report five new IS711 chromosomal locations that are specific for marine mammal Brucella groups of strains and useful for their identification and classification. Our data support their current classification into two species, Brucella ceti and B. pinnipedialis, with subgroups in each, but also the possibility of a...

journal_title：Journal of clinical microbiology

authors： Zygmunt MS,Maquart M,Bernardet N,Doublet B,Cloeckaert A

更新日期：2010-10-01 00:00:00

abstract：:The aim of the present study was to evaluate the Helicobacter pylori ClariRes assay (Ingenetix, Vienna, Austria) for the detection of H. pylori infection and the simultaneous clarithromycin susceptibility testing of the H. pylori isolates in stool samples from 100 symptomatic children. The results obtained by this nov...

journal_title：Journal of clinical microbiology

authors： Lottspeich C,Schwarzer A,Panthel K,Koletzko S,Rüssmann H

更新日期：2007-06-01 00:00:00

abstract：:Fifteen common serotypes representative of group C(1) and C(2)Salmonella were characterized using a single set of phages. ...

journal_title：Journal of clinical microbiology

authors： Gershman M

更新日期：1976-02-01 00:00:00

abstract：:We determined the entire nucleotide sequence of phiSa2958-carrying Panton-Valentine leukocidin (PVL) gene, which was lysogenized in a sequence type 5 staphylococcal cassette chromosome mec (SCCmec) type II strain of methicillin-resistant Staphylococcus aureus (MRSA). Based on the nucleotide sequences of PVL phages, we...

journal_title：Journal of clinical microbiology

authors： Ma XX,Ito T,Kondo Y,Cho M,Yoshizawa Y,Kaneko J,Katai A,Higashiide M,Li S,Hiramatsu K

更新日期：2008-10-01 00:00:00

abstract：:The fluorescent amplified-fragment length polymorphism (FAFLP) assay potentially amplifies a unique set of genome fragments from each bacterial clone. It uses stringently hybridizing primers which carry a fluorescent label. Precise fragment sizing is achieved by the inclusion of an internal size standard in every lane...

journal_title：Journal of clinical microbiology

authors： Arnold C,Metherell L,Willshaw G,Maggs A,Stanley J

更新日期：1999-05-01 00:00:00

abstract：:Seven rotavirus strains dually reactive to serotype G1- and G2-specific monoclonal antibodies (MAbs) from hospitalized children with rotavirus diarrhea were culture adapted. Six strains were neutralized with G1 antiserum to a higher titer than that of G2, and one was neutralized with G1 and G2 antisera to equal titers...

journal_title：Journal of clinical microbiology

authors： Ranshing SS,Kelkar SD

更新日期：2003-11-01 00:00:00

abstract：:Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct gram-negative bacteria. IEM should be a valuable tool for confirming presumptive isolates of F. tularensis and may potent...

journal_title：Journal of clinical microbiology

authors： Geisbert TW,Jahrling PB,Ezzell JW Jr

更新日期：1993-07-01 00:00:00

abstract：:Herpes infections are among the most common sexually transmitted diseases and are the most common cause of genital ulcer disease in the United States. This study addresses the changing distribution of herpes simplex virus type 1 (HSV-1) and HSV-2 in patients presenting for evaluation of herpetic infections. Viral cult...

journal_title：Journal of clinical microbiology

authors： Ribes JA,Steele AD,Seabolt JP,Baker DJ

更新日期：2001-09-01 00:00:00

abstract：:We evaluated the performance of three commercially available monoclonal antibodies for confirmation of the presence of Chlamydia pneumoniae in cell culture by examining their abilities to stain inclusions of eight strains of C. pneumoniae. The antibodies tested were two unconjugated C. pneumoniae-specific monoclonal r...

journal_title：Journal of clinical microbiology

authors： Montalban GS,Roblin PM,Hammerschlag MR

更新日期：1994-05-01 00:00:00