Variation of DNA sequence in immediate-early gene of human herpesvirus 6 and variant identification by PCR.

abstract：:High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on ty...

journal_title：Journal of clinical microbiology

authors： Castellanos E,Aranaz A,De Buck J

更新日期：2010-04-01 00:00:00

abstract：:Real-time PCR (rt-PCR) is an important diagnostic tool for the identification of Bordetella pertussis, Bordetella holmesii, and Bordetella parapertussis. Most U.S. public health laboratories (USPHLs) target IS481, present in 218 to 238 copies in the B. pertussis genome and 32 to 65 copies in B. holmesii. The CDC devel...

journal_title：Journal of clinical microbiology

authors： Williams MM,Taylor TH Jr,Warshauer DM,Martin MD,Valley AM,Tondella ML

更新日期：2015-01-01 00:00:00

abstract：:A PCR method for the unequivocal assignment of Haemophilus influenzae capsular type (types a to f) was developed. PCR primers were designed from capsule type-specific DNA sequences cloned from the capsular gene cluster of each of the six capsular types. PCR product was amplified only from the capsular type for which t...

journal_title：Journal of clinical microbiology

authors： Falla TJ,Crook DW,Brophy LN,Maskell D,Kroll JS,Moxon ER

更新日期：1994-10-01 00:00:00

abstract：:This case-control study detected and characterized Shigella and diarrheagenic Escherichia coli (DEC) types among Vietnamese children less than 5 years old. In 249 children with diarrhea and 124 controls, Shigella spp. was an important cause of diarrhea (P < 0.05). We used multiplex PCR and DNA probes to detect enteroi...

journal_title：Journal of clinical microbiology

authors： Hien BT,Scheutz F,Cam PD,Serichantalergs O,Huong TT,Thu TM,Dalsgaard A

更新日期：2008-03-01 00:00:00

abstract：:We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially...

journal_title：Journal of clinical microbiology

authors： McClernon DR,Vavro C,St Clair M

更新日期：2006-06-01 00:00:00

abstract：:Although rotavirus genotype P[6] is one of the three most common VP4 specificities associated with human infection, the relatively few sequence data available in public databases suggest that the genetic variability within P[6] might be presently unexplored. Thus far, two human P[6] lineages (M37-like and AU19-like) a...

journal_title：Journal of clinical microbiology

authors： Bányai K,Martella V,Jakab F,Melegh B,Szücs G

更新日期：2004-09-01 00:00:00

abstract：:Phoenix 100 and Vitek 2 (operating with the current colorimetric cards) are commonly used in hospital laboratories for rapid identification of microorganisms. The present meta-analysis aims to evaluate and compare their performance on Gram-positive and Gram-negative bacteria. The MEDLINE database was searched up to Oc...

journal_title：Journal of clinical microbiology

authors： Chatzigeorgiou KS,Sergentanis TN,Tsiodras S,Hamodrakas SJ,Bagos PG

更新日期：2011-09-01 00:00:00

abstract：:Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of Brucella from a New Zealand patient that appears most closely related to str...

journal_title：Journal of clinical microbiology

authors： McDonald WL,Jamaludin R,Mackereth G,Hansen M,Humphrey S,Short P,Taylor T,Swingler J,Dawson CE,Whatmore AM,Stubberfield E,Perrett LL,Simmons G

更新日期：2006-12-01 00:00:00

abstract：:To halt the human immunodeficiency virus type 1 (HIV-1) epidemic requires interventions that can prevent transmission of numerous HIV-1 subtypes. The most frequently transmitted viruses belong to the subtypes A, B, and C and the circulating recombinant forms (CRFs) AE and AG. A fast one-tube assay that identifies and ...

journal_title：Journal of clinical microbiology

authors： de Baar MP,Timmermans EC,Bakker M,de Rooij E,van Gemen B,Goudsmit J

更新日期：2001-05-01 00:00:00

abstract：:The arylsulfatase isozymes of Mycobacterium fortuitum, M. peregrinum, M. chelonei subsp. chelonei, and M. chelonei subsp. abscessus were examined to determine the isozymal and immunological relationship among the members of the M. fortuitum complex. Cell extracts were subjected to electrophoresis on agarose and polyac...

journal_title：Journal of clinical microbiology

authors： Binko BP,Bartholomew WR

更新日期：1978-08-01 00:00:00

abstract：:Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensit...

journal_title：Journal of clinical microbiology

authors： Levine M,Beck I,Styrchak S,Pepper G,Frenkel L

更新日期：2016-07-01 00:00:00

abstract：:Diagnosis of invasive aspergillosis (IA) requires increasingly rapid molecular methods that enable sensitive detection and discrimination between species. We designed and evaluated a real-time PCR-based method that combined melting temperature (T(m)) calling analysis of a specific probe with high-resolution melting an...

journal_title：Journal of clinical microbiology

authors： Alonso M,Escribano P,Guinea J,Recio S,Simon A,Peláez T,Bouza E,García de Viedma D

更新日期：2012-10-01 00:00:00

abstract：:Vibrio cholerae O139 synonym Bengal recently caused large epidemics of cholera-like disease in Bangladesh and India. We compared the restriction fragment length polymorphisms of ctxA and rRNA genes (ribotypes) in 27 isolates of V. cholerae O139 from patients in Bangladesh and India with those of 48 isolates of V. chol...

journal_title：Journal of clinical microbiology

authors： Faruque SM,Abdul Alim AR,Roy SK,Khan F,Nair GB,Sack RB,Albert MJ

更新日期：1994-04-01 00:00:00

abstract：:Over a 6-year period, 24 extended-spectrum beta-lactamase (ESBL)-producing isolates of Pseudomonas aeruginosa were collected from 18 patients living in a nursing home. These isolates had a delayed development of a red pigment and exhibited a similar antibiotype (resistance to all beta-lactams except for imipenem and t...

journal_title：Journal of clinical microbiology

authors： Dubois V,Arpin C,Noury P,Andre C,Coulange L,Quentin C

更新日期：2005-08-01 00:00:00

abstract：:Current nucleic acid amplification methods to detect Mycobacterium tuberculosis are complex, labor-intensive, and technically challenging. We developed and performed the first analysis of the Cepheid Gene Xpert System's MTB/RIF assay, an integrated hands-free sputum-processing and real-time PCR system with rapid on-de...

journal_title：Journal of clinical microbiology

authors： Helb D,Jones M,Story E,Boehme C,Wallace E,Ho K,Kop J,Owens MR,Rodgers R,Banada P,Safi H,Blakemore R,Lan NT,Jones-López EC,Levi M,Burday M,Ayakaka I,Mugerwa RD,McMillan B,Winn-Deen E,Christel L,Dailey P,Perkins

更新日期：2010-01-01 00:00:00

abstract：:We tested 606 specimens for cytomegalovirus infection. Fifty-two specimens were positive by conventional culture versus forty-three by pre-cytopathic effect detection. Twenty-nine specimens were positive by both methods. Because of toxicity, specimens should be set up on both conventional and shell vial culture system...

journal_title：Journal of clinical microbiology

authors： Rabella N,Drew WL

更新日期：1990-04-01 00:00:00

abstract：:Despite advances in the standardization of quantitative DNAemia tests and efforts to better understand and characterize the performance of reference materials in different assays, it remains unclear how the commutability performance of reference materials is related to intra- and interassay agreement. Building upon pr...

journal_title：Journal of clinical microbiology

authors： Tang L,Su Y,Gu Z,Caliendo AM,Pounds S,Hayden RT

更新日期：2019-01-02 00:00:00

abstract：:Resistance to linezolid is rare in clinical isolates of Enterococcus faecalis. A strain resistant to this antimicrobial but susceptible to vancomycin was found to cause central venous catheter colonization in a patient who never received linezolid. ...

journal_title：Journal of clinical microbiology

authors： Marra AR,Major Y,Edmond MB

更新日期：2006-05-01 00:00:00

abstract：:We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HMA) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes. ...

journal_title：Journal of clinical microbiology

authors： Ito K,Iida M,Yamazaki M,Moriya K,Moroishi S,Yatsuyanagi J,Kurazono T,Hiruta N,Ratchtrachenchai OA

更新日期：2007-03-01 00:00:00

abstract：:Molecular types of the Cryptococcus neoformans/Cryptococcus gattii species complex that infect dogs and cats differ regionally and with host species. Antifungal drug susceptibility can vary with molecular type, but the susceptibility of Cryptococcus isolates from dogs and cats is largely unknown. Cryptococcus isolates...

journal_title：Journal of clinical microbiology

authors： Singer LM,Meyer W,Firacative C,Thompson GR 3rd,Samitz E,Sykes JE

更新日期：2014-06-01 00:00:00

abstract：:We evaluated a new hepatitis C virus RNA assay based on one-stage PCR followed by liquid hybridization with an oligonucleotide probe and compared it with nested-set PCR. The one-stage and nested-set PCR assays had identical sensitivities in analytical experiments and showed 100% concordance when clinical specimens wer...

journal_title：Journal of clinical microbiology

authors： Gretch DR,Wilson JJ,Carithers RL Jr,dela Rosa C,Han JH,Corey L

更新日期：1993-02-01 00:00:00

abstract：:Two hundred ninety isolates of Escherichia coli were investigated for the production of extended-spectrum beta-lactamases (ESBLs). Fourteen (4.8%) of the 290 strains were found to produce ESBLs. Each of the 14 strains produced one or two ESBLs, as follows: 10 strains produced TEM-52, 1 strain produced SHV-2a, 1 strain...

journal_title：Journal of clinical microbiology

authors： Pai H,Lyu S,Lee JH,Kim J,Kwon Y,Kim JW,Choe KW

更新日期：1999-06-01 00:00:00

abstract：:High-risk human papillomavirus (hrHPV) testing is now being introduced as a potential primary screening test for improved detection of cervical precancer and cancer. Current U.S. Food and Drug Administration-approved tests are batch tests that take several hours to complete. A rapid, non-batch test might permit point-...

journal_title：Journal of clinical microbiology

authors： Einstein MH,Smith KM,Davis TE,Schmeler KM,Ferris DG,Savage AH,Gray JE,Stoler MH,Wright TC Jr,Ferenczy A,Castle PE

更新日期：2014-06-01 00:00:00

abstract：:Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The anti...

journal_title：Journal of clinical microbiology

authors： Imbert-Marcille BM,Robillard N,Poirier AS,Coste-Burel M,Cantarovich D,Milpied N,Billaudel S

更新日期：1997-10-01 00:00:00

abstract：:Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures were recovered and examined for LT production. Preservation of the cultures on Dorset Egg Medium at 4 degrees C and pre...

journal_title：Journal of clinical microbiology

authors： Yoh M,Narita I,Honda T,Miwatani T,Nishibuchi M

更新日期：1991-10-01 00:00:00

abstract：:Enterobacteriaceae represent a diverse and medically important family of bacteria that are difficult to identify to the species level using the standard molecular method of 16S rRNA gene sequencing. Prior work has demonstrated the value of dnaJ gene sequence analysis in resolving different members of the family. Howev...

journal_title：Journal of clinical microbiology

authors： McLean K,Rosenthal CA,Sengupta D,Owens J,Cookson BT,Hoffman NG,Salipante SJ

更新日期：2019-10-23 00:00:00

abstract：:An estimated 41,200 people were newly infected with hepatitis C virus (HCV) in 2016 in the United States. Screening tests for antibodies to HCV may generate up to 32% false positivity in low-risk populations. Current Centers for Disease Control and Prevention (CDC) screening recommendations do not require confirmatory...

journal_title：Journal of clinical microbiology

authors： Kodani M,Martin M,de Castro VL,Drobeniuc J,Kamili S

更新日期：2019-02-27 00:00:00

abstract：:Twelve strains of Pseudomonas aeruginosa representing 12 serogroups in the serogrouping schema used in the People's Republic of China were compared with serogroups in the International Antigenic Typing System (IATS). The first eight groups originated in the People's Republic of China, and group II appears to have a ne...

journal_title：Journal of clinical microbiology

authors： Liu PV

更新日期：1987-05-01 00:00:00

abstract：:The object of this study was to develop a test that would measure antibodies directed against group B streptococcal types Ia, Ib, Ic, II, and III. The type-specific carbohydrate antigens were purified, labeled with 125I, and used to develop a radioimmunoassay. This procedure should be particularly useful in testing hu...

journal_title：Journal of clinical microbiology

authors： Wilkinson HW,Jones WL

更新日期：1976-05-01 00:00:00

abstract：:The clinical importance of nondiphtherial Corynebacterium, a ubiquitous member of the normal human microflora of the skin and mucous membrane, for ocular surface infections has been recognized recently. We performed an antimicrobial susceptibility test with Etest strips for three fluoroquinolones (ciprofloxacin, norfl...

journal_title：Journal of clinical microbiology

authors： Eguchi H,Kuwahara T,Miyamoto T,Nakayama-Imaohji H,Ichimura M,Hayashi T,Shiota H

更新日期：2008-02-01 00:00:00