High-level fluoroquinolone resistance in ophthalmic clinical isolates belonging to the species Corynebacterium macginleyi.

abstract：:Nineteen strains of nutritionally variant streptococci (NVS) were tested for bacteriolytic activity and for their production of and sensitivity to streptococcal bacteriocinlike inhibitory substances (BLIS). None appeared to produce BLIS. An inverse relationship was found between the sensitivity to BLIS and the product...

journal_title：Journal of clinical microbiology

authors： Tagg JR,van de Rijn I

更新日期：1991-04-01 00:00:00

abstract：:The development and application of a double-label immunofluorescence method which has the potential to screen for single or dual infections from any site, in single shell vial cultures, is described. In this study, a total of 1,141 ocular specimens were inoculated in shell vials, centrifuged at 15,000 X g for 1 h, inc...

journal_title：Journal of clinical microbiology

authors： Walpita P,Darougar S

更新日期：1989-07-01 00:00:00

abstract：:In summary, it is clear that in vitro susceptibility testing can predict outcome in selected clinical situations. The clearest data are from the fluconazole-treated AIDS patients with oropharyngeal candidiasis. In this setting, the homogeneity of the underlying immune defect, combined with the ease of identification a...

journal_title：Journal of clinical microbiology

authors： Ghannoum MA,Rex JH,Galgiani JN

更新日期：1996-03-01 00:00:00

abstract：:Preemptive ganciclovir (GCV) therapy is adopted increasingly in hematopoietic stem cell transplant (HCT) recipients, but occasional cases of increasing cytomegalovirus (CMV) antigenemia levels occur during preemptive GCV therapy. This prospective study investigated the incidence, risk factors, and clinical outcomes of...

journal_title：Journal of clinical microbiology

authors： Park SY,Lee SO,Choi SH,Kim YS,Woo JH,Sung H,Kim MN,Kim DY,Lee JH,Lee JH,Lee KH,Kim SH

更新日期：2011-12-01 00:00:00

abstract：:Blood culture records from 1994 to 1995 from five U.S. medical centers all using the Difco ESP continuous monitoring blood culture system were reviewed retrospectively. Among a total of 7,362 isolates of bacteria and yeasts, only 0.1% of possibly significant isolates would have been missed had blood cultures been rout...

journal_title：Journal of clinical microbiology

authors： Doern GV,Brueggemann AB,Dunne WM,Jenkins SG,Halstead DC,McLaughlin JC

更新日期：1997-05-01 00:00:00

abstract：:We evaluated a new hepatitis C virus RNA assay based on one-stage PCR followed by liquid hybridization with an oligonucleotide probe and compared it with nested-set PCR. The one-stage and nested-set PCR assays had identical sensitivities in analytical experiments and showed 100% concordance when clinical specimens wer...

journal_title：Journal of clinical microbiology

authors： Gretch DR,Wilson JJ,Carithers RL Jr,dela Rosa C,Han JH,Corey L

更新日期：1993-02-01 00:00:00

abstract：:A total of 563 specimens (234 dermal and 329 genital swabs) from patients suspected of having herpes simplex virus (HSV) infections were processed using two different extraction methods (the MagNA Pure LC system and the swab extraction tube system [SETS]); HSV DNA was amplified by LightCycler PCR. HSV DNA was detected...

journal_title：Journal of clinical microbiology

authors： Issa NC,Espy MJ,Uhl JR,Harmsen WS,Mandrekar JN,Gullerud RE,Davis MD,Smith TF

更新日期：2005-03-01 00:00:00

abstract：:A case of zygomycosis caused by Apophysomyces elegans in a patient having 25% full-thickness burns is described. Amputation of the leg was necessary to control rapid tissue invasion. The fungus was isolated from soil in the burn environment. ...

journal_title：Journal of clinical microbiology

authors： Cooter RD,Lim IS,Ellis DH,Leitch IO

更新日期：1990-09-01 00:00:00

abstract：:We evaluated a commercially available second-generation anti-H. pylori immunoglobulin G enzyme immunoassay (EIA) (Cobas Core Anti-Helicobacter pylori EIA; Roche S. A., Basel, Switzerland) for serodiagnosis of H. pylori infection. The results of the assay were assessed in relation to the results of bacterial culture, u...

journal_title：Journal of clinical microbiology

authors： Goossens H,Glupczynski Y,Burette A,Van den Borre C,Butzler JP

更新日期：1992-01-01 00:00:00

abstract：:Among 23 patients carrying methicillin-resistant Staphylococcus aureus (MRSA) in their anterior nares, 6 (26%) also carried methicillin-susceptible S. aureus (MSSA) as less prevalent flora. In 4 of the 6 patients, the MSSA was unrelated to prevalent MRSA, as determined by pulsed-field gel electrophoresis (PFGE), multi...

journal_title：Journal of clinical microbiology

authors： Boundy S,Zhao Q,Fairbanks C,Folgosa L,Climo M,Archer GL

更新日期：2012-02-01 00:00:00

abstract：:The usefulness of pneumococcal urinary antigen tests (UATs) in severe pneumococcal infection relies heavily on the performance in bacteremic patients. Fluorescence technology and automatic reading of test results may improve UAT performance. We evaluated the automatically read Sofia S. pneumoniae FIA for diagnosing pn...

journal_title：Journal of clinical microbiology

authors： Olofsson E,Özenci V,Athlin S

更新日期：2019-07-26 00:00:00

abstract：:A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. Th...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Tsunomori Y,Seki R

更新日期：2003-11-01 00:00:00

abstract：:We studied the prevalence and species distribution of nontuberculous mycobacteria (NTM) in relation to age in 385 patients with cystic fibrosis (CF) (mean age +/- standard deviation [range], 12.0 +/- 6.1 [1 to 24] years; sex ratio, 0.53) attending three Parisian centers. The overall prevalence of NTM in sputum was 8.1...

journal_title：Journal of clinical microbiology

authors： Pierre-Audigier C,Ferroni A,Sermet-Gaudelus I,Le Bourgeois M,Offredo C,Vu-Thien H,Fauroux B,Mariani P,Munck A,Bingen E,Guillemot D,Quesne G,Vincent V,Berche P,Gaillard JL

更新日期：2005-07-01 00:00:00

abstract：:To define the optimal conditions for human immunodeficiency virus (HIV) detection in microcultures, experiments were conducted with different ratios of patient and donor peripheral blood mononuclear cells (PBMCs). Donor/patient PBMC ratios ranged from 1:1 to 1:125. Optimal results were obtained when 1,500,000 donor ce...

journal_title：Journal of clinical microbiology

authors： Dimitrov DH,Melnick JL,Hollinger FB

更新日期：1990-04-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-acquired and life-threatening infections. Active surveillance programs for MRSA utilize either molecular or culture-based methods. A prospective study was performed to compare the performance of selective and differential chromogenic media...

journal_title：Journal of clinical microbiology

authors： Denys GA,Renzi PB,Koch KM,Wissel CM

更新日期：2013-01-01 00:00:00

abstract：:Shiga toxin (Stx)-producing Escherichia coli (STEC) is the main cause of postdiarrheal hemolytic-uremic syndrome (HUS), a life-threatening clinical complication characterized by hemolytic anemia, thrombocytopenia, and acute renal failure that mainly affects children. A relevant feature of STEC strains is the productio...

journal_title：Journal of clinical microbiology

authors： Melli LJ,Zylberman V,Hiriart Y,Lauche CE,Baschkier A,Pardo R,Miliwebsky E,Chinen I,Rivas M,Goldbaum FA,Ugalde JE,Comerci DJ,Ciocchini AE

更新日期：2020-02-24 00:00:00

abstract：:We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

journal_title：Journal of clinical microbiology

authors： Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

更新日期：1999-03-01 00:00:00

abstract：:Two previously demonstrated sensitive and specific enzyme immunoassays (EIAs) for rotavirus, one using polyclonal and monoclonal antisera (TestPack Rotavirus [TPK]; Abbott Laboratories) and the other using only monoclonal anti-rotavirus antibodies (Rotaclone [RTC]; Cambridge BioScience Corporation), were evaluated as ...

journal_title：Journal of clinical microbiology

authors： Dennehy PH,Gauntlett DR,Spangenberger SE

更新日期：1990-06-01 00:00:00

abstract：:Evolution of the direct repeat region in Mycobacterium tuberculosis has created unique spoligotype signatures specifically associated with IS6110-defined strain families. Spoligotyping signatures may enable the analysis of the strain population structure in different settings and will enable the rapid identification o...

journal_title：Journal of clinical microbiology

authors： Streicher EM,Victor TC,van der Spuy G,Sola C,Rastogi N,van Helden PD,Warren RM

更新日期：2007-01-01 00:00:00

abstract：:We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The d...

journal_title：Journal of clinical microbiology

authors： Korbsrisate S,Suwanasai N,Leelaporn A,Ezaki T,Kawamura Y,Sarasombath S

更新日期：1999-11-01 00:00:00

abstract：:In this study, disk diffusion testing with ceftizoxime and cefuroxime was evaluated for use in predicting the susceptibility of Streptococcus pneumoniae to ceftriaxone and cefotaxime. Of the 194 isolates included in this study, 138 were susceptible, 34 were intermediate, and 22 were resistant to cefotaxime by MIC test...

journal_title：Journal of clinical microbiology

authors： Williams-Bouyer N,Hernandez A,Reisner BS

更新日期：1999-11-01 00:00:00

abstract：:Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Epidemiological studies of GBS infections require comprehensive typing systems that provide informa...

journal_title：Journal of clinical microbiology

authors： Sun Y,Kong F,Zhao Z,Gilbert GL

更新日期：2005-09-01 00:00:00

abstract：:Twenty (1.4%) of 1,421 adult Ixodes pacificus ticks and 2 (20%) of 10 adult Ixodes neotomae ticks collected in five counties of northern California were found to contain spirochetes by direct immunofluorescence examination of their tissues with a polyvalent conjugate. Borreliae isolated from the tissues of nine of the...

journal_title：Journal of clinical microbiology

authors： Lane RS,Pascocello JA

更新日期：1989-10-01 00:00:00

abstract：:To objectively assess the value of examining multiple sputum specimens in maximizing the sensitivity of detection of Mycobacterium tuberculosis, we retrospectively reviewed the acid-fast bacillus smear and culture results of patients diagnosed with culture-proven pulmonary tuberculosis (TB) at Hennepin County Medical ...

journal_title：Journal of clinical microbiology

authors： Nelson SM,Deike MA,Cartwright CP

更新日期：1998-02-01 00:00:00

abstract：:Because of their biofilm-forming capacity, invasive Staphylococcus epidermidis isolates, which cause the majority of nosocomial catheter-related bloodstream infections (BSIs), are thought to be selected at the time of catheter insertion from a population of less virulent commensal strains. This fact allows the predict...

journal_title：Journal of clinical microbiology

authors： Rohde H,Kalitzky M,Kröger N,Scherpe S,Horstkotte MA,Knobloch JK,Zander AR,Mack D

更新日期：2004-12-01 00:00:00

abstract：:Both Staphylococcus epidermidis and Staphylococcus aureus are important causes of infections associated with catheters and other medical devices. It has recently been shown that not only S. epidermidis but also S. aureus can produce slime and carries the ica operon responsible for slime production. In the operon, coex...

journal_title：Journal of clinical microbiology

authors： Arciola CR,Baldassarri L,Montanaro L

更新日期：2001-06-01 00:00:00

abstract：:At various times after orthopedic operations (more than a few weeks, with an average of 29.9 days), 11 patients had a sudden onset of high temperature (average 38.9 degrees C) and local cellulitis at different sites on the operated sides. The wounds had completely healed, without complicated infections, when the cellu...

journal_title：Journal of clinical microbiology

authors： Kitamura T,Kawamura Y,Ohkusu K,Masaki T,Iwashita H,Sawa T,Fujii S,Okamoto T,Akaike T

更新日期：2007-01-01 00:00:00

abstract：:The specific identification of verocytotoxin (VT)-producing Escherichia coli (VTEC) requires the detection of VTs in bacterial culture filtrates or the detection of genes encoding these toxins in bacterial cells by specific DNA probes. The standard method for detecting these toxins involves a time-consuming, labor-int...

journal_title：Journal of clinical microbiology

authors： Basta M,Karmali M,Lingwood C

更新日期：1989-07-01 00:00:00

abstract：:Fifty staphylococcal strains that produced toxic shock syndrome (TSS) toxin 1 and that were isolated from patients with TSS were characterized. One strain had more properties that were characteristic of Staphylococcus hyicus than of Staphylococcus aureus. Forty-four strains had the same properties or differed in only ...

journal_title：Journal of clinical microbiology

authors： Aliu B,Bergdoll MS

更新日期：1988-11-01 00:00:00

abstract：:Sepsis is a major health problem in newborns and children. Early detection of pathogens allows initiation of appropriate antimicrobial therapy that strongly correlates with positive outcomes. Multiplex PCR has the potential to rapidly identify bloodstream infections, compensating for the loss of blood culture sensitiv...

journal_title：Journal of clinical microbiology

authors： Lucignano B,Ranno S,Liesenfeld O,Pizzorno B,Putignani L,Bernaschi P,Menichella D

更新日期：2011-06-01 00:00:00