Development and Evaluation of a Novel VHH-Based Immunocapture Assay for High-Sensitivity Detection of Shiga Toxin Type 2 (Stx2) in Stool Samples.

abstract：:A gag-based molecular beacon assay utilizing real-time nucleic acid sequence-based amplification technology has been developed to differentiate between the two genetic subclusters of human immunodeficiency virus type 1 (HIV-1) subtype C (C and C') circulating in Ethiopia. Of 41 samples, 36 could be classified as C or ...

journal_title：Journal of clinical microbiology

authors： Ayele W,Pollakis G,Abebe A,Fisseha B,Tegbaru B,Tesfaye G,Mengistu Y,Wolday D,van Gemen B,Goudsmit J,Dorigo-Zetsma W,de Baar MP

更新日期：2004-04-01 00:00:00

abstract：:We prospectively evaluated a new PCR-enzyme-linked immunosorbent assay kit (Onychodiag; BioAdvance, France) for the diagnosis of dermatophytic onychomycosis by testing nail samples from 438 patients with suspected onychomycosis and from 108 healthy controls in three independent laboratories. In two laboratories, sampl...

journal_title：Journal of clinical microbiology

authors： Savin C,Huck S,Rolland C,Benderdouche M,Faure O,Noacco G,Menotti J,Candolfi E,Pelloux H,Grillot R,Coupe S,Derouin F

更新日期：2007-04-01 00:00:00

abstract：:Legionella is the causative agent for Legionnaires' disease (LD) and is responsible for several large outbreaks in the world. More than 90% of LD cases are caused by Legionella pneumophila, and studies on the origin and transmission routes of this pathogen rely on adequate molecular characterization of isolates. Curre...

journal_title：Journal of clinical microbiology

authors： Bosch T,Euser SM,Landman F,Bruin JP,IJzerman EP,den Boer JW,Schouls LM

更新日期：2015-10-01 00:00:00

abstract：:The increasing risk of Rift Valley fever virus (RVFV) infection as a global veterinary and public health threat demands the development of safe and accurate diagnostic tests. The aim of this study was to assess the suitability of a baculovirus expression system to produce recombinant RVFV nucleoprotein (N) for use as ...

journal_title：Journal of clinical microbiology

authors： Faburay B,Wilson WC,Secka A,Drolet B,McVey DS,Richt JA

更新日期：2019-09-24 00:00:00

abstract：:Two closely related species of Rochalimaea, Rochalimaea quintana and Rochalimaea henselae, are nutritionally fastidious but can be cultivated on bacteriologic media from the blood of patients with diverse clinical presentations. We report a case of culture-proven R. henselae bacteremia in a child with persistent fever...

journal_title：Journal of clinical microbiology

authors： Welch DF,Hensel DM,Pickett DA,San Joaquin VH,Robinson A,Slater LN

更新日期：1993-09-01 00:00:00

abstract：:A total of 25 isolates of vanB-containing Enterococcus faecium were recovered from patients in a single Korean hospital over a 20-month period. There were two distinct vanB2 patterns among the 11 pulsed-field gel electrophoresis types; 17 contained the prototype vanB2 and 8 contained a novel vanB2 with a 177-bp deleti...

journal_title：Journal of clinical microbiology

authors： Lee WG,Jernigan JA,Rasheed JK,Anderson GJ,Tenover FC

更新日期：2001-03-01 00:00:00

abstract：:Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific prim...

journal_title：Journal of clinical microbiology

authors： Githui WA,Wilson SM,Drobniewski FA

更新日期：1999-04-01 00:00:00

abstract：:We report herein a case of Bartholin's gland abscess caused by Brucella melitensis. Clinical microbiology laboratory workers in areas where this disease is endemic should be familiar with the bacteriological features of this organism and consider the possibility of a brucellar etiology in a broad range of clinical set...

journal_title：Journal of clinical microbiology

authors： Peled N,David Y,Yagupsky P

更新日期：2004-02-01 00:00:00

abstract：:Human adenovirus of strains subgenus F (AdV F) are the most common strains detected in acute gastroenteritis cases in developing countries. Subgenus F is represented by AdV serotype 40 (AdV-40) and AdV-41. Most of the reports have described the predominance of AdV-41 in acute gastroenteritis cases. To gain insight int...

journal_title：Journal of clinical microbiology

authors： Dey RS,Ghosh S,Chawla-Sarkar M,Panchalingam S,Nataro JP,Sur D,Manna B,Ramamurthy T

更新日期：2011-02-01 00:00:00

abstract：:Owing to the difficulties in isolating the virus and the lack of routine surveillance, the clinical significance of human parainfluenza virus 4 (HPIV-4) is less well defined than that of the other human parainfluenza viruses. We describe the first outbreak of HPIV-4 infection in a developmental disabilities unit, invo...

journal_title：Journal of clinical microbiology

authors： Lau SK,To WK,Tse PW,Chan AK,Woo PC,Tsoi HW,Leung AF,Li KS,Chan PK,Lim WW,Yung RW,Chan KH,Yuen KY

更新日期：2005-09-01 00:00:00

abstract：:We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 infl...

journal_title：Journal of clinical microbiology

authors： Xing Z,Cardona C,Dao P,Crossley B,Hietala S,Boyce W

更新日期：2008-05-01 00:00:00

abstract：:We report on a reverse line blot (RLB) assay, utilizing fungal species-specific oligonucleotide probes to hybridize with internal transcribed spacer 2 region sequences amplified using a nested panfungal PCR. Reference and clinical strains of 16 Candida species (116 strains), Cryptococcus neoformans (five strains of Cr...

journal_title：Journal of clinical microbiology

authors： Playford EG,Kong F,Sun Y,Wang H,Halliday C,Sorrell TC

更新日期：2006-03-01 00:00:00

abstract：:We report Campylobacter fetus meningitis associated with endocarditis in a 75-year-old diabetic man after he consumed raw liver. C. fetus was isolated from blood samples and cerebrospinal fluid. Cure was obtained with combined intravenous imipenem-gentamicin for 4 weeks; no relapse occurred after 6 months of follow-up...

journal_title：Journal of clinical microbiology

authors： Suy F,Le Dû D,Roux AL,Hanachi M,Dinh A,Crémieux AC

更新日期：2013-09-01 00:00:00

abstract：:The clinical, histological, and bacteriological findings in five cases of acute appendicitis caused by Haemophilus segnis are reported. This is the first documentation of appendicitis associated with this organism. ...

journal_title：Journal of clinical microbiology

authors： Welch WD,Southern PM Jr,Schneider NR

更新日期：1986-11-01 00:00:00

abstract：:Early diagnosis of Neisseria gonorrhoeae infections is important with regard to patients' health and infectivity. We report the development of a specific and sensitive TaqMan assay for the detection of N. gonorrhoeae in clinical samples. The target sequence is a 76-bp fragment of the 5' untranslated region of the opa ...

journal_title：Journal of clinical microbiology

authors： Geraats-Peters CW,Brouwers M,Schneeberger PM,van der Zanden AG,Bruisten SM,Weers-Pothoff G,Boel CH,van den Brule AJ,Harmsen HG,Hermans MH

更新日期：2005-11-01 00:00:00

abstract：:Bacterial vaginosis (BV) remains the most common cause of abnormal vaginal discharge in women of reproductive age and is associated with increased susceptibility to human immunodeficiency virus and sexually transmitted infections and preterm delivery. Present diagnostic methods require access to microscopy and laborat...

journal_title：Journal of clinical microbiology

authors： Bradshaw CS,Morton AN,Garland SM,Horvath LB,Kuzevska I,Fairley CK

更新日期：2005-03-01 00:00:00

abstract：:Sepsis is a major health problem in newborns and children. Early detection of pathogens allows initiation of appropriate antimicrobial therapy that strongly correlates with positive outcomes. Multiplex PCR has the potential to rapidly identify bloodstream infections, compensating for the loss of blood culture sensitiv...

journal_title：Journal of clinical microbiology

authors： Lucignano B,Ranno S,Liesenfeld O,Pizzorno B,Putignani L,Bernaschi P,Menichella D

更新日期：2011-06-01 00:00:00

abstract：:In comparative studies, Hemo-De (PMP Medical Industries, Inc., Irving, Tex.) was found to be a suitable replacement for ethyl acetate in the Formalin-ethyl acetate concentration technique. With essentially equivalent recovery rates for both procedures, the Formalin-Hemo-De concentration technique is considered to be t...

journal_title：Journal of clinical microbiology

authors： Neimeister R,Logan AL,Gerber B,Egleton JH,Kleger B

更新日期：1987-02-01 00:00:00

abstract：:In October 2001, the first disseminated biological warfare attack was perpetrated on American soil. Initially, a few clinical microbiology laboratories were testing specimens from acutely ill patients and also being asked to test nasal swabs from the potentially exposed. Soon after, a significant number of clinical mi...

journal_title：Journal of clinical microbiology

authors： Craft DW,Lee PA,Rowlinson MC

更新日期：2014-07-01 00:00:00

abstract：:A rapid dot blot hybridization assay for the detection of B19 parvovirus DNA in human sera was developed. Small portions of four serum samples were mixed, filtered onto a nylon membrane, and hybridized with a digoxigenin-labeled DNA probe; for each membrane, 380 serum samples could be tested. When a dot was positive b...

journal_title：Journal of clinical microbiology

authors： Zerbini M,Musiani M,Venturoli S,Gallinella G,Gibellini D,Gentilomi G,La Placa M

更新日期：1990-11-01 00:00:00

abstract：:Sequence detection by the 5' nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp. The test was evaluated with 73 lung isolat...

journal_title：Journal of clinical microbiology

authors： Angen O,Jensen J,Lavritsen DT

更新日期：2001-01-01 00:00:00

abstract：:A microhematocrit concentration method (MH) for immediate diagnosis of Chagas' disease during the acute stage or in congenital cases was standardized. Parasitemia as low as 1,000 parasites per ml was detected, after centrifugation of six 50-microliters capillary tubes, by 10-min microscopic observation of each buffy c...

journal_title：Journal of clinical microbiology

authors： Feilij H,Muller L,Gonzalez Cappa SM

更新日期：1983-08-01 00:00:00

abstract：:Characteristics differentiating Escherichia coli strains that cause cystitis or pyelonephritis from fecal E. coli remain incompletely defined, particularly among adult women in the United States. Accordingly, phylogenetic group, O antigens, and virulence factors (VFs) were analyzed among 329 E. coli isolates from the ...

journal_title：Journal of clinical microbiology

authors： Johnson JR,Owens K,Gajewski A,Kuskowski MA

更新日期：2005-12-01 00:00:00

abstract：:Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussi...

journal_title：Journal of clinical microbiology

authors： Vielemeyer O,Crouch JY,Edberg SC,Howe JG

更新日期：2004-02-01 00:00:00

abstract：:Streptococcus agalactiae causes severe invasive disease in humans and mastitis in cattle. Temporally matched bovine milk isolates and clinical human invasive isolates (52 each) collected in New York State over 18 months were characterized by molecular subtyping and phenotypic methods to probe the interspecies transmis...

journal_title：Journal of clinical microbiology

authors： Sukhnanand S,Dogan B,Ayodele MO,Zadoks RN,Craver MP,Dumas NB,Schukken YH,Boor KJ,Wiedmann M

更新日期：2005-03-01 00:00:00

abstract：:A novel PCR primer pair was used to detect the presence of cmeC in 131 Campylobacter jejuni and C. coli strains isolated from various hosts (cattle, turkeys, humans, and pigs). DNA sequence analysis revealed a high degree of genetic variation between the two species, while extremely limited genetic variation among iso...

journal_title：Journal of clinical microbiology

authors： Fakhr MK,Logue CM

更新日期：2007-10-01 00:00:00

abstract：:The first case of cavitary pulmonary disease caused by Purpureocillium lilacinum is described. The isolate showed atypical microscopic characteristics similar to Acremonium and Fusarium spp., which necessitated molecular identification by sequencing of multiple conserved loci. The patient responded to voriconazole, re...

journal_title：Journal of clinical microbiology

authors： Khan Z,Ahmad S,Al-Ghimlas F,Al-Mutairi S,Joseph L,Chandy R,Sutton DA,Guarro J

更新日期：2012-05-01 00:00:00

abstract：:False sensitivities were occasionally encountered in antibiotic-free chambers of the Autobac I system. This unusual phenomenon occurred randomly among gram-negative bacilli and appears to be colistin-related. ...

journal_title：Journal of clinical microbiology

authors： Duckenfield L,Wadke M,Ashton JK

更新日期：1980-10-01 00:00:00

abstract：:Accreditation in Ontario, Canada, requires that licensed clinical laboratories participate in external quality assessment (also known as proficiency testing) and perform competency evaluation of their staff. To assess the extent of ongoing competency assessment practices, the Quality Management Program--Laboratory Ser...

journal_title：Journal of clinical microbiology

authors： Desjardins M,Fleming CA

更新日期：2014-08-01 00:00:00

abstract：:Haemophilus influenzae type b (Hib) is, in contrast to non-type b H. influenzae, associated with severe invasive disease, such as meningitis and epiglottitis, in small children. To date, accurate H. influenzae capsule typing requires PCR, a time-consuming and cumbersome method. Matrix-assisted laser desorption ionizat...

journal_title：Journal of clinical microbiology

authors： Månsson V,Resman F,Kostrzewa M,Nilson B,Riesbeck K

更新日期：2015-07-01 00:00:00