Abstract:
:A gag-based molecular beacon assay utilizing real-time nucleic acid sequence-based amplification technology has been developed to differentiate between the two genetic subclusters of human immunodeficiency virus type 1 (HIV-1) subtype C (C and C') circulating in Ethiopia. Of 41 samples, 36 could be classified as C or C' by sequencing of the gag gene. All 36 isolates were correctly identified by the gag beacon test. Three isolates with genomes that were recombinant in gag were unambiguously typed as belonging to the C' subcluster. Further analysis revealed that these contained the most sequence homology with a reference subcluster C' sequence in the target region of the beacon and hence were correct for the analyzed region. For one sample, sequencing and gag molecular beacon results did not match, while another isolate could not be detected at all by the beacon assay. Overall, high levels of sensitivity and specificity were achieved for both beacons (90.5% sensitivity and 100% specificity for the C beacon and 100% sensitivity and 95.2% specificity for the C' beacon). The availability of a diagnostic test which can quickly and reliably discriminate between C and C' HIV-1 infections in Ethiopia is an important first step toward studying their respective biological characteristics. As the assay is specific to the Ethiopian HIV-1 subtype C epidemic, it will contribute to characterizing the circulating viruses in this population, thereby generating the information necessary for the development of a potential efficacious HIV-1 vaccine appropriate for the Ethiopian context.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Ayele W,Pollakis G,Abebe A,Fisseha B,Tegbaru B,Tesfaye G,Mengistu Y,Wolday D,van Gemen B,Goudsmit J,Dorigo-Zetsma W,de Baar MPdoi
10.1128/jcm.42.4.1534-1541.2004subject
Has Abstractpub_date
2004-04-01 00:00:00pages
1534-41issue
4eissn
0095-1137issn
1098-660Xjournal_volume
42pub_type
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.16.5.861-864.1982
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.11.2832-2836.1994
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.3.633-641.1992
更新日期:1992-03-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.16.1.53-59.1982
更新日期:1982-07-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.4.1441-1446.2002
更新日期:2002-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.5.1068-1070.1990
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.14.1.6-14.1981
更新日期:1981-07-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.8.2118-2121.1992
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journal_title:Journal of clinical microbiology
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更新日期:2002-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.4.739-742.1988
更新日期:1988-04-01 00:00:00
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pub_type: 杂志文章,多中心研究
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journal_title:Journal of clinical microbiology
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更新日期:2011-06-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.8.1720-1724.1990
更新日期:1990-08-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:2020-03-25 00:00:00