Abstract:
:An enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of human immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by using lipopolysaccharides as antigens is described. The results obtained with the lipopolysaccharide ELISA were compared with the results of the whole bacterium ELISA. The correlations observed were good for each immunoglobulin class. Cross-reactions between Y. enterocolitica serotypes O:3 and O:9, Yersinia pseudotuberculosis IA, and Brucella abortus were studied by human and rabbit antisera in the whole bacterium and lipopolysaccharide ELISAs and by rabbit antisera using ELISA inhibition. The greatest cross-reactivity observed was that of the anti-Brucella serum with Y. enterocolitica O:9 in the whole bacterium ELISA. In the lipopolysaccharide ELISA this cross-reaction was not demonstrable with the rabbit antiserum, but it was strong with the human antiserum. However, differential diagnosis was possible with ELISA inhibition. On the basis of our experience, we are now routinely using whole bacterium ELISA for the determination of class-specific Yersinia antibodies, and potential cross-reactions are controlled by the ELISA inhibition.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Granfors K,Viljanen MK,Toivanen Adoi
10.1128/JCM.14.1.6-14.1981subject
Has Abstractpub_date
1981-07-01 00:00:00pages
6-14issue
1eissn
0095-1137issn
1098-660Xjournal_volume
14pub_type
杂志文章abstract::By serological methods, all 83 known types of Streptococcus pneumoniae could be shown to possess C polysaccharide and F antigen. Cross-reactions due to these two antigens between pneumococci and a broad range of most other commonly encountered streptococci were examined. The presence of an antigen closely similar or i...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.10.1854-1859.1987
更新日期:1987-10-01 00:00:00
abstract::A total of 1,835 Yersinia spp. were isolated from 925 (60.5%) of 1,530 wild mice and from 139 (79.9%) of 174 moles living in mountainous areas of eastern Shimane Prefecture, Japan. The Yersinia spp. included 1,106 Yersinia enterocolitica, 26 Y. enterocolitica-like, 176 Yersinia mollaretii, 149 Yersinia frederiksenii, ...
journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.28.11.2448-2455.1990
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pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.4.601-604.1983
更新日期:1983-04-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.7.1742-1745.1995
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.1.199-201.1999
更新日期:1999-01-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.11.5.522-526.1980
更新日期:1980-05-01 00:00:00
abstract::An enzyme-linked immunosorbent assay for antibodies to Borrelia burgdorferi in sheep was established. The assay was used in a seroepidemiological survey in which serum samples from 327 Norwegian rams were screened for antibodies to B. burgdorferi. These rams were randomly chosen, clinically healthy animals from all pa...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.5.1271-1277.1992
更新日期:1992-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.16.5.861-864.1982
更新日期:1982-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章,评审
doi:10.1128/jcm.41.11.5302-5307.2003
更新日期:2003-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.3.1332-1336.2003
更新日期:2003-03-01 00:00:00
abstract::High incidence rates of isoniazid-, rifampin-, and multiple-drug-resistant tuberculosis have been reported in countries of the former Soviet Union (FSU). Genotypic (unlike phenotypic) drug resistance assays do not require viable cultures but require accurate knowledge of both the target gene and the mutations associat...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.10.4498-4502.2004
更新日期:2004-10-01 00:00:00
abstract::A new blood lysis-filtration culture technique revealed a high incidence of Staphylococcus epidermidis in the blood of patients and of healthy people. Of 2,004 blood cultures from patients, the blood lysis method grew S. epidermidis in 233 (11.6%), whereas a conventional two-bottle culture system grew this organism in...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.4.628-630.1983
更新日期:1983-04-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.6.2351-2353.2001
更新日期:2001-06-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.11.4201-4207.2000
更新日期:2000-11-01 00:00:00
abstract::Collecting whole blood on filter paper simplifies the processing, transport, and storage of specimens used for the diagnosis of human immunodeficiency virus type 1 (HIV-1) and other tests. Specimens may be collected in tropical or rural areas with minimal facilities for handling specimens. To compare simulated tropica...
journal_title:Journal of clinical microbiology
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更新日期:2008-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.9.2548-2553.1998
更新日期:1998-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.2.615-6192002
更新日期:2002-02-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.15.5.902-905.1982
更新日期:1982-05-01 00:00:00
abstract::Pulsed-field gel electrophoresis (PFGE) of 61 Salmonella enterica serotype Paratyphi C isolates from six countries gave five distinct clusters. Twenty-four isolates from five countries were susceptible to 10 antimicrobials tested and gave similar restriction endonuclease digest patterns of the 38-MDa plasmid. In contr...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.6.2058-2060.1999
更新日期:1999-06-01 00:00:00
abstract::The polymerase chain reaction (PCR) was investigated for detecting human parvovirus B19 (B19) DNA in sera. Three pairs of oligonucleotides were evaluated as primers. The best oligonucleotide pair spanned 699 nucleotides, including the region common to VP1 and VP2. After PCR amplification of B19 DNA in serum, a 699-nuc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.1.65-69.1990
更新日期:1990-01-01 00:00:00