Detection of human parvovirus B19 DNA by using the polymerase chain reaction.

abstract：:Selective media for Vibrio parahaemolyticus, BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar, were modified for use in immunological detection of the thermostable direct hemolysin produced by this organism. The modified BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar were both...

journal_title：Journal of clinical microbiology

authors： Honda T,Sornchai C,Takeda Y,Miwatani T

更新日期：1982-10-01 00:00:00

abstract：:Candida dubliniensis is often associated with C. albicans in cultures. Easy-to-perform selective isolation procedures for these closely related species do not exist. Therefore, we evaluated previously described discriminatory phenotypic markers for C. dubliniensis. A total of 150 oral rinses from human immunodeficienc...

journal_title：Journal of clinical microbiology

authors： Tintelnot K,Haase G,Seibold M,Bergmann F,Staemmler M,Franz T,Naumann D

更新日期：2000-04-01 00:00:00

abstract：:A new anti-human immunodeficiency virus type 1 and 2 (anti-HIV 1 and 2) test is described. It uses recombinant p24 and peptides covering gp32, gp41, and gp120 to identify HIV-1 and HIV-2 infections. This test has been shown to be specific (99.5%) and sensitive (99.8%). In this respect, the assay was equal or superior ...

journal_title：Journal of clinical microbiology

authors： Hess G,Avillez F,Lourenco MH,D'Agostino F,Cambie G,Piot P,Vercauteren G,Michl U,Melchior W,Bayer H

更新日期：1994-02-01 00:00:00

abstract：:Because relatively few caprine arthritis-encephalitis virus (CAEV)-infected animals exhibit clinical signs of illness, efforts to control and eradicate this virus will depend heavily on a sensitive diagnostic test that can be easily carried out. The currently utilized tests are of limited usefulness because of relativ...

journal_title：Journal of clinical microbiology

authors： Archambault D,East N,Perk K,Dahlberg JE

更新日期：1988-05-01 00:00:00

abstract：:A novel panfungal PCR assay which detects the small-subunit rRNA gene sequence of the two major fungal organism groups was used to test whole-blood specimens obtained from a series of blood or bone marrow transplant recipients. The 580-bp PCR product was identified after amplification by panfungal primers and hybridiz...

journal_title：Journal of clinical microbiology

authors： Van Burik JA,Myerson D,Schreckhise RW,Bowden RA

更新日期：1998-05-01 00:00:00

abstract：:The DNA fingerprint profiles and somatic serotypes of 71 Pasteurella multocida capsule serogroup B isolates, 13 capsule serogroup E isolates, and 16 somatic reference serotype strains were compared. Each of the 16 reference somatic serotypes had a unique DNA fingerprint profile with the HhaI restriction endonuclease. ...

journal_title：Journal of clinical microbiology

authors： Wilson MA,Rimler RB,Hoffman LJ

更新日期：1992-06-01 00:00:00

abstract：:A TaqMan PCR was established for identification and quantitation of members of the Ehrlichia phagocytophila group in experimentally infected cows and in Ixodes ricinus ticks. The TaqMan PCR identified a 106-bp section of the 16S rRNA gene by use of a specific fluorogenic probe and two primers. This technique was speci...

journal_title：Journal of clinical microbiology

authors： Pusterla N,Huder JB,Leutenegger CM,Braun U,Madigan JE,Lutz H

更新日期：1999-05-01 00:00:00

abstract：:Variability of disease manifestations has been noted in patients with Lyme disease. A contributing factor to this variation may be the number of spirochetes present in infected patients. We evaluated clinical and laboratory findings for patients with erythema migrans with regard to the number of Borrelia burgdorferi o...

journal_title：Journal of clinical microbiology

authors： Liveris D,Wang G,Girao G,Byrne DW,Nowakowski J,McKenna D,Nadelman R,Wormser GP,Schwartz I

更新日期：2002-04-01 00:00:00

abstract：:Salmonella isolates have traditionally been classified by serotyping, the serologic identification of two surface antigens, O-polysaccharide and flagellin protein. Serotyping has been of great value in understanding the epidemiology of Salmonella and investigating disease outbreaks; however, production and quality con...

journal_title：Journal of clinical microbiology

authors： McQuiston JR,Parrenas R,Ortiz-Rivera M,Gheesling L,Brenner F,Fields PI

更新日期：2004-05-01 00:00:00

abstract：:Testing efforts for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been burdened by the scarcity of testing materials and personal protective equipment for health care workers. The simple and painless process of saliva collection allows for widespread testing, but enthusiasm is hampered by variable ...

journal_title：Journal of clinical microbiology

authors： Yee R,Truong TT,Pannaraj PS,Eubanks N,Gai E,Jumarang J,Turner L,Peralta A,Lee Y,Dien Bard J

更新日期：2021-01-21 00:00:00

abstract：:Taeniasis due to Taenia solium is a disease with important public health consequences, since the larval stage is not exclusive to the animal intermediate, the pig, but also infects humans, causing neurocysticercosis. Early diagnosis and treatment of T. solium tapeworm carriers is important to prevent human cysticercos...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Prendergast E,Castillo JP,Tinoco YO,Garcia HH,Gonzalez AE,Sterling CR,Cysticercosis Working Group in Peru.

更新日期：2008-01-01 00:00:00

abstract：:A total of 186 volunteers, including 40 hospital patients, participated in a cross-sectional survey of oropharyngeal colonization with Legionella pneumophila. Colonization was defined as the appearance of any L. pneumophila organisms on culture or a positive direct fluorescent-antibody (FA) test or both in the absence...

journal_title：Journal of clinical microbiology

authors： Bridge JA,Edelstein PH

更新日期：1983-11-01 00:00:00

abstract：:A human astrovirus (HAstV) strain from Kenya was characterized by nucleotide sequence analysis. Sequences from open reading frame 1a (ORF1a) clustered with genotype 6/7, those from ORF1b clustered with genotype 3, and those from ORF2 clustered with genotype 2. A recombination point in the ORF1b-ORF2 junction was ident...

journal_title：Journal of clinical microbiology

authors： Wolfaardt M,Kiulia NM,Mwenda JM,Taylor MB

更新日期：2011-02-01 00:00:00

abstract：:Gas-liquid chromatographic (GLC) profiles of cellular fatty acids and metabolic products were useful in identifying strains of Peptococcus saccharolyticus, Peptococcus asaccharolyticus, Peptostreptococcus anaerobius, Peptostreptococcus micros, and Streptococcus intermedius. The GLC results supported the recent taxonom...

journal_title：Journal of clinical microbiology

authors： Lambert MA,Armfield AY

更新日期：1979-10-01 00:00:00

abstract：:Human immunodeficiency virus type 1 serotype C was found in 545 of 712 Ethiopian patients by peptide enzyme immunoassay. Serotyping failed in 146 samples due to the absence of V3 antibodies or multiple reactivities. In 6 of 34 samples, discordant results were obtained by serotyping and genotyping, possibly due to dive...

journal_title：Journal of clinical microbiology

authors： Sherefa K,Sällberg M,Johansson B,Salminen M,Sönnerborg A

更新日期：1997-09-01 00:00:00

abstract：:Between September 1995 and August 1998, the incidence and diversity of the main human rotavirus genotypes (G1, G2, G3, and G4 and P[8], P[4], P[6], and P[9]) among Irish children were determined by using established and adapted reverse transcriptase PCR-based genotyping methods. From a total of 193 rotavirus-positive ...

journal_title：Journal of clinical microbiology

authors： O'Mahony J,Foley B,Morgan S,Morgan JG,Hill C

更新日期：1999-06-01 00:00:00

abstract：:Patients with infection from bacteremic Bartonella spp., tested using Bartonella Alphaproteobacteria growth medium (BAPGM), were retrospectively categorized into one of two groups that included those whose blood was collected once (group 1; n = 55) or three times (group 2; n = 36) within a 1-week period. Overall, 19 p...

journal_title：Journal of clinical microbiology

authors： Pultorak EL,Maggi RG,Mascarelli PE,Breitschwerdt EB

更新日期：2013-06-01 00:00:00

abstract：:Murine monoclonal antibodies (MAbs) against herpes simplex virus type 1 and 2 (HSV-1 and -2, respectively) nuclear antigens were used to identify cells infected with HSV-1 or -2 by indirect immunofluorescence in conjunction with flow cytometry after virus amplification of MRC-5 cell monolayers. The results indicate th...

journal_title：Journal of clinical microbiology

authors： McSharry JJ,Costantino R,McSharry MB,Venezia RA,Lehman JM

更新日期：1990-08-01 00:00:00

abstract：:The human cytomegalovirus (HCMV) a-sequence (a-seq) is located in the joining region between the long (L) and short (S) unique sequences of the virus (L-S junction), and this hypervariable junction has been used to differentiate HCMV strains. The purpose of this study was to investigate whether there are differences a...

journal_title：Journal of clinical microbiology

authors： Zaia JA,Gallez-Hawkins G,Churchill MA,Morton-Blackshere A,Pande H,Adler SP,Schmidt GM,Forman SJ

更新日期：1990-12-01 00:00:00

abstract：:Levels of amikacin in serum were determined in 106 serum specimens by a latex agglutination inhibition card test and by radioimmunoassay (RIA). Linear regression analysis demonstrated a high degree of correlation between the two assays (latex = 0.95 (RIA) + 0.69; r = 0.97). Assay of three control sera containing 7.5, ...

journal_title：Journal of clinical microbiology

authors： Khabbaz RF,Standiford HC,Bernstein D,Nipper HC,Tatem BA,Smalls U,Drusano GL,Caplan E

更新日期：1985-11-01 00:00:00

abstract：:Tropheryma whipplei, the agent of Whipple's disease, is a gram-positive rod-shaped bacterium that belongs to the group of actinobacteria. In order to produce monoclonal antibodies (MAbs) against this bacterium, we inoculated mice with two different strains, Slow2 and Endo5. We produced 13 and 10 MAbs against Slow2 and...

journal_title：Journal of clinical microbiology

authors： Yu Y,Kowalczewska M,Decloquement P,Nappez C,La Scola B

更新日期：2006-11-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the detection of circulating free and complexed staphylococcal antigens in various clinical categories of staphylococcal infections. Circulating immune complexes were studied by the polyethylene glycol precipitation method. Circulating immune complexes and staphyl...

journal_title：Journal of clinical microbiology

authors： Lentino JR,Rytel MW

更新日期：1982-12-01 00:00:00

abstract：:A mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis and its nonmucoid revertant were grown in a chemically defined alginate-promoting medium under batch and continuous culture conditions. Selection for the mucoid and nonmucoid phenotype was accomplished by varying the levels of air a...

journal_title：Journal of clinical microbiology

authors： Krieg DP,Bass JA,Mattingly SJ

更新日期：1986-12-01 00:00:00

abstract：:West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia crocidurae, had never been cultured in vitro. TBRF was rapidly diagnosed for two patients returning from western Africa with fev...

journal_title：Journal of clinical microbiology

authors： van Dam AP,van Gool T,Wetsteyn JC,Dankert J

更新日期：1999-06-01 00:00:00

abstract：:We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HMA) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes. ...

journal_title：Journal of clinical microbiology

authors： Ito K,Iida M,Yamazaki M,Moriya K,Moroishi S,Yatsuyanagi J,Kurazono T,Hiruta N,Ratchtrachenchai OA

更新日期：2007-03-01 00:00:00

abstract：:A transferable plasmid encoding SHV-12 extended-spectrum beta-lactamase, TEM-116, and aminoglycoside resistance was responsible for two sequential clonal outbreaks of Enterobacter cloacae and Acinetobacter baumannii bacteria. A similar plasmid was present among isolates of four different bacterial species. Recognition...

journal_title：Journal of clinical microbiology

authors： Naiemi NA,Duim B,Savelkoul PH,Spanjaard L,de Jonge E,Bart A,Vandenbroucke-Grauls CM,de Jong MD

更新日期：2005-09-01 00:00:00

abstract：:Despite the high virological response rates achieved with current directly acting antiviral agents (DAAs) against hepatitis C virus (HCV), around 2% to 5% of treated patients do not achieve a sustained viral response. The identification of amino acid substitutions associated with treatment failure requires analytical ...

journal_title：Journal of clinical microbiology

authors： Soria ME,García-Crespo C,Martínez-González B,Vázquez-Sirvent L,Lobo-Vega R,de Ávila AI,Gallego I,Chen Q,García-Cehic D,Llorens-Revull M,Briones C,Gómez J,Ferrer-Orta C,Verdaguer N,Gregori J,Rodríguez-Frías F,Buti M,Este

更新日期：2020-11-18 00:00:00

abstract：:Two types of commercially available microdilution trays were inoculated with log-phase cultures, stationary-phase cultures, and direct suspensions of 18- to 24-h colonies, with essentially comparable results. Direct pick-up of colonies was also tested with two Prompt inoculation systems (3M Co.); they were found to be...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE,Hawkinson RW

更新日期：1983-09-01 00:00:00

abstract：:Phosphatase activity was found to be applicable as a criterion for the differentiation of Mycoplasma salivarium and Mycoplasma orale, predominant constituents of oral mycoplasmal flora. Therefore, a simple procedure for the phosphatase activity assay was established as a screening test for the differentiation of oral ...

journal_title：Journal of clinical microbiology

authors： Shibata K,Totsuka M,Watanabe T

更新日期：1986-05-01 00:00:00

abstract：:By the use of two reference procedures, an indirect hemagglutination assay and a complement fixation test, the presence or absence of cytomegalovirus (CMV) antibody was determined for 221 human sera. Ninety-nine sera (44.8%) were found to contain CMV antibody. The remaining 122 sera (55.2%) lacked detectable CMV antib...

journal_title：Journal of clinical microbiology

authors： Cheeseman SH,Doern GV,Ferriani RA,Keville MW,McGraw BR,Stewart JA

更新日期：1984-07-01 00:00:00