Oropharyngeal colonization with Legionella pneumophila.

abstract：:Six patients with bone marrow cultures yielding Mycobacterium avium complex were encountered at the Mount Sinai Hospital between 1969 and 1976. One additional isolate of the same mycobacterial species was recovered from splenic cyst fluid of a seventh patient. Because none of the patients had illnesses apparently due ...

journal_title：Journal of clinical microbiology

authors： Kozinn WP,Damsker B,Bottone EJ

更新日期：1980-03-01 00:00:00

abstract：:Serratia ficaria was first described in 1979 as part of the fig tree ecosystem (P.A.D. Grimont, F. Grimont, and M. P. Starr, Curr. Microbiol. 2:277-282, 1979). Since then, it has been isolated from clinical specimens from a few human patients (C. Bollet, J. Freney, P. de Micco, F. Grimont, and P.A.D. Grimont, Méd. Mal...

journal_title：Journal of clinical microbiology

authors： Darbas H,Jean-Pierre H,Paillisson J

更新日期：1994-09-01 00:00:00

abstract：:From 1974 to 1994, 2,033 specimens from children were submitted for cultures for anaerobic bacteria. Eighty-three Veillonella spp. were recovered from 83 children (4%). Most Veillonella species were recovered from abscesses, aspiration pneumonias, burns, bites, and sinuses. The infections were polymicrobial in 79 (95%...

journal_title：Journal of clinical microbiology

authors： Brook I

更新日期：1996-05-01 00:00:00

abstract：:Monoclonal antibody (MAb)-based sandwich direct enzyme-linked immunosorbent assay (MSD-ELISA) methods that can detect foot-and-mouth disease virus (FMDV) antigens, both multiserotype (MSD-ELISA/MS) (for O, A, C, and Asia 1) and single-serotype (MSD-ELISA/SS) (for O, A, and Asia 1, specifically), were developed. MAb 1H...

journal_title：Journal of clinical microbiology

authors： Morioka K,Fukai K,Yoshida K,Yamazoe R,Onozato H,Ohashi S,Tsuda T,Sakamoto K

更新日期：2009-11-01 00:00:00

abstract：:A total of 151 Escherichia coli strains resistant to cefotaxime and ceftazidime were isolated during a prospective surveillance study. These strains were characterized by clinical, microbiological, and molecular analyses and were distributed into four clusters of 103, 11, 6, and 5 isolates, along with 25 unrelated str...

journal_title：Journal of clinical microbiology

authors： Oteo J,Navarro C,Cercenado E,Delgado-Iribarren A,Wilhelmi I,Orden B,García C,Miguelañez S,Pérez-Vázquez M,García-Cobos S,Aracil B,Bautista V,Campos J

更新日期：2006-07-01 00:00:00

abstract：:Visceral and cutaneous leishmaniases are heterogenous entities. The Leishmania species that a given patient harbors usually cannot be determined clinically, and this identification is essential to prescribe the best species-specific therapeutic regimen. Our diagnosis procedure includes a real-time PCR assay targeted a...

journal_title：Journal of clinical microbiology

authors： Foulet F,Botterel F,Buffet P,Morizot G,Rivollet D,Deniau M,Pratlong F,Costa JM,Bretagne S

更新日期：2007-07-01 00:00:00

abstract：:We evaluated the diagnostic value of antibody to Blastomyces dermatitidis A precipitin arcs for identifying B. dermatitidis cultures by the exoantigen technique with 88 isolates from the United States and Canada, 12 from Africa, and 1 each from India and Israel. In addition, we studied 190 mycelial-form isolates of ot...

journal_title：Journal of clinical microbiology

authors： Kaufman L,Standard PG,Weeks RJ,Padhye AA

更新日期：1983-07-01 00:00:00

abstract：:Human infections with Salmonella enterica subspecies enterica serovar Senftenberg are often associated with exposure to poultry flocks, farm environments, or contaminated food. The recent emergence of multidrug-resistant isolates has raised public health concerns. In this study, comparative genomics and phenotypic ana...

journal_title：Journal of clinical microbiology

authors： Abd El Ghany M,Shi X,Li Y,Ansari HR,Hill-Cawthorne GA,Ho YS,Naeem R,Pickard D,Klena JD,Xu X,Pain A,Hu Q

更新日期：2016-08-01 00:00:00

abstract：:Pluronic polyols markedly improved the growth of two human lymphocyte cell lines when added to the growth medium in concentrations of 0.05 to 0.1%. The results of the current studies suggest that, in addition to the protective effect of polyols against mechanical damage of mammalian cells in submerged cultures, the pl...

journal_title：Journal of clinical microbiology

authors： Mizrahi A

更新日期：1975-07-01 00:00:00

abstract：:Restriction fragment length polymorphism (RFLP) analysis with probes derived from the insertion element IS6110, the direct repeat sequence, and the polymorphic GC-rich sequence (PGRS) and a PCR-based typing method called spacer oligonucleotide typing (spoligotyping) were used to strain type Mycobacterium bovis isolate...

journal_title：Journal of clinical microbiology

authors： Costello E,O'Grady D,Flynn O,O'Brien R,Rogers M,Quigley F,Egan J,Griffin J

更新日期：1999-10-01 00:00:00

abstract：:A microwave-accelerated direct immunofluorescence staining method which requires only 20 min from specimen receipt to interpretation is as effective as conventional methods for detecting respiratory syncytial virus and influenza A virus antigens in clinical specimens. The time required compares favorably with that for...

journal_title：Journal of clinical microbiology

authors： Hite SA,Huang YT

更新日期：1996-07-01 00:00:00

abstract：:We examined mutations in the dihydropteroate synthase (DHPS) genes of Pneumocystis carinii f. sp. hominis (P. carinii) strains isolated from 24 patients with P. carinii pneumonia (PCP) in Japan. DHPS mutations were identified at amino acid positions 55 and/or 57 in isolates from 6 (25.0%) of 24 patients. The underlyin...

journal_title：Journal of clinical microbiology

authors： Takahashi T,Hosoya N,Endo T,Nakamura T,Sakashita H,Kimura K,Ohnishi K,Nakamura Y,Iwamoto A

更新日期：2000-09-01 00:00:00

abstract：:Malaria-specific rapid diagnostic tests (RDTs) targeting aldolase show highly variable sensitivities. We assessed diversity in Plasmodium falciparum and P. vivax aldolases by sequencing the coding genes from parasites of various origins. The results show that aldolases are highly conserved, indicating that antigenic d...

journal_title：Journal of clinical microbiology

authors： Lee N,Baker J,Bell D,McCarthy J,Cheng Q

更新日期：2006-12-01 00:00:00

abstract：:Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures were recovered and examined for LT production. Preservation of the cultures on Dorset Egg Medium at 4 degrees C and pre...

journal_title：Journal of clinical microbiology

authors： Yoh M,Narita I,Honda T,Miwatani T,Nishibuchi M

更新日期：1991-10-01 00:00:00

abstract：:This report describes the first well-documented reported case of the recovery of Blastomyces dermatitidis from the blood of a patient with disseminated blastomycosis. The isolate was recovered by using a lysis-centrifugation method and inhibitory mold agar after 6 days of incubation at 30 degrees C. One of six blood s...

journal_title：Journal of clinical microbiology

authors： Musial CE,Wilson WR,Sinkeldam IR,Roberts GD

更新日期：1987-08-01 00:00:00

abstract：:The slightly revised, current scheme of 20 flagellar (H) antigens of Serratia marcesens was examined. The seven new H antigens were demonstrated to be antigenically distinct as determined with Le Minor's H-immobilization test. The H-immobilization antibodies of rabbit anti-H immune sera proved resistant to treatment w...

journal_title：Journal of clinical microbiology

authors： Traub WH,Fukushima PI

更新日期：1979-07-01 00:00:00

abstract：:To apply cDNA-RNA hybridization methods to the detection of hepatitis A virus (HAV) in clinical materials, we developed a two-step method in which a microtiter-based, solid-phase immunoadsorption procedure incorporating a monoclonal anti-HAV capture antibody was followed by direct blotting of virus eluates to nitrocel...

journal_title：Journal of clinical microbiology

authors： Jansen RW,Newbold JE,Lemon SM

更新日期：1985-12-01 00:00:00

abstract：:Total immunoglobulin E (IgE) and specific IgE were assayed by a radioimmunosorbent test and a reverse enzyme immunoassay in patients with hookworm infections before and after treatment. A total of 77 subjects (30 patients with hookworm infections and 47 subjects as controls) were studied. Both specific and total IgE l...

journal_title：Journal of clinical microbiology

authors： Ganguly NK,Mahajan RC,Sehgal R,Shetty P,Dilawari JB

更新日期：1988-04-01 00:00:00

abstract：:The main goal of this study was to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA), particularly livestock-associated MRSA (LA-MRSA) in pigs and pork. The genotypic relatedness of isolates on the farm, at slaughter, and at the retail level was assessed. Paired nasal and perianal swab sa...

journal_title：Journal of clinical microbiology

authors： Molla B,Byrne M,Abley M,Mathews J,Jackson CR,Fedorka-Cray P,Sreevatsan S,Wang P,Gebreyes WA

更新日期：2012-11-01 00:00:00

abstract：:Bacteroides forsythus has been associated with destructive adult periodontitis. Up to now, detailed analysis by classical means was hampered by the fastidious nature of the organism. There is hope that the application of molecular detection methods such as indirect immunofluorescence or in situ hybridization (ISH) wil...

journal_title：Journal of clinical microbiology

authors： Gersdorf H,Meissner A,Pelz K,Krekeler G,Göbel UB

更新日期：1993-04-01 00:00:00

abstract：:The 30-micrograms cefprozil disk and the cephalosporin class (30-micrograms cephalothin) disk were compared for their abilities to predict cefprozil susceptibility by agar disk diffusion testing. High error (5.02% major and 14.11% minor) rates were encountered with the cephalothin disk and were most frequently observe...

journal_title：Journal of clinical microbiology

authors： Fung-Tomc J,Stickle T,Doyle C,Huczko E,Kessler RE

更新日期：1991-11-01 00:00:00

abstract：:Shortly after adopting a 6-week-old cat, a veterinarian was bitten on the left index finger. Within 3 weeks, he developed headache, fever, and left axillary lymphadenopathy. Initial blood cultures from the cat and veterinarian were sterile. Repeat cultures from the cat grew Bartonella-like organisms with lophotrichous...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Hilyard EJ,Hadfield TL,Wilson KH,Steigerwalt AG,Brenner DJ,Breitschwerdt EB

更新日期：1997-07-01 00:00:00

abstract：:Vibrio vulnificus was isolated from a bacteremic patient. This strain, together with other isolates of V. vulnificus, was compared with V. alginolyticus, V. fluvialis, and V. parahaemolyticus with regard to growth characteristics on enteric agar media (enabling isolation and identification) and production of exoenzyme...

journal_title：Journal of clinical microbiology

authors： Desmond EP,Janda JM,Adams FI,Bottone EJ

更新日期：1984-02-01 00:00:00

abstract：:Direct heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosis isolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, w...

journal_title：Journal of clinical microbiology

authors： Saribaş Z,Kocagöz T,Alp A,Günalp A

更新日期：2003-02-01 00:00:00

abstract：:In this study, 5-iodo-2'-deoxyuridine-, cytochalasin B-, and cycloheximide-treated and untreated McCoy cells were inoculated with representative strains of Chlamydia trachomatis serotypes, as well as with clinical specimens from a pediatric population, and the quality and quantity of the cytoplasmic inclusions were ex...

journal_title：Journal of clinical microbiology

authors： La Scolea LJ Jr,Keddell JE

更新日期：1981-04-01 00:00:00

abstract：:Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct gram-negative bacteria. IEM should be a valuable tool for confirming presumptive isolates of F. tularensis and may potent...

journal_title：Journal of clinical microbiology

authors： Geisbert TW,Jahrling PB,Ezzell JW Jr

更新日期：1993-07-01 00:00:00

abstract：:Panels of hepatitis B virus surface antigen-positive sera from drug abusers were used to evaluate 14 commercial enzyme immunoassays from six companies for detecting hepatitis delta virus (HDV) markers. For detecting hepatitis delta virus antigen (HDAg), the Wellcome, Pasteur and Noctech assays had 100% sensitivity for...

journal_title：Journal of clinical microbiology

authors： Shattock AG,Morris MC

更新日期：1991-09-01 00:00:00

abstract：:Candida auris is an emerging worldwide fungal pathogen. Over the past 20 years, 61 patient isolates of C. auris (4 blood and 57 ear) have been obtained from 13 hospitals in Korea. Here, we reanalyzed those molecularly identified isolates using two matrix-assisted laser desorption ionization-time of flight mass spectro...

journal_title：Journal of clinical microbiology

authors： Kwon YJ,Shin JH,Byun SA,Choi MJ,Won EJ,Lee D,Lee SY,Chun S,Lee JH,Choi HJ,Kee SJ,Kim SH,Shin MG

更新日期：2019-03-28 00:00:00

abstract：:Helcococcus kunzii, a nonvirulent member of the human skin flora, has recently been implicated in causing infections in immunosuppressed patients. We report a case of breast abscess associated with H. kunzii in an immunocompetant patient and discuss the criteria used in its identification and our observations of susce...

journal_title：Journal of clinical microbiology

authors： Chagla AH,Borczyk AA,Facklam RR,Lovgren M

更新日期：1998-08-01 00:00:00

abstract：:A fecal PCR assay for detection of Helicobacter infections in laboratory rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not require euthanasia of ro...

journal_title：Journal of clinical microbiology

authors： Beckwith CS,Franklin CL,Hook RR Jr,Besch-Williford CL,Riley LK

更新日期：1997-06-01 00:00:00