Efficacy of various cell culture procedures for detection of Chlamydia trachomatis and applicability to diagnosis of pediatric infections.

abstract：:An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. Th...

journal_title：Journal of clinical microbiology

authors： Wallis C,Melnick JL,Longoria CJ

更新日期：1981-09-01 00:00:00

abstract：:Twenty-three young adult rhesus monkeys from China were evaluated for the presence of Helicobacter pylori. Gastric body and antral biopsy samples were tested for H. pylori by PCR analysis, culture, rapid urease testing, and histologic evaluation. Serologic testing to detect H. pylori immunoglobulin G (IgG) antibodies ...

journal_title：Journal of clinical microbiology

authors： Handt LK,Fox JG,Yan LL,Shen Z,Pouch WJ,Ngai D,Motzel SL,Nolan TE,Klein HJ

更新日期：1997-01-01 00:00:00

abstract：:Phenolphthalein diphosphate was incorporated into a primary blood agar medium for use in performing quantitative urine cultures. Phosphatase-negative staphylococci, such as Staphylococcus saprophyticus, were differentiated from phosphatase-positive species, such as Staphylococcus epidermidis, by spot testing colonies ...

journal_title：Journal of clinical microbiology

authors： Pickett DA,Welch DF

更新日期：1985-03-01 00:00:00

abstract：:A cluster of Mycobacterium ulcerans infections has recently occurred on Phillip Island, Victoria, Australia. Previous cases of infection have generally been located around Bairnsdale in southeast Gippsland. The aim of this study was to determine the epidemiological relationship between these strains and other strains ...

journal_title：Journal of clinical microbiology

authors： Jackson K,Edwards R,Leslie DE,Hayman J

更新日期：1995-09-01 00:00:00

abstract：:Shiga toxin-producing Escherichia coli (STEC) in northern Alberta was detected using two enzyme immunoassays and an in-house real-time PCR. Of 2,328 stool samples, 8 were positive for O157:H7 STEC and 13 were positive for non-O157 STEC. No significant gender (P = 0.17) or age (P = 0.81) differences between groups were...

journal_title：Journal of clinical microbiology

authors： Chui L,Lee MC,Malejczyk K,Lim L,Fok D,Kwong P

更新日期：2011-12-01 00:00:00

abstract：:Investigations of nursery outbreaks of Citrobacter diversus sepsis and meningitis have been hampered by lack of adequate epidemiologic markers for the organism. We studied outer membrane protein profiles from clinical isolates of C. diversus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whe...

journal_title：Journal of clinical microbiology

authors： Kline MW,Mason EO Jr,Kaplan SL

更新日期：1989-08-01 00:00:00

abstract：:Using 920 sera, we compared the specificity and reproducibility of the hemagglutination treponemal test for syphilis with those of the fluorescent treponemal antibody-absorption test and the microhemagglutination assay for Treponema pallidum antibodies; we found all three tests to be comparable. However, the hemagglut...

journal_title：Journal of clinical microbiology

authors： Larsen SA,Hambie EA,Pettit DE,Perryman MW,Kraus SJ

更新日期：1981-10-01 00:00:00

abstract：:Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only serotype 9 rotavirus strains and reacted specifically with serotype 9 rotaviruses in an enzyme-linked immunosorbent assay...

journal_title：Journal of clinical microbiology

authors： Midthun K,Valdesuso J,Kapikian AZ,Hoshino Y,Green KY

更新日期：1989-09-01 00:00:00

abstract：:The comparison of Gram-stained urethral smears with Gen-Probe for the detection of Neisseria Gonorrhoeae in the urethras of males with symptomatic urethritis revealed a 99.6% correlation between the two methods. A simple Gram stain would appear to be the method of choice for the detection of gonorrhea in symptomatic m...

journal_title：Journal of clinical microbiology

authors： Juchau SV,Nackman R,Ruppart D

更新日期：1995-11-01 00:00:00

abstract：:This study demonstrates that significant reproducibility problems can occur during routine use of the Abbott Laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae. These problems can go undetected by the quality control procedures outlined in the manufacturer's package insert. We outline here proc...

journal_title：Journal of clinical microbiology

authors： Gronowski AM,Copper S,Baorto D,Murray PR

更新日期：2000-06-01 00:00:00

abstract：:Helcococcus kunzii, a nonvirulent member of the human skin flora, has recently been implicated in causing infections in immunosuppressed patients. We report a case of breast abscess associated with H. kunzii in an immunocompetant patient and discuss the criteria used in its identification and our observations of susce...

journal_title：Journal of clinical microbiology

authors： Chagla AH,Borczyk AA,Facklam RR,Lovgren M

更新日期：1998-08-01 00:00:00

abstract：:The detection of immunoglobulin M (IgM) antibodies to cytomegalovirus-induced late antigens by an indirect immunofluorescence assay was improved by the use of monoclonal antibodies to human IgM. Nonspecific background fluorescence was absent, facilitating the reading of the slides and the detection of a specific fluor...

journal_title：Journal of clinical microbiology

authors： Zerbini M,Musiani M,Gentilomi G,La Placa M

更新日期：1986-07-01 00:00:00

abstract：:The aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. One hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-...

journal_title：Journal of clinical microbiology

authors： Willinger B,Obradovic A,Selitsch B,Beck-Mannagetta J,Buzina W,Braun H,Apfalter P,Hirschl AM,Makristathis A,Rotter M

更新日期：2003-02-01 00:00:00

abstract：:Recent studies have resulted in the proposal of a new species, Aeromonas schubertii (mannitol, sucrose, and indole negative), formerly termed Enteric Group 501, on the basis of the study of seven strains isolated from the southeastern and southwestern United States and Puerto Rico. We have isolated two phenotypically ...

journal_title：Journal of clinical microbiology

authors： Carnahan AM,Marii MA,Fanning GR,Pass MA,Joseph SW

更新日期：1989-08-01 00:00:00

abstract：:As microbial genomics makes increasingly important contributions to clinical and public health microbiology, the interpretation of whole-genome sequence data by nonspecialists becomes essential. In the absence of capsule-based vaccines, two protein-based vaccines have been used for the prevention of invasive serogroup...

journal_title：Journal of clinical microbiology

authors： Rodrigues CMC,Jolley KA,Smith A,Cameron JC,Feavers IM,Maiden MCJ

更新日期：2020-12-17 00:00:00

abstract：:Molecular methodologies were used to identify 28 Achromobacter spp. from patients with cystic fibrosis (CF). Multilocus sequence typing (MLST) identified 17 Achromobacter xylosoxidans isolates (all bla(OXA-114) positive), nine Achromobacter ruhlandii isolates (all bla(OXA-114) positive), one Achromobacter dolens isola...

journal_title：Journal of clinical microbiology

authors： Rodrigues ER,Ferreira AG,Leão RS,Leite CC,Carvalho-Assef AP,Albano RM,Marques EA

更新日期：2015-12-01 00:00:00

abstract：:An apparent outbreak of serious Candida albicans infections (n = 6) occurred in a surgical intensive care unit over a 4-week period. Four patients developed C. albicans bloodstream infections. An additional patient developed catheter-related C. albicans infection; the sixth patient developed an infection of cerebrospi...

journal_title：Journal of clinical microbiology

authors： Voss A,Pfaller MA,Hollis RJ,Rhine-Chalberg J,Doebbeling BN

更新日期：1995-03-01 00:00:00

abstract：:Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative ...

journal_title：Journal of clinical microbiology

authors： Titze-de-Almeida R,Willems RJ,Top J,Rodrigues IP,Ferreira RF 2nd,Boelens H,Brandileone MC,Zanella RC,Felipe MS,van Belkum A

更新日期：2004-10-01 00:00:00

abstract：:An alpha-toxin disk test is described in which group B streptococci completed the hemolysis of sheep erythrocytes partially lysed by the alpha-toxin of Clostridium perfringens. The test was performed satisfactorily on the sheep blood agar primary isolation plate, as well as on pure cultures. A total of 95% of strains ...

journal_title：Journal of clinical microbiology

authors： Smith JA,Ngui-Yen JH

更新日期：1980-07-01 00:00:00

abstract：:Murine monoclonal antibodies (MAbs) were produced which were specific for Pasteurella haemolytica serotype 1 lipopolysaccharide (LPS). The MAbs also reacted with LPS present in a partially purified antigen derived from a saline extract of the organism. The epitope to which the MAbs were directed was a carbohydrate whi...

journal_title：Journal of clinical microbiology

authors： Durham JA,Antone SM,Cunningham MW,Confer AW

更新日期：1988-05-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for detection of feline leukemia virus (FeLV) p27 in saliva was tested for its accuracy and sensitivity in diagnosing FeLV infections. Saliva and serum samples from 564 clinical cases were tested with a 99.2% specificity. The overall accuracy of the saliva ELISA reactive to...

journal_title：Journal of clinical microbiology

authors： Lewis MG,Wright KA,Lafrado LJ,Shanker PJ,Palumbo NE,Lemoine ED,Olsen RG

更新日期：1987-07-01 00:00:00

abstract：:We isolated and characterized a Vibrio cholerae O139 Bengal-specific DNA region by arbitrary PCR. The fragment contains open reading frames encoding two potential glycosyltransferases possibly involved in capsular polysaccharide or lipopolysaccharide biosynthesis. In order to evaluate the possibility that this region ...

journal_title：Journal of clinical microbiology

authors： Falklind S,Stark M,Albert MJ,Uhlén M,Lundeberg J,Weintraub A

更新日期：1996-12-01 00:00:00

abstract：:Accurate identification of pathogenic species is important for early appropriate patient management, but growing diversity of infectious species/strains makes the identification of clinical yeasts increasingly difficult. Among conventional methods that are commercially available, the API ID32C, AuxaColor, and Vitek 2 ...

journal_title：Journal of clinical microbiology

authors： Posteraro B,Efremov L,Leoncini E,Amore R,Posteraro P,Ricciardi W,Sanguinetti M

更新日期：2015-08-01 00:00:00

abstract：:Herpes simplex virus (HSV) causes acute and relapsing symptoms characterized by ulcerative lesions. Laboratory diagnosis of HSV in cutaneous or mucocutaneous lesions has historically been performed with the use of viral cell culture systems; however, these tests are laborious and suffer decreased sensitivity for advan...

journal_title：Journal of clinical microbiology

authors： Faron ML,Ledeboer NA,Patel A,Beqa SH,Yen-Lieberman B,Kohn D,Leber AL,Mayne D,Northern WI,Buchan BW

更新日期：2016-08-01 00:00:00

abstract：:CDC eugonic oxidizer group 2 (EO-2) is a group of unclassified gram-negative bacterial strains isolated from various human sources. As determined by biochemical tests and analyses of fatty acid compositions, these organisms form a homogeneous group that appears to be distinct from but related to other Paracoccus speci...

journal_title：Journal of clinical microbiology

authors： Daneshvar MI,Hollis DG,Weyant RS,Steigerwalt AG,Whitney AM,Douglas MP,Macgregor JP,Jordan JG,Mayer LW,Rassouli SM,Barchet W,Munro C,Shuttleworth L,Bernard K

更新日期：2003-03-01 00:00:00

abstract：:With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-...

journal_title：Journal of clinical microbiology

authors： Kessler JR,Kremer JR,Shulga SV,Tikhonova NT,Santibanez S,Mankertz A,Semeiko GV,Samoilovich EO,Tamfum JJ,Pukuta E,Muller CP

更新日期：2011-02-01 00:00:00

abstract：:A nontoxigenic strain of Vibrio cholerae O group 1 was isolated in Florida from the stool of a patient with severe diarrhea. The strain had the same hemolytic and unique phage-sensitivity pattern as all toxigenic isolates from recent cases of cholera in Texas and Louisiana. Identical strains were transiently isolated ...

journal_title：Journal of clinical microbiology

authors： Morris JG Jr,Picardi JL,Lieb S,Lee JV,Roberts A,Hood M,Gunn RA,Blake PA

更新日期：1984-02-01 00:00:00

abstract：:A total of 2,559 routine clinical specimens were cultured for mycobacteria by using BACTEC Middlebrook 7H12 medium (BACTEC), Lowenstein-Jensen slants (LJ), and Mycobactosel selective Middlebrook 7H11 slants (M7H11). Thirty-three isolates (1.3%) of M. avium complex and 82 isolates (3.2%) of M. tuberculosis were recover...

journal_title：Journal of clinical microbiology

authors： Kirihara JM,Hillier SL,Coyle MB

更新日期：1985-11-01 00:00:00

abstract：:Salmonella enterica serotype Typhi clinical isolates (n = 91) resistant to nalidixic acid (Nal(r)) were collected from sporadic cases and minor outbreaks throughout Vietnam between 1996 and 2004. These isolates were typed and compared by four methods: Vi phage typing, PstI ribotyping, XbaI and SpeI pulsed-field gel el...

journal_title：Journal of clinical microbiology

authors： Le TA,Fabre L,Roumagnac P,Grimont PA,Scavizzi MR,Weill FX

更新日期：2007-11-01 00:00:00

abstract：:We report on a case of phaeohyphomycosis caused by Alternaria infectoria in a renal transplant recipient with pulmonary infiltrates and multiple skin lesions. Diagnosis was based on microscopy and culture of the skin lesions. Treatment consisted of a combination of surgical excision and systemic antifungal therapy, fi...

journal_title：Journal of clinical microbiology

authors： Halaby T,Boots H,Vermeulen A,van der Ven A,Beguin H,van Hooff H,Jacobs J

更新日期：2001-05-01 00:00:00