Epidemiologic marker system for Citrobacter diversus using outer membrane protein profiles.

abstract：:The genetic and phylogenetic characteristics of human enterovirus 71 (EV71) and coxsackievirus A16 (CA16) sampled from children with hand, foot, and mouth disease in Shenzhen, People's Republic of China, over a 6-year period (1999 to 2004) were examined with reverse transcription-PCR and DNA sequencing. Out of 147 sto...

journal_title：Journal of clinical microbiology

authors： Li L,He Y,Yang H,Zhu J,Xu X,Dong J,Zhu Y,Jin Q

更新日期：2005-08-01 00:00:00

abstract：:Thirty stool filtrates known to contain Clostridium difficile toxin based on previous testing on McCoy cells were tested for toxicity on primary African green monkey kidney (AGMK), McCoy, MRC-5, primary rhesus monkey kidney (RMK), and Vero cells. All 30 filtrates showed cytotoxic effect at greater than or equal to 1:1...

journal_title：Journal of clinical microbiology

authors： Maniar AC,Williams TW,Hammond GW

更新日期：1987-10-01 00:00:00

abstract：:Human and porcine enterotoxigenic strains of Escherichia coli were cultivated in tryptone-yeast extract medium or brain heart infusion broth and tested for production of heat-labile enterotoxin by the GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA) and the Y1 adrenal cell assay. When testing for enteroto...

journal_title：Journal of clinical microbiology

authors： Gustafsson B,Möllby R

更新日期：1982-02-01 00:00:00

abstract：:A total of 215 typical strains of Candida albicans were studied for their ability to assimilate 11 carbon compounds. All isolates assimilated lactic acid, ribitol, succinic acid, methyl alpha-D-glucopyranoside. None of the isolates assimilated cellobiose and salicin; 1.9% of the isolates assimilated L-arabinose. Citri...

journal_title：Journal of clinical microbiology

authors： Syverson RE

更新日期：1981-01-01 00:00:00

abstract：:We demonstrate that all nine species of the Burkholderia cepacia complex can express the mucoid phenotype. A survey of clinical isolates showed that strains of B. cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can conve...

journal_title：Journal of clinical microbiology

authors： Zlosnik JE,Hird TJ,Fraenkel MC,Moreira LM,Henry DA,Speert DP

更新日期：2008-04-01 00:00:00

abstract：:Between November 1983 and January 1984, 13 cases of Candida parapsilosis endophthalmitis occurred in Florida, Georgia, and Tennessee in patients who had had an intraocular lens implantation (IOLI) or cataract extraction with an IOLI. This outbreak followed the introduction in July 1983 of a new brand of balanced salt ...

journal_title：Journal of clinical microbiology

authors： McCray E,Rampell N,Solomon SL,Bond WW,Martone WJ,O'Day D

更新日期：1986-10-01 00:00:00

abstract：:From late 2003 to the end of 2005, the Health Protection Agency's national reference laboratories received approximately 1,600 referrals of Acinetobacter spp., including 419 and 58 examples, respectively, of two carbapenem-resistant Acinetobacter baumannii lineages, designated OXA-23 clones 1 and 2. Representatives of...

journal_title：Journal of clinical microbiology

authors： Coelho JM,Turton JF,Kaufmann ME,Glover J,Woodford N,Warner M,Palepou MF,Pike R,Pitt TL,Patel BC,Livermore DM

更新日期：2006-10-01 00:00:00

abstract：:In order to better understand the role of Mycobacterium leprae nasal carriage in the maintenance of infection reservoirs and transmission of leprosy, we applied a polymerase chain reaction (PCR) that detected a 531-bp fragment of the pra gene of M. leprae on nasal swab specimens collected through a total population su...

journal_title：Journal of clinical microbiology

authors： Klatser PR,van Beers S,Madjid B,Day R,de Wit MY

更新日期：1993-11-01 00:00:00

abstract：:Fifty staphylococcal strains that produced toxic shock syndrome (TSS) toxin 1 and that were isolated from patients with TSS were characterized. One strain had more properties that were characteristic of Staphylococcus hyicus than of Staphylococcus aureus. Forty-four strains had the same properties or differed in only ...

journal_title：Journal of clinical microbiology

authors： Aliu B,Bergdoll MS

更新日期：1988-11-01 00:00:00

abstract：:A rapid and accurate method to identify the species and antibiotic resistance of Candida spp. in blood is vital to increase the survival rates of patients with bloodstream infections. However, the extremely low levels of Candida spp. in blood make rapid diagnosis by standard blood culture difficult. In this study, we ...

journal_title：Journal of clinical microbiology

authors： Chen XL,Zheng H,Li WG,Zhong YH,Chen XP,Lu JX

更新日期：2020-03-25 00:00:00

abstract：:Taeniasis due to Taenia solium is a disease with important public health consequences, since the larval stage is not exclusive to the animal intermediate, the pig, but also infects humans, causing neurocysticercosis. Early diagnosis and treatment of T. solium tapeworm carriers is important to prevent human cysticercos...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Prendergast E,Castillo JP,Tinoco YO,Garcia HH,Gonzalez AE,Sterling CR,Cysticercosis Working Group in Peru.

更新日期：2008-01-01 00:00:00

abstract：:The Kodak SureCell Herpes Test Kit (SC-HSV) was evaluated in a high-risk sexually transmitted disease clinic population. A total of 97 lesion specimens (from 94 patients) were tested for the presence of herpes simplex virus by SC-HSV and by spin-amplified tissue culture confirmed by an enzyme immunoassay. The overall ...

journal_title：Journal of clinical microbiology

authors： Dorian KJ,Beatty E,Atterbury KE

更新日期：1990-09-01 00:00:00

abstract：:MARC-145 cell line is commonly used to isolate porcine reproductive and respiratory syndrome virus (PRRSV) for diagnostics, research and vaccine production, but it yields frustratingly low success rates of virus isolation (VI). ZMAC cell line, derived from porcine alveolar macrophages, has become available but its uti...

journal_title：Journal of clinical microbiology

authors： Yim-Im W,Huang H,Park J,Wang C,Calzada G,Gauger P,Harmon K,Main R,Zhang J

更新日期：2020-12-02 00:00:00

abstract：:We recently tested serum from a human immunodeficiency virus-infected patient for the presence of cryptococcal antigen using the Meridian latex agglutination (LA) test (Cryptococcal Antigen Latex Agglutination System). Two pronase-treated serum specimens from the patient had LA titers of 80 and 160, but the patient ha...

journal_title：Journal of clinical microbiology

authors： Whittier S,Hopfer RL,Gilligan P

更新日期：1994-09-01 00:00:00

abstract：:The majority of cervical human papillomavirus (HPV) infections in young women are transient, but whether the clearance differs among different HPV genotypes and the different factors predicting genotype-specific clearance are partly unknown. In the Finnish Family HPV Study, 131 of 252 women (mean age, 25.5 years) clea...

journal_title：Journal of clinical microbiology

authors： Louvanto K,Syrjänen KJ,Rintala MA,Grénman SE,Syrjänen SM

更新日期：2010-08-01 00:00:00

abstract：:Norwalk virus (NV) and the Norwalk-like viruses are important human pathogens that cause epidemic acute viral gastroenteritis. Current techniques used to recover NV from clinical samples involve multistep viral extraction and elution procedures with subsequent viral detection by reverse transcription-PCR (RT-PCR). In ...

journal_title：Journal of clinical microbiology

authors： Schwab KJ,Estes MK,Neill FH,Atmar RL

更新日期：1997-02-01 00:00:00

abstract：:This study demonstrates the dynamics in the epidemiology of hepatitis C virus subtypes. Subtypes 3a and 4a have become increasingly prevalent in patients where an infection within recent years can be assumed. Evidence is presented that the subtypes observed among younger patients can spread rapidly and lead to signifi...

journal_title：Journal of clinical microbiology

authors： Schröter M,Zöllner B,Schäfer P,Reimer A,Müller M,Laufs R,Feucht HH

更新日期：2002-05-01 00:00:00

abstract：:Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was collected from children with bullous impetigo in 2003 and 2004. One strain collected in 2003 was Panton-Valentine leucocidin (PVL) positive. In 2004, a multiple-drug-resistant PVL(+) CA-MRSA strain was isolated from an athlete with a cutaneou...

journal_title：Journal of clinical microbiology

authors： Takizawa Y,Taneike I,Nakagawa S,Oishi T,Nitahara Y,Iwakura N,Ozaki K,Takano M,Nakayama T,Yamamoto T

更新日期：2005-07-01 00:00:00

abstract：:The use of real-time quantitative PCR (5' nuclease PCR assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. We developed primers and probes based on the 16S ribosomal DNA gene sequences for the detection of Escherichia coli and Bacteroides vulgatus. DNA was isolat...

journal_title：Journal of clinical microbiology

authors： Huijsdens XW,Linskens RK,Mak M,Meuwissen SG,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2002-12-01 00:00:00

abstract：:Imferon, an iron-dextran complex, enhances the growth of Neisseria gonorrhoeae and N. meningitidis. The use of Imferon as a replacement for ferric nitrate, in a defined supplement for GC agar significantly increased the average colony sizes of both gonococci and meningococci. In comparison with Thayer-Martin medium, I...

journal_title：Journal of clinical microbiology

authors： Payne SM,Finkelstein RA

更新日期：1977-09-01 00:00:00

abstract：:The ability of Yersinia to digest polypectate may be of some value in differentiating Y. enterocolitica and Y. pseudotuberculosis from some of the other fermenting gram-negative bacilli, such as Enterobacter agglomerans, with which they can be confused. Pectolytic activity in Yersinia may also have some teleologic or ...

journal_title：Journal of clinical microbiology

authors： von Riesen VL

更新日期：1975-12-01 00:00:00

abstract：:The replication of human coronavirus strain 229E was observed by using indirect immunofluorescence in infected monolayers of MRC continuous cells. By 8 h after infection, bright cytoplasmic fluorescence was detected in cells infected with human coronavirus 229E. Discrete foci of infection were observed from 8 to 16 h ...

journal_title：Journal of clinical microbiology

authors： Macnaughton MR,Thomas BJ,Davies HA,Patterson S

更新日期：1980-09-01 00:00:00

abstract：:The toluidine red unheated serum test (TRUST) antigen, a macroscopic flocculation test antigen developed by Pettit et al. (J. Clin. Microbiol. 18:1141-1145, 1983) by modifying the color-coded antigen of Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974), was compared with the Venereal Disease Research Laboratory...

journal_title：Journal of clinical microbiology

authors： Parham CE,Pettit DE,Larsen SA,Hambie EA,Perryman MW,McGrew BE

更新日期：1984-09-01 00:00:00

abstract：:Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural protein 3AB was used as the antigen in an enzyme-linked immunosorbent assay. This assay allowed the differentiation of vaccinated from infected pigs. Serial studies were performed using sera collected from pigs in the field. Positive reactions wer...

journal_title：Journal of clinical microbiology

authors： Chung WB,Sorensen KJ,Liao PC,Yang PC,Jong MH

更新日期：2002-08-01 00:00:00

abstract：:We described the molecular epidemiology of expanded-spectrum cephalosporin-resistant gram-negative bacilli (RGN) recovered from inanimate surfaces. RGN were isolated from 9% of environmental cultures. Numerous species, each with multiple unique strains, were recovered. Epidemiological links between environmental, pers...

journal_title：Journal of clinical microbiology

authors： D'Agata EM,Venkataraman L,DeGirolami P,Samore M

更新日期：1999-09-01 00:00:00

abstract：:Campylobacter rectus was isolated under routine anaerobic conditions (no additional hydrogen gas in the atmosphere) from an oral, nonperiodontal abscess from a patient with gastroesophageal adenocarcinoma. We report the first case of a palate abscess caused by C. rectus and review the literature and atmospheric requir...

journal_title：Journal of clinical microbiology

authors： Mahlen SD,Clarridge JE 3rd

更新日期：2009-03-01 00:00:00

abstract：:A newly developed enzyme immunoassay (EIA) was used to detect the presence of pneumococcal teichoic and lipoteichoic acids in cerebrospinal fluid (CSF) from patients with Streptococcus pneumoniae meningitis who were being treated with antibiotics. All initial CSF samples, which on culture grew S. pneumoniae, were posi...

journal_title：Journal of clinical microbiology

authors： Stuertz K,Merx I,Eiffert H,Schmutzhard E,Mäder M,Nau R

更新日期：1998-08-01 00:00:00

abstract：:We report a nonfatal case of streptococcal toxic shock syndrome (STSS) caused by a Streptococcus pyogenes emm118 strain encoding a novel variant of streptococcal mitogenic exotoxin Z (SMEZ-34). This variant was responsible for the major mitogenic activity in the cell culture supernatant. Patient sera showed seroconver...

journal_title：Journal of clinical microbiology

authors： Yang L,Thomas M,Woodhouse A,Martin D,Fraser JD,Proft T

更新日期：2005-07-01 00:00:00

abstract：:Isolation of Burkholderia pseudomallei currently relies on the use of Ashdown's selective agar (ASA). We designed a new selective agar (Burkholderia pseudomallei selective agar [BPSA]) to improve recovery of the more easily inhibited strains of B. pseudomallei. B. pseudomallei, Burkholderia cepacia, and Pseudomonas ae...

journal_title：Journal of clinical microbiology

authors： Howard K,Inglis TJ

更新日期：2003-07-01 00:00:00

abstract：:Aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. Sampling was by syringe aspiration. Strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. Randomly...

journal_title：Journal of clinical microbiology

authors： Williams BL,McCann GF,Schoenknecht FD

更新日期：1983-10-01 00:00:00