Enzyme immunoassay detecting teichoic and lipoteichoic acids versus cerebrospinal fluid culture and latex agglutination for diagnosis of Streptococcus pneumoniae meningitis.


:A newly developed enzyme immunoassay (EIA) was used to detect the presence of pneumococcal teichoic and lipoteichoic acids in cerebrospinal fluid (CSF) from patients with Streptococcus pneumoniae meningitis who were being treated with antibiotics. All initial CSF samples, which on culture grew S. pneumoniae, were positive in the EIA. A total of 14 subsequent culture-negative samples gave clear signals in the EIA up to day 15 after the onset of antibiotic treatment. For 11 CSF specimens, culture, microscopy, and latex agglutination were negative while the EIA detected pneumococcal antigens. The EIA did not react either with CSF of patients with meningitis caused by bacteria other than S. pneumoniae or by viral pathogens. In conclusion, this EIA can be a valuable tool for the diagnosis of S. pneumoniae meningitis from CSF samples in cases in which prior antimicrobial therapy minimizes the usefulness of culture or other antigen detection tests.


J Clin Microbiol


Stuertz K,Merx I,Eiffert H,Schmutzhard E,Mäder M,Nau R




Has Abstract


1998-08-01 00:00:00












  • Comparison of the Chinese schema and the International Antigenic Typing System for serotyping Pseudomonas aeruginosa.

    abstract::Twelve strains of Pseudomonas aeruginosa representing 12 serogroups in the serogrouping schema used in the People's Republic of China were compared with serogroups in the International Antigenic Typing System (IATS). The first eight groups originated in the People's Republic of China, and group II appears to have a ne...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Liu PV

    更新日期:1987-05-01 00:00:00

  • Comparison of identifications of human and animal source gram-negative bacteria by API 20E and crystal E/NF systems.

    abstract::This study compared the abilities of API 20E and BBL Crystal E/NF identification systems to correctly identify human and animal source gram-negative bacilli of known identifications, as provided by the American Type Culture Collection, Rockville, Md., and the Research Diagnostic and Investigative Laboratory, Columbia,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Peele D,Bradfield J,Pryor W,Vore S

    更新日期:1997-01-01 00:00:00

  • Use of PCR with feces for detection of Helicobacter pylori infections in patients.

    abstract::PCR was performed for the detection of Helicobacter pylori in feces from 24 patients with proven infections. Several precautions were taken to overcome possible inhibition of PCR with feces. In the first 12 patients, feces were examined shortly after endoscopy. In another group of 12 patients, who were treated during ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: van Zwet AA,Thijs JC,Kooistra-Smid AM,Schirm J,Snijder JA

    更新日期:1994-05-01 00:00:00

  • Distinct diversity of vacA, cagA, and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan.

    abstract::Colonization of the stomach mucosa by Helicobacter pylori is a major cause of acute and chronic gastric pathologies in humans. Several H. pylori virulence genes that may play a role in its pathogenicity have been identified. The most important determinants are vacA and cagA in the cag pathogenicity island (cagPAI) gen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Yamazaki S,Yamakawa A,Okuda T,Ohtani M,Suto H,Ito Y,Yamazaki Y,Keida Y,Higashi H,Hatakeyama M,Azuma T

    更新日期:2005-08-01 00:00:00

  • Development of a highly specific and sensitive rubella immunoglobulin M antibody capture enzyme immunoassay that uses enzyme-labeled antigen.

    abstract::An enzyme immunoassay (EIA) for serum immunoglobulin M (IgM) antibodies to rubella virus based on enzyme labeling of viral antigen was developed. The sensitivity of the EIA for the detection of recent rubella virus infection was evaluated by using 115 rubella-IgM-antibody-positive serum specimens, which were confirmed...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Seppänen H

    更新日期:1990-04-01 00:00:00

  • Validation of the INNO-LIA syphilis kit as a confirmatory assay for Treponema pallidum antibodies.

    abstract::The commercially available diagnostic tests for syphilis are mostly based on the use of extracted antigens of Treponema pallidum. Pronounced cross-reactivities with other spirochete antigens are often reported. The aim of this study was to validate a novel multiparametric assay (the assay performed with the kit) INNO-...

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章


    authors: Ebel A,Vanneste L,Cardinaels M,Sablon E,Samson I,De Bosschere K,Hulstaert F,Zrein M

    更新日期:2000-01-01 00:00:00

  • Identification of Pandoraea species by 16S ribosomal DNA-based PCR assays.

    abstract::The recently described genus Pandoraea contains five named species (Pandoraea apista, Pandoraea pulmonicola, Pandoraea pnomenusa, Pandoraea sputorum, and Pandoraea norimbergensis) and four unnamed genomospecies. Pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (CF) patients. Acc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Coenye T,Liu L,Vandamme P,LiPuma JJ

    更新日期:2001-12-01 00:00:00

  • Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis.

    abstract::Sepsis is a major health problem in newborns and children. Early detection of pathogens allows initiation of appropriate antimicrobial therapy that strongly correlates with positive outcomes. Multiplex PCR has the potential to rapidly identify bloodstream infections, compensating for the loss of blood culture sensitiv...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lucignano B,Ranno S,Liesenfeld O,Pizzorno B,Putignani L,Bernaschi P,Menichella D

    更新日期:2011-06-01 00:00:00

  • Evidence of a recombinant wild-type human astrovirus strain from a Kenyan child with gastroenteritis.

    abstract::A human astrovirus (HAstV) strain from Kenya was characterized by nucleotide sequence analysis. Sequences from open reading frame 1a (ORF1a) clustered with genotype 6/7, those from ORF1b clustered with genotype 3, and those from ORF2 clustered with genotype 2. A recombination point in the ORF1b-ORF2 junction was ident...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wolfaardt M,Kiulia NM,Mwenda JM,Taylor MB

    更新日期:2011-02-01 00:00:00

  • Epidemiologic marker system for Citrobacter diversus using outer membrane protein profiles.

    abstract::Investigations of nursery outbreaks of Citrobacter diversus sepsis and meningitis have been hampered by lack of adequate epidemiologic markers for the organism. We studied outer membrane protein profiles from clinical isolates of C. diversus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whe...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kline MW,Mason EO Jr,Kaplan SL

    更新日期:1989-08-01 00:00:00

  • Comparison of Gen-Probe DNA probe test and culture for the detection of Neisseria gonorrhoeae in endocervical specimens.

    abstract::A 2-h nonisotopic DNA probe assay for the direct detection of Neisseria gonorrhoeae in urogenital specimens has recently been modified (PACE 2; Gen-Probe, San Diego, Calif.). The new assay format was developed to increase the sensitivity of the assay and simplify procedural steps. In this study, the new DNA probe test...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Panke ES,Yang LI,Leist PA,Magevney P,Fry RJ,Lee RF

    更新日期:1991-05-01 00:00:00

  • Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens.

    abstract::A reverse transcription-seminested PCR (RT-snPCR) assay was developed for the detection and identification of enterovirus (EV) RNA in clinical specimens. Three conserved protein motifs were identified by aligning the VP3 and VP1 sequences of prototype EV strains. Consensus degenerate primers were designed from a conse...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Nix WA,Oberste MS,Pallansch MA

    更新日期:2006-08-01 00:00:00

  • Detection of influenza virus neuraminidase-specific antibodies by an enzyme-linked immunosorbent assay.

    abstract::An enzyme-linked immunosorbent assay was developed for the titration of antibodies in human sera to influenza virus neuraminidase, employing partially purified N1 neuraminidase. Specificity of the test was demonstrated, and the test was more sensitive than either the conventional neuraminidase inhibition or plaque siz...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Khan MW,Gallagher M,Bucher D,Cerini CP,Kilbourne ED

    更新日期:1982-07-01 00:00:00

  • Epidemiology of rotavirus electropherotypes determined by a simplified diagnostic technique with RNA analysis.

    abstract::The incidence and RNA electropherotypes of rotavirus in stools or rectal swabs of children with diarrhea were studied for three rotavirus seasons (1981 through 1984) in Philadelphia, Pa. We used a simplified RNA analysis method involving polyacrylamide gel electrophoresis followed by silver staining. Phosphate-buffere...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Dolan KT,Twist EM,Horton-Slight P,Forrer C,Bell LM Jr,Plotkin SA,Clark HF

    更新日期:1985-05-01 00:00:00

  • Testing for induction of clindamycin resistance in erythromycin-resistant isolates of Staphylococcus aureus.

    abstract::Disk diffusion and broth microdilution (BMD) were used to perform clindamycin (CLI) induction testing on 128 selected nonduplicate isolates of Staphylococcus aureus. Disk diffusion testing involved placing CLI and erythromycin (ERY) disks approximately 12 mm apart (measured edge to edge) on a Mueller-Hinton agar plate...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Steward CD,Raney PM,Morrell AK,Williams PP,McDougal LK,Jevitt L,McGowan JE Jr,Tenover FC

    更新日期:2005-04-01 00:00:00

  • New, extended biotyping scheme for Campylobacter jejuni, Campylobacter coli, and "Campylobacter laridis".

    abstract::A biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid H2S production, and DNA hydrolysis was applied to 1,826 cultures of Campylobacter jejuni, Campylobacter coli and "Campylobacter laridis" isolates from human and nonhuman sources. Four biotypes were identified among C. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lior H

    更新日期:1984-10-01 00:00:00

  • Rapid fingerprinting of Helicobacter pylori by polymerase chain reaction and restriction fragment length polymorphism analysis.

    abstract::A simple and reliable technique was developed for differentiating Helicobacter pylori strains by restriction fragment length polymorphism analysis of polymerase chain reaction (PCR)-amplified DNAs. Oligonucleotide primer pairs developed to the urease, 48-kDa stress protein (htrA), and 26-kDa antigen-encoding genes wer...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Clayton CL,Kleanthous H,Morgan DD,Puckey L,Tabaqchali S

    更新日期:1993-06-01 00:00:00

  • Real-time TaqMan PCR for quantifying oral bacteria during biofilm formation.

    abstract::A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems cons...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Suzuki N,Nakano Y,Yoshida A,Yamashita Y,Kiyoura Y

    更新日期:2004-08-01 00:00:00

  • Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens.

    abstract::The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13 influenza virus strains isolated from 390 clinical specimens, 9 (69%) were efficiently isolated by the centrifugation assay ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Seno M,Kanamoto Y,Takao S,Takei N,Fukuda S,Umisa H

    更新日期:1990-07-01 00:00:00

  • DNA macroarray for identification and typing of Staphylococcus aureus isolates.

    abstract::A DNA macroarray containing 465 intragenic amplicons was designed to identify Staphylococcus aureus at the species level and to type S. aureus isolates. The genes selected included those encoding (i) S. aureus-specific proteins, (ii) staphylococcal and enterococcal proteins mediating antibiotic resistance and factors ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Trad S,Allignet J,Frangeul L,Davi M,Vergassola M,Couve E,Morvan A,Kechrid A,Buchrieser C,Glaser P,El-Solh N

    更新日期:2004-05-01 00:00:00

  • Identification of Pneumocystis carinii f. sp. hominis gene sequences in filtered air in hospital environments.

    abstract::To evaluate the risk of a nosocomial spread of Pneumocystis carinii f. sp. hominis (P. carinii hominis), air filter samples from rooms of P. carinii pneumonia (PCP) patients, adjacent corridors, and other hospital environments have been investigated for the presence of P. carinii hominis. Amplified DNA from air filter...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Olsson M,Lidman C,Latouche S,Björkman A,Roux P,Linder E,Wahlgren M

    更新日期:1998-06-01 00:00:00

  • Mesenteric lymphadenitis of swine caused by Rhodococcus sputi.

    abstract::Rhodococcus sputi caused tuberculosislike lymphadenitis of mesenteric lymph nodes in swine. This is the first study reporting that R. sputi can be a pathogen in swine. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tsukamura M,Komatsuzaki C,Sakai R,Kaneda K,Kudo T,Seino A

    更新日期:1988-01-01 00:00:00

  • A serotype V clone is predominant among erythromycin-resistant Streptococcus agalactiae isolates in a southwestern region of Germany.

    abstract::One hundred ninety-three Streptococcus agalactiae isolates of neonatal origin and 146 isolates from adult women were analyzed for macrolide resistance and investigated for clonality. Among erythromycin-resistant isolates, serotype V turned out to be the most frequent. Comparative pulsed-field gel electrophoresis analy...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: von Both U,Ruess M,Mueller U,Fluegge K,Sander A,Berner R

    更新日期:2003-05-01 00:00:00

  • Survey of extended-spectrum beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae: prevalence of TEM-52 in Korea.

    abstract::Two hundred ninety isolates of Escherichia coli were investigated for the production of extended-spectrum beta-lactamases (ESBLs). Fourteen (4.8%) of the 290 strains were found to produce ESBLs. Each of the 14 strains produced one or two ESBLs, as follows: 10 strains produced TEM-52, 1 strain produced SHV-2a, 1 strain...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Pai H,Lyu S,Lee JH,Kim J,Kwon Y,Kim JW,Choe KW

    更新日期:1999-06-01 00:00:00

  • Drug-associated resistance mutations in plasma and peripheral blood mononuclear cells of human immunodeficiency virus type 1-infected patients for whom highly active antiretroviral therapy is failing.

    abstract::In 32 patients for whom highly active antiretroviral therapy was failing, a good agreement between drug resistance-associated mutations in plasma and peripheral blood mononuclear cells (PBMCs) was found (k = 0.85). The mutations with the lowest agreement were 20R, 63P, and 84V in the protease gene and 184V in the reve...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sarmati L,Nicastri E,Uccella I,D'Ettorre G,Parisi SG,Palmisano L,Galluzzo C,Concia E,Vullo V,Vella S,Andreoni M

    更新日期:2003-04-01 00:00:00

  • Mixed bacterial meningitis due to Streptococcus pneumoniae and Neisseria meningitidis in an 18-month-old child.

    abstract::We report an unusual case of culture-proven pneumococcal and meningococcal mixed meningitis in an 18-month-old girl. The patient responded well to antimicrobial therapy and recovered completely without sequelae. No underlying condition could be demonstrated except a rhinitis of unknown etiology 2 days before the onset...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审


    authors: Marchandin H,Ventura V,Alonso JM,Van de Perre P

    更新日期:2005-03-01 00:00:00

  • Detection of specific serum immunoglobulin M in nephropathia epidemica (Scandinavian epidemic nephropathy) by a biotin-avidin-amplified immunofluorescence method.

    abstract::A biotin-avidin-amplified indirect immunofluorescence method was used to demonstrate specific serum immunoglobulin M (IgM) antibodies in nephropathia epidemica, the Scandinavian type of hemorrhagic fever with renal syndrome. The antigen in the test was the cross-reacting agent of Korean hemorrhagic fever, Hantaan viru...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Settergren B,Juto P,Wadell G

    更新日期:1987-06-01 00:00:00

  • PCR of peripheral blood for diagnosis of meningococcal disease.

    abstract::Meningococcal disease is normally suspected on clinical grounds and is confirmed by isolation of Neisseria meningitidis bacteria from blood or cerebrospinal fluid or, more recently, by serology or PCR of cerebrospinal fluid. Achieving confirmation of a clinical diagnosis of meningococcal disease has become more diffic...

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章


    authors: Newcombe J,Cartwright K,Palmer WH,McFadden J

    更新日期:1996-07-01 00:00:00

  • Detection of Rift Valley fever virus antigen by enzyme-linked immunosorbent assay.

    abstract::A double-antibody (sandwich) enzyme-linked immunosorbent assay (ELISA) was adapted to detect Rift Valley fever virus antigen. Antibodies were purified from hyperimmune mouse and rabbit sera by affinity chromatography, using CNBr-activated Sepharose 4B coupled to a beta-propiolactone-inactivated sucrose-acetone-extract...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Niklasson B,Grandien M,Peters CJ,Gargan TP 2nd

    更新日期:1983-06-01 00:00:00

  • Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.

    abstract::Random amplified polymorphic DNA (RAPD) was used to better characterize the genotypic relatedness among medically important Candida species. By using short oligomer primers (10-mers) with arbitrarily chosen sequences in the polymerase chain reaction, distinctive and reproducible sets of polymerase chain reaction produ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lehmann PF,Lin D,Lasker BA

    更新日期:1992-12-01 00:00:00