Identification of Yersinia enterocolitica O15 lipopolysaccharide as a rough antigen.

abstract：:We report a case of disseminated Scedosporium/Pseudallescheria infection due to Pseudallescheria boydii sensu stricto after lung transplantation in a patient with cystic fibrosis. Dissemination occurred under voriconazole. Despite surgery and combination therapy with voriconazole, caspofungin, and terbinafine, the pat...

journal_title：Journal of clinical microbiology

authors： Morio F,Horeau-Langlard D,Gay-Andrieu F,Talarmin JP,Haloun A,Treilhaud M,Despins P,Jossic F,Nourry L,Danner-Boucher I,Pattier S,Bouchara JP,Le Pape P,Miegeville M

更新日期：2010-05-01 00:00:00

abstract：:An automated cultivation system for mycobacteria, the MGIT 960 system (MGIT system), was compared in the clinical routine with two variants of Löwenstein-Jensen (L-J) medium. A total of 152 isolates were recovered from 2,015 specimens: 139 (91%) with the MGIT system and 127 (84%) with L-J media (P = 0.05). These inclu...

journal_title：Journal of clinical microbiology

authors： Katila ML,Katila P,Erkinjuntti-Pekkanen R

更新日期：2000-03-01 00:00:00

abstract：:We standardized and assessed the performance of an in-house microtiter assay for determining the susceptibilities of Mycobacterium tuberculosis clinical isolates to isoniazid based on mycobacteriophage amplification technology. Seventy isolates (43 resistant and 27 sensitive according to the BACTEC 460 radiometric met...

journal_title：Journal of clinical microbiology

authors： Galí N,Domínguez J,Blanco S,Prat C,Alcaide F,Coll P,Ausina V

更新日期：2006-01-01 00:00:00

abstract：:Drug-resistant tuberculosis (TB) is a major threat to TB control worldwide. Globally, only 40% of the 340,000 notified TB patients estimated to have multidrug-resistant-TB (MDR-TB) were detected in 2015. This study was carried out to evaluate the utility of high-resolution melt curve analysis (HRM) for the rapid and d...

journal_title：Journal of clinical microbiology

authors： Anthwal D,Gupta RK,Bhalla M,Bhatnagar S,Tyagi JS,Haldar S

更新日期：2017-06-01 00:00:00

abstract：:Several studies have identified nonsterile blood collection tubes as the reservoir for contaminating microorganisms in outbreaks of pseudobacteremia. Microorganisms are thought to be transferred from the nonsterile tubes to the blood culture tubes when blood is drawn for cultures, but the validity of this hypothesis h...

journal_title：Journal of clinical microbiology

authors： McNeil MM,Davis BJ,Anderson RL,Martone WJ,Solomon SL

更新日期：1985-07-01 00:00:00

abstract：:A screening assay for phospholipase C using a chromogenic substrate incorporated into agar medium is described. The assay directly visualizes phospholipase C activity of mycoplasma lysates and membranes on agar plates, or the activity may be measured by spectrophotometry. The results from the assay confirm the presenc...

journal_title：Journal of clinical microbiology

authors： De Silva NS,Quinn PA

更新日期：1987-04-01 00:00:00

abstract：:Sputum obtained from patients with cystic fibrosis (CF) is highly viscous and often heterogeneous in bacterial distribution. Adding dithiothreitol (DTT) is the standard method for liquefaction prior to processing sputum for molecular detection assays. To determine if DTT treatment homogenizes the bacterial distributio...

journal_title：Journal of clinical microbiology

authors： Stokell JR,Khan A,Steck TR

更新日期：2014-07-01 00:00:00

abstract：:We document for the first time an infectious but nonarthritogenic variant of Borrelia burgdorferi. Strain 25015, previously isolated from an Ixodes dammini larva collected in upstate New York, was infectious but failed to produce arthritis or carditis in laboratory rats and mice. By contrast, pathogenic strain N40 inv...

journal_title：Journal of clinical microbiology

authors： Anderson JF,Barthold SW,Magnarelli LA

更新日期：1990-12-01 00:00:00

abstract：:Rapid tests for glucose, maltose, lactose, sucrose, and starch fermentation were performed on 112 strains of anaerobic bacteria. The tests were incubated under aerobic conditions, and results were read within 4 h. An overall correlation of 89% was achieved between the rapid tests and the Virginia Polytechnic Institut...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,Blazevic DJ

更新日期：1976-03-01 00:00:00

abstract：:A passive hemagglutination (PHA) assay for antibody to Pseudomonas aeruginosa exotoxin is described which utilizes chromic chloride-treated ovine erythrocytes coated with purified toxin. PHA antitoxin titers correlated well with those obtained by a cytotoxicity neutralization assay (r = 0.91, P less than 0.001), where...

journal_title：Journal of clinical microbiology

authors： Pollack M,Taylor NS

更新日期：1977-07-01 00:00:00

abstract：:The Sensititre Breakpoint Autoreader system (SBAS) is a broth microdilution method with one to three concentrations of each antibiotic and innovative fluorescence technology to define inhibitory endpoints. We tested 248 gram-negative bacilli and 80 gram-positive cocci using both the rapid (5 h) and overnight (18 h) SB...

journal_title：Journal of clinical microbiology

authors： Nolte FS,Krisher KK,Beltran LA,Christianson NP,Sheridan GE

更新日期：1988-06-01 00:00:00

abstract：:Comparative sequence analysis was performed upon Bacillus anthracis and its closest relatives, B. cereus and B. thuringiensis. Portions of rpoB DNA from 10 strains of B. anthracis, 16 of B. cereus, 10 of B. thuringiensis, 1 of B. mycoides, and 1 of B. megaterium were amplified and sequenced. The determined rpoB sequen...

journal_title：Journal of clinical microbiology

authors： Ko KS,Kim JM,Kim JW,Jung BY,Kim W,Kim IJ,Kook YH

更新日期：2003-07-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:A highly sensitive second-generation hepatitis C virus (HCV) core antigen assay has recently been developed. We compared viral disappearance and first-phase kinetics between commercially available core antigen (Ag) assays, Lumipulse Ortho HCV Ag (Lumipulse-Ag), and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV ...

journal_title：Journal of clinical microbiology

authors： Takahashi M,Saito H,Higashimoto M,Atsukawa K,Ishii H

更新日期：2005-01-01 00:00:00

abstract：:Tissue cultures established from brain tissues of six patients with various chronic, degenerative diseases did not show evidence of viral agents. Sucrose gradient analysis of radioactively labeled culture fluid from two patients failed to reveal the presence of any incomplete or defective virus. Extracellular fluids f...

journal_title：Journal of clinical microbiology

authors： Lennette ET

更新日期：1975-05-01 00:00:00

abstract：:Over a 12-month period, 43 children in eight different wards of our hospital (Hôpital Robert Debré) were infected or colonized with Klebsiella pneumoniae strains producing extended broad-spectrum beta-lactamases. The epidemiology of the outbreak was studied by a molecular approach including the determination of the be...

journal_title：Journal of clinical microbiology

authors： Bingen EH,Desjardins P,Arlet G,Bourgeois F,Mariani-Kurkdjian P,Lambert-Zechovsky NY,Denamur E,Philippon A,Elion J

更新日期：1993-02-01 00:00:00

abstract：:Gram stains remain the cornerstone of diagnostic testing in the microbiology laboratory for the guidance of empirical treatment prior to availability of culture results. Incorrectly interpreted Gram stains may adversely impact patient care, and yet there are no comprehensive studies that have evaluated the reliability...

journal_title：Journal of clinical microbiology

authors： Samuel LP,Balada-Llasat JM,Harrington A,Cavagnolo R

更新日期：2016-06-01 00:00:00

abstract：:In order to increase the application potential of the direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in human serum samples, we developed an antigen based on stained and freeze-dried Leishmania donovani promastigotes. We describe here the evaluation of the performance of the DAT based ...

journal_title：Journal of clinical microbiology

authors： Meredith SE,Kroon NC,Sondorp E,Seaman J,Goris MG,van Ingen CW,Oosting H,Schoone GJ,Terpstra WJ,Oskam L

更新日期：1995-07-01 00:00:00

abstract：:This study demonstrates that significant reproducibility problems can occur during routine use of the Abbott Laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae. These problems can go undetected by the quality control procedures outlined in the manufacturer's package insert. We outline here proc...

journal_title：Journal of clinical microbiology

authors： Gronowski AM,Copper S,Baorto D,Murray PR

更新日期：2000-06-01 00:00:00

abstract：:A new self-contained medium system for the cultivation of Neisseria gonorrhoeae was compared to modified Thayer-Martin medium for the diagnosis of gonorrhea from endocervical specimens. There was no difference in the ability of the two methods to support the growth of N. gonorrhoeae. ...

journal_title：Journal of clinical microbiology

authors： Beverly A,Bailey-Griffin JR,Schwebke JR

更新日期：2000-10-01 00:00:00

abstract：:Our results show that impairment of the gastrointestinal tracts in human immunodeficiency virus (HIV)-positive patients is present in the early phases of HIV disease. This impairment is associated with alterations in gut microbiota and intestinal inflammatory parameters. These findings support the hypothesis that alte...

journal_title：Journal of clinical microbiology

authors： Gori A,Tincati C,Rizzardini G,Torti C,Quirino T,Haarman M,Ben Amor K,van Schaik J,Vriesema A,Knol J,Marchetti G,Welling G,Clerici M

更新日期：2008-02-01 00:00:00

abstract：:Cerebrospinal fluid samples from 257 patients with suspected herpes simplex virus encephalitis were prospectively analyzed by herpes simplex virus polymerase chain reaction. The polymerase chain reaction indicated herpes simplex virus encephalitis in 9 serologically proven cases and in 14 additional patients. Increase...

journal_title：Journal of clinical microbiology

authors： Puchhammer-Stöckl E,Heinz FX,Kundi M,Popow-Kraupp T,Grimm G,Millner MM,Kunz C

更新日期：1993-01-01 00:00:00

abstract：:Bartonella henselae is the main causative agent of cat-scratch disease, and both B. henselae and Bartonella quintana cause angioproliferative disorders such as bacillary angiomatosis. To increase the sensitivity of Bartonella detection by PCR and to improve the species differentiation, we developed a semiquantitative,...

journal_title：Journal of clinical microbiology

authors： Sander A,Penno S

更新日期：1999-10-01 00:00:00

abstract：:The NCCLS M38-A document does not describe guidelines for testing caspofungin acetate (MK-0991) and other echinocandins against molds. This study evaluated the susceptibilities of 200 isolates of Aspergillus fumigatus, A. flavus, A. nidulans, A. niger, and A. terreus to caspofungin (MICs and minimum effective concentr...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A

更新日期：2003-01-01 00:00:00

abstract：:During lyssavirus surveillance, 1,221 bats of at least 30 species were collected from 25 locations in Kenya. One isolate of Lagos bat virus (LBV) was obtained from a dead Eidolon helvum fruit bat. The virus was most similar phylogenetically to LBV isolates from Senegal (1985) and from France (imported from Togo or Egy...

journal_title：Journal of clinical microbiology

authors： Kuzmin IV,Niezgoda M,Franka R,Agwanda B,Markotter W,Beagley JC,Urazova OY,Breiman RF,Rupprecht CE

更新日期：2008-04-01 00:00:00

abstract：:Clostridium perfringens is an important pathogen of animals and humans and is the causative agent of necrotic enteritis (NE) in poultry. This study focuses on the typing of intestinal C. perfringens isolates (n = 61) from outbreaks of NE collected from several areas of Southern Ontario, using a recently developed mult...

journal_title：Journal of clinical microbiology

authors： Chalmers G,Bruce HL,Hunter DB,Parreira VR,Kulkarni RR,Jiang YF,Prescott JF,Boerlin P

更新日期：2008-12-01 00:00:00

abstract：:We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs wa...

journal_title：Journal of clinical microbiology

authors： Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

更新日期：2015-11-01 00:00:00

abstract：:We demonstrate that all nine species of the Burkholderia cepacia complex can express the mucoid phenotype. A survey of clinical isolates showed that strains of B. cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can conve...

journal_title：Journal of clinical microbiology

authors： Zlosnik JE,Hird TJ,Fraenkel MC,Moreira LM,Henry DA,Speert DP

更新日期：2008-04-01 00:00:00

abstract：:We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species le...

journal_title：Journal of clinical microbiology

authors： Won H,Rothman R,Ramachandran P,Hsieh YH,Kecojevic A,Carroll KC,Aird D,Gaydos C,Yang S

更新日期：2010-09-01 00:00:00

abstract：:Spirochetes of the genus Treponema were cultured from 7 of 10 cases of digital dermatitis in sheep. Two cultures comprised Treponema phagedenis-like and Treponema medium/Treponema vincentii-like spirochetes, respectively, while the remaining cultures comprised mixed populations of Treponema medium/Treponema vincentii-...

journal_title：Journal of clinical microbiology

authors： Sayers G,Marques PX,Evans NJ,O'Grady L,Doherty ML,Carter SD,Nally JE

更新日期：2009-04-01 00:00:00