Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens.

abstract：:Three susceptibility testing procedures were compared to determine fluconazole, itraconazole, and ketoconazole MICs against 47 Candida albicans strains isolated sequentially from the oral cavities of five AIDS patients undergoing azole therapy. They included the broth microdilution method (BM), performed according to ...

journal_title：Journal of clinical microbiology

authors： Tortorano AM,Viviani MA,Barchiesi F,Arzeni D,Rigoni AL,Cogliati M,Compagnucci P,Scalise G

更新日期：1998-06-01 00:00:00

abstract：:We compared serial measurements of antibodies to mannan and to a cytoplasmic antigen (enzyme-linked immunosorbent assays), detection of mannan and an unidentified candidal antigen (latex agglutination), and assays of mannose and arabinitol (gas chromatographic assay of per-O-acetylated aldonitrile derivatives). In a h...

journal_title：Journal of clinical microbiology

authors： Greenfield RA,Troutt DL,Rickard RC,Altmiller DH

更新日期：1988-03-01 00:00:00

abstract：:Specific antibodies against human immunodeficiency virus (HIV), usually used for diagnosis, almost invariably become detectable within 3 months of exposure. We report on a patient whose HIV infection was identified early by a combined antigen/antibody test, but seroconversion did not occur for 7 months, until the impl...

journal_title：Journal of clinical microbiology

authors： Chin BS,Lee SH,Kim GJ,Kee MK,Suh SD,Kim SS

更新日期：2007-05-01 00:00:00

abstract：:Noroviruses are the leading cause of outbreaks of gastroenteritis in the world. At present, norovirus genogroup II, genotype 4 (GII/4), strains are the most prevalent in many countries. In this study we investigated 55 outbreaks and 35 sporadic cases of norovirus-associated gastroenteritis in food handlers in food-cat...

journal_title：Journal of clinical microbiology

authors： Ozawa K,Oka T,Takeda N,Hansman GS

更新日期：2007-12-01 00:00:00

abstract：:Standard repetitive extragenic palindromic (REP)-PCR, enterobacterial repetitive intergenic consensus-PCR, and Salmonella enteritidis repetitive element-PCR methods for bacterial strain typing were performed with DNA extracted by boiling members of each of the currently recognized species of human viridans group strep...

journal_title：Journal of clinical microbiology

authors： Alam S,Brailsford SR,Whiley RA,Beighton D

更新日期：1999-09-01 00:00:00

abstract：:Buruli ulcer (BU) is a necrotizing infection of skin and soft tissue caused by Mycobacterium ulcerans. In Australia, most cases of BU are linked to temperate, coastal Victoria and tropical, northern Queensland, and strains from these regions are distinguishable by variable-number tandem repeat (VNTR) typing. We presen...

journal_title：Journal of clinical microbiology

authors： Lavender CJ,Globan M,Johnson PD,Charles PG,Jenkin GA,Ghosh N,Clark BM,Martinello M,Fyfe JA

更新日期：2012-11-01 00:00:00

abstract：:The distribution of Chlamydia trachomatis serovars and Neisseria gonorrhoeae coinfection was studied in a group of 100 C. trachomatis-positive males with urethritis in Greece. The serovar distribution revealed that apart from the predominant worldwide types E and F, the relatively uncommon type G is also prevalent. Go...

journal_title：Journal of clinical microbiology

authors： Papadogeorgakis H,Pittaras TE,Papaparaskevas J,Pitiriga V,Katsambas A,Tsakris A

更新日期：2010-06-01 00:00:00

abstract：:We report the first case of spontaneous intrapartum Atopobium vaginae bacteremia identified by 16S rRNA gene sequencing. The bacterium was misidentified by RapID ANA II, API Rapid ID 32A, and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The likely source of bacteremia wa...

journal_title：Journal of clinical microbiology

authors： Chan JF,Lau SK,Curreem SO,To KK,Leung SS,Cheng VC,Yuen KY,Woo PC

更新日期：2012-07-01 00:00:00

abstract：:A novel PCR detection assay that amplifies the Helicobacter pylori-specific vacuolating cytotoxin gene (vacA) and thus enables rapid diagnosis of infection is described. Additionally, a real-time probe hybridization melting point analysis assay to detect all three mutations in the 23S rRNA gene associated with clarith...

journal_title：Journal of clinical microbiology

authors： Chisholm SA,Owen RJ,Teare EL,Saverymuttu S

更新日期：2001-04-01 00:00:00

abstract：:A simple enzyme-linked immunosorbent assay was developed for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii. Six monoclonal antibodies were used: two (F23-49 and F24-2) were specific for the M. tuberculosis co...

journal_title：Journal of clinical microbiology

authors： Schöningh R,Verstijnen CP,Kuijper S,Kolk AH

更新日期：1990-04-01 00:00:00

abstract：:The QuantiFERON-TB Gold Plus (QFT-Plus; Qiagen, Germantown, MD) interferon gamma release assay (IGRA) received FDA clearance in 2017 and will replace the prior version of the assay, the QFT-Gold In-Tube (QFT-GIT). Here, we compared performances of the QFT-Plus assay and the QFT-GIT version in a diverse patient populat...

journal_title：Journal of clinical microbiology

authors： Theel ES,Hilgart H,Breen-Lyles M,McCoy K,Flury R,Breeher LE,Wilson J,Sia IG,Whitaker JA,Clain J,Aksamit TR,Escalante P

更新日期：2018-06-25 00:00:00

abstract：:Serratia plymuthica was isolated from the blood and from a central venous catheter tip in a 54-year-old man with alcoholic cirrhosis and clinical signs of sepsis. This was the seventh reported isolate of S. plymuthica from a clinical specimen and the first in which the organism was clearly a significant pathogen. ...

journal_title：Journal of clinical microbiology

authors： Horowitz HW,Nadelman RB,Van Horn KG,Weekes SE,Goyburu L,Wormser GP

更新日期：1987-08-01 00:00:00

abstract：:In this report, we present a case of rhino-orbital mucormycosis in a 57-year-old female with poorly controlled diabetes mellitus. The only mold cultured at 25°C, 37°C, and 40°C from a specimen of the nasal crust was identified phenotypically and independently using nuclear ribosomal DNA sequence data as Thamnostylum l...

journal_title：Journal of clinical microbiology

authors： Xess I,Mohapatra S,Shivaprakash MR,Chakrabarti A,Benny GL,O'Donnell K,Padhye AA

更新日期：2012-04-01 00:00:00

abstract：:Anthopsis deltoidea was found to be the cause of an olecranon bursitis in a 79-year-old golfer. Serial histological sections of the olecranon bursa showed faintly stained, brown-walled, septate, hyphal elements in the centers of the necrotic debris. The combination of bursectomy and flucytosine treatment cured the inf...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Droller DD

更新日期：1984-08-01 00:00:00

abstract：:Standardization of sequence chromatogram analysis is required for consistent genotypic tropism determination across laboratories. A freely available, fast, and automated chromatogram analysis tool (RECall) provided tropism interpretations equivalent to those of manual sequence editing of 521 V3 loop HIV-1 population s...

journal_title：Journal of clinical microbiology

authors： Pou C,Bellido R,Casadellà M,Puig T,Clotet B,Harrigan R,Paredes R

更新日期：2013-08-01 00:00:00

abstract：:Single multiplexed assays could replace the standard 2-tiered (STT) algorithm recommended for the laboratory diagnosis of Lyme disease if they perform with a specificity and a sensitivity superior or equal to those of the STT algorithm. We used human serum rigorously characterized to be sera from patients with acute- ...

journal_title：Journal of clinical microbiology

authors： Arumugam S,Nayak S,Williams T,di Santa Maria FS,Guedes MS,Chaves RC,Linder V,Marques AR,Horn EJ,Wong SJ,Sia SK,Gomes-Solecki M

更新日期：2019-11-22 00:00:00

abstract：:Infectious gastroenteritis is one of the most common diseases in young children. To clarify the infectious etiology of diarrhea in Danish children less than 5 years of age, we conducted a 2-year prospective case-control study. Stools from 424 children with diarrhea and 870 asymptomatic age-matched controls were examin...

journal_title：Journal of clinical microbiology

authors： Olesen B,Neimann J,Böttiger B,Ethelberg S,Schiellerup P,Jensen C,Helms M,Scheutz F,Olsen KE,Krogfelt K,Petersen E,Mølbak K,Gerner-Smidt P

更新日期：2005-08-01 00:00:00

abstract：:Hepatitis C virus (HCV) genotype 4 (HCV-4) infection is considered to be difficult to treat and has become increasingly prevalent in European countries, including The Netherlands. Using a molecular epidemiological approach, the present study investigates the genetic diversity and evolutionary origin of HCV-4 in Amster...

journal_title：Journal of clinical microbiology

authors： de Bruijne J,Schinkel J,Prins M,Koekkoek SM,Aronson SJ,van Ballegooijen MW,Reesink HW,Molenkamp R,van de Laar TJ

更新日期：2009-12-01 00:00:00

abstract：:Seven commercial immunochromatographic assays intended for the detection of group A rotavirus antigens in human stool samples were evaluated. These assays showed similar levels of diagnostic accuracy and were suitable for the detection of rotavirus in patients with acute gastroenteritis but missed some asymptomatic ro...

journal_title：Journal of clinical microbiology

authors： Kaplon J,Fremy C,Pillet S,Mendes Martins L,Ambert-Balay K,Aho SL,Pothier P

更新日期：2015-11-01 00:00:00

abstract：:A method for cultivating and isolating Lyme disease spirochetes, Borrelia burgdorferi, from the saliva of vector ticks, Ixodes scapularis (formerly known as Ixodes dammini), is described. Saliva was collected from partially engorged ticks after application of pilocarpine to induce salivation. B. burgdorferi was isolat...

journal_title：Journal of clinical microbiology

authors： Ewing C,Scorpio A,Nelson DR,Mather TN

更新日期：1994-03-01 00:00:00

abstract：:Fluorophore-assisted carbohydrate electrophoresis (FACE) is a straightforward, sensitive method for determining the presence and relative abundance of individual oligomannosyl residues in Candida mannoprotein, the major antigenic determinant located on the outer surface of the yeast cell wall. The single terminal alde...

journal_title：Journal of clinical microbiology

authors： Goins TL,Cutler JE

更新日期：2000-08-01 00:00:00

abstract：:Streptococcus mutans strains were isolated from cohorts of Brazilian nursery school children and genotyped by arbitrarily primed PCR and restriction fragment length polymorphism analysis. Of 24 children with two to five S. mutans isolates, 29% carried two or more genotypes. The presence of matching genotypes of S. mut...

journal_title：Journal of clinical microbiology

authors： Mattos-Graner RO,Li Y,Caufield PW,Duncan M,Smith DJ

更新日期：2001-06-01 00:00:00

abstract：:A case of zygomycosis caused by Apophysomyces elegans in a patient having 25% full-thickness burns is described. Amputation of the leg was necessary to control rapid tissue invasion. The fungus was isolated from soil in the burn environment. ...

journal_title：Journal of clinical microbiology

authors： Cooter RD,Lim IS,Ellis DH,Leitch IO

更新日期：1990-09-01 00:00:00

abstract：:Sixty rotavirus-positive stool specimens from children with diarrhea were classified into G and P genotypes. G typing was done by PCR and then by hybridization with G type-specific (G1 to G4) oligonucleotide probes, whereas nested PCR was performed for P typing. Thirty-nine samples could be classified into both G and ...

journal_title：Journal of clinical microbiology

authors： Husain M,Seth P,Dar L,Broor S

更新日期：1996-06-01 00:00:00

abstract：:A monoclonal antibody (MAb), designated CHA 437, was developed against herpes simplex virus (HSV). This MAb (isotype, immunoglobulin G2b K) reacted with HSV type 1 and HSV type 2. It showed no cross-reactivity with varicella-zoster virus, cytomegalovirus, or Epstein-Barr virus. Direct detection of HSV antigen in clini...

journal_title：Journal of clinical microbiology

authors： Pouletty P,Chomel JJ,Thouvenot D,Catalan F,Rabillon V,Kadouche J

更新日期：1987-05-01 00:00:00

abstract：:Toxoplasmic lymphadenopathy (TL) is the most common clinical manifestation of acute acquired toxoplasma infection in normal individuals. The diagnosis is established by serologic methods and lymph node biopsy. Recently, tests for avidity of toxoplasma immunoglobulin G (IgG) antibodies have been introduced to help disc...

journal_title：Journal of clinical microbiology

authors： Montoya JG,Huffman HB,Remington JS

更新日期：2004-10-01 00:00:00

abstract：:The Clinical and Laboratory Standards Institute (CLSI) has revised several breakpoints since 2010 for bacteria that grow aerobically. In 2019, these revisions include changes to the ciprofloxacin and levofloxacin breakpoints for the Enterobacteriaceae and Pseudomonas aeruginosa, daptomycin breakpoints for Enterococcus...

journal_title：Journal of clinical microbiology

authors： Humphries RM,Abbott AN,Hindler JA

更新日期：2019-05-24 00:00:00

abstract：:Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures were recovered and examined for LT production. Preservation of the cultures on Dorset Egg Medium at 4 degrees C and pre...

journal_title：Journal of clinical microbiology

authors： Yoh M,Narita I,Honda T,Miwatani T,Nishibuchi M

更新日期：1991-10-01 00:00:00

abstract：:While Corynebacterium ulcerans can mimic classical diphtheria, extrapharyngeal infections are extremely rare. Sequencing of the diphtheria toxin (DT)-encoding tox gene of two C. ulcerans isolates from extrapharyngeal infections revealed differences from C. diphtheriae DT sequences, mainly in the translocation and rece...

journal_title：Journal of clinical microbiology

authors： Sing A,Hogardt M,Bierschenk S,Heesemann J

更新日期：2003-10-01 00:00:00

abstract：:On 24 August 2020, the Centers for Disease Control and Prevention (CDC) updated its website to highlight that asymptomatic individuals, even those with exposure to a COVID-19-positive contact, do not necessarily need to be tested unless they have medical conditions associated with increased risk of severe illness from...

journal_title：Journal of clinical microbiology

authors： Schuetz AN,Hemarajata P,Mehta N,Campbell S,Mitchell S,Palavecino E,Butler-Wu S,Miller MB

更新日期：2020-12-17 00:00:00