Evaluation of the immunoglobulin G avidity test for diagnosis of toxoplasmic lymphadenopathy.

abstract：:Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were e...

journal_title：Journal of clinical microbiology

authors： Truant AL,Elliott SH,Kelly MT,Smith JH

更新日期：1981-05-01 00:00:00

abstract：:A surveillance network was implemented by the Istituto Superiore di Sanità of Rome in collaboration with laboratories of virology in Czech Republic, Slovenia, Croatia, Albania, and Bulgaria. About 1,500 rotavirus-positive stool samples were collected from children with severe gastroenteritis admitted to hospitals or o...

journal_title：Journal of clinical microbiology

authors： Tcheremenskaia O,Marucci G,De Petris S,Ruggeri FM,Dovecar D,Sternak SL,Matyasova I,Dhimolea MK,Mladenova Z,Fiore L,Rotavirus Study Group.

更新日期：2007-07-01 00:00:00

abstract：:Low-speed centrifugation-mediated adsorption was evaluated as an enhancement of infectivity of clinical and laboratory strains of cytomegalovirus (CMV) occurring with cells grown in conventional culture tubes. The time required for reporting of primary isolates of CMV from urine specimens adsorbed onto monolayers of W...

journal_title：Journal of clinical microbiology

authors： Oefinger PE,Shawar RM,Loo SH,Tsai LT,Arnett JK

更新日期：1990-05-01 00:00:00

abstract：:Chlamydia trachomatis was recoved from 20% (36/180) of women attending a venereal disease clinic. All infected women had chlamydial antibodies in their serum and cervical secretions. However, the background rates of chlamydial antibody in chlamydia-negative women were very high. Measurement of antibodies in serum (com...

journal_title：Journal of clinical microbiology

authors： Schachter J,Cles L,Ray R,Hines PA

更新日期：1979-11-01 00:00:00

abstract：:We are reporting on a nosocomial outbreak of 213 cases of vancomycin-resistant enterococcus infection involving 2,812 enterococcal isolates from patients over a period of 36 months. In 1990, the Enterococcus faecium vancomycin susceptibility rate was found to be 85.7% (36 of 42 cases), and an incidence of 10.9% (42 of...

journal_title：Journal of clinical microbiology

authors： Boyle JF,Soumakis SA,Rendo A,Herrington JA,Gianarkis DG,Thurberg BE,Painter BG

更新日期：1993-05-01 00:00:00

abstract：:Detection of Staphylococcus aureus isolates with intermediate vancomycin susceptibility (VISA) and heteroresistance (hVISA) remains problematic. The population analysis profile/area under the curve (PAP/AUC) is the gold standard but is cumbersome. We compared the performance of two Etest screening methods (macromethod...

journal_title：Journal of clinical microbiology

authors： Riederer K,Shemes S,Chase P,Musta A,Mar A,Khatib R

更新日期：2011-06-01 00:00:00

abstract：:Immunoglobulin M (IgM) and IgA antibodies against the major surface protein of Toxoplasma gondii were determined in a total of 195 human sera and five human cerebrospinal fluids by using a P30 membrane extract and the immunoblot technique. By using two different T. gondii strains (RH and BK) simultaneously as antigens...

journal_title：Journal of clinical microbiology

authors： Gross U,Roos T,Appoldt D,Heesemann J

更新日期：1992-06-01 00:00:00

abstract：:Candida parapsilosis, a pathogenic yeast, is composed of three newly designated genomic species that are physiologically and morphologically indistinguishable. Nosocomial infections caused by group I C. parapsilosis are often associated with the breakdown of infection control practices and the contamination of medical...

journal_title：Journal of clinical microbiology

authors： Lasker BA,Butler G,Lott TJ

更新日期：2006-03-01 00:00:00

abstract：:Disseminated Mycobacterium avium infection is a common complication in late-stage AIDS. We describe a patient seropositive for human immunodeficiency virus with a disseminated infection caused by mycobacteria. Acid-fast rods were visible by microscopy in stool and bone marrow and in specimens from skin, liver, spleen,...

journal_title：Journal of clinical microbiology

authors： Kirschner P,Vogel U,Hein R,Böttger EC

更新日期：1994-03-01 00:00:00

abstract：:Leishmania (Viannia) braziliensis causes three main types of American tegumentary leishmaniasis (ATL), localized cutaneous leishmaniasis (CL), mucosal leishmaniasis (ML), and disseminated leishmaniasis (DL). All forms are observed among individuals of Corte de Pedra, Brazil. We previously used random amplified markers...

journal_title：Journal of clinical microbiology

authors： Queiroz A,Sousa R,Heine C,Cardoso M,Guimarães LH,Machado PR,Carvalho EM,Riley LW,Wilson ME,Schriefer A

更新日期：2012-12-01 00:00:00

abstract：:A 24-year-old man sustained a crush injury to the tibia, which subsequently became infected with Saksenaea vasiformis. He was treated with debridement and a free myocutaneous flap, but amputation was necessary because of mycotic osteomyelitis. S. vasiformis was recovered and identified on the basis of its characterist...

journal_title：Journal of clinical microbiology

authors： Pierce PF,Wood MB,Roberts GD,Fitzgerald RH Jr,Robertson C,Edson RS

更新日期：1987-05-01 00:00:00

abstract：:We used the slide agglutination test to determine the serologic relationships of 22 Legionella spp. representing 33 serogroups. Antisera prepared against 14 of the Legionella spp. contained cross-reactive antibodies (1+ or greater) at their working dilutions. Numerous cross-reactions were observed for the blue-white f...

journal_title：Journal of clinical microbiology

authors： Thacker WL,Plikaytis BB,Wilkinson HW

更新日期：1985-05-01 00:00:00

abstract：:Restriction fragment length polymorphism (RFLP) analysis with probes derived from the insertion element IS6110, the direct repeat sequence, and the polymorphic GC-rich sequence (PGRS) and a PCR-based typing method called spacer oligonucleotide typing (spoligotyping) were used to strain type Mycobacterium bovis isolate...

journal_title：Journal of clinical microbiology

authors： Costello E,O'Grady D,Flynn O,O'Brien R,Rogers M,Quigley F,Egan J,Griffin J

更新日期：1999-10-01 00:00:00

abstract：:BBL CHROMagar VanRE (CVRE) was compared with bile esculin azide agar plus vancomycin to screen for vancomycin-resistant enterococcus (VRE) colonization. CVRE distinguishes Enterococcus faecalis (green colonies) from Enterococcus faecium (mauve colonies) on the basis of chromogenic substrate use. CVRE sensitivity and s...

journal_title：Journal of clinical microbiology

authors： Kallstrom G,Doern CD,Dunne WM Jr

更新日期：2010-03-01 00:00:00

abstract：:A novel PCR detection assay that amplifies the Helicobacter pylori-specific vacuolating cytotoxin gene (vacA) and thus enables rapid diagnosis of infection is described. Additionally, a real-time probe hybridization melting point analysis assay to detect all three mutations in the 23S rRNA gene associated with clarith...

journal_title：Journal of clinical microbiology

authors： Chisholm SA,Owen RJ,Teare EL,Saverymuttu S

更新日期：2001-04-01 00:00:00

abstract：:This study used a nested multiplex PCR method to detect three periodontal pathogens in subgingival plaque collected before treatment and at 2 and 6 months posttreatment from 107 patients with severe, generalized periodontitis. The proportions of the patients who harbored these bacteria before periodontal treatment wer...

journal_title：Journal of clinical microbiology

authors： Ready D,D'Aiuto F,Spratt DA,Suvan J,Tonetti MS,Wilson M

更新日期：2008-10-01 00:00:00

abstract：:In this study, the currently known typing methods for Mycobacterium tuberculosis isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 M. tuberculosis complex strains, originating from 38 countries, were tested in five restriction fragment length polymorphism (RFLP) typ...

journal_title：Journal of clinical microbiology

authors： Kremer K,van Soolingen D,Frothingham R,Haas WH,Hermans PW,Martín C,Palittapongarnpim P,Plikaytis BB,Riley LW,Yakrus MA,Musser JM,van Embden JD

更新日期：1999-08-01 00:00:00

abstract：:A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December 2004. The findings were suggestive of increased recent exposure in both tsunami survivors and uninjured bystanders. Survi...

journal_title：Journal of clinical microbiology

authors： Wuthiekanun V,Chierakul W,Rattanalertnavee J,Langa S,Sirodom D,Wattanawaitunechai C,Winothai W,White NJ,Day N,Peacock SJ

更新日期：2006-01-01 00:00:00

abstract：:To diagnose catheter-related sepsis without removing the catheter, we evaluated the differential positivity times of cultures of blood drawn simultaneously from central venous catheter and peripheral sites. In a 450-bed cancer reference center, simultaneous central- and peripheral-blood cultures were prospectively per...

journal_title：Journal of clinical microbiology

authors： Blot F,Schmidt E,Nitenberg G,Tancrède C,Leclercq B,Laplanche A,Andremont A

更新日期：1998-01-01 00:00:00

abstract：:During a taxonomic investigation of Nocardia spp. and Streptomyces spp., we received 658 isolates from laboratories of both human and veterinary medicine. Our procedure leading to the identification of 92% of these isolates, the species that they represented, and a characteristic pattern of properties of the strains o...

journal_title：Journal of clinical microbiology

authors： Mishra SK,Gordon RE,Barnett DA

更新日期：1980-06-01 00:00:00

abstract：:The microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16S rRNA genes were randomly amplified by means of conserved eubacterial primers and cloned. Restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16S rRNA from the cult...

journal_title：Journal of clinical microbiology

authors： Dymock D,Weightman AJ,Scully C,Wade WG

更新日期：1996-03-01 00:00:00

abstract：:It has been shown by analysis with monoclonal and polyclonal antibodies that outer surface protein C (OspC) of Borrelia burgdorferi sensu lato is highly heterogeneous. To determine if the heterogeneity has a genetic basis, the genes of 18 different B. burgdorferi sensu lato strains have been amplified by PCR, cloned, ...

journal_title：Journal of clinical microbiology

authors： Jauris-Heipke S,Liegl G,Preac-Mursic V,Rössler D,Schwab E,Soutschek E,Will G,Wilske B

更新日期：1995-07-01 00:00:00

abstract：:The AutoMicrobic system (AMS) is capable of identifying most Enterobacteriaceae within 8 h and many glucose-nonfermenting, gram-negative bacilli after 13 h of incubation. Early preliminary results can be readily obtained from the computer as the tests incubate. Data with 1,023 bacterial isolates were reviewed to deter...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE

更新日期：1982-08-01 00:00:00

abstract：:The development of a specimen collection and transport medium outfit for the rapid laboratory diagnosis of whoping cough is described. The transport medium consisted of a semisolid agar containing charcoal, cephalexin, and defibrinated horse blood. It was also found to be an excellent enrichment medium for the selecti...

journal_title：Journal of clinical microbiology

authors： Regan J,Lowe F

更新日期：1977-09-01 00:00:00

abstract：:A novel reverse primer (GLM MR1) was designed for detection of the glmM gene in Helicobacter pylori by PCR. The percentage of amplification in clinical isolates using GLM MR1 was 100% for detection of the glmM gene and 86.36% for the ureA gene. The primer designed is useful for the identification of H. pylori. ...

journal_title：Journal of clinical microbiology

authors： Espinoza MG,Vazquez RG,Mendez IM,Vargas CR,Cerezo SG

更新日期：2011-04-01 00:00:00

abstract：:Coagulase-negative staphylococci obtained from patients with continuous ambulatory peritoneal dialysis-related peritonitis were cultured in nutrient broth in an atmosphere of air containing 5% carbon dioxide (reflecting the CO2 tension found in freshly used dialysate). Significant differences were observed between the...

journal_title：Journal of clinical microbiology

authors： Denyer SP,Davies MC,Evans JA,Finch RG,Smith DG,Wilcox MH,Williams P

更新日期：1990-08-01 00:00:00

abstract：:Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization...

journal_title：Journal of clinical microbiology

authors： Kusunoki S,Ezaki T,Tamesada M,Hatanaka Y,Asano K,Hashimoto Y,Yabuuchi E

更新日期：1991-08-01 00:00:00

abstract：:Marburg virus (MARV) causes a severe hemorrhagic fever in humans with a high mortality rate. The rapid and accurate identification of the virus is required to appropriately provide infection control and outbreak management. Here, we developed and evaluated a one-step reverse transcription-loop-mediated isothermal ampl...

journal_title：Journal of clinical microbiology

authors： Kurosaki Y,Grolla A,Fukuma A,Feldmann H,Yasuda J

更新日期：2010-07-01 00:00:00

abstract：:Screening for Chlamydia trachomatis infection in men has traditionally been limited to men who present with urethral symptoms, thereby limiting the detection of asymptomatic chlamydia infection in men. In order to effectively screen both symptomatic and asymptomatic men, we evaluated a newly developed polymerase chain...

journal_title：Journal of clinical microbiology

authors： Jaschek G,Gaydos CA,Welsh LE,Quinn TC

更新日期：1993-05-01 00:00:00

abstract：:Production of staphylococcal alpha- or alpha-beta-toxins correlated well with production of coagulase or thermonuclease (or both) in 203 Staphylococcus aureus isolates from milk and should be reliable indicators of S. aureus in the absence of Staphylococcus intermedius. Failures to produce toxin, tube coagulase, or th...

journal_title：Journal of clinical microbiology

authors： Jasper DE,Infante F,Dellinger JD

更新日期：1985-04-01 00:00:00