Influence of carbon dioxide on the surface characteristics and adherence potential of coagulase-negative staphylococci.

abstract：:Droplet digital PCR (ddPCR) is an emulsion PCR process that performs absolute quantitation of nucleic acids. We developed a ddPCR assay for Chlamydia trachomatis infections and found it to be accurate and precise. Using PCR mixtures containing plasmids engineered to include the PCR target sequences, we were able to qu...

journal_title：Journal of clinical microbiology

authors： Roberts CH,Last A,Molina-Gonzalez S,Cassama E,Butcher R,Nabicassa M,McCarthy E,Burr SE,Mabey DC,Bailey RL,Holland MJ

更新日期：2013-07-01 00:00:00

abstract：:Penicilliosis, caused by the dimorphic fungus Penicillium marneffei, is an important opportunistic systemic fungal infection affecting immunocompromised individuals living in areas where penicilliosis is endemic. We have demonstrated previously that a urinary enzyme-linked immunosorbent assay (ELISA) with purified rab...

journal_title：Journal of clinical microbiology

authors： Desakorn V,Simpson AJ,Wuthiekanun V,Sahassananda D,Rajanuwong A,Pitisuttithum P,Howe PA,Smith MD,White NJ

更新日期：2002-09-01 00:00:00

abstract：:The performance of a commercially available, rapid membrane enzyme immunoassay for influenza A and B virus detection was compared to that of viral culture in 4,092 respiratory specimens collected from patients presenting with respiratory symptoms during the 2002-2003 influenza season. The test's overall sensitivity wa...

journal_title：Journal of clinical microbiology

authors： Cazacu AC,Chung SE,Greer J,Demmler GJ

更新日期：2004-08-01 00:00:00

abstract：:A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the res...

journal_title：Journal of clinical microbiology

authors： Liang SY,Chan YH,Hsia KT,Lee JL,Kuo MC,Hwa KY,Chan CW,Chiang TY,Chen JS,Wu FT,Ji DD

更新日期：2009-06-01 00:00:00

abstract：:A 1994 outbreak of acute hemorrhagic conjunctivitis in Israel was caused by an enterovirus 70 strain that was distinct from previously reported strains. Characterization was by electron microscopy (eye washes), reverse transcription-PCR (RT-PCR; eyewash, specimens, eye swabs, and tears), and sequence analysis of RT-PC...

journal_title：Journal of clinical microbiology

authors： Shulman LM,Manor Y,Azar R,Handsher R,Vonsover A,Mendelson E,Rothman S,Hassin D,Halmut T,Abramovitz B,Varsano N

更新日期：1997-08-01 00:00:00

abstract：:Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus in the family Bunyaviridae, genus Nairovirus. The virus is transmitted to humans through infected tick bites or from direct contact with viremic animals or humans. In the present study, a total of 1,015 adult ticks were collected from cattle (603 spec...

journal_title：Journal of clinical microbiology

authors： Tonbak S,Aktas M,Altay K,Azkur AK,Kalkan A,Bolat Y,Dumanli N,Ozdarendeli A

更新日期：2006-11-01 00:00:00

abstract：:In 1997 and 1998, H3N2 influenza A viruses emerged among pigs in North America. Genetic analyses of the H3N2 isolates demonstrated that they had distinctly different genotypes. The most commonly isolated viruses in the United States have a triple-reassortant genotype, with the hemagglutinin, neuraminidase, and PB1 pol...

journal_title：Journal of clinical microbiology

authors： Landolt GA,Karasin AI,Phillips L,Olsen CW

更新日期：2003-05-01 00:00:00

abstract：:A new self-contained medium system for the cultivation of Neisseria gonorrhoeae was compared to modified Thayer-Martin medium for the diagnosis of gonorrhea from endocervical specimens. There was no difference in the ability of the two methods to support the growth of N. gonorrhoeae. ...

journal_title：Journal of clinical microbiology

authors： Beverly A,Bailey-Griffin JR,Schwebke JR

更新日期：2000-10-01 00:00:00

abstract：:Chlamydia pneumoniae has proved to be difficult to isolate and propagate in cell culture. We compared the growth of three strains of C. pneumoniae, TW-183 and two clinical isolates from Brooklyn, N.Y., in five cell lines, including HeLa 229, McCoy, HL, HEp-2, and HTED, an immortalized human tracheal cell line. HEp-2 w...

journal_title：Journal of clinical microbiology

authors： Roblin PM,Dumornay W,Hammerschlag MR

更新日期：1992-08-01 00:00:00

abstract：:With use of the rabbit intestinal loop model, heat-labile enterotoxins from 21 Escherichia coli strains isolated from a wide spectrum of patients with diarrheal diseases were all neutralized to high titer by two antisera prepared against enterotoxins of either E. coli or Vibrio cholerae. These findings suggest marked ...

journal_title：Journal of clinical microbiology

authors： Sack RB,Froehlich JL

更新日期：1977-06-01 00:00:00

abstract：:A case of zygomycosis caused by Apophysomyces elegans in a patient having 25% full-thickness burns is described. Amputation of the leg was necessary to control rapid tissue invasion. The fungus was isolated from soil in the burn environment. ...

journal_title：Journal of clinical microbiology

authors： Cooter RD,Lim IS,Ellis DH,Leitch IO

更新日期：1990-09-01 00:00:00

abstract：:A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a speci...

journal_title：Journal of clinical microbiology

authors： Sullivan TJ,Patel P,Hutchinson A,Ethridge SF,Parker MM

更新日期：2011-10-01 00:00:00

abstract：:We serotyped 222 Pasteurella multocida strains isolated from swine lungs at slaughter. Capsular serotypes A and D were determined by the hyaluronidase sensitivity and acriflavin agglutination tests, respectively. Somatic antigens were determined by gel diffusion against standard antisera. Capsular serotype A was found...

journal_title：Journal of clinical microbiology

authors： Pijoan C,Morrison RB,Hilley HD

更新日期：1983-06-01 00:00:00

abstract：:A broad range of viral and bacterial pathogens can cause acute respiratory tract infection. For rapid detection of a broad respiratory pathogen spectrum, multiplex real-time PCR is ideal. This study evaluated the performance of the new Luminex NxTAG Respiratory Pathogen Panel (NxTAG-RPP) in comparison with the BioFire...

journal_title：Journal of clinical microbiology

authors： Chen JHK,Lam HY,Yip CCY,Wong SCY,Chan JFW,Ma ESK,Cheng VCC,Tang BSF,Yuen KY

更新日期：2016-07-01 00:00:00

abstract：:A real-time PCR assay was developed targeting the bla(KPC) responsible for Klebsiella pneumoniae carbapenemase (KPC)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. The assay accurately detects KPC-containing strains with high analytical specificity and sensitivity. ...

journal_title：Journal of clinical microbiology

authors： Mangold KA,Santiano K,Broekman R,Krafft CA,Voss B,Wang V,Hacek DM,Usacheva EA,Thomson RB Jr,Kaul KL,Peterson LR

更新日期：2011-09-01 00:00:00

abstract：:A total of 28 unrelated isolates of the Salmonella enterica subsp. enterica serovar dublin (S. dublin) collected during a 6-year period, as well as four samples of the S. dublin live vaccine strain Bovisaloral and its prototype strain S. dublin 442/039, were investigated by different molecular typing methods for the f...

journal_title：Journal of clinical microbiology

authors： Liebisch B,Schwarz S

更新日期：1996-03-01 00:00:00

abstract：:The COVID-19 pandemic has necessitated a multifaceted rapid response by the scientific community, bringing researchers, health officials, and industry together to address the ongoing public health emergency. To meet this challenge, participants need an informed approach for working safely with the etiological agent, t...

journal_title：Journal of clinical microbiology

authors： Welch SR,Davies KA,Buczkowski H,Hettiarachchi N,Green N,Arnold U,Jones M,Hannah MJ,Evans R,Burton C,Burton JE,Guiver M,Cane PA,Woodford N,Bruce CB,Roberts ADG,Killip MJ

更新日期：2020-10-21 00:00:00

abstract：:A replicator method for screening Staphylococcus aureus isolates for synergistic hemolytic activity is described. The technique should also be useful for assessing the hemolytic activity of staphylococci and streptococci on different lots or brands of blood agar because the inoculum and the distances between spot inoc...

journal_title：Journal of clinical microbiology

authors： Boyce JM

更新日期：1985-05-01 00:00:00

abstract：:Urinary tract infections caused by staphylococci are usually attributed to Staphylococcus epidermidis or S. saprophyticus. The case study reported here describes a persistent urinary tract infection caused by S. haemolyticus in a 38-year-old male whose infection was ultimately resolved through the use of the antibioti...

journal_title：Journal of clinical microbiology

authors： Gunn BA,Davis CE Jr

更新日期：1988-05-01 00:00:00

abstract：:Multidrug-resistant tuberculous meningitis is fatal without rapid diagnosis and use of second-line therapy. It is more common in human immunodeficiency virus (HIV)-positive patients. Beijing genotype strains of Mycobacterium tuberculosis are associated with drug resistance, particularly multidrug resistance, and their...

journal_title：Journal of clinical microbiology

authors： Caws M,Thwaites G,Stepniewska K,Nguyen TN,Nguyen TH,Nguyen TP,Mai NT,Phan MD,Tran HL,Tran TH,van Soolingen D,Kremer K,Nguyen VV,Nguyen TC,Farrar J

更新日期：2006-11-01 00:00:00

abstract：:To determine the pandemic potential of Vibrio cholerae, one must demonstrate both the presence of O1 antigen and the production of enterotoxin (CT). Tissue culture or enzyme-linked immunosorbent assays (ELISAs) for CT have been limited to research and reference laboratories. A kit for detecting CT by reversed passive ...

journal_title：Journal of clinical microbiology

authors： Almeida RJ,Hickman-Brenner FW,Sowers EG,Puhr ND,Farmer JJ 3rd,Wachsmuth IK

更新日期：1990-01-01 00:00:00

abstract：:The first case of osteomyelitis caused by Neosartorya pseudofischeri is reported. The patient, a 77-year-old male with a history of silicosis and tuberculosis, on X-ray examination revealed lytic lesions of L2 and L3 vertebrae suspicious for metastatic lesions. Histologic examination of biopsy specimens from vertebral...

journal_title：Journal of clinical microbiology

authors： Padhye AA,Godfrey JH,Chandler FW,Peterson SW

更新日期：1994-11-01 00:00:00

abstract：:A variety of fungi produce the hydrolytic enzyme beta-N-acetylhexosaminidase (HexNAcase), which can be readily detected in assays by using p-nitrophenyl-N-acetyl-beta-D-glucosaminide as a substrate. In the present study we developed a microtiter plate-based HexNAcase assay for distinguishing Candida albicans and Candi...

journal_title：Journal of clinical microbiology

authors： Niimi K,Shepherd MG,Cannon RD

更新日期：2001-06-01 00:00:00

abstract：:Thirteen representative pathogenic bacterial species were used to create septicemia in rabbits, by injecting 10(6) colony-forming units into the marginal ear vein. At a selected time, usually 30 to 60 min after injection, heart blood was drawn into heparin and dispensed in 5.0-,0.5-, and 0.1-ml volumes into duplicate ...

journal_title：Journal of clinical microbiology

authors： Zierdt CH,Peterson DL,Swan JC,MacLowry JD

更新日期：1982-01-01 00:00:00

abstract：:We describe a novel, rapid, and inexpensive method for the measurement of growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium smegmatis in the presence or absence of detergent. The method, which employs hot NaOH treatment of mycobacterial cells to release total cellular protein, compares favor...

journal_title：Journal of clinical microbiology

authors： Meyers PR,Bourn WR,Steyn LM,van Helden PD,Beyers AD,Brown GD

更新日期：1998-09-01 00:00:00

abstract：:The control of vancomycin-resistant enterococci (VRE) has become an increasing burden on health care resources since their discovery over 20 years ago. Current techniques employed for their detection include time-consuming and laborious phenotypic methods or molecular methods requiring costly equipment and consumables...

journal_title：Journal of clinical microbiology

authors： Griffin PM,Price GR,Schooneveldt JM,Schlebusch S,Tilse MH,Urbanski T,Hamilton B,Venter D

更新日期：2012-09-01 00:00:00

abstract：:Saksenaea is a monotypic genus belonging to the order Mucorales and capable of producing severe human infections. Through a polyphasic study based on analysis of the sequences of the internal transcribed spacer (ITS) region, domains D1 and D2 of the 28S rRNA gene, and the elongation factor 1α (EF-1α) gene, as well as ...

journal_title：Journal of clinical microbiology

authors： Alvarez E,Garcia-Hermoso D,Sutton DA,Cano JF,Stchigel AM,Hoinard D,Fothergill AW,Rinaldi MG,Dromer F,Guarro J

更新日期：2010-12-01 00:00:00

abstract：:Two types of commercially available microdilution trays were inoculated with log-phase cultures, stationary-phase cultures, and direct suspensions of 18- to 24-h colonies, with essentially comparable results. Direct pick-up of colonies was also tested with two Prompt inoculation systems (3M Co.); they were found to be...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE,Hawkinson RW

更新日期：1983-09-01 00:00:00

abstract：:The human herpesvirus 6 (HHV-6) early protein, p41, encoded by the U27 gene has been detected in oligodendrocytes of multiple sclerosis (MS) patients by using a monoclonal antibody (MAb to p41/38). We here report the antigenic epitope of HHV-6 p41 recognized by this MAb. First, we established that the MAb to p41/38 re...

journal_title：Journal of clinical microbiology

authors： Xu Y,Linde A,Dahl H,Winberg G

更新日期：2001-04-01 00:00:00

abstract：:Automated treponemal immunoassays are used for syphilis screening with the reverse-sequence algorithm; discordant results (e.g., enzyme immunoassay [EIA] reactive and reactive plasma reagin [RPR] nonreactive) are resolved with a second treponemal test. We conducted a study to determine automated immunoassay signal str...

journal_title：Journal of clinical microbiology

authors： Fakile YF,Jost H,Hoover KW,Gustafson KJ,Novak-Weekley SM,Schapiro JM,Tran A,Chow JM,Park IU

更新日期：2017-12-26 00:00:00