Development of loop-mediated isothermal amplification assay for detection of Entamoeba histolytica.

abstract：:Plasmids encoding extended-spectrum beta-lactamases of the TEM, SHV, and AmpC families were introduced into common Escherichia coli and Klebsiella pneumoniae hosts to create a homogeneous panel for evaluating the abilities of five test systems to detect resistance to eight beta-lactam antibiotics. Although MICs, as de...

journal_title：Journal of clinical microbiology

authors： Katsanis GP,Spargo J,Ferraro MJ,Sutton L,Jacoby GA

更新日期：1994-03-01 00:00:00

abstract：:Two strains of a previously undescribed Actinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. Analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct fr...

journal_title：Journal of clinical microbiology

authors： Collins MD,Hoyles L,Kalfas S,Sundquist G,Monsen T,Nikolaitchouk N,Falsen E

更新日期：2000-09-01 00:00:00

abstract：:In clinical laboratories, diagnosis of imported malaria is commonly performed by microscopy. However, the volume of specimens is generally low and maintaining proficiency in reading blood smears, particularly at the species level, is challenging in this setting. To address this problem, the Provincial Laboratory for P...

journal_title：Journal of clinical microbiology

authors： Shokoples S,Mukhi SN,Scott AN,Yanow SK

更新日期：2013-06-01 00:00:00

abstract：:One of the capabilities developed by bacteria is the ability to gain large fragments of DNA from other bacteria or to lose portions of their own genomes. Among these exchangeable fragments are the genomic islands (GIs). Nine GIs have been identified in Brucella, and genomic island 3 (GI-3) is shared by two pathogenic ...

journal_title：Journal of clinical microbiology

authors： Céspedes S,Salgado P,Valenzuela P,Vidal R,Oñate AA

更新日期：2011-07-01 00:00:00

abstract：:The prevalence of the Epstein-Barr virus (EBV) Taiwan variant was investigated in the throat washing (TW) samples from patients with head and neck tumors, persons with nonmalignant diseases, and healthy adults in Taiwan. By using the EBV (BNLF-1 gene)-specific primers and PCR, the EBV latent membrane protein gene BNLF...

journal_title：Journal of clinical microbiology

authors： Jeng KC,Hsu CY,Liu MT,Chung TT,Liu ST

更新日期：1994-01-01 00:00:00

abstract：:Sera from patients with adult T-cell leukemia and asymptomatic carriers of human T-cell lymphotropic virus type I (HTLV-I) from widely separated areas of the world reacted strongly in a standardized quantitative enzyme-linked immunosorbent assay procedure with HTLV-I viral antigen prepared from a strain isolated in th...

journal_title：Journal of clinical microbiology

authors： Halbert SP,Poiesz B,Friedman-Kien AE,Montagna R,Blattner WA,Anken M

更新日期：1986-02-01 00:00:00

abstract：:Sera from patients with paracoccidioidomycosis (PCM), histoplasmosis (HP), or Jorge Lobo's disease (JL) were titrated against purified gp43 from Paracoccidioides brasiliensis by using both enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation (IPP) reactions with 125I-labeled antigens. In IPP, PCM sera and...

journal_title：Journal of clinical microbiology

authors： Puccia R,Travassos LR

更新日期：1991-08-01 00:00:00

abstract：:Cytomegalovirus (CMV) DNA loads in paired leukocyte and plasma samples from 199 patient visits by 66 patients with CMV retinitis were determined. Leukocyte CMV load determinations had a greater range of values (mean, 24,587 copies/10(6) leukocytes; maximum, 539, 000) than did plasma CMV load determinations (mean, 10,3...

journal_title：Journal of clinical microbiology

authors： Jabs DA,Forman M,Enger C,Jackson JB

更新日期：1999-05-01 00:00:00

abstract：:Fresh urine pellets from human immunodeficiency virus type 1 (HIV-1)-seropositive individuals were examined for the presence of the HIV-1 genomic sequence and gene products. By using the polymerase chain reaction technique, HIV-1 DNA proviral sequences were detected in 53 of 80 (66.25%) fresh urine pellets from HIV-1-...

journal_title：Journal of clinical microbiology

authors： Li JJ,Huang YQ,Poiesz BJ,Zaumetzger-Abbot L,Friedman-Kien AE

更新日期：1992-05-01 00:00:00

abstract：:Fluorescence of sensitized bacterial suspensions, reactive with either fluorescein-labeled or rhodamine-labeled antiglobulins, could be quantitatively distinguished in dual-labeled preparations by fluorescence immunoassay. ...

journal_title：Journal of clinical microbiology

authors： Gillis TP,Thompson JJ

更新日期：1978-09-01 00:00:00

abstract：:Stable dwarf forms of Staphylococcus aureus have been identified in clinical specimens as the sole or predominant isolate in eight cases. These organisms have been shown to be menadione or thiamine dependent, i.e., cultivation in the presence of one of these agents has permitted growth of colonies which appear typical...

journal_title：Journal of clinical microbiology

authors： Acar JF,Goldstein FW,Lagrange P

更新日期：1978-08-01 00:00:00

abstract：:Bartonella henselae or Bartonella elizabethae DNA from EDTA-anticoagulated blood samples obtained from four dogs was amplified and sequenced. The results showed that B. elizabethae should be added to the list of Bartonella species (i.e., B. vinsonii subsp. berkhoffii, B. henselae, and B. clarridgeiae) that are current...

journal_title：Journal of clinical microbiology

authors： Mexas AM,Hancock SI,Breitschwerdt EB

更新日期：2002-12-01 00:00:00

abstract：:We retrospectively analyzed epidemiologic information associated with 22 cultures of Neisseria elongata subsp. nitroreducens (formerly CDC group M-6) submitted to the Microbial Diseases Laboratory, California Department of Health Services, Berkeley, over a 16-year period. The most common illnesses noted with this bact...

journal_title：Journal of clinical microbiology

authors： Wong JD,Janda JM

更新日期：1992-03-01 00:00:00

abstract：:Typing of Mycobacterium avium subspecies paratuberculosis strains presents a challenge, since they are genetically monomorphic and traditional molecular techniques have limited discriminatory power. The recent advances and availability of whole-genome sequencing have extended possibilities for the characterization of ...

journal_title：Journal of clinical microbiology

authors： Leão C,Goldstone RJ,Bryant J,McLuckie J,Inácio J,Smith DG,Stevenson K

更新日期：2016-03-01 00:00:00

abstract：:A comparison of three different commercial media was made to assess their recovery of anaerobic organisms from the blood stream. The three media used were the 50-ml brain heart infusion broth with added CO2 (Pfizer), the 50-ml Thiol broth with added CO2 (Difco), and the 50-ml prereduced, supplemented peptone broth in ...

journal_title：Journal of clinical microbiology

authors： Mangels JI,Lindberg LH,Vosti KL

更新日期：1977-05-01 00:00:00

abstract：:Three cases of neonatal infection caused by Enterobacter sakazakii are reported from the Department of Neonatal Intensive Care, the National University Hospital, Reykjavík, Iceland. These infections occurred during a 9-month period in 1986 and 1987. Two of the neonates, who were normal at birth, survived but were left...

journal_title：Journal of clinical microbiology

authors： Biering G,Karlsson S,Clark NC,Jónsdóttir KE,Lúdvígsson P,Steingrímsson O

更新日期：1989-09-01 00:00:00

abstract：:A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency >/=5 x 10(-7) was established at >/=70 and >/=20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 microl of an...

journal_title：Journal of clinical microbiology

authors： Galán JC,Tato M,Baquero MR,Turrientes C,Baquero F,Martinez JL

更新日期：2004-09-01 00:00:00

abstract：:We compared a direct fluorescent-antibody stain (DFA) and an enzyme immunoassay (EIA) with a standard cell culture technique for the detection of Chlamydia trachomatis infection in women in an urban family practice setting. We also evaluated a DFA sample in a commercial laboratory to determine the interlaboratory reli...

journal_title：Journal of clinical microbiology

authors： Gann PH,Herrmann JE,Candib L,Hudson RW

更新日期：1990-07-01 00:00:00

abstract：:We report on the performance of a recently introduced commercial chessboard method using 12 antisera, in comparison with that of the 55-antiserum panel used in determining the serogroups and types (SGTs) of Streptococcus pneumoniae, both of which were carried out by a coagglutination technique. Of a total of 150 strai...

journal_title：Journal of clinical microbiology

authors： Lalitha MK,Thomas K,Kumar RS,Steinhoff MC

更新日期：1999-01-01 00:00:00

abstract：:Yersinia pseudotuberculosis produces novel superantigenic toxins designated YPMa (Y. pseudotuberculosis-derived mitogen), YPMb, and YPMc and has a pathogenicity island termed HPI (high-pathogenicity island) and R-HPI (the right-hand part of the HPI with truncation in its left-hand part) on the chromosome. Analysis of ...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Matsuda Y,Seki R,Tsubokura M,Takeda N,Shubin FN,Paik IK,Zheng XB

更新日期：2001-10-01 00:00:00

abstract：:A clinical evaluation was conducted in six North American centers to determine the ability of APTIMA CT (ACT) and APTIMA GC (AGC) nucleic acid amplification assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections in 1,322 men by testing their urethral swabs and first-catch urine (FCU). The results ...

journal_title：Journal of clinical microbiology

authors： Chernesky MA,Martin DH,Hook EW,Willis D,Jordan J,Wang S,Lane JR,Fuller D,Schachter J

更新日期：2005-01-01 00:00:00

abstract：:The comparison of Gram-stained urethral smears with Gen-Probe for the detection of Neisseria Gonorrhoeae in the urethras of males with symptomatic urethritis revealed a 99.6% correlation between the two methods. A simple Gram stain would appear to be the method of choice for the detection of gonorrhea in symptomatic m...

journal_title：Journal of clinical microbiology

authors： Juchau SV,Nackman R,Ruppart D

更新日期：1995-11-01 00:00:00

abstract：:A rapid method for presumptive identification of black-pigmented gram-negative anaerobic rods was developed. Using filter paper spot tests for indole production, sialidase, alpha-glucosidase, beta-glucosidase, alpha-fucosidase, and trypsinlike enzyme activities, 100% of Porphyromonas gingivalis, Prevotella intermedia,...

journal_title：Journal of clinical microbiology

authors： Moncla BJ,Braham P,Rabe LK,Hillier SL

更新日期：1991-09-01 00:00:00

abstract：:The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tåstrup, Denmark) and the Staph ID 32 API system (API System, BioMérieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated....

journal_title：Journal of clinical microbiology

authors： Renneberg J,Rieneck K,Gutschik E

更新日期：1995-05-01 00:00:00

abstract：:The performance of four media for the isolation of Aeromonas strains from stool specimens, the importance of ampicillin-susceptible Aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for Aeromonas strains were evaluated in two phases. In the fi...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Stroh EM,Jessop J

更新日期：1988-09-01 00:00:00

abstract：:Borrelia burgdorferi expresses a conserved, species-specific 39-kDa protein (P39) that can stimulate antibodies during human infection. To confirm that anti-P39 antibodies are produced consistently in animals exposed to infectious spirochetes, white-footed mice, Peromyscus leucopus, and laboratory white mice, Mus musc...

journal_title：Journal of clinical microbiology

authors： Simpson WJ,Burgdorfer W,Schrumpf ME,Karstens RH,Schwan TG

更新日期：1991-02-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response. Variability of the gG-1 gene among wild-type strains may be a factor of importance for a reliable serodiagnosis and typing of HSV-1 isolates. Here, we used a gG-1 type-spec...

journal_title：Journal of clinical microbiology

authors： Rekabdar E,Tunbäck P,Liljeqvist JA,Lindh M,Bergström T

更新日期：2002-09-01 00:00:00

abstract：:Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunctio...

journal_title：Journal of clinical microbiology

authors： Dupuis M,Brunt S,Appler K,Davis A,Rudd R

更新日期：2015-09-01 00:00:00

abstract：:Feces and serum specimens were collected from three farms in Michigan on which approximately 50-lb (8- to 9-week-old) pigs experienced diarrhea just after placement into all-in-all-out finishing barns. The clinical signs (profuse watery diarrhea lasting about 2 weeks and no vomiting) were similar on all farms, and the...

journal_title：Journal of clinical microbiology

authors： Kim Y,Chang KO,Straw B,Saif LJ

更新日期：1999-05-01 00:00:00

abstract：:Three hundred ninety single sera from adults in Japan, Indonesia, Singapore, and Papua New Guinea were tested for antibodies to human calicivirus (HCV) by a radioimmunoassay blocking test. A high prevalence of antibodies was observed in samples collected in Japan and Southeast Asia. Of 240 serum specimens collected fr...

journal_title：Journal of clinical microbiology

authors： Nakata S,Chiba S,Terashima H,Nakao T

更新日期：1985-10-01 00:00:00