Serotyping of Streptococcus pneumoniae by coagglutination with 12 pooled antisera.

abstract：:A TaqMan PCR was established for identification and quantitation of members of the Ehrlichia phagocytophila group in experimentally infected cows and in Ixodes ricinus ticks. The TaqMan PCR identified a 106-bp section of the 16S rRNA gene by use of a specific fluorogenic probe and two primers. This technique was speci...

journal_title：Journal of clinical microbiology

authors： Pusterla N,Huder JB,Leutenegger CM,Braun U,Madigan JE,Lutz H

更新日期：1999-05-01 00:00:00

abstract：:Human adenovirus type 4 is one of the major serotypes isolated from patients with adenoviral conjunctivitis. In 2001 we encountered nosocomial infections with epidemic conjunctivitis in the ophthalmology ward of one hospital in Sapporo, which is in the northern part of Japan. Adenoviruses were isolated from the patien...

journal_title：Journal of clinical microbiology

authors： Ariga T,Shimada Y,Ohgami K,Tagawa Y,Ishiko H,Aoki K,Ohno S

更新日期：2004-08-01 00:00:00

abstract：:Commercially prepared fluorescein-labeled antihuman antibodies were labed with 125I and used to compre specific herpes simplex virus antibody titers as determined by indirect fluorescent antibody and radioimmunoassay techniques. Total virus-specific immunoglobulin and virus-specific immunoglobulin G titers did not var...

journal_title：Journal of clinical microbiology

authors： Parkinson AJ,Kalmakoff J

更新日期：1976-06-01 00:00:00

abstract：:Assessment of cure of Trypanosoma cruzi infection by antibody seroconversion usually involves several years of follow-up. Parasitological negativity is useless for cure assessment, since even untreated patients mostly show negative results; conversely, positive tests are of great value because they indicate treatment ...

journal_title：Journal of clinical microbiology

authors： Galvão LM,Chiari E,Macedo AM,Luquetti AO,Silva SA,Andrade AL

更新日期：2003-11-01 00:00:00

abstract：:A cluster of methicillin-resistant Staphylococcus aureus (MRSA) infections among patients on an intensive care unit (ICU) was detected by routine infection control surveillance. In the period from 5 January to 22 June 1995, 10 patients on the ICU and a further 6 patients (5 on one ward that had received colonized pati...

journal_title：Journal of clinical microbiology

authors： Tambic A,Power EG,Talsania H,Anthony RM,French GL

更新日期：1997-12-01 00:00:00

abstract：:The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regula...

journal_title：Journal of clinical microbiology

authors： Kurzai O,Heinz WJ,Sullivan DJ,Coleman DC,Frosch M,Mühlschlegel FA

更新日期：1999-05-01 00:00:00

abstract：:The CLSI (formerly NCCLS) M38-A document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. A multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (MICs and m...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Fothergill A,Ghannoum M,Manavathu E,Ostrosky-Zeichner L,Pfaller MA,Rinaldi MG,Schell W,Walsh TJ

更新日期：2007-07-01 00:00:00

abstract：:The nucleic acid sequence of the putative 5'-untranslated (5PUT) region of hepatitis C virus (HCV), determined for samples obtained from a variety of geographic origins, was found to be over 98% conserved among all isolates. On the basis of this signature sequence for HCV, a viral RNA assay was developed by using cDNA...

journal_title：Journal of clinical microbiology

authors： Cha TA,Kolberg J,Irvine B,Stempien M,Beall E,Yano M,Choo QL,Houghton M,Kuo G,Han JH

更新日期：1991-11-01 00:00:00

abstract：:The heterogeneity and the increasing clinical importance of the Enterobacter cloacae complex have often been discussed. However, little is known about molecular factors causing pathogenicity within this nomenspecies. Here, we analyzed the genetic differences between an avirulent plant isolate and a pathogenic strain c...

journal_title：Journal of clinical microbiology

authors： Kremer AN,Hoffmann H

更新日期：2012-10-01 00:00:00

abstract：:We describe a method for the simultaneous identification of up to three Mycoplasma species by the use of contrast-labeled fluorescent antibodies against two species and peroxidase-labeled antibody against a third species. The procedure enabled the rapid identification of colonies of three artificially mixed avian Myco...

journal_title：Journal of clinical microbiology

authors： Bencina D,Bradbury JM

更新日期：1992-02-01 00:00:00

abstract：:A 49-year-old woman with a 15-year history of HIV-hepatitis C virus coinfection had aortic valve and ascending aorta replacement in 2007. She presented with abdominal pain, episodic diarrhea, and profuse sweating in 2010. Thoracoabdominal positron emission tomography-computed tomography finally suggested infectious ao...

journal_title：Journal of clinical microbiology

authors： Rougemont M,Ratib O,Wintsch J,Schrenzel J,Hirschel B

更新日期：2013-08-01 00:00:00

abstract：:A simple, relatively rapid silver impregnation stain has been found to stain Legionella pneumophila effectively in paraffin-embedded tissue sections while permitting visualization of histological detail. It may also be used to stain the organism in body fluids. The stain is not specific and thus must be confirmed by d...

journal_title：Journal of clinical microbiology

authors： Faine S,Edelstein P,Kirby BD,Finegold SM

更新日期：1979-07-01 00:00:00

abstract：:A survey in 2000 to detect methicillin-resistant Staphylococcus aureus (MRSA) colonization in Vancouver downtown east side injection drug users (IDUs) revealed an MRSA nasal colonization incidence of 7.4%. This is a follow-up study to determine the current prevalence of MRSA colonization and to further characterize th...

journal_title：Journal of clinical microbiology

authors： Al-Rawahi GN,Schreader AG,Porter SD,Roscoe DL,Gustafson R,Bryce EA

更新日期：2008-02-01 00:00:00

abstract：:Levels of amikacin in serum were determined in 106 serum specimens by a latex agglutination inhibition card test and by radioimmunoassay (RIA). Linear regression analysis demonstrated a high degree of correlation between the two assays (latex = 0.95 (RIA) + 0.69; r = 0.97). Assay of three control sera containing 7.5, ...

journal_title：Journal of clinical microbiology

authors： Khabbaz RF,Standiford HC,Bernstein D,Nipper HC,Tatem BA,Smalls U,Drusano GL,Caplan E

更新日期：1985-11-01 00:00:00

abstract：:Coprological examination based on egg detection in stool samples is currently used as the gold standard for the diagnosis of human fascioliasis. However, this method is not effective during the acute phase of the disease and has poor sensitivity during the chronic phase. Serodiagnosis has become an excellent alternati...

journal_title：Journal of clinical microbiology

authors： Cabán-Hernández K,Gaudier JF,Ruiz-Jiménez C,Espino AM

更新日期：2014-03-01 00:00:00

abstract：:We retrospectively compared the utility of a fungal isolation device (Isolator) versus conventional techniques for recovering fungal organisms from blood cultures obtained from neutropenic cancer patients. Positive cultures were deemed true pathogens, possible pathogens, or contaminants according to laboratory and cli...

journal_title：Journal of clinical microbiology

authors： Creger RJ,Weeman KE,Jacobs MR,Morrissey A,Parker P,Fox RM,Lazarus HM

更新日期：1998-01-01 00:00:00

abstract：:Widespread use of conjugate pneumococcal polysaccharide-protein vaccines may alter the spectrum of pneumococci producing invasive disease. Novel sensitive diagnostic methods would be valuable for monitoring the epidemiology of pneumococcal disease within populations and vaccine recipients. Ideally, these methods shoul...

journal_title：Journal of clinical microbiology

authors： Leeming JP,Cartwright K,Morris R,Martin SA,Smith MD,South-West Pneumococcus Study Group.

更新日期：2005-10-01 00:00:00

abstract：:The factors that regulate cytomegalovirus (CMV) excretion from the genitourinary tract are poorly understood. To assess the role of cell-mediated immunity in such excretion, a CMV-specific mononuclear blastogenesis assay was used to study a predominantly lower-socioeconomic-status population of 92 healthy nonpregnant ...

journal_title：Journal of clinical microbiology

authors： Faix RG,Zweig SE,Kummer JF,Moore D,Lang DJ

更新日期：1983-04-01 00:00:00

abstract：:Bacteriological and morphological studies of 38 brown pigment common duct stones were performed. Stone cultures were positive for bacteria in 80.5% of those studied. Enterococci were the most common organisms that were isolated. Scanning electron microscopy showed the presence of bacteria in 84.2% of the stones. The b...

journal_title：Journal of clinical microbiology

authors： Leung JW,Sung JY,Costerton JW

更新日期：1989-05-01 00:00:00

abstract：:Rapid and accurate tests are currently needed to identify individuals with high levels of Loa loa microfilaria (mf), so that these individuals may be excluded from mass ivermectin administration campaigns against onchocerciasis and lymphatic filariasis being conducted in areas where Onchocerca volvulus, Wuchereria ban...

journal_title：Journal of clinical microbiology

authors： Drame PM,Fink DL,Kamgno J,Herrick JA,Nutman TB

更新日期：2014-06-01 00:00:00

abstract：:By the use of two reference procedures, an indirect hemagglutination assay and a complement fixation test, the presence or absence of cytomegalovirus (CMV) antibody was determined for 221 human sera. Ninety-nine sera (44.8%) were found to contain CMV antibody. The remaining 122 sera (55.2%) lacked detectable CMV antib...

journal_title：Journal of clinical microbiology

authors： Cheeseman SH,Doern GV,Ferriani RA,Keville MW,McGraw BR,Stewart JA

更新日期：1984-07-01 00:00:00

abstract：:Candida samples were taken over a period of 2 years from 54 human immunodeficiency virus (HIV)-positive asymptomatic subjects to evaluate changes in yeast carriage, intensity of carriage, and genotype over time. Overall, we found that HIV-positive patients with CD4(+)-cell counts of between 200 and 400/microl had sign...

journal_title：Journal of clinical microbiology

authors： Vargas KG,Joly S

更新日期：2002-02-01 00:00:00

abstract：:Despite the high virological response rates achieved with current directly acting antiviral agents (DAAs) against hepatitis C virus (HCV), around 2% to 5% of treated patients do not achieve a sustained viral response. The identification of amino acid substitutions associated with treatment failure requires analytical ...

journal_title：Journal of clinical microbiology

authors： Soria ME,García-Crespo C,Martínez-González B,Vázquez-Sirvent L,Lobo-Vega R,de Ávila AI,Gallego I,Chen Q,García-Cehic D,Llorens-Revull M,Briones C,Gómez J,Ferrer-Orta C,Verdaguer N,Gregori J,Rodríguez-Frías F,Buti M,Este

更新日期：2020-11-18 00:00:00

abstract：:A reversed passive latex agglutination (RPLA) assay for determining the toxigenicity of Corynebacterium diphtheriae is presented. Rabbit antitoxin antiserum was raised by using commercially available diphtheria toxoid. This antiserum reacted with the diphtheria toxin when the culture supernatant was assayed by Western...

journal_title：Journal of clinical microbiology

authors： Toma C,Sisavath L,Iwanaga M

更新日期：1997-12-01 00:00:00

abstract：:When the standard procedure for determining antibiotic susceptibility of bacteria is used, the results are delayed, especially for bacteria that grow slowly, such as Helicobacter pylori. Treatment for this bacterium may involve clarithromycin, a compound for which resistance has been associated with point mutations on...

journal_title：Journal of clinical microbiology

authors： Pina M,Occhialini A,Monteiro L,Doermann HP,Mégraud F

更新日期：1998-11-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) variant B is frequently identified in peripheral blood, but identification of HHV-6 variant A is relatively rare. We devised a PCR-based method for sensitive, simultaneous detection of both HHV-6 variants. The method was applied to 34 lung tissue specimens that were previously shown to cont...

journal_title：Journal of clinical microbiology

authors： Cone RW,Huang ML,Hackman RC,Corey L

更新日期：1996-04-01 00:00:00

abstract：:The new anaerobe and Corynebacterium (ANC) identification card for Vitek 2 was compared with a 16S rRNA gene sequencing (16S) reference method for accuracy in the identification of corynebacteria and anaerobic species. Testing was performed on a Vitek 2 XL system with modified software at three clinical trial laborato...

journal_title：Journal of clinical microbiology

authors： Rennie RP,Brosnikoff C,Turnbull L,Reller LB,Mirrett S,Janda W,Ristow K,Krilcich A

更新日期：2008-08-01 00:00:00

abstract：:A strain of Escherichia coli isolated from a child with diarrhea was biochemically similar and antigenically identical (O and K antigens) to the standard strain of E. coli O group 29 and was positive in the Serény test for invasiveness, which suggests that it can cause a Shigella-like illness. ...

journal_title：Journal of clinical microbiology

authors： Toledo MR,Reis MH,Almeida RG,Trabulsi LR

更新日期：1979-02-01 00:00:00

abstract：:Growth curves of the yeast form of Paracoccidioides brasiliensis B-339 based on total and viable cell counts were determined. Crude culture filtrate antigens were obtained after 7, 10, 15, 20, 25, and 30 days of incubation. Different patterns of proteins were obtained by affinity chromatography on Sepharose 4B-immunog...

journal_title：Journal of clinical microbiology

authors： De Camargo Z,Unterkircher C,Campoy SP,Travassos LR

更新日期：1988-10-01 00:00:00

abstract：:A latex agglutination test was evaluated as a method for detection and titration of antiplague antibodies. Slide and microtiter techniques using polystyrene latex particles coated with specific fraction-I-antigen of Yersinia pestis were found to be comparable in specificity and sensitivity to serological tests commonl...

journal_title：Journal of clinical microbiology

authors： Suzuki S,Sakakibara H,Hotta S

更新日期：1977-10-01 00:00:00