Multicenter evaluation of the Vitek 2 anaerobe and Corynebacterium identification card.

abstract：:Brucellosis in Argentina is currently investigated in bank donor blood by the standard plate agglutination test (PAT). This study evaluated the buffered plate antigen test (BPA), now used to screen for bovine brucellosis, as a screen for human disease. Of 57 sera from patients with culture-confirmed brucellosis, 100% ...

journal_title：Journal of clinical microbiology

authors： Lucero NE,Bolpe JE

更新日期：1998-05-01 00:00:00

abstract：:Species of the genus Bartonella are currently recognized in growing numbers and are involved in an increasing variety of human diseases, mainly trench fever, Carrion's disease, bacillary angiomatosis, endocarditis, cat scratch disease, neuroretinitis, and asymptomatic bacteremia. Such a wide spectrum of infections mak...

journal_title：Journal of clinical microbiology

authors： Houpikian P,Raoult D

更新日期：2001-08-01 00:00:00

abstract：:A 44-year-old diabetic female presented to a hospital in Jamaica with thermal burns. Trichosporon asahii was isolated from facial wounds, sputum, and a meningeal swab. Dissemination of the fungus was demonstrated in stained histological sections of the meninges and a brain abscess at autopsy. Pure growth of the fungus...

journal_title：Journal of clinical microbiology

authors： Heslop OD,Nyi Nyi MP,Abbott SP,Rainford LE,Castle DM,Coard KC

更新日期：2011-12-01 00:00:00

abstract：:An alpha-toxin disk test is described in which group B streptococci completed the hemolysis of sheep erythrocytes partially lysed by the alpha-toxin of Clostridium perfringens. The test was performed satisfactorily on the sheep blood agar primary isolation plate, as well as on pure cultures. A total of 95% of strains ...

journal_title：Journal of clinical microbiology

authors： Smith JA,Ngui-Yen JH

更新日期：1980-07-01 00:00:00

abstract：:We examined the blastogenic responses of lymphocytes to Staphylococcus aureus antigen in 49 uninfected non-insulin-dependent diabetic subjects and in 56 uninfected nondiabetic subjects. We found a significantly decreased response to this antigen in the diabetic patients (P less than 0.05). Diabetics whose blood sugar ...

journal_title：Journal of clinical microbiology

authors： Casey J,Sturm C Jr

更新日期：1982-01-01 00:00:00

abstract：:Members of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiot...

journal_title：Journal of clinical microbiology

authors： Roggenkamp A,Leitritz L,Baus K,Falsen E,Heesemann J

更新日期：1998-10-01 00:00:00

abstract：:The increasing incidence of invasive aspergillosis, a life-threatening infection in immunocompromised patients, emphasizes the need to improve the diagnostic tools for this disease. We established a LightCycler-based real-time PCR assay to detect and quantify rapidly, specifically, and sensitively Aspergillus fumigatu...

journal_title：Journal of clinical microbiology

authors： Spiess B,Buchheidt D,Baust C,Skladny H,Seifarth W,Zeilfelder U,Leib-Mösch C,Mörz H,Hehlmann R

更新日期：2003-05-01 00:00:00

abstract：:Rapid pathogen identification can alter antibiotic prescribing practices if interpreted correctly. Microbiology reporting can be difficult to understand, and new technology has made it more challenging. Nebraska Medicine recently implemented the BioFire FilmArray blood culture identification panel (BCID) coupled with ...

journal_title：Journal of clinical microbiology

authors： Donner LM,Campbell WS,Lyden E,Van Schooneveld TC

更新日期：2017-05-01 00:00:00

abstract：:Sequence analysis of an 850-bp fragment internal to the aspecific drug efflux gene ade B revealed 11 sequence types (STs) among a collection of 50 multidrug-resistant Acinetobacter baumannii (MDRAB) strains, including members of pan-European clones I, II, and III. The delineation of STs conformed with the intraspecifi...

journal_title：Journal of clinical microbiology

authors： Huys G,Cnockaert M,Nemec A,Swings J

更新日期：2005-10-01 00:00:00

abstract：:Respiratory diphtheria, characterized by a firmly adherent pseudomembrane, is caused by toxin-producing strains of Corynebacterium diphtheriae, with similar illness produced occasionally by toxigenic Corynebacterium ulcerans or, rarely, Corynebacterium pseudotuberculosis While diphtheria laboratory confirmation requir...

journal_title：Journal of clinical microbiology

authors： Williams MM,Waller JL,Aneke JS,Weigand MR,Diaz MH,Bowden KE,Simon AK,Peng Y,Xiaoli L,Cassiday PK,Winchell J,Tondella ML

更新日期：2020-09-22 00:00:00

abstract：:Hepatitis C virus (HCV) infection typically leads to antibody response within weeks after primary infection. Here, we describe the case of a child infected with HCV by mother-to-child transmission who remained persistently seronegative despite the presence of high levels of circulating HCV RNA. ...

journal_title：Journal of clinical microbiology

authors： Larouche A,Gaëtan G,El-Bilali N,Quesnel-Vallières M,Martin SR,Alvarez F,Shoukry NH,Soudeyns H

更新日期：2012-07-01 00:00:00

abstract：:The use of counterimmunoelectrophoresis (CIE) for detection of serum antibodies to staphylococcal teichoic acids was evaluated against teichoic acids prepared by sonic treatment or lysostaphin extraction of Staphylococcus aureus (Lafferty strain). Of 54 patient sera from suspected cases of staphylococcal endocarditis,...

journal_title：Journal of clinical microbiology

authors： Leffell MS,Folds JD,Wasilauskas B

更新日期：1978-11-01 00:00:00

abstract：:The increasing number of multidrug-resistant Mycobacterium tuberculosis strains has stimulated the interest of investigators in finding a rapid method for susceptibility testing. We used commercially available rRNA DNA-bioluminescence-labelled probes (Accu-Probe, Gen Probe, Inc. San Diego, Calif.) for this purpose. Th...

journal_title：Journal of clinical microbiology

authors： Martin-Casabona N,Xairó Mimó D,González T,Rossello J,Arcalis L

更新日期：1997-10-01 00:00:00

abstract：:Diagnostic laboratories are under increasing pressure to improve and expand their services. Greater flexibility in sample processing is a critical factor that can improve the time to results while reducing reagent waste, making laboratories more efficient and cost-effective. The introduction of the Abbott mPLUS featur...

journal_title：Journal of clinical microbiology

authors： Lucic D,Jones S,Wiesneth R,Barry C,Webb E,Belova L,Dolan P,Ho S,Abravaya K,Cloherty G

更新日期：2013-12-01 00:00:00

abstract：:A new phenomenon of synergistic hemolysis by Clostridium perfringens alpha-toxin and the streptococcal CAMP factor on human and guinea pig erythrocytes is described. A possible mode of action of the CAMP factors is suggested. On human blood agar all of the tested isolates of group B streptococci gave an arrowhead-shap...

journal_title：Journal of clinical microbiology

authors： Gubash SM

更新日期：1978-11-01 00:00:00

abstract：:To study the population structure of Enterococcus faecalis from Polish hospitals, 291 isolates were typed by pulsed-field gel electrophoresis and a novel multilocus sequence typing scheme (P. Ruiz-Garbajosa et al., J. Clin. Microbiol. 44:2220-2228, 2006). The isolates originated from geographically widespread medical ...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Pietras Z,Daniłowicz E,Jakubczak A,Gniadkowski M,Hryniewicz W,Willems RJ

更新日期：2007-01-01 00:00:00

abstract：:Owing to the difficulties in isolating the virus and the lack of routine surveillance, the clinical significance of human parainfluenza virus 4 (HPIV-4) is less well defined than that of the other human parainfluenza viruses. We describe the first outbreak of HPIV-4 infection in a developmental disabilities unit, invo...

journal_title：Journal of clinical microbiology

authors： Lau SK,To WK,Tse PW,Chan AK,Woo PC,Tsoi HW,Leung AF,Li KS,Chan PK,Lim WW,Yung RW,Chan KH,Yuen KY

更新日期：2005-09-01 00:00:00

abstract：:A cross-reaction between Acinetobacter calcoaceticus and chlamydiae is described. A water-soluble, heat stable, non-dialyzable antigen was extracted from Acinetobacter species by boiling. This antigen fixed complement in the presence of homologous hyperimmune sera from rabbits or guinea pigs and in the presence of het...

journal_title：Journal of clinical microbiology

authors： Brade H,Brunner H

更新日期：1979-12-01 00:00:00

abstract：:During a 3-year period, all Pasteurella strains recovered at the Clinical Microbiological Laboratory, Lund, Sweden, were studied biochemically with respect to their relationship to the recently described taxa of this genus. Of 159 strains recovered from 146 infected humans, 95 were identified as Pasteurella multocida ...

journal_title：Journal of clinical microbiology

authors： Holst E,Rollof J,Larsson L,Nielsen JP

更新日期：1992-11-01 00:00:00

abstract：:The complete nucleotide sequence of one of the immediate-early genes of human herpesvirus 6 variant B was determined and compared with that of variant A reported by Martin et al. (M.D. Martin, J. Nicholas, B. J. Thomson, C. Newman, and R. W. Honess, J. Virol. 65:5381-5390, 1991). While it was reported that two open re...

journal_title：Journal of clinical microbiology

authors： Yamamoto T,Mukai T,Kondo K,Yamanishi K

更新日期：1994-02-01 00:00:00

abstract：:To study whether pathogenic clusters of Borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n = 49) with various clinical entities and from ticks (n = 83) or dogs (n = 4) from different geographic regions, by randomly amplified polymorphic DNA (RAPD) fingerprint...

journal_title：Journal of clinical microbiology

authors： Wang G,van Dam AP,Spanjaard L,Dankert J

更新日期：1998-03-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response. Variability of the gG-1 gene among wild-type strains may be a factor of importance for a reliable serodiagnosis and typing of HSV-1 isolates. Here, we used a gG-1 type-spec...

journal_title：Journal of clinical microbiology

authors： Rekabdar E,Tunbäck P,Liljeqvist JA,Lindh M,Bergström T

更新日期：2002-09-01 00:00:00

abstract：:In an epidemiological study, nine isolates of dematiaceous fungi were recovered from the interior of a local feed and seed warehouse. Sample sites include brick walls and floors. Air samples also were included. Samples were collected in saline and plated on Mycobiotic and Sabouraud agar. The nine dematiaceous fungi re...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Kerkering TM,Shadomy HJ

更新日期：1982-04-01 00:00:00

abstract：:Previous studies have reported the increased sensitivity of PCR targeting AF146527 over that of PCR targeting the B1 gene for diagnosis of toxoplasmosis. The present study suggests that the AF146527 element was absent in 4.8% of human Toxoplasma gondii-positive samples tested. The data argue that the B1 gene may be th...

journal_title：Journal of clinical microbiology

authors： Wahab T,Edvinsson B,Palm D,Lindh J

更新日期：2010-02-01 00:00:00

abstract：:Detection of human cytomegalovirus (CMV) DNA in clinical specimens is considered a cornerstone in the diagnosis of CMV disease. The aim of this study was to evaluate a newly designed LightCycler-based quantitative CMV PCR. Specimens of human origin (n = 200) were tested using the LightCycler PCR, the quantitative COBA...

journal_title：Journal of clinical microbiology

authors： Schaade L,Kockelkorn P,Ritter K,Kleines M

更新日期：2000-11-01 00:00:00

abstract：:We report the first case of disseminated infection with both Actinomyces graevenitzii and Mycobacterium tuberculosis and review the medical literature. Concomitant actinomycosis and tuberculosis is very rare. The potential of the facultatively aerobic, newly described A. graevenitzii for disseminated invasive infectio...

journal_title：Journal of clinical microbiology

authors： Tietz A,Aldridge KE,Figueroa JE

更新日期：2005-06-01 00:00:00

abstract：:The frequencies of 10 opportunistic DNA viruses were determined by multiplex real-time PCR in paired cerebrospinal fluid (CSF) and brain tissue of HIV-infected individuals. In the CSF, viruses were detectable in 45/55 cases: JC virus (JCV) in 62%, Epstein-Barr virus (EBV) in 44%, cytomegalovirus (CMV) in 25%, varicell...

journal_title：Journal of clinical microbiology

authors： Kannangai R,Sachithanandham J,Mahadevan A,Abraham AM,Sridharan G,Desai A,Ravi V,Shankar SK

更新日期：2013-03-01 00:00:00

abstract：:Pneumocystis jirovecii pneumonia (PCP) is an opportunistic infection with airborne transmission and remains a major cause of respiratory illness among immunocompromised individuals. In recent years, several outbreaks of PCP, occurring mostly in kidney transplant recipients, have been reported. Currently, multilocus se...

journal_title：Journal of clinical microbiology

authors： Maitte C,Leterrier M,Le Pape P,Miegeville M,Morio F

更新日期：2013-09-01 00:00:00

abstract：:The performance characteristics of the Gen-Probe Probe Competition Assay (PCA) used in conjunction with the Gen-Probe PACE 2 and 2C direct detection assays for Chlamydia trachomatis were examined. Data collected by five public health laboratories by using the Gen-Probe PACE 2 were pooled and analyzed. Of 25,081 endoce...

journal_title：Journal of clinical microbiology

authors： Beebe JL,Sharpton TR,Zanto SN,Steece RS,Rogers C,Mottice SL

更新日期：1997-02-01 00:00:00

abstract：:A mycobacterium isolated from a clinical sample of an AIDS patient was identified as Mycobacterium interjectum by direct 16S rRNA sequence determination. High-performance liquid chromatography, however, revealed a mycolic acid pattern which was different from the one shared by the previously analyzed strains of this s...

journal_title：Journal of clinical microbiology

authors： Tortoli E,Kirschner P,Bartoloni A,Burrini C,Manfrin V,Mantella A,Scagnelli M,Scarparo C,Simonetti MT,Böttger EC

更新日期：1996-09-01 00:00:00