Impact of the New Abbott mPLUS feature on clinical laboratory efficiencies of abbott RealTime assays for detection of HIV-1, Hepatitis C Virus, Hepatitis B Virus, Chlamydia trachomatis, and Neisseria gonorrhoeae.

abstract：:Infections due to small-colony variants (SCVs) of Staphylococcus aureus in patients with chronic and recurrent infections are an emerging problem; however, studies with this subpopulation are hampered by the fact that SCVs may exhibit unstable phenotypes, making them difficult to study, particularly in broth media. In...

journal_title：Journal of clinical microbiology

authors： Becker K,Laham NA,Fegeler W,Proctor RA,Peters G,von Eiff C

更新日期：2006-09-01 00:00:00

abstract：:Combination therapy can successfully suppress human immunodeficiency virus (HIV) replication in patients but selects for drug resistance, requiring subsequent resistance-guided therapeutic changes. This report describes the development and validation of a novel assay that offers a uniform method to measure susceptibil...

journal_title：Journal of clinical microbiology

authors： Covens K,Dekeersmaeker N,Schrooten Y,Weber J,Schols D,Quiñones-Mateu ME,Vandamme AM,Van Laethem K

更新日期：2009-07-01 00:00:00

abstract：:Whole-cell sonicates of Treponema pallidum, Nichols strain, were evaluated in an enzyme-linked immunosorbent assay (Elisa) for syphilis, and results were read in a Dynatek Microelisa Reader. The antigen was evaluated with sera from patients with syphilis, persons presumed normal, and biological false-positives. Two hu...

journal_title：Journal of clinical microbiology

authors： Pope V,Hunter EF,Feeley JC

更新日期：1982-04-01 00:00:00

abstract：:Monoclonal antibodies reactive with three different viral polypeptides were evaluated singly and in combination as the capture antibody(s) in an enzyme-linked immunosorbent assay system for the detection of bovine enteric coronavirus. Similar levels of sensitivity were found for all combinations tested. A sensitive, h...

journal_title：Journal of clinical microbiology

authors： Crouch CF,Raybould TJ,Acres SD

更新日期：1984-03-01 00:00:00

abstract：:MICs and minimal bactericidal concentrations (MBCs) were evaluated for the four antibiotics azithromycin, amoxicillin, ceftriaxone, and doxycycline against the three main genospecies of Borrelia burgdorferi sensu lato. In MBC testing, statistically significant differences between the genospecies could be found in 7 ou...

journal_title：Journal of clinical microbiology

authors： Sicklinger M,Wienecke R,Neubert U

更新日期：2003-04-01 00:00:00

abstract：:The epidemiology of new acquisition of antibiotic-resistant organisms (AROs) in community-based skilled nursing facilities (SNFs) is not well studied. To define the incidence, persistence of, and time to new colonization with methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), a...

journal_title：Journal of clinical microbiology

authors： Fisch J,Lansing B,Wang L,Symons K,Cherian K,McNamara S,Mody L

更新日期：2012-05-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently been described as a fast and inexpensive method for the identification of mycobacteria. Although mycobacteria require extraction prior to MALDI-TOF MS analysis, previously published protocols have been relatively c...

journal_title：Journal of clinical microbiology

authors： Mather CA,Rivera SF,Butler-Wu SM

更新日期：2014-01-01 00:00:00

abstract：:We studied nine solid and two liquid media for their suitability to select Aeromonas and Plesiomonas spp. from human stools, using artificially contaminated samples as well as 254 samples from outpatients with and without diarrhea. Media with optimal sensitivity and specificity for Aeromonas spp. were alkaline peptone...

journal_title：Journal of clinical microbiology

authors： von Graevenitz A,Bucher C

更新日期：1983-01-01 00:00:00

abstract：:Molecular techniques such as spa typing and multilocus sequence typing use DNA sequence data for differentiating Staphylococcus aureus isolates. Although spa typing is capable of detecting both genetic micro- and macrovariation, it has less discriminatory power than the more labor-intensive pulsed-field gel electropho...

journal_title：Journal of clinical microbiology

authors： Koreen L,Ramaswamy SV,Naidich S,Koreen IV,Graff GR,Graviss EA,Kreiswirth BN

更新日期：2005-08-01 00:00:00

abstract：:In 1991, multiresistant Escherichia coli O78:H10 strains caused an outbreak of urinary tract infections in Copenhagen, Denmark. The phylogenetic origin, clonal background, and virulence characteristics of the outbreak isolates, and their relationship to nonoutbreak O78:H10 strains according to these traits and resista...

journal_title：Journal of clinical microbiology

authors： Olesen B,Scheutz F,Andersen RL,Menard M,Boisen N,Johnston B,Hansen DS,Krogfelt KA,Nataro JP,Johnson JR

更新日期：2012-11-01 00:00:00

abstract：:We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suita...

journal_title：Journal of clinical microbiology

authors： Hrncirova K,Lengerova M,Kocmanova I,Racil Z,Volfova P,Palousova D,Moulis M,Weinbergerova B,Winterova J,Toskova M,Pospisilova S,Mayer J

更新日期：2010-09-01 00:00:00

abstract：:Meningococcal disease is normally suspected on clinical grounds and is confirmed by isolation of Neisseria meningitidis bacteria from blood or cerebrospinal fluid or, more recently, by serology or PCR of cerebrospinal fluid. Achieving confirmation of a clinical diagnosis of meningococcal disease has become more diffic...

journal_title：Journal of clinical microbiology

authors： Newcombe J,Cartwright K,Palmer WH,McFadden J

更新日期：1996-07-01 00:00:00

abstract：:We present the first case of severe pneumonia possibly caused by Leptotrichia species with oral bacteria. This was found in a healthy but elderly subject whose bronchoalveolar lavage fluid was analyzed by 16S rRNA gene sequencing analysis. The combination of this method and microscopic observation provided useful info...

journal_title：Journal of clinical microbiology

authors： Kawanami T,Fukuda K,Yatera K,Kido T,Yoshii C,Taniguchi H,Kido M

更新日期：2009-02-01 00:00:00

abstract：:Conventional culture techniques are limited in the ability to detect multiple Streptococcus pneumoniae serotypes in nasopharyngeal (NP) secretions. We developed an immunoblot (IB) method with monoclonal antibodies (MAbs) to detect S. pneumoniae and to identify serotypes. NP specimens stored in skim milk-tryptone-gluco...

journal_title：Journal of clinical microbiology

authors： Bronsdon MA,O'Brien KL,Facklam RR,Whitney CG,Schwartz B,Carlone GM

更新日期：2004-04-01 00:00:00

abstract：:Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

journal_title：Journal of clinical microbiology

authors： Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

更新日期：2011-09-01 00:00:00

abstract：:Pneumocystis jirovecii pneumonia (PCP) is a life-threatening infection in immunocompromised patients. Quantitative real-time PCR (qPCR) is more sensitive than microscopic examination for the detection of P. jirovecii but also detects colonized patients. Hence, its positive predictive value (PPV) needs evaluation. In t...

journal_title：Journal of clinical microbiology

authors： Robert-Gangneux F,Belaz S,Revest M,Tattevin P,Jouneau S,Decaux O,Chevrier S,Le Tulzo Y,Gangneux JP

更新日期：2014-09-01 00:00:00

abstract：:A replicator method for screening Staphylococcus aureus isolates for synergistic hemolytic activity is described. The technique should also be useful for assessing the hemolytic activity of staphylococci and streptococci on different lots or brands of blood agar because the inoculum and the distances between spot inoc...

journal_title：Journal of clinical microbiology

authors： Boyce JM

更新日期：1985-05-01 00:00:00

abstract：:Capnocytophaga is a genus of gram-negative rods involved mainly in bacteremia in immunocompromised hosts. Here we report a case of Capnocytophaga peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis. The disease appeared as a superinfection during antimicrobial therapy of a previous episode of...

journal_title：Journal of clinical microbiology

authors： Esteban J,Albalate M,Caramelo C,Reyero A,Carriazo MA,Hernandez J,Ortiz A,Soriano F

更新日期：1995-09-01 00:00:00

abstract：:Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae generally cannot be treated with penicillins and cephalosporins. However, some later-generation cephalosporins, including cefepime, are poorly hydrolyzed by specific ESBL enzymes, and certain strains demonstrate in vitro susceptibility to these agent...

journal_title：Journal of clinical microbiology

authors： Smith KP,Brennan-Krohn T,Weir S,Kirby JE

更新日期：2017-02-01 00:00:00

abstract：:We report an outbreak of Saccharomyces cerevisiae subtype boulardii fungemia among three intensive care unit roommates of patients receiving lyophilized preparations of this fungus. The fungemia was probably due to central venous catheter contamination and resolved after fluconazole treatment. The need for stringent a...

journal_title：Journal of clinical microbiology

authors： Cassone M,Serra P,Mondello F,Girolamo A,Scafetti S,Pistella E,Venditti M

更新日期：2003-11-01 00:00:00

abstract：:Clostridium difficile is the bacterium most commonly surmised to cause antimicrobial- and hospital-associated diarrhea in developed countries worldwide, and such infections are thought to be increasing in frequency and severity. A laboratory-based study was carried out to characterize C. difficile strains isolated fro...

journal_title：Journal of clinical microbiology

authors： Martin H,Willey B,Low DE,Staempfli HR,McGeer A,Boerlin P,Mulvey M,Weese JS

更新日期：2008-09-01 00:00:00

abstract：:Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not i...

journal_title：Journal of clinical microbiology

authors： Funk ND,Tabatabai LB,Elzer PH,Hagius SD,Martin BM,Hoffman LJ

更新日期：2005-02-01 00:00:00

abstract：:Bacteriuria due to Salmonella typhi usually occurs following recent typhoid fever or in chronic carrier states. Data from 18 patients with S. typhi bacteriuria, seen during 5 years, were analyzed. Fourteen patients had localized urinary tract infection due to S. typhi. Four others had bacteriuria, probably associated ...

journal_title：Journal of clinical microbiology

authors： Mathai E,John TJ,Rani M,Mathai D,Chacko N,Nath V,Cherian AM

更新日期：1995-07-01 00:00:00

abstract：:Matched vaginal and cervical specimens from 96 subjects were analyzed by quantitative PCR for the presence and concentration of bacterial vaginosis-associated microbes and commensal Lactobacillus spp. Detection of these microbes was 92% concordant, indicating that microbial floras at these body sites are generally sim...

journal_title：Journal of clinical microbiology

authors： Smith WL,Hedges SR,Mordechai E,Adelson ME,Trama JP,Gygax SE,Kaunitz AM,Hilbert DW

更新日期：2014-08-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome has been devastating the swine industry since the late 1980s. The disease has been controlled, to some extent, through the use of modified live-attenuated (MLV) vaccines once available. However, such a practice periodically resulted in isolation or detection of vaccine-lik...

journal_title：Journal of clinical microbiology

authors： Key KF,Guenette DK,Yoon KJ,Halbur PG,Toth TE,Meng XJ

更新日期：2003-06-01 00:00:00

abstract：:The API NeIdent system (Analytab Products, Plainview, N.Y.) was evaluated for identifying Neisseria spp. and Branhamella catarrhalis commonly isolated from clinical specimens. The system identified 90% of 303 Neisseria gonorrhoeae isolates, 71% of 113 Neisseria meningitidis isolates, and 63% of 16 Neisseria lactamica ...

journal_title：Journal of clinical microbiology

authors： Janda WM,Morello JA,Bohnhoff M

更新日期：1984-03-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) represent important tools to diagnose malaria infection. To improve understanding of the variable performance of RDTs that detect the major target in Plasmodium falciparum, namely, histidine-rich protein 2 (HRP2), and to inform the design of better tests, we undertook detailed mapping of ...

journal_title：Journal of clinical microbiology

authors： Lee N,Gatton ML,Pelecanos A,Bubb M,Gonzalez I,Bell D,Cheng Q,McCarthy JS

更新日期：2012-04-01 00:00:00

abstract：:Polyomavirus BK (BKV) causes asymptomatic latent infection in the human host that is reactivated during periods of immune suppression. Detection by conventional tube cell culture is difficult and time consuming because BKV exhibits slow growth with late (14 to 28 days) and subtle cytopathic effects. We developed a she...

journal_title：Journal of clinical microbiology

authors： Marshall WF,Telenti A,Proper J,Aksamit AJ,Smith TF

更新日期：1990-07-01 00:00:00

abstract：:Three commercial chromogenic agar media were evaluated for Streptococcus agalactiae screening in 200 vaginal swabs from pregnant women. The sensitivity and specificity were 94.3% and 100% for Granada medium (bioMérieux), 100% and 90.3% for Brilliance GBS medium (Thermo Fisher Scientific), and 100% and 98.8% for ChromI...

journal_title：Journal of clinical microbiology

authors： Verhoeven PO,Noyel P,Bonneau J,Carricajo A,Fonsale N,Ros A,Pozzetto B,Grattard F

更新日期：2014-03-01 00:00:00

abstract：:The clinical observation has been made that there might be an unacceptable number of false-positive enzyme immunoassay (EIA) test results for IgM among persons suspected of having coccidioidomycosis. Patients with a positive result for IgM by EIA are thought to have a diagnosis of acute coccidioidomycosis. However, th...

journal_title：Journal of clinical microbiology

authors： Kuberski T,Herrig J,Pappagianis D

更新日期：2010-06-01 00:00:00