Rapid detection of polyomavirus BK by a shell vial cell culture assay.

abstract：:DNA restriction analysis was carried out on a sample of 212 epidemiologically unrelated adenovirus (Ad) strains of serotype 7 isolated in Chile, Uruguay, and Argentina from nasopharyngeal aspirates of children with acute lower respiratory disease between 1984 and 1990. The identified genome types were Ad7b (n = 12), A...

journal_title：Journal of clinical microbiology

authors： Kajon A,Wadell G

更新日期：1994-09-01 00:00:00

abstract：:The automated and computerized AutoMicrobic system (AMS; Vitek Systems, Inc., subsidiary of McDonnell Douglas, Hazelwood, Mo.) was evaluated as a means of identifying the Enterobacteriaceae. The Micro-ID system (General Diagnostics, Morris Plains, N.J.) and, when necessary, conventional tubed media were used for compa...

journal_title：Journal of clinical microbiology

authors： Burdash NM,Teti G,West ME,Bannister ER,Manos JP

更新日期：1981-02-01 00:00:00

abstract：:Influenza epidemics arise through the accumulation of viral genetic changes, culminating in a novel antigenic type that is able to escape host immunity. Following an outbreak of the A/Fujian/411/2002-like strains in Asia, including China, Japan, and South Korea, in 2002, Australia and New Zealand experienced substanti...

journal_title：Journal of clinical microbiology

authors： Kang S,Yang IS,Lee JY,Park Y,Oh HB,Kang C,Kim KH

更新日期：2010-06-01 00:00:00

abstract：:Three molecular typing methods were used to study the relationships among 184 Campylobacter strains isolated from humans, cattle, and chickens. All strains were genotyped by amplified fragment length polymorphism (AFLP) analysis, multilocus sequence typing (MLST), and sequence analysis of a genomic region with short t...

journal_title：Journal of clinical microbiology

authors： Schouls LM,Reulen S,Duim B,Wagenaar JA,Willems RJ,Dingle KE,Colles FM,Van Embden JD

更新日期：2003-01-01 00:00:00

abstract：:Two DNA probes, one 1.1- and one 0.75-kb probe, specific for Serpulina hyodysenteriae were isolated from a genomic library generated from virulent S. hyodysenteriae 5380. These probes are highly specific and react with all S. hyodysenteriae strains tested. Under stringent conditions, the DNA probes did not react with ...

journal_title：Journal of clinical microbiology

authors： Sotiropoulos C,Smith SC,Coloe PJ

更新日期：1993-07-01 00:00:00

abstract：:Marburg virus (MARV) causes a severe hemorrhagic fever in humans with a high mortality rate. The rapid and accurate identification of the virus is required to appropriately provide infection control and outbreak management. Here, we developed and evaluated a one-step reverse transcription-loop-mediated isothermal ampl...

journal_title：Journal of clinical microbiology

authors： Kurosaki Y,Grolla A,Fukuma A,Feldmann H,Yasuda J

更新日期：2010-07-01 00:00:00

abstract：:Immunochromatographic test (ICT) kits for the rapid detection of immunoglobulin G (IgG) and IgM antibodies to Burkholderia pseudomallei were compared to the indirect hemagglutination (IHA) assay. In 138 culture-confirmed melioidosis cases, sensitivities were 80, 77, and 88% for IHA, ICT IgG, and ICT IgM, respectively....

journal_title：Journal of clinical microbiology

authors： O'Brien M,Freeman K,Lum G,Cheng AC,Jacups SP,Currie BJ

更新日期：2004-05-01 00:00:00

abstract：:Family members of hepatitis C virus (HCV)-infected hemodialysis patients had a high rate (8.1%) of HCV infection and were often infected by the same genotype of HCV as the renal patients. Renal patients who had a longer history of hemodialysis or more frequent arteriovenous shunt replacement posed a higher risk to the...

journal_title：Journal of clinical microbiology

authors： Ho MS,Yang CS,Chen PJ,Mau YC

更新日期：1994-11-01 00:00:00

abstract：:The staphylococcal methicillin resistance determinant, mecA, resides on a mobile genetic element, staphylococcus chromosomal cassette mec (SCCmec). The distribution of SCCmec in nature is limited to relatively few clonal complexes of related methicillin-resistant Staphylococcus aureus (MRSA). We have previously report...

journal_title：Journal of clinical microbiology

authors： Katayama Y,Robinson DA,Enright MC,Chambers HF

更新日期：2005-05-01 00:00:00

abstract：:The Clinical and Laboratory Standards Institute (CLSI) has revised several breakpoints since 2010 for bacteria that grow aerobically. In 2019, these revisions include changes to the ciprofloxacin and levofloxacin breakpoints for the Enterobacteriaceae and Pseudomonas aeruginosa, daptomycin breakpoints for Enterococcus...

journal_title：Journal of clinical microbiology

authors： Humphries RM,Abbott AN,Hindler JA

更新日期：2019-05-24 00:00:00

abstract：:Rhodotorula species are emerging pathogens in immunocompromised patients. We report the in vitro activities of eight antifungals against 64 Rhodotorula isolates collected in surveillance programs between 1987 and 2003. Rhodotorula strains are resistant in vitro to fluconazole (MIC at which 50% of the isolates tested a...

journal_title：Journal of clinical microbiology

authors： Diekema DJ,Petroelje B,Messer SA,Hollis RJ,Pfaller MA

更新日期：2005-01-01 00:00:00

abstract：:The sensitive peroxidase-antiperoxidase (PXAPX) method individually and in conjunction with the Papanicolaou (PAP) stain was used to detect herpes simplex virus (HSV) in specimens from human female genitalia. Initial studies using a model system of HSV-1 or HSV-2-infected Vero cells established (i) acetone as the most...

journal_title：Journal of clinical microbiology

authors： Pearson NS,Fleagle G,Docherty JJ

更新日期：1979-11-01 00:00:00

abstract：:Several instruments for automated or semiautomated susceptibility testing are currently available. Three of these instruments, Autobac (General Diagnostics, Warner-Lambert Co., Morris Plains, N.J.), MS-2 (Abbott Laboratories, Dallas, Tex.), and AutoMicrobic system (AMS) (Vitek, Inc., Hazelwood, Mo.) were compared for ...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Latimer JM,Balfour LC

更新日期：1982-05-01 00:00:00

abstract：:A total of 189 Candida albicans isolates have been typed by multilocus enzyme electrophoresis. The results obtained confirm the clonal mode of reproduction of C. albicans. The C. albicans populations found in the oropharynx of human immunodeficiency virus (HIV)-infected patients, in the oropharynx of healthy carriers,...

journal_title：Journal of clinical microbiology

authors： Boerlin P,Boerlin-Petzold F,Goudet J,Durussel C,Pagani JL,Chave JP,Bille J

更新日期：1996-05-01 00:00:00

abstract：:A direct and an indirect enzyme immunoassay (EIA) were evaluated against a standard of electron microscopy after direct ultracentrifugation of the specimen for their performances in detecting rotaviruses. The indirect EIA had variable background activity which influenced test specificity. The indirect EIA control (tes...

journal_title：Journal of clinical microbiology

authors： Hammond GW,Ahluwalia GS,Barker FG,Horsman G,Hazelton PR

更新日期：1982-07-01 00:00:00

abstract：:A set of 104 isolates from human clinical samples from the United States, morphologically compatible with Bipolaris, were morphologically and molecularly identified through the sequence analysis of the internal transcribed space (ITS) region of the nuclear ribosomal DNA (rDNA). The predominant species was Bipolaris sp...

journal_title：Journal of clinical microbiology

authors： da Cunha KC,Sutton DA,Fothergill AW,Cano J,Gené J,Madrid H,De Hoog S,Crous PW,Guarro J

更新日期：2012-12-01 00:00:00

abstract：:Parasitologic confirmation of cutaneous leishmaniasis is obligatory before chemotherapy can be considered. Direct microscopic examination of scrapings taken from indurated borders of ulcers has been routinely used as primary method of diagnosis. In this report we compared the sensitivity of examination of dermal scrap...

journal_title：Journal of clinical microbiology

authors： Ramírez JR,Agudelo S,Muskus C,Alzate JF,Berberich C,Barker D,Velez ID

更新日期：2000-10-01 00:00:00

abstract：:Approximately 15 to 35% of patients with a first episode of Clostridium difficile-associated diarrhea relapse within 2 months. Between 1994 and 1997, strains from 93 hospitalized patients with C. difficile recurrences were fingerprinted by using both serotyping and PCR-ribotyping. The results showed that 48.4% of clin...

journal_title：Journal of clinical microbiology

authors： Barbut F,Richard A,Hamadi K,Chomette V,Burghoffer B,Petit JC

更新日期：2000-06-01 00:00:00

abstract：:Nineteen reference and 156 clinical strains of the genus Nocardia belonging to 12 taxonomic groups were studied for restriction fragment length polymorphism (RFLP) by using an amplified 439-bp segment of the 65-kDa heat shock protein gene. Of 30 restriction endonucleases, digestion with MspI and then digestion with Bs...

journal_title：Journal of clinical microbiology

authors： Steingrube VA,Brown BA,Gibson JL,Wilson RW,Brown J,Blacklock Z,Jost K,Locke S,Ulrich RF,Wallace RJ Jr

更新日期：1995-12-01 00:00:00

abstract：:A slightly modified Moeller medium for amino acid decarboxylase and dihydrolase testing by the replicator method correlated 96 to 99% with the reference Moeller broth method. False positive results were not encountered in this study. ...

journal_title：Journal of clinical microbiology

authors： Jones RN,Fuchs PC,Sniderman S

更新日期：1976-01-01 00:00:00

abstract：:Fifteen common serotypes representative of group C(1) and C(2)Salmonella were characterized using a single set of phages. ...

journal_title：Journal of clinical microbiology

authors： Gershman M

更新日期：1976-02-01 00:00:00

abstract：:In a survey of bloodstream infection (BSI) isolates across the continental United States, 162 Candida albicans isolates were fingerprinted with the species-specific probe Ca3 and the patterns were analyzed for relatedness with a computer-assisted system. The results demonstrate that particular BSI strains are more hig...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Lockhart SR,Pujol C,Swails-Wenger JA,Messer SA,Edmond MB,Jones RN,Wenzel RP,Soll DR

更新日期：1998-06-01 00:00:00

abstract：:In total, 521 nonrespiratory specimens (91 urine, 30 gastric aspirate, 245 tissue, 113 pleural fluid, 19 cerebrospinal fluid [CSF], and 23 stool specimens) submitted to the German National Reference Laboratory for Mycobacteria (NRL) from May 2009 to August 2010 were comparatively investigated with the new molecular-ba...

journal_title：Journal of clinical microbiology

authors： Hillemann D,Rüsch-Gerdes S,Boehme C,Richter E

更新日期：2011-04-01 00:00:00

abstract：:A passive hemagglutination (PHA) assay for antibody to Pseudomonas aeruginosa exotoxin is described which utilizes chromic chloride-treated ovine erythrocytes coated with purified toxin. PHA antitoxin titers correlated well with those obtained by a cytotoxicity neutralization assay (r = 0.91, P less than 0.001), where...

journal_title：Journal of clinical microbiology

authors： Pollack M,Taylor NS

更新日期：1977-07-01 00:00:00

abstract：:Longitudinal data on the E6/E7 mRNA-based Aptima human papillomavirus (AHPV) assay exceeding three years in comparison to the gold standard Digene Hybrid Capture 2 (HC2) test are not available. We previously reported the cross-sectional data of the German AHPV Screening Trial (GAST) in which 10,040 women were recruite...

journal_title：Journal of clinical microbiology

authors： Iftner T,Neis KJ,Castanon A,Landy R,Holz B,Woll-Herrmann A,Iftner A,Staebler A,Wallwiener D,Hann von Weyhern C,Neis F,Haedicke-Jarboui J,Martus P,Brucker S,Henes M,Sasieni P

更新日期：2019-01-02 00:00:00

abstract：:The recently sequenced genome of Campylobacter jejuni RM1221 revealed the presence of three integrated bacteriophage-like elements. In this study, genes from the first element, a Mu-like bacteriophage, were amplified by PCR and used to probe pulsed-field gels of clinical C. jejuni strains obtained from a waterborne ou...

journal_title：Journal of clinical microbiology

authors： Barton C,Ng LK,Tyler SD,Clark CG

更新日期：2007-02-01 00:00:00

abstract：:In five separate fecal collections spanning three years, group A rotaviruses were detected by enzyme-linked immunosorbent assay in 35 (25%) of 142 specimens obtained from nondiarrheic, hospitalized neonates in Blantyre, Malawi. Molecular characterization of each strain identified, for the first time in neonates, a sho...

journal_title：Journal of clinical microbiology

authors： Cunliffe NA,Rogerson S,Dove W,Thindwa BD,Greensill J,Kirkwood CD,Broadhead RL,Hart CA

更新日期：2002-04-01 00:00:00

abstract：:Recently, we reported that the patterns of antibodies to Helicobacter pylori protein antigens in serum may be useful for screening patients at high risk for ulcers (P. Aucher et al., J. Clin. Microbiol. 36:931-936, 1998). Here we report the identification, by a combination of electrophoretic, immunochemical, and prote...

journal_title：Journal of clinical microbiology

authors： Atanassov C,Pezennec L,d'Alayer J,Grollier G,Picard B,Fauchère JL

更新日期：2002-02-01 00:00:00

abstract：:Epstein-Barr virus (EBV) infection is associated with a broad spectrum of disease. While quantification of EBV nucleic acid in the peripheral blood has been demonstrated to be useful for diagnosis and patient care, the optimal sample type and reporting format for such testing remain uncertain. Using quantitative real-...

journal_title：Journal of clinical microbiology

authors： Hakim H,Gibson C,Pan J,Srivastava K,Gu Z,Bankowski MJ,Hayden RT

更新日期：2007-07-01 00:00:00

abstract：:The analytical performance of the AIX1000 system, a fully automated and recently FDA-cleared rapid plasma reagin (RPR) system, was evaluated by comparison to a manual RPR test in a traditional syphilis testing algorithm. A total of 1,028 consecutive serum samples submitted for syphilis testing to the University of Nor...

journal_title：Journal of clinical microbiology

authors： Sanfilippo AM,Freeman K,Schmitz JL

更新日期：2018-07-26 00:00:00