Abstract:
:The recently sequenced genome of Campylobacter jejuni RM1221 revealed the presence of three integrated bacteriophage-like elements. In this study, genes from the first element, a Mu-like bacteriophage, were amplified by PCR and used to probe pulsed-field gels of clinical C. jejuni strains obtained from a waterborne outbreak (Ontario, Canada, 2000). These highly similar strains differed only by their pulsed-field gel electrophoresis (PFGE) patterns due to an apparent insertion or deletion of a 40-kb fragment. Bacteriophage probes hybridized to these different bands in Southern blot analysis, indicating that homologues of bacteriophage genes were present in the outbreak strains. Investigation of the bacteriophage insertion sites in these isolates suggested that bacteriophage acquisition, loss, or transposition was responsible for the PFGE pattern variation. The bacteriophage gene sequences were similar, but not identical, in the outbreak strains and RM1221, indicating that differences may exist between the bacteriophages.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Barton C,Ng LK,Tyler SD,Clark CGdoi
10.1128/JCM.01513-06subject
Has Abstractpub_date
2007-02-01 00:00:00pages
386-91issue
2eissn
0095-1137issn
1098-660Xpii
JCM.01513-06journal_volume
45pub_type
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