Abstract:
:The sensitive peroxidase-antiperoxidase (PXAPX) method individually and in conjunction with the Papanicolaou (PAP) stain was used to detect herpes simplex virus (HSV) in specimens from human female genitalia. Initial studies using a model system of HSV-1 or HSV-2-infected Vero cells established (i) acetone as the most effective fixative, (ii) optimal dilutions of preimmunization and anti-HSV serum for differentiation of infected from noninfected cells, (iii) optimal concentration of 3,3'-diaminobenzidine tetrahydrochloride and H2O2 for maximal staining of infected cells with minimal background reaction, and (iv) removal of endogenous peroxidase with absolute MeOH. These various parameters, once established, were utilized in the PXAPX or PXAPX-PAP on human specimens from the vulva or cervix. In these specimens, examined by standard light microscopy, PXAPX-positive cells were dark brown with a single nucleus or multiple nuclei. By coupling the PAP to the PXAPX, detailed nuclear observations of PXAPX-positive cells were possible and revealed nuclear changes consistent with HSV infection, including syncytium formation, chromatin condensation, and an occasional Cowdry type A inclusion. The PXAPX and PXAPX-PAP correlated (r = 0.742) over a period of 72 h with HSV isolation from these samples.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Pearson NS,Fleagle G,Docherty JJdoi
10.1128/JCM.10.5.737-746.1979subject
Has Abstractpub_date
1979-11-01 00:00:00pages
737-46issue
5eissn
0095-1137issn
1098-660Xjournal_volume
10pub_type
杂志文章abstract::A multiwell, dried antimicrobial agent susceptibility test system, Sceptor (BBL Microbiology Systems, Cockeysville, Md.), was tested. The system was compared directly with a reference microdilution method by using two collections of stock cultures and 305 fresh clinical isolates. Sceptor was found to be in agreement (...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.13.1.184-194.1981
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.4.648-653.1988
更新日期:1988-04-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.9.1611-1622.1987
更新日期:1987-09-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.26.6.1248-1249.1988
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.12.2874-2876.1989
更新日期:1989-12-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.10.2844-2846.1998
更新日期:1998-10-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:1986-02-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:2005-08-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:1997-02-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:1977-01-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.9.1955-1958.1991
更新日期:1991-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 临床试验,杂志文章
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更新日期:1996-07-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.4.1050-1053.1994
更新日期:1994-04-01 00:00:00