Evaluation of the Sceptor microdilution antibiotic susceptibility testing system: a collaborative investigation.

abstract：:We evaluated the performance and the cost of toxigenic culture using a commercial chromogenic medium (CDIF) for 538 stool specimens. Compared with real-time PCR, this method was found to detect an additional 9% of positive specimens and result in 61% reduction in material costs, with a trade-off increase in turnaround...

journal_title：Journal of clinical microbiology

authors： Luk S,To WK,Ng TK,Hui WT,Lee WK,Lau F,Ching AM

更新日期：2014-02-01 00:00:00

abstract：:Pasteurella species are part of the oral flora of cats and dogs. In humans, they are frequently found in infected animal bite wounds, but invasive infections are rare. This is the first report of prosthetic-valve endocarditis with a Pasteurella dagmatis-like species, which originated from the patient's cat. ...

journal_title：Journal of clinical microbiology

authors： Strahm C,Goldenberger D,Gutmann M,Kuhnert P,Graber P

更新日期：2012-08-01 00:00:00

abstract：:Here we describe the first reported case of Nocardia beijingensis infection in the United States, made rarer by its presence in an immunocompetent patient. ...

journal_title：Journal of clinical microbiology

authors： Crozier JA,Andhavarapu S,Brumble LM,Sher T

更新日期：2014-07-01 00:00:00

abstract：:Latex particle agglutination (LPA) testing for antigen to group B streptococcus (GBS) has been useful in the diagnosis of GBS sepsis in newborns. However, recent reports have demonstrated that the sensitivity of LPA assays may be as low as 27 to 54%. The purposes of the present study were to directly compare the abili...

journal_title：Journal of clinical microbiology

authors： Greenberg DN,Ascher DP,Yoder BA,Hensley DM,Heiman HS,Keith JF 3rd

更新日期：1995-01-01 00:00:00

abstract：:Infectious gastroenteritis is one of the most common diseases in young children. To clarify the infectious etiology of diarrhea in Danish children less than 5 years of age, we conducted a 2-year prospective case-control study. Stools from 424 children with diarrhea and 870 asymptomatic age-matched controls were examin...

journal_title：Journal of clinical microbiology

authors： Olesen B,Neimann J,Böttiger B,Ethelberg S,Schiellerup P,Jensen C,Helms M,Scheutz F,Olsen KE,Krogfelt K,Petersen E,Mølbak K,Gerner-Smidt P

更新日期：2005-08-01 00:00:00

abstract：:In a prospective study of 16 patients with Legionnaires disease confirmed by cultural isolation of Legionella pneumophila from the respiratory tract, 38% (6 of 16) had positive blood cultures. Daily subcultures were made onto buffered charcoal-yeast extract plates from 6B aerobic and 7C anaerobic BACTEC blood culture ...

journal_title：Journal of clinical microbiology

authors： Rihs JD,Yu VL,Zuravleff JJ,Goetz A,Muder RR

更新日期：1985-09-01 00:00:00

abstract：:Campylobacter species are the leading agents of bacterial gastroenteritis in developed countries. In this study 320 specimens of feces from patients with symptoms of acute gastroenteritis were cultured for Campylobacter species by direct plating on modified charcoal cefoperazone deoxycholate agar and by enrichment in ...

journal_title：Journal of clinical microbiology

authors： Maher M,Finnegan C,Collins E,Ward B,Carroll C,Cormican M

更新日期：2003-07-01 00:00:00

abstract：:Two brands of thiosulfate citrate bile salts sucrose agar and Monsur taurocholate tellurite gelatin (TTG) agar were compared with two newly developed media, sucrose tellurite teepol agar and Vibrio parahaemolyticus agar for isolation and identification of Vibrio cholerae. The thiosulfate citrate bile salts sucrose and...

journal_title：Journal of clinical microbiology

authors： Morris GK,Merson MH,Huq I,Kibrya AK,Black R

更新日期：1979-01-01 00:00:00

abstract：:The ERA strain of rabies virus was adapted to growth on monolayers of a porcine kidney cell line (PK-2A). A reproducible plaque assay system was subsequently developed, which appears to be satisfactory for conducting plaque neutralization tests. ...

journal_title：Journal of clinical microbiology

authors： Strating A,Mitzel JR,Slater EA

更新日期：1976-08-01 00:00:00

abstract：:Chromosomal DNAs of selected Bacteroides organisms whose relatedness had been previously determined by "conventional" filter-annealing studies (J. L. Johnson, Int. J. Syst. Bacteriol. 28:245, 1978) were further analyzed by restriction endonuclease analysis coupled with the Southern hybridization procedure (E. M. South...

journal_title：Journal of clinical microbiology

authors： Bradbury WC,Murray RG,Mancini C,Morris VL

更新日期：1985-01-01 00:00:00

abstract：:In a multicenter study, 113 blood samples from 19 organ transplant patients were analyzed for cytomegalovirus by the pp65 antigenemia assay and a quantitative DNA hybridization assay. Overall, there was 84% agreement among the results obtained by the two tests. Fifteen of 16 episodes of active infection were detected ...

journal_title：Journal of clinical microbiology

authors： Bossart W,Bienz K,Wunderli W

更新日期：1997-12-01 00:00:00

abstract：:Serratia ficaria was first described in 1979 as part of the fig tree ecosystem (P.A.D. Grimont, F. Grimont, and M. P. Starr, Curr. Microbiol. 2:277-282, 1979). Since then, it has been isolated from clinical specimens from a few human patients (C. Bollet, J. Freney, P. de Micco, F. Grimont, and P.A.D. Grimont, Méd. Mal...

journal_title：Journal of clinical microbiology

authors： Darbas H,Jean-Pierre H,Paillisson J

更新日期：1994-09-01 00:00:00

abstract：:This study evaluated the feasibility of a molecular strategy based on identification of Mycobacterium tuberculosis by IS6110 PCR or Cobas Amplicor PCR, and rpoB PCR-DNA sequencing of the 81-bp rifampin resistance determining region (RRDR) for direct detection of rifampin resistance in respiratory specimens. A collecti...

journal_title：Journal of clinical microbiology

authors： Yam WC,Tam CM,Leung CC,Tong HL,Chan KH,Leung ET,Wong KC,Yew WW,Seto WH,Yuen KY,Ho PL

更新日期：2004-10-01 00:00:00

abstract：:During an outbreak of diarrheal illness among residents of a trailer park in rural Vermont, 37 (30%) of 122 residents met the case definition of outbreak-related giardiasis. Convalescent-phase sera from 24 residents and 20 nonresident control subjects were tested by enzyme-linked immunosorbent assay for immunoglobulin...

journal_title：Journal of clinical microbiology

authors： Birkhead G,Janoff EN,Vogt RL,Smith PD

更新日期：1989-08-01 00:00:00

abstract：:We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with r...

journal_title：Journal of clinical microbiology

authors： Cunningham SA,Noorie T,Meunier D,Woodford N,Patel R

更新日期：2013-04-01 00:00:00

abstract：:A total of 322 single and paired serum samples from children (newborn to 10 years old), young adults (18 to 20 years old), pregnant women and their cord serum samples, and elderly people (more than 70 years old) was tested for antibodies to enteric adenovirus types 40 and 41 by neutralization test. Serum samples were ...

journal_title：Journal of clinical microbiology

authors： Shinozaki T,Araki K,Ushijima H,Fujii R

更新日期：1987-09-01 00:00:00

abstract：:Electrophoretic karyotypes of atypical isolates of Candida albicans, e.g., strains that were germ tube negative, failed to express proteinase activity, demonstrated low virulence for mice, formed hyperchlamydoconidia, produced hyperhyphae, or were sucrose negative (including the type strain of Candida stellatoidea), w...

journal_title：Journal of clinical microbiology

authors： Mahrous M,Lott TJ,Meyer SA,Sawant AD,Ahearn DG

更新日期：1990-05-01 00:00:00

abstract：:A modified Elek test and an immuno-halo test were developed for detection of the thermostable direct hemolysin produced by Kanagawa phenomenon-positive strains of Vibrio parahaemolyticus. The sensitivity of the modified Elek test was almost the same as that of the Kanagawa phenomenon on Wagatsuma medium, and the resul...

journal_title：Journal of clinical microbiology

authors： Honda T,Chearskul S,Takeda Y,Miwatani T

更新日期：1980-06-01 00:00:00

abstract：:An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. Th...

journal_title：Journal of clinical microbiology

authors： Wallis C,Melnick JL,Longoria CJ

更新日期：1981-09-01 00:00:00

abstract：:A gag-based molecular beacon assay utilizing real-time nucleic acid sequence-based amplification technology has been developed to differentiate between the two genetic subclusters of human immunodeficiency virus type 1 (HIV-1) subtype C (C and C') circulating in Ethiopia. Of 41 samples, 36 could be classified as C or ...

journal_title：Journal of clinical microbiology

authors： Ayele W,Pollakis G,Abebe A,Fisseha B,Tegbaru B,Tesfaye G,Mengistu Y,Wolday D,van Gemen B,Goudsmit J,Dorigo-Zetsma W,de Baar MP

更新日期：2004-04-01 00:00:00

abstract：:Multidrug-resistant (MDR) Klebsiella pneumoniae is one of the most important causes of nosocomial infections worldwide. After the spread of strains resistant to beta-lactams at the end of the previous century, the diffusion of isolates resistant to carbapenems and colistin is now reducing treatment options and the con...

journal_title：Journal of clinical microbiology

authors： Onori R,Gaiarsa S,Comandatore F,Pongolini S,Brisse S,Colombo A,Cassani G,Marone P,Grossi P,Minoja G,Bandi C,Sassera D,Toniolo A

更新日期：2015-09-01 00:00:00

abstract：:A total of 563 specimens (234 dermal and 329 genital swabs) from patients suspected of having herpes simplex virus (HSV) infections were processed using two different extraction methods (the MagNA Pure LC system and the swab extraction tube system [SETS]); HSV DNA was amplified by LightCycler PCR. HSV DNA was detected...

journal_title：Journal of clinical microbiology

authors： Issa NC,Espy MJ,Uhl JR,Harmsen WS,Mandrekar JN,Gullerud RE,Davis MD,Smith TF

更新日期：2005-03-01 00:00:00

abstract：:The commercially available diagnostic tests for syphilis are mostly based on the use of extracted antigens of Treponema pallidum. Pronounced cross-reactivities with other spirochete antigens are often reported. The aim of this study was to validate a novel multiparametric assay (the assay performed with the kit) INNO-...

journal_title：Journal of clinical microbiology

authors： Ebel A,Vanneste L,Cardinaels M,Sablon E,Samson I,De Bosschere K,Hulstaert F,Zrein M

更新日期：2000-01-01 00:00:00

abstract：:Formalin-fixed, purified reticulate bodies (RB) of Chlamydia trachomatis immunotype C/TW-3/OT were used as a serological test antigen in the microimmunofluorescence test. The sensitivity and specificity of the RB antigen were compared to elementary bodies (EB) used as antigens in the detection of C. trachomatis antibo...

journal_title：Journal of clinical microbiology

authors： Yong EC,Chinn JS,Caldwell HD,Kuo CC

更新日期：1979-09-01 00:00:00

abstract：:This is the first report in Israel of a cluster of cases of diarrhea associated with the isolation of Escherichia coli O157 in stool samples. E. coli O157:NM (nonmotile), producing verotoxins 1 and 2 was isolated from the stool samples of three infants less than 1 year of age with diarrhea and from a fourth asymptomat...

journal_title：Journal of clinical microbiology

authors： Lerman Y,Cohen D,Gluck A,Ohad E,Sechter I

更新日期：1992-02-01 00:00:00

abstract：:While high-copy-number IS6110-based restriction fragment length polymorphism (HCN-RFLP) is the gold standard for typing most Mycobacterium tuberculosis strains, the time taken for culturing and low throughput make it impractical for large-scale prospective typing of large numbers of isolates. The development of a new ...

journal_title：Journal of clinical microbiology

authors： Gopaul KK,Brown TJ,Gibson AL,Yates MD,Drobniewski FA

更新日期：2006-07-01 00:00:00

abstract：:Pulsed-field gel electrophoresis revealed that multiple consecutive isolates of Legionella pneumophila from two cardiac transplant patients remained genomically stable, despite exposure to host defenses and antimicrobial agents. ...

journal_title：Journal of clinical microbiology

authors： Marrie TJ,Johnson WM,Tyler SD,Bezanson GS,Burbridge S

更新日期：1994-12-01 00:00:00

abstract：:Between November 1983 and January 1984, 13 cases of Candida parapsilosis endophthalmitis occurred in Florida, Georgia, and Tennessee in patients who had had an intraocular lens implantation (IOLI) or cataract extraction with an IOLI. This outbreak followed the introduction in July 1983 of a new brand of balanced salt ...

journal_title：Journal of clinical microbiology

authors： McCray E,Rampell N,Solomon SL,Bond WW,Martone WJ,O'Day D

更新日期：1986-10-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the titration of antibodies in human sera to influenza virus neuraminidase, employing partially purified N1 neuraminidase. Specificity of the test was demonstrated, and the test was more sensitive than either the conventional neuraminidase inhibition or plaque siz...

journal_title：Journal of clinical microbiology

authors： Khan MW,Gallagher M,Bucher D,Cerini CP,Kilbourne ED

更新日期：1982-07-01 00:00:00

abstract：:The aims of this study were to compare a novel PCR-based Chlamydia trachomatis detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available C. trachomatis detection Hybrid Capture 2 (HC2) assay and to investigate the C. trachomatis serovar distribution among young women in a rural Costa Rican s...

journal_title：Journal of clinical microbiology

authors： Quint K,Porras C,Safaeian M,González P,Hildesheim A,Quint W,van Doorn LJ,Silva S,Melchers W,Schiffman M,Rodríguez AC,Wacholder S,Freer E,Cortes B,Herrero R,Costa Rican Vaccine Trial Group.

更新日期：2007-12-01 00:00:00