Immunological methods for detection of Kanagawa phenomenon of Vibrio parahaemolyticus.

abstract：:A dot immunobinding assay that uses inactivated antigen for the detection of rabies viral antibodies was compared with the rapid fluorescent focus inhibition test. Results of testing pre- and postvaccination sera from humans (n = 33) and canines (n = 22) were identical for both tests. Endpoint titers of positive sera ...

journal_title：Journal of clinical microbiology

authors： Heberling RL,Kalter SS,Smith JS,Hildebrand DG

更新日期：1987-07-01 00:00:00

abstract：:Many commercially available media for cultivation of mycobacteria have failed to support the growth of these organisms. This is especially true of media prepared with albumin-containing enrichments. Earlier, we developed a method for rapid identification of good albumin enrichments for agar-based media used to test th...

journal_title：Journal of clinical microbiology

authors： Butler WR,Warren NG,Kubica GP,Kilburn JO

更新日期：1990-05-01 00:00:00

abstract：:Diarrheal diseases are highly prevalent in Bangladesh. However, the relative contribution of diarrheagenic Escherichia coli organisms--those that are enterotoxigenic (ETEC), enteropathogenic (EPEC), enteroinvasive, enterohemorrhagic, enteroaggregative, and diffuse adherent--to diarrhea in Bangladeshi populations is no...

journal_title：Journal of clinical microbiology

authors： Albert MJ,Faruque SM,Faruque AS,Neogi PK,Ansaruzzaman M,Bhuiyan NA,Alam K,Akbar MS

更新日期：1995-04-01 00:00:00

abstract：:Screening of blood for human T-cell lymphotropic virus type 1 and type 2 (HTLV-1 and -2, respectively) is important to diagnose and prevent infection and ensure the safety of blood supplies. The Elecsys HTLV-I/II assay is a newly developed, electrochemiluminescence screening assay for the detection of HTLV-1/2 infecti...

journal_title：Journal of clinical microbiology

authors： Laperche S,Sauleda S,Piron M,Mühlbacher A,Schennach H,Schottstedt V,Queirós L,Uno N,Yanagihara K,Imdahl R,Hey A,Klinkicht M,Melchior W,Muench P,Watanabe T

更新日期：2017-07-01 00:00:00

abstract：:The utility of the GeneXpert MTB/RIF (Xpert) assay for detection of Mycobacterium tuberculosis in sputum samples has been extensively studied. However, the performance of the Xpert assay as applied to other readily accessible body fluids such as exhaled breath condensate (EBC), saliva, urine, and blood has not been es...

journal_title：Journal of clinical microbiology

authors： Shenai S,Amisano D,Ronacher K,Kriel M,Banada PP,Song T,Lee M,Joh JS,Winter J,Thayer R,Via LE,Kim S,Barry CE 3rd,Walzl G,Alland D

更新日期：2013-12-01 00:00:00

abstract：:A cytopathic astrovirus was isolated from pigs with acute diarrhea in an established cell line that was derived from porcine embryonic kidneys with the aid of trypsin. The virus showed a distinct cytopathic effect characterized by an enlargement of cells and the appearance of fine granules in the cytoplasm. Porcine as...

journal_title：Journal of clinical microbiology

authors： Shimizu M,Shirai J,Narita M,Yamane T

更新日期：1990-02-01 00:00:00

abstract：:The use of immunoglobulin M (IgM) antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) serves as a valuable tool for the diagnosis of acute flaviviral infections, since IgM antibody titers are detectable early, peak at about 2 weeks postinfection, and subsequently decline to lower levels over the next few mo...

journal_title：Journal of clinical microbiology

authors： Holmes DA,Purdy DE,Chao DY,Noga AJ,Chang GJ

更新日期：2005-07-01 00:00:00

abstract：:A 2000-2001 U.S. Haemophilus influenzae surveillance study (n = 1,434) detected nine (0.6%) beta-lactamase-negative and ampicillin-resistant (BLNAR) isolates collected from two different hospitals. The MICs of ampicillin for all nine isolates were 4 microg/ml, with results being reproducible; and all nine isolates wer...

journal_title：Journal of clinical microbiology

authors： Karlowsky JA,Critchley IA,Blosser-Middleton RS,Karginova EA,Jones ME,Thornsberry C,Sahm DF

更新日期：2002-03-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the detection of circulating free and complexed staphylococcal antigens in various clinical categories of staphylococcal infections. Circulating immune complexes were studied by the polyethylene glycol precipitation method. Circulating immune complexes and staphyl...

journal_title：Journal of clinical microbiology

authors： Lentino JR,Rytel MW

更新日期：1982-12-01 00:00:00

abstract：:A qualitative multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was developed. Furthermore, a separate assay for the recognition of hepatitis A virus was established to complement the test pattern so that all ...

journal_title：Journal of clinical microbiology

authors： Jokela P,Joki-Korpela P,Maaronen M,Glumoff V,Hyypiä T

更新日期：2005-03-01 00:00:00

abstract：:Recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (AHI); however, the cost of nucleic acid amplification testing (NAAT) makes individual testing of at-risk individuals prohibitively expensive in many settings. Pooled NAAT (or group testi...

journal_title：Journal of clinical microbiology

authors： Westreich DJ,Hudgens MG,Fiscus SA,Pilcher CD

更新日期：2008-05-01 00:00:00

abstract：:It has been shown that some, but not all, Escherichia coli strains isolated from urine adhere, in vitro, to the surface of uroepithelial or vaginal cells. In the present study, 212 strains, isolated from urine of 212 infected patients, were tested for adhesion by using an in vitro human cell line assay. A variable deg...

journal_title：Journal of clinical microbiology

authors： Chabanon G,Hartley CL,Richmond MH

更新日期：1979-10-01 00:00:00

abstract：:Results of a 2003 survey carried out in Italy to evaluate the prevalence of extended-spectrum beta-lactamase (ESBL)-producing enterobacteria are presented. Eleven Italian Microbiology Laboratories investigated 9,076 consecutive nonreplicate isolates (inpatients, 6,850; outpatients, 2,226). ESBL screening was performed...

journal_title：Journal of clinical microbiology

authors： Luzzaro F,Mezzatesta M,Mugnaioli C,Perilli M,Stefani S,Amicosante G,Rossolini GM,Toniolo A

更新日期：2006-05-01 00:00:00

abstract：:The mainstay of laboratory diagnosis for Lyme disease is two-tiered serological testing, in which a reactive first-tier enzyme-linked immunosorbent assay (ELISA) or an immunofluorescence assay is supplemented by separate IgM and IgG immunoblots. Recent data suggest that the C6 ELISA can be substituted for immunoblots ...

journal_title：Journal of clinical microbiology

authors： Wormser GP,Levin A,Soman S,Adenikinju O,Longo MV,Branda JA

更新日期：2013-12-01 00:00:00

abstract：:In titrating serum immunoglobulin G antibody to viruses by enzyme-linked immunosorbent assay, we used two rows of wells for serial twofold dilutions of the serum; in one row, a low concentration of a protein denaturant, 0.5 or 1.0 M guanidine hydrochloride, was added to the diluent so that the binding of low-avidity a...

journal_title：Journal of clinical microbiology

authors： Inouye S,Hasegawa A,Matsuno S,Katow S

更新日期：1984-09-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection typically leads to antibody response within weeks after primary infection. Here, we describe the case of a child infected with HCV by mother-to-child transmission who remained persistently seronegative despite the presence of high levels of circulating HCV RNA. ...

journal_title：Journal of clinical microbiology

authors： Larouche A,Gaëtan G,El-Bilali N,Quesnel-Vallières M,Martin SR,Alvarez F,Shoukry NH,Soudeyns H

更新日期：2012-07-01 00:00:00

abstract：:We have studied 221 adults drawn from an impoverished urban population with high human immunodeficiency virus (HIV) seroprevalence (35%) to determine the prevalence of gastroduodenal pathology and its relationship to serological markers of Helicobacter pylori virulence proteins and other potential environmental and im...

journal_title：Journal of clinical microbiology

authors： Fernando N,Holton J,Zulu I,Vaira D,Mwaba P,Kelly P

更新日期：2001-04-01 00:00:00

abstract：:Between 1999 and 2002, 496 invasive group A streptococcal (GAS) isolates from clinical microbiological departments in Denmark and subsequently 487 (98%) questionnaires from the clinicians treating the patients were received as part of a national surveillance. emm types and streptococcal superantigen (SAg) genes were d...

journal_title：Journal of clinical microbiology

authors： Ekelund K,Skinhøj P,Madsen J,Konradsen HB

更新日期：2005-04-01 00:00:00

abstract：:A monoclonal antibody-based coagglutination test directly detected Vibrio cholerae O139 synonym Bengal in 83 of 120 watery diarrheal stool specimens; on culture, 90 samples were positive. Thus, with 92% sensitivity, 100% specificity, and 100% positive and 95% negative predictive values, the coagglutination test is a u...

journal_title：Journal of clinical microbiology

authors： Qadri F,Chowdhury A,Hossain J,Chowdhury K,Azim T,Shimada T,Islam KM,Sack RB,Albert MJ

更新日期：1994-06-01 00:00:00

abstract：:Although two phenotypes of the opportunistic pathogen Propionibacterium acnes (types I and II) have been described, epidemiological investigations of their roles in different infections have not been widely reported. Using immunofluorescence microscopy with monoclonal antibodies (MAbs) QUBPa1 and QUBPa2, specific for ...

journal_title：Journal of clinical microbiology

authors： McDowell A,Valanne S,Ramage G,Tunney MM,Glenn JV,McLorinan GC,Bhatia A,Maisonneuve JF,Lodes M,Persing DH,Patrick S

更新日期：2005-01-01 00:00:00

abstract：:The aim of this study was to determine if gastroenteric viruses were present on surfaces and equipment in a pediatric primary immunodeficiency unit (PPIU) by environmental sampling using swabs and subsequent nucleic acid extraction and reverse transcriptase PCR assays. A PPIU was chosen, and 11 swabs were taken at the...

journal_title：Journal of clinical microbiology

authors： Gallimore CI,Taylor C,Gennery AR,Cant AJ,Galloway A,Iturriza-Gomara M,Gray JJ

更新日期：2006-02-01 00:00:00

abstract：:Three susceptibility testing procedures were compared to determine fluconazole, itraconazole, and ketoconazole MICs against 47 Candida albicans strains isolated sequentially from the oral cavities of five AIDS patients undergoing azole therapy. They included the broth microdilution method (BM), performed according to ...

journal_title：Journal of clinical microbiology

authors： Tortorano AM,Viviani MA,Barchiesi F,Arzeni D,Rigoni AL,Cogliati M,Compagnucci P,Scalise G

更新日期：1998-06-01 00:00:00

abstract：:We developed a novel gelatin particle agglutination test (MLPA) for the serodiagnosis of leprosy; this test is especially useful for clinical practice and epidemiological surveys of leprosy in countries in which the disease is endemic. The antigen used in the test is the chemically synthesized trisaccharide moiety of ...

journal_title：Journal of clinical microbiology

authors： Izumi S,Fujiwara T,Ikeda M,Nishimura Y,Sugiyama K,Kawatsu K

更新日期：1990-03-01 00:00:00

abstract：:The development of a rhinovirus (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5'-noncoding region, which is chosen for primer design in most RT-PCR assays. We have developed a sensitive, rapid, and RV-spec...

journal_title：Journal of clinical microbiology

authors： Steininger C,Aberle SW,Popow-Kraupp T

更新日期：2001-01-01 00:00:00

abstract：:A TaqMan real-time PCR assay, the COBAS TaqMan human immunodeficiency virus (HIV) (HPS-CTMHIV) PCR assay, recently developed for the quantification of HIV type 1 RNA in plasma, was evaluated in comparison with the licensed COBAS AMPLICOR HIV-1 MONITOR (CAHIM) assay. In this study, we have analyzed the tests' sensitivi...

journal_title：Journal of clinical microbiology

authors： Perrin L,Pawlotsky JM,Bouvier-Alias M,Sarrazin C,Zeuzem S,Colucci G

更新日期：2006-12-01 00:00:00

abstract：:DNA fingerprint patterns of 156 Cryptococcus neoformans isolates (26 AIDS patients, 46 non-AIDS patients, and 40 environmental sources) from both varieties (126 C. neoformans var. neoformans and 30 C. neoformans var. gattii isolates) and from seven countries were analyzed by using the DNA probe UT-4p. Nine and twelve ...

journal_title：Journal of clinical microbiology

authors： Varma A,Swinne D,Staib F,Bennett JE,Kwon-Chung KJ

更新日期：1995-07-01 00:00:00

abstract：:Haemophilus influenzae type e biotype 4 was isolated from a single antemortem blood culture obtained from a 60-year-old, white male with abdominal carcinomatosis. ...

journal_title：Journal of clinical microbiology

authors： Cawthern TH,Marraro RV,McCleskey FK

更新日期：1978-01-01 00:00:00

abstract：:The identification and elimination of persistently infected (PI) cattle are the most effective measures for controlling bovine pestiviruses, including bovine viral diarrhea virus (BVDV) and the emerging HoBi-like viruses. Here, colostrum-deprived calves persistently infected with HoBi-like pestivirus (HoBi-like PI cal...

journal_title：Journal of clinical microbiology

authors： Bauermann FV,Falkenberg SM,Vander Ley B,Decaro N,Brodersen BW,Harmon A,Hessman B,Flores EF,Ridpath JF

更新日期：2014-11-01 00:00:00

abstract：:The development and application of a double-label immunofluorescence method which has the potential to screen for single or dual infections from any site, in single shell vial cultures, is described. In this study, a total of 1,141 ocular specimens were inoculated in shell vials, centrifuged at 15,000 X g for 1 h, inc...

journal_title：Journal of clinical microbiology

authors： Walpita P,Darougar S

更新日期：1989-07-01 00:00:00

abstract：:We report on the development of a fully automated real-time PCR assay for the quantitative detection of hepatitis B virus (HBV) DNA in plasma with EDTA (EDTA plasma). The MagNA Pure LC instrument was used for automated DNA purification and automated preparation of PCR mixtures. Real-time PCR was performed on the Light...

journal_title：Journal of clinical microbiology

authors： Leb V,Stöcher M,Valentine-Thon E,Hölzl G,Kessler H,Stekel H,Berg J

更新日期：2004-02-01 00:00:00