Genomic stability of Legionella pneumophila isolates recovered from two cardiac transplant patients with nosocomial Legionnaires' disease.

abstract：:Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. The available rapid detection methods either lack sensitivity or require complex laboratory manipulation. This study describes a rapid, sensitive detection method that can be easily applied to routine diagnosi...

journal_title：Journal of clinical microbiology

authors： van Elden LJ,Nijhuis M,Schipper P,Schuurman R,van Loon AM

更新日期：2001-01-01 00:00:00

abstract：:A recombinant protein (p41-G) of an antigenic region of flagellin was used in a standard and amplified enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Borrelia burgdorferi, the causative agent of Lyme borreliosis. Comparable sensitivities (88 to 94%) were noted when sera from 17 persons who had eryth...

journal_title：Journal of clinical microbiology

authors： Magnarelli LA,Fikrig E,Berland R,Anderson JF,Flavell RA

更新日期：1992-12-01 00:00:00

abstract：:The nasopharyngeal Haemophilus influenzae flora of healthy children in a day care center was analyzed by repeated sampling during 4 winter months. The average carrier rate was 39%, but 74% of the children became colonized at some time during the study. The H. influenzae isolates were identified by capsular type, bioty...

journal_title：Journal of clinical microbiology

authors： Trottier S,Stenberg K,Svanborg-Edén C

更新日期：1989-10-01 00:00:00

abstract：:Human metapneumovirus (HMPV) was the unique viral pathogen detected by a real-time reverse transcriptase PCR (RT-PCR) assay in 6 (6.4%) of 94 consecutive French children hospitalized for acute bronchiolitis from September 2001 to June 2002. This virus was identified as the third etiological cause of bronchiolitis, aft...

journal_title：Journal of clinical microbiology

authors： Bouscambert-Duchamp M,Lina B,Trompette A,Moret H,Motte J,Andréoletti L

更新日期：2005-03-01 00:00:00

abstract：:Botulism developed in a patient following surgical repair of an open radial fracture. Symptoms resolved after treatment with antitoxin and antibiotics, and hardware excision was deferred. Subsequent osteomyelitis necessitated hardware exchange, and wound cultures grew Clostridium argentinense. This case highlights the...

journal_title：Journal of clinical microbiology

authors： Taylor SM,Wolfe CR,Dixon TC,Ruch DS,Cox GM

更新日期：2010-02-01 00:00:00

abstract：:A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation of M. tuberculosis, Mycobacterium bovis, M. bovis BCG, and Mycobacterium africanum subtypes I and II. In addition, this approach was incorporated...

journal_title：Journal of clinical microbiology

authors： Yeboah-Manu D,Yates MD,Wilson SM

更新日期：2001-11-01 00:00:00

abstract：:We report an outbreak of Saccharomyces cerevisiae subtype boulardii fungemia among three intensive care unit roommates of patients receiving lyophilized preparations of this fungus. The fungemia was probably due to central venous catheter contamination and resolved after fluconazole treatment. The need for stringent a...

journal_title：Journal of clinical microbiology

authors： Cassone M,Serra P,Mondello F,Girolamo A,Scafetti S,Pistella E,Venditti M

更新日期：2003-11-01 00:00:00

abstract：:Quantitative PCR on nasopharyngeal aspirate (NPA) can achieve high sensitivity and specificity in diagnosing Pneumocystis pneumonia (PCP) compared to microscopic examination of bronchoscopic specimens in a population with low HIV prevalence. Since NPA is a minimally invasive procedure, it is ideal as a screening test ...

journal_title：Journal of clinical microbiology

authors： To KK,Wong SC,Xu T,Poon RW,Mok KY,Chan JF,Cheng VC,Chan KH,Hung IF,Yuen KY

更新日期：2013-05-01 00:00:00

abstract：:G3 rotaviruses have been reported rarely in cattle, and none have been characterized. We report the first genomic characterization of a bovine G3 rotavirus, CP-1, which had been biologically characterized in vivo and shown to cause age-independent diarrhea. CP-1 was a G3 rotavirus as its VP7 had 92 to 96% deduced amin...

journal_title：Journal of clinical microbiology

authors： El-Attar L,Dhaliwal W,Iturriza-Gómara M,Bridger JC

更新日期：2002-03-01 00:00:00

abstract：:Fingerprinting by randomly amplified polymorphic DNA (RAPD) analysis was used to differentiate Scedosporium prolificans isolates. A total of 59 arbitrary primers were screened with six unrelated S. prolificans isolates, and a panel of 12 primers was selected. The 12 primers were then used to detect DNA polymorphisms a...

journal_title：Journal of clinical microbiology

authors： San Millán R,Quindós G,Garaizar J,Salesa R,Guarro J,Pontón J

更新日期：1997-09-01 00:00:00

abstract：:Classifications of the Campylobacter fetus subspecies fetus and venerealis were first described in 1959 and were based on the source of isolation (intestinal versus genital) and the ability of the strains to proliferate in the genital tract of cows. Two phenotypic assays (1% glycine tolerance and H2S production) were ...

journal_title：Journal of clinical microbiology

authors： van der Graaf-van Bloois L,Miller WG,Yee E,Rijnsburger M,Wagenaar JA,Duim B

更新日期：2014-12-01 00:00:00

abstract：:Although TT virus (TTV) was isolated from a cryptogenic posttransfusion hepatitis patient, its pathogenic role remains unclear. It has been reported that the majority of the healthy population is infected with TTV. To elucidate the differences between TTV infection in patients with liver diseases and TTV infection in ...

journal_title：Journal of clinical microbiology

authors： Kato T,Mizokami M,Mukaide M,Orito E,Ohno T,Nakano T,Tanaka Y,Kato H,Sugauchi F,Ueda R,Hirashima N,Shimamatsu K,Kage M,Kojiro M

更新日期：2000-01-01 00:00:00

abstract：:We have shown that a modification of the color-coded antigen developed by Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974) can be used as a substitute for other nontreponemal antigens used in screening tests for syphilis. The antigen is based on the Venereal Disease Research Laboratory antigen, with EDTA, chol...

journal_title：Journal of clinical microbiology

authors： Pettit DE,Larsen SA,Harbec PS,Feeley JC,Parham CE,Cruce DD,Hambie EA,Perryman MW

更新日期：1983-11-01 00:00:00

abstract：:The diagnosis of congenital syphilis in apparently healthy infants continues to be problematic. Immunoglobulin M antibodies specific for a subset of Treponema pallidum antigens have been detected by Western blotting (immunoblotting). In the present study we investigated the sensitivity and specificity of this method. ...

journal_title：Journal of clinical microbiology

authors： Meyer MP,Eddy T,Baughn RE

更新日期：1994-03-01 00:00:00

abstract：:Bacteriuria due to Salmonella typhi usually occurs following recent typhoid fever or in chronic carrier states. Data from 18 patients with S. typhi bacteriuria, seen during 5 years, were analyzed. Fourteen patients had localized urinary tract infection due to S. typhi. Four others had bacteriuria, probably associated ...

journal_title：Journal of clinical microbiology

authors： Mathai E,John TJ,Rani M,Mathai D,Chacko N,Nath V,Cherian AM

更新日期：1995-07-01 00:00:00

abstract：:Sequential specimens from nine allograft recipients were examined by using a variety of methods to detect primary cytomegalovirus (CMV) infection as rapidly as possible posttransplantation. Sera were examined for immunoglobulin G (IgG) and IgM antibodies by immunoblotting, enzyme immunoassay, and immunofluorescence an...

journal_title：Journal of clinical microbiology

authors： Miller H,McCulloch B,Landini MP,Rossier E

更新日期：1989-12-01 00:00:00

abstract：:Detection of Staphylococcus aureus isolates with intermediate vancomycin susceptibility (VISA) and heteroresistance (hVISA) remains problematic. The population analysis profile/area under the curve (PAP/AUC) is the gold standard but is cumbersome. We compared the performance of two Etest screening methods (macromethod...

journal_title：Journal of clinical microbiology

authors： Riederer K,Shemes S,Chase P,Musta A,Mar A,Khatib R

更新日期：2011-06-01 00:00:00

abstract：:The accessory gene regulator (agr) is a central system that controls the expression of Staphylococcus aureus virulence factors. In this study, the distribution of agr alleles, defined by agr restriction fragment length polymorphism within agr interference groups, among S. aureus isolates from bovine and human origin w...

journal_title：Journal of clinical microbiology

authors： Gilot P,van Leeuwen W

更新日期：2004-03-01 00:00:00

abstract：:The IDI-MRSA assay (Infectio Diagnostic, Inc., Sainte-Foy, Quebec, Canada) with the Smart Cycler II rapid DNA amplification system (Cepheid, Sunnyvale, CA) appears to be sensitive and specific for the rapid detection of nasal colonization by methicillin-resistant Staphylococcus aureus (MRSA). We assessed the sensitivi...

journal_title：Journal of clinical microbiology

authors： Bishop EJ,Grabsch EA,Ballard SA,Mayall B,Xie S,Martin R,Grayson ML

更新日期：2006-08-01 00:00:00

abstract：:Saliva from subjects with amebic liver abscess (ALA), acute amebic colitis, asymptomatic infection with Entamoeba histolytica or Entamoeba dispar, and uninfected controls was tested by enzyme-linked immunosorbent assay (ELISA) for the presence of E. histolytica galactose-inhibitable lectin antigen and salivary immunog...

journal_title：Journal of clinical microbiology

authors： Abd-Alla MD,Jackson TF,Reddy S,Ravdin JI

更新日期：2000-06-01 00:00:00

abstract：:The development of a specimen collection and transport medium outfit for the rapid laboratory diagnosis of whoping cough is described. The transport medium consisted of a semisolid agar containing charcoal, cephalexin, and defibrinated horse blood. It was also found to be an excellent enrichment medium for the selecti...

journal_title：Journal of clinical microbiology

authors： Regan J,Lowe F

更新日期：1977-09-01 00:00:00

abstract：:Monoclonal antibodies reactive with three different viral polypeptides were evaluated singly and in combination as the capture antibody(s) in an enzyme-linked immunosorbent assay system for the detection of bovine enteric coronavirus. Similar levels of sensitivity were found for all combinations tested. A sensitive, h...

journal_title：Journal of clinical microbiology

authors： Crouch CF,Raybould TJ,Acres SD

更新日期：1984-03-01 00:00:00

abstract：:It has been shown by analysis with monoclonal and polyclonal antibodies that outer surface protein C (OspC) of Borrelia burgdorferi sensu lato is highly heterogeneous. To determine if the heterogeneity has a genetic basis, the genes of 18 different B. burgdorferi sensu lato strains have been amplified by PCR, cloned, ...

journal_title：Journal of clinical microbiology

authors： Jauris-Heipke S,Liegl G,Preac-Mursic V,Rössler D,Schwab E,Soutschek E,Will G,Wilske B

更新日期：1995-07-01 00:00:00

abstract：:To facilitate the routine identification of salmonellae and detailed studies of their lipopolysaccharides, we raised murine monoclonal antibodies against these organisms. We raised an immunoglobulin G1 antibody, MO2, which is specific for factor O2. By immunoblotting following sodium dodecyl sulfate-polyacrylamide gel...

journal_title：Journal of clinical microbiology

authors： Luk MC,Tsang RS,Ng MH

更新日期：1987-11-01 00:00:00

abstract：:A rapid phenotypic screening method for herpes simplex virus (HSV) and varicella-zoster virus (VZV) thymidine kinase (TK) genes was developed for monitoring acyclovir-resistant viruses. This method determines the biochemical phenotype of the TK polypeptide, which is synthesized in vitro from viral DNA using a procedur...

journal_title：Journal of clinical microbiology

authors： Suzutani T,Saijo M,Nagamine M,Ogasawara M,Azuma M

更新日期：2000-05-01 00:00:00

abstract：:Comprehensive data on the prevalence of quinolone resistance in Mycobacterium tuberculosis clinical isolates in the United States are scarce. By use of a systematic population-based approach, M. tuberculosis strains from tuberculosis (TB) cases were collected in Harris County, TX, in 2007 to 2008. The susceptibilities...

journal_title：Journal of clinical microbiology

authors： El Sahly HM,Teeter LD,Jost KC Jr,Dunbar D,Lew J,Graviss EA

更新日期：2011-08-01 00:00:00

abstract：:A new integrated extraction and real-time PCR-based system for the detection of group B streptococci in antepartum screening samples enriched in Lim broth was compared to the CDC-recommended culture method. The BD Max GBS assay exhibited acceptable sensitivity (95%) and specificity (96.7%) compared to those of the cul...

journal_title：Journal of clinical microbiology

authors： Riedlinger J,Beqaj SH,Milish MA,Young S,Smith R,Dodd M,Hankerd RE,Lebar WD,Newton DW

更新日期：2010-11-01 00:00:00

abstract：:This study evaluated the performance of Directigen FluA combined with a 3-day flu screening culture for the detection of influenza virus. This abbreviated protocol was a useful and effective tool and resulted in a substantial reduction in time, effort, and money spent, while not compromising sensitivity of influenza v...

journal_title：Journal of clinical microbiology

authors： Newton DW,Mellen CF,Baxter BD,Atmar RL,Menegus MA

更新日期：2002-11-01 00:00:00

abstract：:A genotyping study of 285 Hybrid Capture 2 low-risk probe cocktail-positive specimens showed cross-reactivity with several untargeted human papillomavirus genotypes. Cross-reactivity was often clinically beneficial due to the detection of untargeted low-risk genotypes. A total of 8.4% of positive results, usually weak...

journal_title：Journal of clinical microbiology

authors： Poljak M,Kocjan BJ,Kovanda A,Lunar MM,Lepej SZ,Planinic A,Seme K,Vince A

更新日期：2009-08-01 00:00:00

abstract：:Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

journal_title：Journal of clinical microbiology

authors： Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

更新日期：2011-09-01 00:00:00