Abstract：
:Classifications of the Campylobacter fetus subspecies fetus and venerealis were first described in 1959 and were based on the source of isolation (intestinal versus genital) and the ability of the strains to proliferate in the genital tract of cows. Two phenotypic assays (1% glycine tolerance and H2S production) were described to differentiate the subspecies. Multiple molecular assays have been applied to differentiate the C. fetus subspecies, but none of these tests is consistent with the phenotypic identification methods. In this study, we defined the core genome and accessory genes of C. fetus, which are based on the closed genomes of five C. fetus strains. Phylogenetic analysis of the core genomes of 23 C. fetus strains of the two subspecies showed a division into two clusters. The phylogenetic core genome clusters were not consistent with the phenotypic classifications of the C. fetus subspecies. However, they were consistent with the molecular characteristics of the strains, which were determined by multilocus sequence typing, sap typing, and the presence/absence of insertion sequences and a type I restriction modification system. The similarity of the genome characteristics of three of the phenotypically defined C. fetus subsp. fetus strains to C. fetus subsp. venerealis strains, when considering the core genome and accessory genes, requires a critical evaluation of the clinical relevance of C. fetus subspecies identification by phenotypic assays.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
van der Graaf-van Bloois L,Miller WG,Yee E,Rijnsburger M,Wagenaar JA,Duim Bdoi
10.1128/JCM.01837-14subject
Has Abstractpub_date
2014-12-01 00:00:00pages
4183-8issue
12eissn
0095-1137issn
1098-660Xpii
JCM.01837-14journal_volume
52pub_type
杂志文章abstract：:Bacteroides fragilis is an obligatory anaerobic, gram-negative bacterium found among the normal intestinal flora of humans. Enterotoxigenic strains of B. fragilis (ETBF) have been associated with diarrheal diseases in humans and animals. The enterotoxin of ETBF induces fluid changes in ligated intestinal segments and ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.36.12.3545-3548.1998
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abstract：:A rapid colorimetric method for the identification of pathogenic Neisseria (Identicult-Neisseria; Scott Laboratories, Inc.) based on beta-galactosidase, gamma-glutamylaminopeptidase, and gamma-prolylaminopeptidase is described. All 82 clinical isolates of Neisseria gonorrhoeae, 9 clinical isolates of N. meningitidis, ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.24.1.141-142.1986
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.25.11.2218-2220.1987
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.15.3.535-537.1982
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.25.8.1456-1458.1987
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.31.11.3056-3057.1993
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journal_title：Journal of clinical microbiology
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更新日期：1981-03-01 00:00:00
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journal_title：Journal of clinical microbiology
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
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更新日期：1988-04-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.26.3.557-563.1988
更新日期：1988-03-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.34.5.1083-1085.1996
更新日期：1996-05-01 00:00:00
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journal_title：Journal of clinical microbiology
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doi：
更新日期：1975-12-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.27.5.1050-1054.1989
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journal_title：Journal of clinical microbiology
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.18.2.258-269.1983
更新日期：1983-08-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
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journal_title：Journal of clinical microbiology
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doi：10.1128/JCM.33.1.76-78.1995
更新日期：1995-01-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
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