Abstract:
:The sensitivities of BHK-21 (C-13) and murine neuroblastoma (C-1300; clone NA) cells for the isolation of small quantities of a street strain rabies virus were compared. Suspensions of brain from mice sacrificed prior to the onset of clinical signs of rabies were used to stimulate weakly positive diagnostic specimens. The results of cell culture isolation were compared with those of the direct fluorescent-antibody test and virus isolation in weanling mice. Neuroblastoma cells were more sensitive to the street strain rabies virus than were BHK-21 cells. Neuroblastoma cell virus isolation, the mouse inoculation test, and the fluorescent-antibody test all showed comparable sensitivity.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Rudd RJ,Trimarchi CVdoi
10.1128/JCM.25.8.1456-1458.1987subject
Has Abstractpub_date
1987-08-01 00:00:00pages
1456-8issue
8eissn
0095-1137issn
1098-660Xjournal_volume
25pub_type
杂志文章abstract::A dot immunobinding assay that uses inactivated antigen for the detection of rabies viral antibodies was compared with the rapid fluorescent focus inhibition test. Results of testing pre- and postvaccination sera from humans (n = 33) and canines (n = 22) were identical for both tests. Endpoint titers of positive sera ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.7.1262-1264.1987
更新日期:1987-07-01 00:00:00
abstract::A human astrovirus (HAstV) strain from Kenya was characterized by nucleotide sequence analysis. Sequences from open reading frame 1a (ORF1a) clustered with genotype 6/7, those from ORF1b clustered with genotype 3, and those from ORF2 clustered with genotype 2. A recombination point in the ORF1b-ORF2 junction was ident...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01093-10
更新日期:2011-02-01 00:00:00
abstract::Anaerobes are an important but uncommon cause of bloodstream infections (BSIs). For pediatric patients, routine inclusion of an anaerobic blood culture alongside the aerobic remains controversial. We implemented automatic anaerobic blood culture alongside aerobic blood cultures in a pediatric emergency department (ED)...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01844-19
更新日期:2020-08-24 00:00:00
abstract::A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.3.430-434.1990
更新日期:1990-03-01 00:00:00
abstract::The genetic composition and antibiotic sensitivities of 50 clinical isolates of Staphylococcus aureus obtained from various clinics in the Republic of Georgia were characterized. S. aureus strains ATCC 700699 and ATCC 29737 were included as reference standards in all analyses. All 52 strains had identical 16S rRNA pro...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01030-06
更新日期:2006-10-01 00:00:00
abstract::Rickettsiae are gram-negative, obligate intracellular bacteria which have historically been divided into three groups: the typhus group, the scrub typhus group, and the spotted fever group (SFG). Recently, several new SFG rickettsiae have been characterized, and most of these species are associated with ticks and have...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.7.1715-1721.1997
更新日期:1997-07-01 00:00:00
abstract::Viridans streptococci (VS) are common etiologic agents of subacute infective endocarditis and are capable of causing a variety of pyogenic infections. Many species of VS are difficult to differentiate by phenotypic traits. An oligonucleotide array based on 16S-23S rRNA gene intergenic spacer (ITS) sequences was develo...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.4.1515-1521.2005
更新日期:2005-04-01 00:00:00
abstract::The correlation of β-glucan (BG) levels with clinical outcomes in invasive candidiasis (IC) remains unknown. Patients with proven IC were followed prospectively from diagnosis to outcome with twice-weekly serum BG sampling. Correlation of BG with clinical outcome was assessed in each patient. BG levels tend to decreas...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00773-12
更新日期:2012-06-01 00:00:00
abstract::An enzyme-linked immunosorbent assay for the detection of antibodies to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The enzyme-linked immunosorbent assay can also be specific for human immunoglobulin M antibodies when a mu-chain-specific antiglobulin-enzyme conj...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract::The epidemiology of 278 strains of Salmonella typhimurium isolated from 1973 to 1981 from animals in New York State was studied by using four "fingerprinting" techniques, bacteriophage type (B.R. Callow, J. Hyg. 57:346-359, 1959), biotype (J. P. Duguid, E. S. Anderson, G. A. Alfredsson, R. Barker, and D. C. Old, J. Me...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.4.622-627.1989
更新日期:1989-04-01 00:00:00
abstract::Human immunodeficiency virus type 1 serotype C was found in 545 of 712 Ethiopian patients by peptide enzyme immunoassay. Serotyping failed in 146 samples due to the absence of V3 antibodies or multiple reactivities. In 6 of 34 samples, discordant results were obtained by serotyping and genotyping, possibly due to dive...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.9.2419-2421.1997
更新日期:1997-09-01 00:00:00
abstract::Based on 16S rRNA gene PCR, no significant difference was observed in rates of detection of Helicobacter species in intestinal biopsy specimens from 160 Chinese inflammatory bowel disease (IBD) patients (10%) and 80 controls (6.3%). By using a [(13)C]urea breath test, the H. pylori infection rate in 208 Chinese IBD pa...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02630-10
更新日期:2011-05-01 00:00:00
abstract::Thirty-six Shiga toxin-producing Escherichia coli (STEC) O111:H- strains, 18 of which were isolated from patients with hemolytic-uremic syndrome (HUS) and 18 from patients suffering from diarrhea, were investigated for their enterohemolytic phenotypes and genotypes. Twenty-two strains were EHEC hemolysin (EHEC Hly) po...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.10.2364-2367.1996
更新日期:1996-10-01 00:00:00
abstract::Typing and in vitro cultivation of rotavirus-positive human stool samples stored unintentionally at ambient tropical temperatures for 2 1/2 months showed that rotavirus is stable and may still be infectious in vitro. This indicates that stool specimen collection for rotavirus studies can be performed in areas and sett...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.12.4763-4764.2002
更新日期:2002-12-01 00:00:00
abstract::Nonfermenting coryneform bacteria identified as Brevibacterium spp. were isolated from routine clinical specimens. Four strains were derived from peritoneal fluid and has presumably been involved in the pathogenesis of continuous ambulatory peritoneal dialysis peritonitis. Another five isolates most probably represent...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.6.1408-1412.1993
更新日期:1993-06-01 00:00:00
abstract::The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regula...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.5.1587-1590.1999
更新日期:1999-05-01 00:00:00
abstract::Tests for Chlamydia trachomatis and Neisseria gonorrhoeae, which can provide results rapidly to guide therapeutic decision-making, offer patient care advantages over laboratory-based tests that require several days to provide results. We compared results from the Cepheid GeneXpert CT/NG (Xpert) assay to results from t...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.03461-12
更新日期:2013-06-01 00:00:00
abstract::Recent studies have resulted in the proposal of a new species, Aeromonas schubertii (mannitol, sucrose, and indole negative), formerly termed Enteric Group 501, on the basis of the study of seven strains isolated from the southeastern and southwestern United States and Puerto Rico. We have isolated two phenotypically ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.8.1826-1830.1989
更新日期:1989-08-01 00:00:00
abstract::A radioimmunoassay for determination of human immunoglobulin G, A, and M class antibodies to Histoplasma capsulatum is reported. Yeast-phase antigen from H. capsulatum was adsorbed onto polyvinyl chloride microtiter plates as the solid phase. Human antibodies were allowed to react with this antigen, followed by additi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.13.1.66-72.1981
更新日期:1981-01-01 00:00:00
abstract::A rapid, sensitive counterimmunoelectrophoresis assay was developed to detect adenovirus in stools of patients with gastroenteritis. The parameters of the assay were optimized, and its sensitivity and efficiency was examined. The assay promises to be a useful alternative to conventional techniques of cell culture isol...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.10.2.253-255.1979
更新日期:1979-08-01 00:00:00
abstract::We report the first case of spontaneous intrapartum Atopobium vaginae bacteremia identified by 16S rRNA gene sequencing. The bacterium was misidentified by RapID ANA II, API Rapid ID 32A, and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The likely source of bacteremia wa...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00212-12
更新日期:2012-07-01 00:00:00
abstract::For testing bacterial susceptibility to PD131628, a 5-micrograms disk and the following tentative interpretive criteria may be used: > or = 19 mm for susceptible (MIC, < or = 1.0 micrograms/ml), 16 to 18 mm for intermediate (MIC, 2.0 micrograms/ml), and < or = 15 mm for resistant (MIC, > or = 4.0 micrograms/ml). For s...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.1.235-238.1995
更新日期:1995-01-01 00:00:00
abstract::Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors. We analyzed the effect of SPS on the three complement activation pathwa...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01985-09
更新日期:2010-03-01 00:00:00
abstract::We used several molecular typing methods to analyze 196 methicillin-resistant Staphylococcus aureus (MRSA) and 139 methicillin-susceptible S. aureus (MSSA) isolates collected between 1996 and 2005. The sequence type 72 MRSA has increased in frequency in the community in the Republic of Korea and in hospitals in recent...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00098-11
更新日期:2011-05-01 00:00:00
abstract::The human cytomegalovirus (HCMV) a-sequence (a-seq) is located in the joining region between the long (L) and short (S) unique sequences of the virus (L-S junction), and this hypervariable junction has been used to differentiate HCMV strains. The purpose of this study was to investigate whether there are differences a...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.12.2602-2607.1990
更新日期:1990-12-01 00:00:00
abstract::A total of 215 typical strains of Candida albicans were studied for their ability to assimilate 11 carbon compounds. All isolates assimilated lactic acid, ribitol, succinic acid, methyl alpha-D-glucopyranoside. None of the isolates assimilated cellobiose and salicin; 1.9% of the isolates assimilated L-arabinose. Citri...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.13.1.163-166.1981
更新日期:1981-01-01 00:00:00
abstract::Three bovine group B rotavirus strains were detected from diarrheic calves during a surveillance study of rotaviral diarrhea in West Bengal, India. The sequence analysis of VP7 and NSP5 genes of these strains demonstrates a high degree of sequence variation from other group B rotavirus strains, indicating the emergenc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.6.2816-2818.2004
更新日期:2004-06-01 00:00:00
abstract::The use of high-risk human papillomavirus (HPV) testing for surveillance and clinical applications is increasing globally, and it is important that tests are evaluated to ensure they are fit for this purpose. In this study, the performance of a new HPV genotyping test, the Papilloplex high-risk HPV (HR-HPV) test, was ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01687-17
更新日期:2018-02-22 00:00:00
abstract::In a follow-up study, enterotoxigenic Escherichia coli (ETEC) infections in 145 children from two communities located in northeastern Argentina were monitored for 2 years. The occurrence of diarrhea was monitored by weekly household visits. Of 730 fecal specimens collected, 137 (19%) corresponded to diarrheal episodes...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.9.2829-2833.1999
更新日期:1999-09-01 00:00:00
abstract::Relative amounts of D-arabinitol (fungal origin) and L-arabinitol (part of normal human metabolism) in urine were determined by gas chromatography and mass spectrometry from 61 hospitalized patients with hematological malignancies. Seventeen neutropenic patients with acute leukemia (with 53 samples) had disseminated y...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.9.2175-2179.1996
更新日期:1996-09-01 00:00:00