Serodiagnosis of rabies by dot immunobinding assay.

abstract：:Clostridium difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis, which have significant morbidity and mortality. Accurate and timely diagnosis is critical. Repeat enzyme immunoassay testing for C. difficile toxin has been recommended because of <100% sensitivity. All C. diffic...

journal_title：Journal of clinical microbiology

authors： Cardona DM,Rand KH

更新日期：2008-11-01 00:00:00

abstract：:Monoclonal antibodies (Mabs) specific for Haemophilus influenzae were generated to identify antigenic determinants shared among encapsulated H. influenzae clones. Sixteen MAbs reacted by Western immunoblot with a protein of an approximate molecular size of 40 kilodaltons corresponding to the P2 major outer membrane pr...

journal_title：Journal of clinical microbiology

authors： Martin D,Hamel J,Brodeur BR,Musser JM

更新日期：1990-08-01 00:00:00

abstract：:The beta and gamma genera of papillomaviruses consist of epidermodysplasia verruciformis-related human papillomaviruses (HPVs) and phylogenetically related cutaneous HPVs. Here, we have developed a consensus primer PCR assay and reverse line blot typing system coupled thereto (referred to as beta and gamma cutaneous H...

journal_title：Journal of clinical microbiology

authors： Brink AA,Lloveras B,Nindl I,Heideman DA,Kramer D,Pol R,Fuente MJ,Meijer CJ,Snijders PJ

更新日期：2005-11-01 00:00:00

abstract：:In a field trial conducted in cooperation with pediatricians in private practice, 198 nasopharyngeal swabs from children with suspected whooping cough were placed into charcoal-horse-blood transport medium with cephalexin (40 mg/liter). After preincubation at 36 degrees C for 1 to 2 days, the transport systems were ma...

journal_title：Journal of clinical microbiology

authors： Hoppe JE,Weiss A,Wörz S

更新日期：1988-06-01 00:00:00

abstract：:Streptococcus agalactiae causes severe invasive disease in humans and mastitis in cattle. Temporally matched bovine milk isolates and clinical human invasive isolates (52 each) collected in New York State over 18 months were characterized by molecular subtyping and phenotypic methods to probe the interspecies transmis...

journal_title：Journal of clinical microbiology

authors： Sukhnanand S,Dogan B,Ayodele MO,Zadoks RN,Craver MP,Dumas NB,Schukken YH,Boor KJ,Wiedmann M

更新日期：2005-03-01 00:00:00

abstract：:Pneumonia has been identified as a major cause of poor lamb survival in indigenous herds of Rocky Mountain bighorn sheep (Ovis canadensis canadensis) in central Idaho. Pasteurella haemolytica was isolated from five adult Rocky Mountain bighorn ewes captured from a free-ranging herd in central Idaho. The lambs from two...

journal_title：Journal of clinical microbiology

authors： Jaworski MD,Ward AC,Hunter DL,Wesley IV

更新日期：1993-04-01 00:00:00

abstract：:Whole-genome sequencing (WGS) is a highly sensitive method for identifying genetic relatedness and transmission of Clostridioides difficile strains. Previous studies suggest that as few as 3 core genome single-nucleotide variants (SNVs) discriminate between genetically distinct isolates. Because a single C. difficile ...

journal_title：Journal of clinical microbiology

authors： Balaji A,Ozer EA,Kociolek LK

更新日期：2019-08-26 00:00:00

abstract：:Mycobacterium tilburgii is a nonculturable nontuberculous mycobacterium identifiable only by molecular methods. We report a case of disseminated M. tilburgii infection illustrating the importance of 16S rRNA gene sequencing to determine the responsible mycobacterial pathogen and the difficulties in tailoring antimycob...

journal_title：Journal of clinical microbiology

authors： Temmerman S,Vandekerckhove L,Sermijn E,Vogelaers D,Claeys G,Vaneechoutte M,Cools P,Callens S

更新日期：2014-05-01 00:00:00

abstract：:HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific f...

journal_title：Journal of clinical microbiology

authors： Estrade C,Sahli R

更新日期：2014-11-01 00:00:00

abstract：:We evaluated the Phoenix automated microbiology system (BD Diagnostic Systems, Sparks, MD) for the identification (ID) and antimicrobial susceptibility testing (AST) of challenge and clinical staphylococci and enterococci recovered from patients in a tertiary-care medical center. In total, 424 isolates were tested: 90...

journal_title：Journal of clinical microbiology

authors： Carroll KC,Borek AP,Burger C,Glanz B,Bhally H,Henciak S,Flayhart DC

更新日期：2006-06-01 00:00:00

abstract：:A Salmonella enterica serotype Typhi strain was cultured from blood and fecal samples from a 54-year-old man with fever and diarrhea. He had returned from travel to the Philippines a few days earlier. Phenotypic and genotypic analysis confirmed the production of the SHV-12 extended-spectrum beta-lactamase. ...

journal_title：Journal of clinical microbiology

authors： Al Naiemi N,Zwart B,Rijnsburger MC,Roosendaal R,Debets-Ossenkopp YJ,Mulder JA,Fijen CA,Maten W,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2008-08-01 00:00:00

abstract：:Candida auris is a multidrug-resistant yeast that causes a wide spectrum of infections, especially in intensive care settings. We investigated C. auris prevalence among 102 clinical isolates previously identified as Candida haemulonii or Candida famata by the Vitek 2 system. Internal transcribed spacer region (ITS) se...

journal_title：Journal of clinical microbiology

authors： Kathuria S,Singh PK,Sharma C,Prakash A,Masih A,Kumar A,Meis JF,Chowdhary A

更新日期：2015-06-01 00:00:00

abstract：:An immunoblot assay for the serological diagnosis of Helicobacter pylori infection was evaluated. Serum samples from patients whose gastric biopsy specimens were known to be positive or negative for H. pylori on culture were used to establish interpretive criteria for the immunoblot assay. A panel of sera from patient...

journal_title：Journal of clinical microbiology

authors： Nilsson I,Ljungh A,Aleljung P,Wadström T

更新日期：1997-02-01 00:00:00

abstract：:Triplicate preserved fecal samples from 265 patients were pooled into single samples, and the recovery rate of intestinal parasites from the pooled samples was compared with that from the individual samples. Parasites were detected in 109 patients when results from the individual samples were used compared with 108 pa...

journal_title：Journal of clinical microbiology

authors： Aldeen WE,Shisenant J,Hale D,Matsen J,Carroll K

更新日期：1993-01-01 00:00:00

abstract：:Clostridium difficile is a Gram-positive bacterium commonly found in health care and long-term-care facilities and is the most common cause of antibiotic-associated diarrhea. Rapid detection of this bacterium can assist physicians in implementing contact precautions and appropriate antibiotic therapy in a timely manne...

journal_title：Journal of clinical microbiology

authors： Beck ET,Buchan BW,Riebe KM,Alkins BR,Pancholi P,Granato PA,Ledeboer NA

更新日期：2014-06-01 00:00:00

abstract：:A qualitative multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was developed. Furthermore, a separate assay for the recognition of hepatitis A virus was established to complement the test pattern so that all ...

journal_title：Journal of clinical microbiology

authors： Jokela P,Joki-Korpela P,Maaronen M,Glumoff V,Hyypiä T

更新日期：2005-03-01 00:00:00

abstract：:Resistance to extended-spectrum β-lactam antibiotics has led to a greater reliance upon carbapenems, but the expression of carbapenemases threatens to limit the utility of these drugs. Current methods to detect carbapenemase activity are suboptimal, requiring prolonged incubations during which ineffective therapy may ...

journal_title：Journal of clinical microbiology

authors： Kulkarni MV,Zurita AN,Pyka JS,Murray TS,Hodsdon ME,Peaper DR

更新日期：2014-07-01 00:00:00

abstract：:Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes co...

journal_title：Journal of clinical microbiology

authors： Kim YG,Yun SG,Kim MY,Park K,Cho CH,Yoon SY,Nam MH,Lee CK,Cho YJ,Lim CS

更新日期：2016-12-28 00:00:00

abstract：:Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necro...

journal_title：Journal of clinical microbiology

authors： Van Bost S,Jacquemin E,Oswald E,Mainil J

更新日期：2003-09-01 00:00:00

abstract：:The API 20S system (Analytab Products, Plainview, N.Y.) and the AutoMicrobic Gram-Positive Identification system (GPI; Vitek Systems, Hazelwood, Mo.) were evaluated for their capacity to identify the non-beta-hemolytic streptococci and aerococci to the species level. The 20S system identified 86% (six of seven strains...

journal_title：Journal of clinical microbiology

authors： Facklam R,Bosley GS,Rhoden D,Franklin AR,Weaver N,Schulman R

更新日期：1985-04-01 00:00:00

abstract：:Shiga toxin (Stx)-producing Escherichia coli (STEC) is the main cause of postdiarrheal hemolytic-uremic syndrome (HUS), a life-threatening clinical complication characterized by hemolytic anemia, thrombocytopenia, and acute renal failure that mainly affects children. A relevant feature of STEC strains is the productio...

journal_title：Journal of clinical microbiology

authors： Melli LJ,Zylberman V,Hiriart Y,Lauche CE,Baschkier A,Pardo R,Miliwebsky E,Chinen I,Rivas M,Goldbaum FA,Ugalde JE,Comerci DJ,Ciocchini AE

更新日期：2020-02-24 00:00:00

abstract：:We found 31 different emm-toxin genotypes among 74 group A streptococcal isolates causing invasive infections in French children. The predominant emm types were emm1 (25%), emm3 (8%), emm4 (8%), emm6 (7%), and emm89 (9%). Sixteen percent of isolates harbored the streptococcal invasive locus, half of them belonging to ...

journal_title：Journal of clinical microbiology

authors： Bidet P,Courroux C,Salgueiro C,Carol A,Mariani-Kurkdjian P,Bonacorsi S,Bingen E

更新日期：2007-06-01 00:00:00

abstract：:Total DNA from five Campylobacter species was digested with a mixture of XhoI and BglII restriction endonucleases and analyzed by Southern hybridization by using a probe complementary to the DNA coding for the 16S rRNA. Each of the Campylobacter species, including C. jejuni, C. coli, C. laridis, C. fetus, and C. upsal...

journal_title：Journal of clinical microbiology

authors： Moureau P,Derclaye I,Gregoire D,Janssen M,Cornelis GR

更新日期：1989-07-01 00:00:00

abstract：:The aim of this study was to determine the distribution in France of the Enterobacter aerogenes prevalent clone isolated in the hospitals of the Marseille area (A. Davin-Regli, D. Monnet, P. Saux, C. Bosi, R. Charrel, A. Barthelemy, and C. Bollet, J. Clin. Microbiol. 34:1474-1480, 1996). A total of 123 E. aerogenes is...

journal_title：Journal of clinical microbiology

authors： Bosi C,Davin-Regli A,Bornet C,Mallea M,Pages JM,Bollet C

更新日期：1999-07-01 00:00:00

abstract：:A mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis and its nonmucoid revertant were grown in a chemically defined alginate-promoting medium under batch and continuous culture conditions. Selection for the mucoid and nonmucoid phenotype was accomplished by varying the levels of air a...

journal_title：Journal of clinical microbiology

authors： Krieg DP,Bass JA,Mattingly SJ

更新日期：1986-12-01 00:00:00

abstract：:We have developed a PCR assay for one-step differentiation of the three complexes of New World Leishmania (Leishmania braziliensis, Leishmania mexicana, and Leishmania donovani). This multiplex assay is targeted to the spliced leader RNA (mini-exon) gene repeats of these organisms and can detect all three complexes si...

journal_title：Journal of clinical microbiology

authors： Harris E,Kropp G,Belli A,Rodriguez B,Agabian N

更新日期：1998-07-01 00:00:00

abstract：:We developed a loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y. pseudotuberculosis. ...

journal_title：Journal of clinical microbiology

authors： Horisaka T,Fujita K,Iwata T,Nakadai A,Okatani AT,Horikita T,Taniguchi T,Honda E,Yokomizo Y,Hayashidani H

更新日期：2004-11-01 00:00:00

abstract：:A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was...

journal_title：Journal of clinical microbiology

authors： Rench MA,Metzger TG,Baker CJ

更新日期：1984-11-01 00:00:00

abstract：:The algorithm for a new identification system was designed on the basis of colony color and morphology on CHROMagar Orientation medium in conjunction with simple biochemical tests such as indole (IND), lysine decarboxylase (LDC), and ornithine decarboxylase (ODC) utilization tests with gram-negative bacilli isolated f...

journal_title：Journal of clinical microbiology

authors： Ohkusu K

更新日期：2000-12-01 00:00:00

abstract：:Monoclonal antibodies to K88ac and K88ab were used in enzyme-linked immunosorbent assays on Escherichia coli cultures known to produce K88 pili. A total of 415 K88-positive E. coli isolates from nine states were all found to be the K88ac variant. The cultures tested were isolated during the years 1976 to 1985. ...

journal_title：Journal of clinical microbiology

authors： Westerman RB,Mills KW,Phillips RM,Fortner GW,Greenwood JM

更新日期：1988-01-01 00:00:00