Comparison of pooled formalin-preserved fecal specimens with three individual samples for detection of intestinal parasites.

abstract：:The use of molecular identification techniques has revealed an increasing number of new species within Aspergillus section Terrei. We phenotyped a set of 26 clinical isolates that showed genetic differences from Aspergillus terreus sensu stricto by analyzing sequences from PCR-amplified β-tubulin and calmodulin genes ...

journal_title：Journal of clinical microbiology

authors： Guinea J,Sandoval-Denis M,Escribano P,Peláez T,Guarro J,Bouza E

更新日期：2015-02-01 00:00:00

abstract：:Despite attempts to control avian mycoplasmosis through management, vaccination, and surveillance, Mycoplasma gallisepticum continues to cause significant morbidity, mortality, and economic losses in poultry production. Live attenuated vaccines are commonly used in the poultry industry to control avian mycoplasmosis; ...

journal_title：Journal of clinical microbiology

authors： Ricketts C,Pickler L,Maurer J,Ayyampalayam S,García M,Ferguson-Noel NM

更新日期：2016-12-28 00:00:00

abstract：:Formalin-fixed, purified reticulate bodies (RB) of Chlamydia trachomatis immunotype C/TW-3/OT were used as a serological test antigen in the microimmunofluorescence test. The sensitivity and specificity of the RB antigen were compared to elementary bodies (EB) used as antigens in the detection of C. trachomatis antibo...

journal_title：Journal of clinical microbiology

authors： Yong EC,Chinn JS,Caldwell HD,Kuo CC

更新日期：1979-09-01 00:00:00

abstract：:Cytomegalovirus (CMV) DNA loads in paired leukocyte and plasma samples from 199 patient visits by 66 patients with CMV retinitis were determined. Leukocyte CMV load determinations had a greater range of values (mean, 24,587 copies/10(6) leukocytes; maximum, 539, 000) than did plasma CMV load determinations (mean, 10,3...

journal_title：Journal of clinical microbiology

authors： Jabs DA,Forman M,Enger C,Jackson JB

更新日期：1999-05-01 00:00:00

abstract：:Stable dwarf forms of Staphylococcus aureus have been identified in clinical specimens as the sole or predominant isolate in eight cases. These organisms have been shown to be menadione or thiamine dependent, i.e., cultivation in the presence of one of these agents has permitted growth of colonies which appear typical...

journal_title：Journal of clinical microbiology

authors： Acar JF,Goldstein FW,Lagrange P

更新日期：1978-08-01 00:00:00

abstract：:A bacitracin (0.04 units, BBL)-SXT (trimethoprim, 1.25 mg, plus sulfamethoxazole, 23.75 mg, BBL) susceptibility test was 94% accurate in presumptively identifying streptococci as either group A, B or not group A and B. ...

journal_title：Journal of clinical microbiology

authors： Gunn BA

更新日期：1976-08-01 00:00:00

abstract：:Bartonella henselae uveitis was diagnosed in a 40-year-old woman with underlying HLA-B27 uveitis on the basis of immunodetection and molecular detection of the organism, a Marseilles genogroup and a CAL-1 genotype strain, in the vitreous fluid. This case illustrates that B. henselae should be included in the different...

journal_title：Journal of clinical microbiology

authors： Drancourt M,Bodaghi B,Lepidi H,Le Hoang P,Raoult D

更新日期：2004-04-01 00:00:00

abstract：:To provide more sensitive and convenient methods for the detection of equine infectious anemia virus (EIAV), we developed an enzyme-linked immunosorbent assay (ELISA) employing the EIAV gag precursor (Pr55gag) produced by using recombinant DNA techniques. The antigenic reactivity of the recombinant EIAV Pr55gag was fo...

journal_title：Journal of clinical microbiology

authors： Archambault D,Wang ZM,Lacal JC,Gazit A,Yaniv A,Dahlberg JE,Tronick SR

更新日期：1989-06-01 00:00:00

abstract：:Although numerous perioperative samples and culture media are required to diagnose prosthetic joint infection (PJI), their exact number and types have not yet been definitely determined with a high level of proof. We conducted a prospective multicenter study to determine the minimal number of samples and culture media...

journal_title：Journal of clinical microbiology

authors： Bémer P,Léger J,Tandé D,Plouzeau C,Valentin AS,Jolivet-Gougeon A,Lemarié C,Kempf M,Héry-Arnaud G,Bret L,Juvin ME,Giraudeau B,Corvec S,Burucoa C,Centre de Référence des Infections Ostéo-articulaires du Grand Ouest (CRIOGO) Stu

更新日期：2016-02-01 00:00:00

abstract：:The microbiological and molecular characteristics of the rickettsiae isolated from humans with Queensland tick typhus (QTT) caused by Rickettsia australis and the recently described Flinders Island spotted fever (FISF) were compared. Clinically and serologically, the diseases are similar. Cell culture reveals differen...

journal_title：Journal of clinical microbiology

authors： Baird RW,Lloyd M,Stenos J,Ross BC,Stewart RS,Dwyer B

更新日期：1992-11-01 00:00:00

abstract：:Detection of the source of Clostridium difficile strains is of importance for the control of the nosocomial spread of this microorganism. For this purpose, vaginal and rectal swabs from 183 mothers, duplicate fecal samples (taken on days 1 and 4 after birth) from 183 neonates, and 94 environmental samples were culture...

journal_title：Journal of clinical microbiology

authors： Martirosian G,Kuipers S,Verbrugh H,van Belkum A,Meisel-Mikolajczyk F

更新日期：1995-08-01 00:00:00

abstract：:Reverse transcription-PCR (RT-PCR) is a sensitive method for detection of RNA virus nucleic acid sequences in clinical respiratory specimens. Previous studies have focused on RT-PCR for a single virus, but this approach is limited by the inability to establish a specific etiology when the RT-PCR result is negative and...

journal_title：Journal of clinical microbiology

authors： Gilbert LL,Dakhama A,Bone BM,Thomas EE,Hegele RG

更新日期：1996-01-01 00:00:00

abstract：:Two DNA probes, one 1.1- and one 0.75-kb probe, specific for Serpulina hyodysenteriae were isolated from a genomic library generated from virulent S. hyodysenteriae 5380. These probes are highly specific and react with all S. hyodysenteriae strains tested. Under stringent conditions, the DNA probes did not react with ...

journal_title：Journal of clinical microbiology

authors： Sotiropoulos C,Smith SC,Coloe PJ

更新日期：1993-07-01 00:00:00

abstract：:Cross-reactivity between Legionella spp. and Capnocytophaga ochracea was noted by latex agglutination tests (Serobact Legionella; Disposable Products, Adelaide, Australia). Four of 11 (36%) C. ochracea isolates agglutinated with latex reagents designed to identify Legionella pneumophila serogroups. C. ochracea isolate...

journal_title：Journal of clinical microbiology

authors： Chen S,Hicks L,Yuen M,Mitchell D,Gilbert GL

更新日期：1994-12-01 00:00:00

abstract：:Toxoplasmic lymphadenopathy (TL) is the most common clinical manifestation of acute acquired toxoplasma infection in normal individuals. The diagnosis is established by serologic methods and lymph node biopsy. Recently, tests for avidity of toxoplasma immunoglobulin G (IgG) antibodies have been introduced to help disc...

journal_title：Journal of clinical microbiology

authors： Montoya JG,Huffman HB,Remington JS

更新日期：2004-10-01 00:00:00

abstract：:We report the first documented case of endocarditis in a man infected with Bartonella alsatica, which causes bacteremia in healthy wild rabbits. B. alsatica was identified by serology and culture and by PCR of an aortic valve specimen. B. alsatica should be added to the list of zoonotic agents of blood culture-negativ...

journal_title：Journal of clinical microbiology

authors： Raoult D,Roblot F,Rolain JM,Besnier JM,Loulergue J,Bastides F,Choutet P

更新日期：2006-01-01 00:00:00

abstract：:The Elecsys Anti-SARS-CoV-2 immunoassay (Roche Diagnostics) was developed to provide accurate, reliable detection of antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We evaluated sensitivity, specificity, cross-reactivity, and agreement with a vesicular stomatitis virus-based pseudoneutraliz...

journal_title：Journal of clinical microbiology

authors： Muench P,Jochum S,Wenderoth V,Ofenloch-Haehnle B,Hombach M,Strobl M,Sadlowski H,Sachse C,Torriani G,Eckerle I,Riedel A

更新日期：2020-09-22 00:00:00

abstract：:The rate of false-positive hepatitis C virus enzyme immunoassay results was determined to be at least 10% among 1,814 reactive serum samples based on (i) negative results in an independent confirmation assay, (ii) negative PCR results, and (iii) no patients developing clinical or biochemical signs of hepatitis during ...

journal_title：Journal of clinical microbiology

authors： Schröter M,Feucht HH,Schäfer P,Zöllner B,Polywka S,Laufs R

更新日期：1999-01-01 00:00:00

abstract：:Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a ...

journal_title：Journal of clinical microbiology

authors： Röder B,Frühwirth K,Vogl C,Wagner M,Rossmanith P

更新日期：2010-11-01 00:00:00

abstract：:Morganella ("Proteus") morganii is the only species in the recently proposed genus Morganella, but we suspect there are yet undescribed species in the genus. Two candidates for new species were recently investigated. Nineteen strains isolated from clinical specimens resembled M. morganii but were lysine positive and n...

journal_title：Journal of clinical microbiology

authors： Hickman FW,Framer JJ 3rd,Steigerwalt AG,Brenner DJ

更新日期：1980-07-01 00:00:00

abstract：:A rapid colorimetric method for the identification of pathogenic Neisseria (Identicult-Neisseria; Scott Laboratories, Inc.) based on beta-galactosidase, gamma-glutamylaminopeptidase, and gamma-prolylaminopeptidase is described. All 82 clinical isolates of Neisseria gonorrhoeae, 9 clinical isolates of N. meningitidis, ...

journal_title：Journal of clinical microbiology

authors： Hosmer ME,Cohenford MA,Ellner PD

更新日期：1986-07-01 00:00:00

abstract：:A combined method of the Limulus amebocyte lysate (LAL) test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigenicity of coagulogen, a major protein in LAL, was lost when LAL reacted with endotoxin as shown by immunoblotting. Determination of ...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Koch C

更新日期：1988-08-01 00:00:00

abstract：:A transferable plasmid encoding SHV-12 extended-spectrum beta-lactamase, TEM-116, and aminoglycoside resistance was responsible for two sequential clonal outbreaks of Enterobacter cloacae and Acinetobacter baumannii bacteria. A similar plasmid was present among isolates of four different bacterial species. Recognition...

journal_title：Journal of clinical microbiology

authors： Naiemi NA,Duim B,Savelkoul PH,Spanjaard L,de Jonge E,Bart A,Vandenbroucke-Grauls CM,de Jong MD

更新日期：2005-09-01 00:00:00

abstract：:Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less t...

journal_title：Journal of clinical microbiology

authors： Hendry C,Dionne K,Hedgepeth A,Carroll K,Parrish N

更新日期：2009-04-01 00:00:00

abstract：:A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successfully detected and c...

journal_title：Journal of clinical microbiology

authors： Wu Y,Tan EL,Yeo A,Chan KP,Nishimura H,Cardosa MJ,Poh CL,Quak SH,Chow VT

更新日期：2011-01-01 00:00:00

abstract：:A mathematical analysis of serological data has provided the basis for a new serotyping routine. This paper describes the process whereby the results of agglutination reactions between the standard O antigens of Escherichia coli and rabbit antisera raised against them were used to produce a much simpler, faster, and c...

journal_title：Journal of clinical microbiology

authors： Thompson CJ

更新日期：1987-05-01 00:00:00

abstract：:An association of enterovirus infection with endemic cardiomyopathy (Keshan disease [KD]) and outbreaks of myocarditis in selenium-deficient rural areas of southwestern China has been established. Enteroviruses have been isolated from patients with KD or during outbreaks of myocarditis in last two decades. Six of thes...

journal_title：Journal of clinical microbiology

authors： Peng T,Li Y,Yang Y,Niu C,Morgan-Capner P,Archard LC,Zhang H

更新日期：2000-10-01 00:00:00

abstract：:Borrelia burgdorferi sensu lato species display considerable antigenic polymorphism. In order to evaluate the importance of this antigenic heterogeneity in the serodiagnosis of Lyme disease, the serum immunoglobulin G response in 148 healthy individuals from an area in northern Sweden where Lyme disease is endemic and...

journal_title：Journal of clinical microbiology

authors： Bunikis J,Olsén B,Westman G,Bergstroöm S

更新日期：1995-06-01 00:00:00

abstract：:The factors that regulate cytomegalovirus (CMV) excretion from the genitourinary tract are poorly understood. To assess the role of cell-mediated immunity in such excretion, a CMV-specific mononuclear blastogenesis assay was used to study a predominantly lower-socioeconomic-status population of 92 healthy nonpregnant ...

journal_title：Journal of clinical microbiology

authors： Faix RG,Zweig SE,Kummer JF,Moore D,Lang DJ

更新日期：1983-04-01 00:00:00

abstract：:An echovirus 3 (Echo3) strain (strain LR31G7) was isolated from a sewage treatment plant in Greece in 2005. Full-genome molecular, phylogenetic, and SimPlot analyses were conducted in order to reveal the evolutionary pathways of the isolate. Nucleotide and phylogenetic analyses of part of the VP1 genomic region reveal...

journal_title：Journal of clinical microbiology

authors： Kyriakopoulou Z,Dedepsidis E,Pliaka V,Tsakogiannis D,Pratti A,Levidiotou-Stefanou S,Markoulatos P

更新日期：2010-05-01 00:00:00