New method of serotyping Escherichia coli: mathematical development.

abstract：:Respiratory tract infections are a leading cause of morbidity and mortality worldwide. Even with the advancement of diagnostic tools, the causative agent of 20 to 30% of upper respiratory tract infections go undiagnosed. Recently, a newly identified human respiratory virus, human metapneumovirus (hMPV), was discovered...

journal_title：Journal of clinical microbiology

authors： Regev L,Hindiyeh M,Shulman LM,Barak A,Levy V,Azar R,Shalev Y,Grossman Z,Mendelson E

更新日期：2006-04-01 00:00:00

abstract：:Tropheryma whipplei is the causative agent of classic Whipple's disease (WD) and other clinical entities, such as localized infection. Asymptomatic carriers have also been reported, mainly based on the testing of fecal samples. Our objective was to undertake a retrospective analysis of molecular biology usage for the ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Fenollar F,Raoult D

更新日期：2012-12-01 00:00:00

abstract：:Rhesus and cynomolgus monkey kidney tissue cultures and two continuous lines, Madin-Darby canine kidney (MDCK) and LLC-MK2, were compared in titrations and isolations of influenza and parainfluenza viruses. Tube cultures were inoculated with laboratory virus strains or stored patient specimens and observed for hemadso...

journal_title：Journal of clinical microbiology

authors： Frank AL,Couch RB,Griffis CA,Baxter BD

更新日期：1979-07-01 00:00:00

abstract：:Three commercial chromogenic agar media were evaluated for Streptococcus agalactiae screening in 200 vaginal swabs from pregnant women. The sensitivity and specificity were 94.3% and 100% for Granada medium (bioMérieux), 100% and 90.3% for Brilliance GBS medium (Thermo Fisher Scientific), and 100% and 98.8% for ChromI...

journal_title：Journal of clinical microbiology

authors： Verhoeven PO,Noyel P,Bonneau J,Carricajo A,Fonsale N,Ros A,Pozzetto B,Grattard F

更新日期：2014-03-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been suggested as a reliable method for bacterial identification from cultures. Direct analysis of clinical samples might increase the usefulness of this method, shortening the time for microorganism identification. We comp...

journal_title：Journal of clinical microbiology

authors： Ferreira L,Sánchez-Juanes F,González-Avila M,Cembrero-Fuciños D,Herrero-Hernández A,González-Buitrago JM,Muñoz-Bellido JL

更新日期：2010-06-01 00:00:00

abstract：:Two rapid diagnostic tests for Chlamydia trachomatis (Microtrak, Syva Co., Palo Alto, Calif.; and Chlamydiazyme, Abbott Laboratories, North Chicago, Ill.) were evaluated in comparison with growth of the organism in tissue culture for 2,030 urogenital specimens from men and women. Neither test performed as well as cult...

journal_title：Journal of clinical microbiology

authors： Hipp SS,Han Y,Murphy D

更新日期：1987-10-01 00:00:00

abstract：:The modification of an auxanographic method used in yeast species identification to determine potassium nitrate (KNO3) assimilation resulted in a simple and inexpensive KNO3 assimilation test medium. The medium provided accurate and reliable results in less than 24 h. ...

journal_title：Journal of clinical microbiology

authors： Pincus DH,Salkin IF,Hurd NJ,Levy IL,Kemna MA

更新日期：1988-02-01 00:00:00

abstract：:Neisseria gonorrhoeae is a common bacterial sexually transmitted infection (STI). Currently, there are limited data on the bacterial load in both men and women and on both genital and extragenital sites. Therefore, we quantified N. gonorrhoeae bacterial loads in a large population of women, heterosexual men, and men w...

journal_title：Journal of clinical microbiology

authors： van der Veer BMJW,Hoebe CJPA,Dukers-Muijrers NHTM,van Alphen LB,Wolffs PFG

更新日期：2020-10-21 00:00:00

abstract：:We prospectively investigated the diagnostic utility of the Xpert MTB/RIF (Mycobacterium tuberculosis/rifampin [RIF] resistance) assay in 20 cases with confirmed tuberculous pleural effusion. The sensitivity and specificity of the Xpert assay in pleural fluid were 25% and 100%, respectively. All cases positive by the ...

journal_title：Journal of clinical microbiology

authors： Friedrich SO,von Groote-Bidlingmaier F,Diacon AH

更新日期：2011-12-01 00:00:00

abstract：:Nineteen reference and 156 clinical strains of the genus Nocardia belonging to 12 taxonomic groups were studied for restriction fragment length polymorphism (RFLP) by using an amplified 439-bp segment of the 65-kDa heat shock protein gene. Of 30 restriction endonucleases, digestion with MspI and then digestion with Bs...

journal_title：Journal of clinical microbiology

authors： Steingrube VA,Brown BA,Gibson JL,Wilson RW,Brown J,Blacklock Z,Jost K,Locke S,Ulrich RF,Wallace RJ Jr

更新日期：1995-12-01 00:00:00

abstract：:A total of 100 random clinical urine specimens were divided and used to compare Becton, Dickinson & Co. B-D Urine Culture Kit-treated urine (BDU) with refrigerated urine (RU). Each RU-BDU pair was cultured with the standard calibrated loop-plate method and also screened with the Abbott Laboratories MS-2 system at 0, 5...

journal_title：Journal of clinical microbiology

authors： Hubbard WA,Shalis PJ,McClatchey KD

更新日期：1983-02-01 00:00:00

abstract：:The Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. Part of this service includes the serogrouping of meningococcal isolates followed by typing and subtyping. The procedures for this ...

journal_title：Journal of clinical microbiology

authors： Clarke SC,Diggle MA,Edwards GF

更新日期：2001-09-01 00:00:00

abstract：:The etiology of Crohn's disease in humans is largely unknown. Clinical signs of Crohn's disease partly resemble the clinical picture of Johne's disease in ruminants caused by Mycobacterium avium subsp. paratuberculosis. Because of the high prevalence of these bacteria in (products of) ruminants and their remarkable th...

journal_title：Journal of clinical microbiology

authors： Overduin P,Schouls L,Roholl P,van der Zanden A,Mahmmod N,Herrewegh A,van Soolingen D

更新日期：2004-11-01 00:00:00

abstract：:The genes encoding the 56-kDa polypeptides were amplified by polymerase chain reaction from the genomic DNAs of three serotypes of Rickettsia tsutsugamushi, Gilliam, Karp, and Boryong. The amplified products were cloned into expression vector pIH821, and the recombinant antigens were expressed in Escherichia coli as f...

journal_title：Journal of clinical microbiology

authors： Kim IS,Seong SY,Woo SG,Choi MS,Kang JS,Chang WH

更新日期：1993-08-01 00:00:00

abstract：:A replicator method for screening Staphylococcus aureus isolates for synergistic hemolytic activity is described. The technique should also be useful for assessing the hemolytic activity of staphylococci and streptococci on different lots or brands of blood agar because the inoculum and the distances between spot inoc...

journal_title：Journal of clinical microbiology

authors： Boyce JM

更新日期：1985-05-01 00:00:00

abstract：:A combination of two systems for the serotyping of Edwardsiella tarda developed independently at the National Institute of Health, Tokyo, Japan, and the Centers for Disease Control, Atlanta, Ga., has enabled a single serotyping scheme comprising 61 O groups and 45 H antigens to be established for international use. ...

journal_title：Journal of clinical microbiology

authors： Tamura K,Sakazaki R,McWhorter AC,Kosako Y

更新日期：1988-11-01 00:00:00

abstract：:This study assessed the diagnostic performance of the new COVID19SEROSpeed IgM/IgG rapid test (BioSpeedia, a spinoff of the Pasteur Institute of Paris) for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in comparison to other commercial antibody assays through a large ...

journal_title：Journal of clinical microbiology

authors： Plebani M,Parčina M,Bechri I,Zehender G,Terkeš V,Abdel Hafith B,Antinori S,Pillet S,Gonzalo S,Hoerauf A,Lai A,Morović M,Bourlet T,Torre A,Pozzetto B,Galli M

更新日期：2021-01-21 00:00:00

abstract：:We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia s...

journal_title：Journal of clinical microbiology

authors： Qurollo BA,Riggins D,Comyn A,Zewde MT,Breitschwerdt EB

更新日期：2014-11-01 00:00:00

abstract：:An examination of 45 clinical isolates of Cryptococcus neoformans revealed an unusually high incidence of C. neoformans var. gattii in South Australia (65%) and in the Northern Territory (95%). In assessing all the available data from Australian isolates of C. neoformans, there appeared to be an endemic focus for the ...

journal_title：Journal of clinical microbiology

authors： Ellis DH

更新日期：1987-02-01 00:00:00

abstract：:Two cyp51-related genes (cyp51A and cyp51B) encoding 14-alpha sterol demethylase-like enzymes were identified in the opportunistic human pathogen Aspergillus fumigatus. PCR amplification using degenerate oligonucleotides based on conserved areas of cytochrome P450 demethylases of other filamentous fungi and yeasts all...

journal_title：Journal of clinical microbiology

authors： Mellado E,Diaz-Guerra TM,Cuenca-Estrella M,Rodriguez-Tudela JL

更新日期：2001-07-01 00:00:00

abstract：:Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferisensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in p...

journal_title：Journal of clinical microbiology

authors： Hahm JB,Breneman JW 4th,Liu J,Rabkina S,Zheng W,Zhou S,Walker RP,Kaul R

更新日期：2020-04-23 00:00:00

abstract：:An international collaborative study was performed in order to propose quality control limits for voriconazole disk diffusion tests on Mueller-Hinton agar with 2% glucose and 0.5 micro g of methylene blue per ml. The supplement may be added to the agar before autoclaving, or Mueller-Hinton agar plates may be flooded w...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Barry A,Bille J,Brown S,Ellis D,Meis JF,Rennie R,Rinaldi M,Rogers T,Traczewski M

更新日期：2004-04-01 00:00:00

abstract：:The Leishmania species cause a variety of human disease syndromes. Methods for diagnosis and species differentiation are insensitive and many require invasive sampling. Although quantitative PCR (qPCR) methods are reported for leishmania detection, no systematic method to quantify parasites and determine the species i...

journal_title：Journal of clinical microbiology

authors： Weirather JL,Jeronimo SM,Gautam S,Sundar S,Kang M,Kurtz MA,Haque R,Schriefer A,Talhari S,Carvalho EM,Donelson JE,Wilson ME

更新日期：2011-11-01 00:00:00

abstract：:New variants of the influenza A(H1N1)pdm09 and A(H3N2) viruses were detected in Taiwan between 2012 and 2013. Some of these variants were not detected in clinical specimens using a common real-time reverse transcription-PCR (RT-PCR) assay that targeted the conserved regions of the viral matrix (M) genes. An analysis o...

journal_title：Journal of clinical microbiology

authors： Yang JR,Kuo CY,Huang HY,Wu FT,Huang YL,Cheng CY,Su YT,Chang FY,Wu HS,Liu MT

更新日期：2014-01-01 00:00:00

abstract：:Cryptococcus neoformans var. gattii is a causative agent of cryptococcosis and is thought to have a specific ecological association with a number of Eucalyptus species in Australia. However, the role that the tree plays in the life cycle of the fungus and the nature of the infectious propagule are not well understood....

journal_title：Journal of clinical microbiology

authors： Halliday CL,Carter DA

更新日期：2003-02-01 00:00:00

abstract：:During the course of a molecular epidemiology study of mechanisms of antibiotic resistance in the area served by our hospital (516,000 inhabitants), we isolated the gene encoding CTX-M-14 beta-lactamase. Thirty clinical strains (27 Escherichia coli and 3 Klebsiella pneumoniae isolates) with a phenotype of extended-spe...

journal_title：Journal of clinical microbiology

authors： Bou G,Cartelle M,Tomas M,Canle D,Molina F,Moure R,Eiros JM,Guerrero A

更新日期：2002-11-01 00:00:00

abstract：:A toxic shock syndrome toxin 1 (TSST-1) antibody-binding protein produced by an ovine-associated strain of Staphylococcus aureus was examined. The protein showed total identity to TSST-1 by immunodiffusion analysis. Western blots (immunoblots) of proteins separated by isoelectric focusing revealed that the TSST-1 anti...

journal_title：Journal of clinical microbiology

authors： Ho G,Campbell WH,Carlson E

更新日期：1989-01-01 00:00:00

abstract：:Enzyme-linked immunosorbent assays were developed for the quantitation of anti-reovirus immunoglobulin G (IgG), IgA, and IgM in serum. The assays were specific, sensitive, and reproducible and measured antibodies to the reovirus group antigen. They should permit diagnosis of recent reovirus infection and longitudinal ...

journal_title：Journal of clinical microbiology

authors： Richardson SC,Bishop RF,Smith AL

更新日期：1988-09-01 00:00:00

abstract：:Cryptococcus gattii emerged in North America in 1999 as a human and veterinary pathogen on Vancouver Island, British Columbia. The emergent subtype, VGIIa, and the closely related subtype VGIIb can now be found in the United States in Washington, Oregon, and California. We performed multilocus sequence typing and anti...

journal_title：Journal of clinical microbiology

authors： Iqbal N,DeBess EE,Wohrle R,Sun B,Nett RJ,Ahlquist AM,Chiller T,Lockhart SR,Cryptococcus gattii Public Health Working Group.

更新日期：2010-02-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) based on the detection of specific antibodies in serum are commonly used for the diagnosis of visceral leishmaniasis (VL). Several commercial kits are available, and some of them allow the use of whole-blood samples instead of serum. An RDT is much more user-friendly for blood samples tha...

journal_title：Journal of clinical microbiology

authors： Kumar D,Khanal B,Tiwary P,Mudavath SL,Tiwary NK,Singh R,Koirala K,Boelaert M,Rijal S,Sundar S

更新日期：2013-12-01 00:00:00