Definition of false-positive reactions in screening for hepatitis C virus antibodies.

abstract：:A recombinant protein (p41-G) of an antigenic region of flagellin was used in a standard and amplified enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Borrelia burgdorferi, the causative agent of Lyme borreliosis. Comparable sensitivities (88 to 94%) were noted when sera from 17 persons who had eryth...

journal_title：Journal of clinical microbiology

authors： Magnarelli LA,Fikrig E,Berland R,Anderson JF,Flavell RA

更新日期：1992-12-01 00:00:00

abstract：:Candida samples were taken over a period of 2 years from 54 human immunodeficiency virus (HIV)-positive asymptomatic subjects to evaluate changes in yeast carriage, intensity of carriage, and genotype over time. Overall, we found that HIV-positive patients with CD4(+)-cell counts of between 200 and 400/microl had sign...

journal_title：Journal of clinical microbiology

authors： Vargas KG,Joly S

更新日期：2002-02-01 00:00:00

abstract：:An immunofluorescent-antibody test was developed for rapid detection of Pseudomonas aeruginosa in blood cultures. The test uses a murine monoclonal antibody specific for all strains of P. aeruginosa. In initial tests, bright uniform immunofluorescence signals were seen when each of the 17 international serotypes, as w...

journal_title：Journal of clinical microbiology

authors： Counts GW,Schwartz RW,Ulness BK,Hamilton DJ,Rosok MJ,Cunningham MD,Tam MR,Darveau RP

更新日期：1988-06-01 00:00:00

abstract：:The performance of the Amplified Mycobacterium Tuberculosis Direct (AMTD) test (Gen-Probe Inc., San Diego, Calif.) was assessed in a large tertiary care mycobacteriology laboratory. Both acid-fast smear-positive and smear-negative respiratory and nonrespiratory clinical specimens were analyzed. From February 1998 to 4...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Miller DR,Schneider PS,Roberts GD

更新日期：2002-05-01 00:00:00

abstract：:A method for the rapid detection of verotoxin-producing Escherichia coli in stool samples by PCR was evaluated. Verotoxin-1 and verotoxin-2 genes in DNA extracted directly from stool samples were amplified with oligonucleotide primers. Stools spiked with control organisms, E. coli C600 (H19B) (verotoxin-1) or E. coli ...

journal_title：Journal of clinical microbiology

authors： Ramotar K,Waldhart B,Church D,Szumski R,Louie TJ

更新日期：1995-03-01 00:00:00

abstract：:Bordetella pertussis is a Gram-negative bacterium that causes respiratory infections in humans. Ongoing molecular surveillance of B. pertussis acellular vaccine (aP) antigens is critical for understanding the interaction between evolutionary pressures, disease pathogenesis, and vaccine effectiveness. Methods currently...

journal_title：Journal of clinical microbiology

authors： Marchand-Austin A,Tsang RSW,Guthrie JL,Ma JH,Lim GH,Crowcroft NS,Deeks SL,Farrell DJ,Jamieson FB

更新日期：2017-05-01 00:00:00

abstract：:Anyplex STI-7 is a new molecular kit that detects seven sexually transmitted pathogens. Among 202 subjects screened for genital infection, 143 (70.4%) were diagnosed with at least one pathogen, in concordance with reference methods. In addition, the Anyplex STI-7 demonstrated coinfections, such as that with Ureaplasma...

journal_title：Journal of clinical microbiology

authors： Berçot B,Amarsy R,Goubard A,Aparicio C,Loeung HU,Segouin C,Gueret D,Jacquier H,Meunier F,Mougari F,Cambau E

更新日期：2015-03-01 00:00:00

abstract：:Owing to the difficulties in isolating the virus and the lack of routine surveillance, the clinical significance of human parainfluenza virus 4 (HPIV-4) is less well defined than that of the other human parainfluenza viruses. We describe the first outbreak of HPIV-4 infection in a developmental disabilities unit, invo...

journal_title：Journal of clinical microbiology

authors： Lau SK,To WK,Tse PW,Chan AK,Woo PC,Tsoi HW,Leung AF,Li KS,Chan PK,Lim WW,Yung RW,Chan KH,Yuen KY

更新日期：2005-09-01 00:00:00

abstract：:Meningococcal disease is normally suspected on clinical grounds and is confirmed by isolation of Neisseria meningitidis bacteria from blood or cerebrospinal fluid or, more recently, by serology or PCR of cerebrospinal fluid. Achieving confirmation of a clinical diagnosis of meningococcal disease has become more diffic...

journal_title：Journal of clinical microbiology

authors： Newcombe J,Cartwright K,Palmer WH,McFadden J

更新日期：1996-07-01 00:00:00

abstract：:Chlamydia pneumoniae is an important human respiratory pathogen. Classification of C. pneumoniae isolates into distinguishable serovars or genotypes has not yet been reported. To determine whether antigenic or molecular variants among C. pneumoniae isolates exist, six strains were studied via immunoblot analysis and D...

journal_title：Journal of clinical microbiology

authors： Jantos CA,Heck S,Roggendorf R,Sen-Gupta M,Hegemann JH

更新日期：1997-03-01 00:00:00

abstract：:The results of PCR-based molecular typing of Helicobacter pylori strains by restriction fragment length polymorphism analysis of a 1, 161-bp nucleotide sequence of the midregion of the vacA gene are reported. A total of 48 H. pylori strains isolated from gastric biopsy specimens obtained from 18 patients with peptic u...

journal_title：Journal of clinical microbiology

authors： Donati M,Storni E,D'Apote L,Moreno S,Tucci A,Poli L,Cevenini R

更新日期：1999-04-01 00:00:00

abstract：:Klebsiella pneumoniae carbapenemases (KPCs) are considered a serious threat to antibiotic therapy, as they confer resistance to carbapenems, which are used to treat extended-spectrum beta-lactamase (ESBL)-producing bacteria. Here, we describe the development and evaluation of a DNA microarray for the detection and gen...

journal_title：Journal of clinical microbiology

authors： Peter H,Berggrav K,Thomas P,Pfeifer Y,Witte W,Templeton K,Bachmann TT

更新日期：2012-12-01 00:00:00

abstract：:The Vitek MS v2.0 matrix-assisted laser desorption ionization-time of flight mass spectrometry system accurately distinguished Streptococcus pneumoniae from nonpneumococcal S. mitis group species. Only 1 of 116 nonpneumococcal isolates (<1%) was misidentified as S. pneumoniae. None of 95 pneumococcal isolates was misi...

journal_title：Journal of clinical microbiology

authors： Branda JA,Markham RP,Garner CD,Rychert JA,Ferraro MJ

更新日期：2013-09-01 00:00:00

abstract：:A rapid and accurate method to identify the species and antibiotic resistance of Candida spp. in blood is vital to increase the survival rates of patients with bloodstream infections. However, the extremely low levels of Candida spp. in blood make rapid diagnosis by standard blood culture difficult. In this study, we ...

journal_title：Journal of clinical microbiology

authors： Chen XL,Zheng H,Li WG,Zhong YH,Chen XP,Lu JX

更新日期：2020-03-25 00:00:00

abstract：:Viral hemorrhagic fevers, because of their high mortality rates, the lack of medical countermeasures, and their potential use as instruments of bioterrorism, pose a significant threat to the developed and the developing areas of the world. The key to preventing the spread of these diseases is early and accurate detect...

journal_title：Journal of clinical microbiology

authors： Satterly NG,Voorhees MA,Ames AD,Schoepp RJ

更新日期：2016-12-28 00:00:00

abstract：:Antibodies to human papilloma virus (HPV) type 16 (HPV-16) E6 and E7 proteins in serum are markers for HPV-associated invasive cervical carcinoma. We compared two assays, a radioimmunoprecipitation assay with in vitro-translated HPV-16 E6 and E7 proteins and an enzyme-linked immunosorbent assay (ELISA) with E6 and E7 ...

journal_title：Journal of clinical microbiology

authors： Sun Y,Shah KV,Müller M,Muñoz N,Bosch XF,Viscidi RP

更新日期：1994-09-01 00:00:00

abstract：:Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

journal_title：Journal of clinical microbiology

authors： Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

更新日期：2011-09-01 00:00:00

abstract：:Mueller-Hinton (MH) agar media from various commercial sources, either supplemented or not supplemented with 5% sheep blood, were studied to determine their effect on disk diffusion susceptibility testing results obtained with 90 strains of group D streptococci and four cephalosporins. The cephalosporins investigated ...

journal_title：Journal of clinical microbiology

authors： Sahm DF,Baker CN,Jones RN,Thornsberry C

更新日期：1983-10-01 00:00:00

abstract：:Bordetella spp. cause respiratory tract diseases in warm-blooded animals. Only Bordetella bronchiseptica has been reported to cause bacteremia in humans, and this rare infection usually occurs with pneumonia in immunocompromised patients. We describe "Bordetella hinzii" bacteremia in an AIDS patient without a respirat...

journal_title：Journal of clinical microbiology

authors： Cookson BT,Vandamme P,Carlson LC,Larson AM,Sheffield JV,Kersters K,Spach DH

更新日期：1994-10-01 00:00:00

abstract：:The prevalence of tick-borne infections has been steadily increasing in both number and geographic distribution in the United States and abroad. This increase, in conjunction with the continued recognition of novel pathogens transmitted by ticks, has made accurate diagnosis of these infections challenging. Mainstay se...

journal_title：Journal of clinical microbiology

authors： Buchan BW,Jobe DA,Mashock M,Gerstbrein D,Faron ML,Ledeboer NA,Callister SM

更新日期：2019-10-23 00:00:00

abstract：:The effects of selective media and atmosphere of incubation on the isolation of group A beta-hemolytic streptococci were evaluated. Sheep blood agar medium with gentamicin (2.5 or 5.0 mug/ml) was inferior to antibiotic-free sheep blood agar medium. This resulted from the partial restriction of group A streptococcal gr...

journal_title：Journal of clinical microbiology

authors： Murray PR,Wold AD,Schreck CA,Washington JA II

更新日期：1976-07-01 00:00:00

abstract：:Four slightly yellow-pigmented, alpha-hemolytic, gram-negative coccobacilli, three from wound specimens and one from multiple blood cultures of a patient with endocarditis, were identified as Neisseria elongata subsp. glycolytica on the basis of their overall biochemical and genetic similarities to this subspecies. Th...

journal_title：Journal of clinical microbiology

authors： Andersen BM,Weyant RS,Steigerwalt AG,Moss CW,Hollis DG,Weaver RE,Ashford D,Brenner DJ

更新日期：1995-01-01 00:00:00

abstract：:A large pool of refined endotoxin was prepared from Escherichia coli O113 by extraction with hot acqueous phenol. It was characterized chemically and biologically and will be available for a reference standard designated as reference endotoxin EC. ...

journal_title：Journal of clinical microbiology

authors： Rudbach JA,Akiya FI,Elin RJ,Hochstein HD,Luoma MK,Milner EC,Milner KC,Thomas KR

更新日期：1976-01-01 00:00:00

abstract：:To our knowledge, no data are available on whether the microbial species composition and abundance sampled with self-collected vaginal swabs are comparable to those of swabs collected by clinicians. Twenty healthy women were recruited to the study during a routine gynecological visit. Eligible women were between 18 an...

journal_title：Journal of clinical microbiology

authors： Forney LJ,Gajer P,Williams CJ,Schneider GM,Koenig SS,McCulle SL,Karlebach S,Brotman RM,Davis CC,Ault K,Ravel J

更新日期：2010-05-01 00:00:00

abstract：:Urinary tract infections caused by staphylococci are usually attributed to Staphylococcus epidermidis or S. saprophyticus. The case study reported here describes a persistent urinary tract infection caused by S. haemolyticus in a 38-year-old male whose infection was ultimately resolved through the use of the antibioti...

journal_title：Journal of clinical microbiology

authors： Gunn BA,Davis CE Jr

更新日期：1988-05-01 00:00:00

abstract：:A disk method for detecting hippurate hydrolysis by Campylobacter spp. was evaluated and compared with the conventional tube test used at the Centers for Disease Control (CDC) (G.K. Morris and C.M. Patton, p. 302-308, in E.H. Lennette, A. Balows, W.J. Hausler, Jr., and H.J. Shadomy, ed., Manual of Clinical Microbiolog...

journal_title：Journal of clinical microbiology

authors： Cacho JB,Aguirre PM,Hernanz A,Velasco AC

更新日期：1989-02-01 00:00:00

abstract：:A 24-year-old man sustained a crush injury to the tibia, which subsequently became infected with Saksenaea vasiformis. He was treated with debridement and a free myocutaneous flap, but amputation was necessary because of mycotic osteomyelitis. S. vasiformis was recovered and identified on the basis of its characterist...

journal_title：Journal of clinical microbiology

authors： Pierce PF,Wood MB,Roberts GD,Fitzgerald RH Jr,Robertson C,Edson RS

更新日期：1987-05-01 00:00:00

abstract：:We have investigated 677 Shiga toxin-producing Escherichia coli (STEC) strains from humans to determine their serotypes, virulence genes, and clinical signs in patients. Six different Shiga toxin types (1, 1c, 2, 2c, 2d, and 2e) were distributed in the STEC strains. Intimin (eae) genes were present in 62.6% of the str...

journal_title：Journal of clinical microbiology

authors： Beutin L,Krause G,Zimmermann S,Kaulfuss S,Gleier K

更新日期：2004-03-01 00:00:00

abstract：:A screening assay for phospholipase C using a chromogenic substrate incorporated into agar medium is described. The assay directly visualizes phospholipase C activity of mycoplasma lysates and membranes on agar plates, or the activity may be measured by spectrophotometry. The results from the assay confirm the presenc...

journal_title：Journal of clinical microbiology

authors： De Silva NS,Quinn PA

更新日期：1987-04-01 00:00:00

abstract：:The mold Aureobasidium pullulans was isolated on several nutrient media from a splenic abscess in a patient with disseminated lymphoma. Examination of stained smears and paraffin sections revealed fungal structures characteristic of this organism. This is the first reported association of A. pullulans with an opportun...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Martinez JA,Kemna ME

更新日期：1986-05-01 00:00:00