Comparison of self-collected and physician-collected vaginal swabs for microbiome analysis.

abstract：:False sensitivities were occasionally encountered in antibiotic-free chambers of the Autobac I system. This unusual phenomenon occurred randomly among gram-negative bacilli and appears to be colistin-related. ...

journal_title：Journal of clinical microbiology

authors： Duckenfield L,Wadke M,Ashton JK

更新日期：1980-10-01 00:00:00

abstract：:Eleven isolates representing five distinct outbreaks of pneumococcal conjunctivitis were examined for phenotypic and genetic characteristics. None of the strains possessed capsules, and all strains were susceptible to optochin, bile soluble, and Gen-Probe AccuProbe test positive. All 11 isolates were confirmed as Stre...

journal_title：Journal of clinical microbiology

authors： Carvalho MG,Steigerwalt AG,Thompson T,Jackson D,Facklam RR

更新日期：2003-09-01 00:00:00

abstract：:Aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. Sampling was by syringe aspiration. Strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. Randomly...

journal_title：Journal of clinical microbiology

authors： Williams BL,McCann GF,Schoenknecht FD

更新日期：1983-10-01 00:00:00

abstract：:We have developed a novel high-throughput PCR-ligase detection reaction-capillary electrophoresis (PCR-LDR-CE) assay for the multiplexed identification of 20 blood-borne pathogens (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Enterococcus faecium, Listeria monocytogenes, S...

journal_title：Journal of clinical microbiology

authors： Pingle MR,Granger K,Feinberg P,Shatsky R,Sterling B,Rundell M,Spitzer E,Larone D,Golightly L,Barany F

更新日期：2007-06-01 00:00:00

abstract：:Whole-house surveillance for healthcare-associated infection is no longer the recommended practice because of the large personnel time investment required. We developed a computer-based tracking system using microbiologic data as an aid in detecting potential outbreaks of healthcare-associated infections on a hospital...

journal_title：Journal of clinical microbiology

authors： Hacek DM,Cordell RL,Noskin GA,Peterson LR

更新日期：2004-03-01 00:00:00

abstract：:Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Epidemiological studies of GBS infections require comprehensive typing systems that provide informa...

journal_title：Journal of clinical microbiology

authors： Sun Y,Kong F,Zhao Z,Gilbert GL

更新日期：2005-09-01 00:00:00

abstract：:A fecal PCR assay for detection of Helicobacter infections in laboratory rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not require euthanasia of ro...

journal_title：Journal of clinical microbiology

authors： Beckwith CS,Franklin CL,Hook RR Jr,Besch-Williford CL,Riley LK

更新日期：1997-06-01 00:00:00

abstract：:Here we describe the first reported case of Nocardia beijingensis infection in the United States, made rarer by its presence in an immunocompetent patient. ...

journal_title：Journal of clinical microbiology

authors： Crozier JA,Andhavarapu S,Brumble LM,Sher T

更新日期：2014-07-01 00:00:00

abstract：:Species of coagulase-negative staphylococci isolated from urine specimens submitted from both inpatients and outpatients to the clinical microbiology laboratory of a teaching hospital were identified with a biotyping system, with species then correlated by clinical features and antimicrobial susceptibility. Of 145 iso...

journal_title：Journal of clinical microbiology

authors： Nicolle LE,Hoban SA,Harding GK

更新日期：1983-02-01 00:00:00

abstract：:Thirty acquired immunodeficiency syndrome patients with mycobacterial bacteremia documented by Du Pont Isolator (Du Pont Co., Wilmington, Del.) blood cultures underwent microscopic examination of buffy coat blood smears. Of 30 patients, 14 were culture positive for Mycobacterium avium-Mycobacterium intracellulare comp...

journal_title：Journal of clinical microbiology

authors： Eng RH,Bishburg E,Smith SM,Mangia A

更新日期：1989-04-01 00:00:00

abstract：:A procedure using the Smart Cycler instrument and a fluorescence quencher (FQ) probe for the specific identification of Mycobacterium tuberculosis complex (MTB) was used to detect organisms in 366 acid-fast bacillus smear-positive respiratory specimens. It was compared to culture and the AMPLICOR M. tuberculosis PCR t...

journal_title：Journal of clinical microbiology

authors： Cleary TJ,Roudel G,Casillas O,Miller N

更新日期：2003-10-01 00:00:00

abstract：:Microarrays of virus-specific oligonucleotides may provide a method of screening samples for the presence or absence of a large variety of viruses simultaneously. Influenza viruses are ideal for evaluating such microarrays because of their genetic and host diversity, and the availability of an extensive sequence datab...

journal_title：Journal of clinical microbiology

authors： Sengupta S,Onodera K,Lai A,Melcher U

更新日期：2003-10-01 00:00:00

abstract：:In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here...

journal_title：Journal of clinical microbiology

authors： Lodes MJ,Suciu D,Elliott M,Stover AG,Ross M,Caraballo M,Dix K,Crye J,Webby RJ,Lyon WJ,Danley DL,McShea A

更新日期：2006-04-01 00:00:00

abstract：:A 2000-2001 U.S. Haemophilus influenzae surveillance study (n = 1,434) detected nine (0.6%) beta-lactamase-negative and ampicillin-resistant (BLNAR) isolates collected from two different hospitals. The MICs of ampicillin for all nine isolates were 4 microg/ml, with results being reproducible; and all nine isolates wer...

journal_title：Journal of clinical microbiology

authors： Karlowsky JA,Critchley IA,Blosser-Middleton RS,Karginova EA,Jones ME,Thornsberry C,Sahm DF

更新日期：2002-03-01 00:00:00

abstract：:Pulmonary infection with Pseudomonas aeruginosa is a major cause of morbidity and mortality in cystic fibrosis (CF) patients. P. aeruginosa toxin is one of several proposed virulence factors which may be responsible for chronic P. aeruginosa infections in these patients. With a highly specific, sensitive, and quantita...

journal_title：Journal of clinical microbiology

authors： Cukor G,Blacklow NR,Nowak NA,Rich CM,Braverman LE,Fischer RA

更新日期：1983-09-01 00:00:00

abstract：:The incidence of antimicrobial-resistant Neisseria gonorrhoeae continues to rise in Canada; however, antimicrobial resistance data are lacking for approximately 70% of gonorrhea infections that are diagnosed directly from clinical specimens by nucleic acid amplification tests (NAATs). We developed a molecular assay fo...

journal_title：Journal of clinical microbiology

authors： Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

更新日期：2015-07-01 00:00:00

abstract：:The Vidas Chlamydia test (CHL) is an automated enzyme-linked immunofluorescence assay for the detection of Chlamydia trachomatis. Positive and equivocal results are confirmed with a blocking assay. A mouse monoclonal antibody directed against the chlamydial lipopolysaccharides was used for the test. The CHL assay is w...

journal_title：Journal of clinical microbiology

authors： Schachter J,Jones RB,Butler RC,Rice B,Brooks D,Van der Pol B,Gray M,Moncada J

更新日期：1997-08-01 00:00:00

abstract：:In accordance with recent WHO recommendations, this study evaluates the sensitivities of PCR and microscopy for fine-needle aspiration (FNA) versus techniques involving swabs and punch biopsy specimens and suggests that FNA can replace punch biopsies for nonulcerative lesions and may serve as an alternative for ulcera...

journal_title：Journal of clinical microbiology

authors： Herbinger KH,Beissner M,Huber K,Awua-Boateng NY,Nitschke J,Thompson W,Klutse E,Agbenorku P,Assiobo A,Piten E,Wiedemann F,Fleischmann E,Helfrich K,Adjei O,Löscher T,Bretzel G

更新日期：2010-10-01 00:00:00

abstract：:A Swedish bovine and a Dutch human phage set for coagulase-negative staphylococci were used to phage type coagulase-negative staphylococci isolated from bovine milk from Minnesota dairy herds. A comparison was also made of the deoxyribonuclease activity of coagulase-negative staphylococci isolated from bovine milk in ...

journal_title：Journal of clinical microbiology

authors： Holmberg O,Lyon RH,Price D,Farnsworth RJ

更新日期：1979-09-01 00:00:00

abstract：:A production model of the semi-automated PASCO MIC-ID system (PASCO Laboratories, Wheat Ridge, Colo.) was evaluated with 122 groups or species of gram-negative bacilli, which included typical (499 cultures) and atypical (37 cultures) strains of fermenters and nonfermenters. The PASCO system identified 90.9% of 536 cul...

journal_title：Journal of clinical microbiology

authors： Rhoden DL,Schable B,Smith PB

更新日期：1987-12-01 00:00:00

abstract：:The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more freque...

journal_title：Journal of clinical microbiology

authors： Russo C,Tortoli E,Menichella D

更新日期：2006-02-01 00:00:00

abstract：:In 1997 and 1998, H3N2 influenza A viruses emerged among pigs in North America. Genetic analyses of the H3N2 isolates demonstrated that they had distinctly different genotypes. The most commonly isolated viruses in the United States have a triple-reassortant genotype, with the hemagglutinin, neuraminidase, and PB1 pol...

journal_title：Journal of clinical microbiology

authors： Landolt GA,Karasin AI,Phillips L,Olsen CW

更新日期：2003-05-01 00:00:00

abstract：:A human astrovirus (HAstV) strain from Kenya was characterized by nucleotide sequence analysis. Sequences from open reading frame 1a (ORF1a) clustered with genotype 6/7, those from ORF1b clustered with genotype 3, and those from ORF2 clustered with genotype 2. A recombination point in the ORF1b-ORF2 junction was ident...

journal_title：Journal of clinical microbiology

authors： Wolfaardt M,Kiulia NM,Mwenda JM,Taylor MB

更新日期：2011-02-01 00:00:00

abstract：:Saksenaea is a monotypic genus belonging to the order Mucorales and capable of producing severe human infections. Through a polyphasic study based on analysis of the sequences of the internal transcribed spacer (ITS) region, domains D1 and D2 of the 28S rRNA gene, and the elongation factor 1α (EF-1α) gene, as well as ...

journal_title：Journal of clinical microbiology

authors： Alvarez E,Garcia-Hermoso D,Sutton DA,Cano JF,Stchigel AM,Hoinard D,Fothergill AW,Rinaldi MG,Dromer F,Guarro J

更新日期：2010-12-01 00:00:00

abstract：:Counterimmunoelectrophoresis was compared with electron microscopy for detection of human reovirus-like agent in fecal specimens. Both tests gave very similar results. ...

journal_title：Journal of clinical microbiology

authors： Spence L,Fauvel M,Petro R,Bloch S

更新日期：1977-02-01 00:00:00

abstract：:In order to increase the application potential of the direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in human serum samples, we developed an antigen based on stained and freeze-dried Leishmania donovani promastigotes. We describe here the evaluation of the performance of the DAT based ...

journal_title：Journal of clinical microbiology

authors： Meredith SE,Kroon NC,Sondorp E,Seaman J,Goris MG,van Ingen CW,Oosting H,Schoone GJ,Terpstra WJ,Oskam L

更新日期：1995-07-01 00:00:00

abstract：:We compared the results obtained with six commercial MIC test systems (Etest, MicroScan, Phoenix, Sensititre, Vitek Legacy, and Vitek 2 systems) and three reference methods (agar dilution, disk diffusion, and vancomycin [VA] agar screen [VScr]) with the results obtained by the Clinical and Laboratory Standards Institu...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Anderson KF,Lonsway DR,Thompson A,McAllister SK,Limbago BM,Carey RB,Tenover FC,Patel JB

更新日期：2009-07-01 00:00:00

abstract：:Between 1998 and 2001, tissues from four captive white-tailed deer were observed to have histologic lesions of systemic lymphocytic vasculitis. These lesions suggested malignant catarrhal fever, although epizootic hemorrhagic disease and bluetongue were included in the differential diagnosis. Initial diagnostic effort...

journal_title：Journal of clinical microbiology

authors： Kleiboeker SB,Miller MA,Schommer SK,Ramos-Vara JA,Boucher M,Turnquist SE

更新日期：2002-04-01 00:00:00

abstract：:Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one comme...

journal_title：Journal of clinical microbiology

authors： Ye S,Lambert SB,Grimwood K,Roczo-Farkas S,Nimmo GR,Sloots TP,Kirkwood CD,Whiley DM

更新日期：2015-01-01 00:00:00

abstract：:Drug-resistant tuberculosis (TB) is a major threat to TB control worldwide. Globally, only 40% of the 340,000 notified TB patients estimated to have multidrug-resistant-TB (MDR-TB) were detected in 2015. This study was carried out to evaluate the utility of high-resolution melt curve analysis (HRM) for the rapid and d...

journal_title：Journal of clinical microbiology

authors： Anthwal D,Gupta RK,Bhalla M,Bhatnagar S,Tyagi JS,Haldar S

更新日期：2017-06-01 00:00:00