Use of semiconductor-based oligonucleotide microarrays for influenza a virus subtype identification and sequencing.

abstract：:Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are orbiviruses that infect both livestock and wild ruminants. Antigenic cross-reactivity between BTV and EHDV often results in serologic misdiagnosis. Competitive enzyme-linked immunosorbent assays (c-ELISAs) show increased sensitivity and specific...

journal_title：Journal of clinical microbiology

authors： Mecham JO,Wilson WC

更新日期：2004-02-01 00:00:00

abstract：:Microagglutination and micro-enzyme-linked immunosorbent assay (ELISA) tests with easily prepared, safe, heat-killed antigens for detecting antibodies to the legionnaires disease organism have been developed. A safranin-stained whole-cell antigen is used in the microagglutination test, and a simply prepared soluble an...

journal_title：Journal of clinical microbiology

authors： Farshy CE,Klein GC,Feeley JC

更新日期：1978-04-01 00:00:00

abstract：:Thirty-seven bacterial clones producing human recombinant monoclonal antibody Fab fragments (rFabs) reactive to herpes simplex virus (HSV) antigens were selected from a human combinatorial antibody library constructed in a phage-display vector by a panning procedure against an HSV lysate. Thirty-four of the HSV-specif...

journal_title：Journal of clinical microbiology

authors： Cattani P,Rossolini GM,Cresti S,Santangelo R,Burton DR,Williamson RA,Sanna PP,Fadda G

更新日期：1997-06-01 00:00:00

abstract：:Accurate species identification of enterococci has become important with the wide prevalence of acquired vancomycin resistance and the presence of less epidemiologically important, inherently vancomycin-resistant enterococci. Using a collection of enterococcal strains, we found that PCR amplification of the intergenic...

journal_title：Journal of clinical microbiology

authors： Tyrrell GJ,Bethune RN,Willey B,Low DE

更新日期：1997-05-01 00:00:00

abstract：:A simple, sensitive, and specific polymerase chain reaction (PCR) protocol for the detection of human immunodeficiency virus type 1 (HIV-1) is described. We have improved all three PCR steps: sample preparation, DNA amplification, and detection of the amplified product. Some of the improvements have been described pre...

journal_title：Journal of clinical microbiology

authors： Albert J,Fenyö EM

更新日期：1990-07-01 00:00:00

abstract：:Bacteroides forsythus has been associated with destructive adult periodontitis. Up to now, detailed analysis by classical means was hampered by the fastidious nature of the organism. There is hope that the application of molecular detection methods such as indirect immunofluorescence or in situ hybridization (ISH) wil...

journal_title：Journal of clinical microbiology

authors： Gersdorf H,Meissner A,Pelz K,Krekeler G,Göbel UB

更新日期：1993-04-01 00:00:00

abstract：:The recent Zaire Ebola virus (EBOV) outbreak in West Africa illustrates clearly the need for additional studies with humans and animals to elucidate the ecology of Ebola viruses (EBVs). In this study, we developed a serological assay based on the Luminex technology. Nine recombinant proteins representing different vir...

journal_title：Journal of clinical microbiology

authors： Ayouba A,Touré A,Butel C,Keita AK,Binetruy F,Sow MS,Foulongne V,Delaporte E,Peeters M

更新日期：2016-12-28 00:00:00

abstract：:Cefaclor is less stable than most cephalosporins in media at 35 degrees C. We demonstrated that the activity of cefaclor in Mueller-Hinton agar diminishes continuously at 4 degrees C, resulting in a loss of two-thirds of the activity within 21 days. We recommend that agar dilution plates for this cephalosporin be prep...

journal_title：Journal of clinical microbiology

authors： Surprenant AM,Preston DA

更新日期：1985-01-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did t...

journal_title：Journal of clinical microbiology

authors： Angamuthu R,Baskaran S,Gopal DR,Devarajan J,Kathaperumal K

更新日期：2012-03-01 00:00:00

abstract：:During the last 10 to 15 years, Aerococcus-like organisms have been isolated from urinary tract specimens from hospitalized patients at Bispebjerg Hospital, Copenhagen, Denmark, with signs of urinary tract infection, and a single strain has been deposited at the National Collection of Type Cultures (NCTC 12142). An ad...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Vibits H,Ursing J,Korner B

更新日期：1991-05-01 00:00:00

abstract：:Of 216 fecal and intestinal samples collected from nursing or weaned diarrheic pigs in the United States and Canada, 57 were identified as group A rotavirus positive by RNA electrophoresis and silver staining. Fifty-seven and 52 rotavirus-positive samples were analyzed by hybridization with Gottfried and OSU PCR-deriv...

journal_title：Journal of clinical microbiology

authors： Rosen BI,Parwani AV,Lopez S,Flores J,Saif LJ

更新日期：1994-02-01 00:00:00

abstract：:Nocardia isolations increased from 0.7 to 11.7/1,000 acid-fast bacillus and mycological cultures (P less than 0.000001). Only three isolations from one patient represented infection. Pseudoepidemic strain identity was confirmed by DNA fingerprinting; the isolate causing infection was distinct. The end of the pseudoepi...

journal_title：Journal of clinical microbiology

authors： Patterson JE,Chapin-Robertson K,Waycott S,Farrel P,McGeer A,McNeil MM,Edberg SC

更新日期：1992-05-01 00:00:00

abstract：:Formalin-fixed, purified reticulate bodies (RB) of Chlamydia trachomatis immunotype C/TW-3/OT were used as a serological test antigen in the microimmunofluorescence test. The sensitivity and specificity of the RB antigen were compared to elementary bodies (EB) used as antigens in the detection of C. trachomatis antibo...

journal_title：Journal of clinical microbiology

authors： Yong EC,Chinn JS,Caldwell HD,Kuo CC

更新日期：1979-09-01 00:00:00

abstract：:The BBL Crystal system (Becton Dickinson Microbiology Systems, Cockeysville, Md.) is a miniaturized bacterial identification method employing modified conventional and chromogenic substrates. Two products are currently available, the Rapid Stool/Enteric ID Kit and the Enteric/Nonfermenter ID Kit, each comprising thirt...

journal_title：Journal of clinical microbiology

authors： Holmes B,Costas M,Thaker T,Stevens M

更新日期：1994-09-01 00:00:00

abstract：:The present report describes an analysis of two virulence genes of Helicobacter pylori. Parts of the cagA gene, as well as parts from the signal (s) and middle (m) regions of the mosaic vacA gene, were amplified with biotin-labelled PCR primers and the products were subsequently analyzed by a single-step reverse hybri...

journal_title：Journal of clinical microbiology

authors： van Doorn LJ,Figueiredo C,Rossau R,Jannes G,van Asbroek M,Sousa JC,Carneiro F,Quint WG

更新日期：1998-05-01 00:00:00

abstract：:Failure to demonstrate immunoglobulin M (IgM) antibodies by indirect immunofluorescence (IgM-IFA) in sera from some patients with acute acquired toxoplasmosis has recently been attributed to an inhibitory effect of high titers of IgG antibodies in these sera (Pyndiah et al. J. Clin. Microbiol. 9:170-174, 1979). To con...

journal_title：Journal of clinical microbiology

authors： Filice GA,Yeager AS,Remington JS

更新日期：1980-09-01 00:00:00

abstract：:The Lyme disease-associated spirochete, Borrelia burgdorferi, is maintained in enzootic cycles involving Ixodes ticks and small mammals. Previous studies demonstrated that B. burgdorferi expresses outer surface protein A (OspA) but not OspC when residing in the midgut of unfed ticks. However, after ticks feed on blood...

journal_title：Journal of clinical microbiology

authors： Schwan TG,Piesman J

更新日期：2000-01-01 00:00:00

abstract：:Parasitologic confirmation of cutaneous leishmaniasis is obligatory before chemotherapy can be considered. Direct microscopic examination of scrapings taken from indurated borders of ulcers has been routinely used as primary method of diagnosis. In this report we compared the sensitivity of examination of dermal scrap...

journal_title：Journal of clinical microbiology

authors： Ramírez JR,Agudelo S,Muskus C,Alzate JF,Berberich C,Barker D,Velez ID

更新日期：2000-10-01 00:00:00

abstract：:A rapid (2.5 h) direct enzyme immunoassay (EIA) for Clostridium difficile toxin A was developed for clinical use. Specimen centrifugation and filtration were not required. The EIA detected toxin A levels in patient stool as low as 20 pg (2 ng/ml of stool). The test was 5,000 times more sensitive for toxin A than it wa...

journal_title：Journal of clinical microbiology

authors： DiPersio JR,Varga FJ,Conwell DL,Kraft JA,Kozak KJ,Willis DH

更新日期：1991-12-01 00:00:00

abstract：:Accurate and rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is needed to screen MRSA carriers and improve treatment. The current widely used duplex PCR methods are not able to differentiate MRSA from coexisting methicillin-susceptible S. aureus (MSSA) or other methicillin-resistant staphylo...

journal_title：Journal of clinical microbiology

authors： Luo J,Li J,Yang H,Yu J,Wei H

更新日期：2017-10-01 00:00:00

abstract：:In Brazil, the most common etiological agent of American tegumentary leishmaniasis is Leishmania (Viannia) braziliensis. In general, diagnostic techniques envisage the visualization of the parasite, but that technique has a low sensitivity. The main purpose of the present work was to evaluate the PCR as a routine tool...

journal_title：Journal of clinical microbiology

authors： Pirmez C,da Silva Trajano V,Paes-Oliveira Neto M,da-Cruz AM,Gonçalves-da-Costa SC,Catanho M,Degrave W,Fernandes O

更新日期：1999-06-01 00:00:00

abstract：:In the last decade, the global emergence of carbapenem resistance in Enterobacteriaceae has posed great concern to public health. Data concerning the role of environmental contamination in the dissemination of carbapenem-resistant Enterobacteriaceae (CRE) are currently lacking. Here, we aimed to examine the extent of ...

journal_title：Journal of clinical microbiology

authors： Lerner A,Adler A,Abu-Hanna J,Meitus I,Navon-Venezia S,Carmeli Y

更新日期：2013-01-01 00:00:00

abstract：:Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), a...

journal_title：Journal of clinical microbiology

authors： Imaz MS,Comini MA,Zerbini E,Sequeira MD,Latini O,Claus JD,Singh M

更新日期：2004-02-01 00:00:00

abstract：:A total of 525 cerebrospinal fluid (CSF) samples submitted during the 2007 and 2008 enteroviral seasons were included in a study to determine the prevalence of and potential risk factors for invalid Cepheid GeneXpert enterovirus assay (GXEA) results, as well as possible solutions for the problem. The invalid GXEA resu...

journal_title：Journal of clinical microbiology

authors： Sefers SE,Raymer AK,Kilby JT,Persing DH,Tang YW

更新日期：2009-09-01 00:00:00

abstract：:Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization...

journal_title：Journal of clinical microbiology

authors： Kusunoki S,Ezaki T,Tamesada M,Hatanaka Y,Asano K,Hashimoto Y,Yabuuchi E

更新日期：1991-08-01 00:00:00

abstract：:A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems cons...

journal_title：Journal of clinical microbiology

authors： Suzuki N,Nakano Y,Yoshida A,Yamashita Y,Kiyoura Y

更新日期：2004-08-01 00:00:00

abstract：:We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing. ...

journal_title：Journal of clinical microbiology

authors： Ahmed AO,Desplaces N,Leonard P,Goldstein F,De Hoog S,Verbrugh H,van Belkum A

更新日期：2003-12-01 00:00:00

abstract：:Standard repetitive extragenic palindromic (REP)-PCR, enterobacterial repetitive intergenic consensus-PCR, and Salmonella enteritidis repetitive element-PCR methods for bacterial strain typing were performed with DNA extracted by boiling members of each of the currently recognized species of human viridans group strep...

journal_title：Journal of clinical microbiology

authors： Alam S,Brailsford SR,Whiley RA,Beighton D

更新日期：1999-09-01 00:00:00

abstract：:Actinobacillus pleuropneumoniae strains of serotypes 1 and 9 were studied for their serological properties by means of agglutination, coagglutination (CoA), indirect hemagglutination (IHA), Co-IHA, ring precipitation (RP), and immunodiffusion (ID) tests. Particulate and soluble antigens of unheated and heat-treated ba...

journal_title：Journal of clinical microbiology

authors： Mittal KR

更新日期：1990-03-01 00:00:00

abstract：:A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyp...

journal_title：Journal of clinical microbiology

authors： Diancourt L,Passet V,Verhoef J,Grimont PA,Brisse S

更新日期：2005-08-01 00:00:00