Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens.

abstract：:Two national surveys were conducted to describe the incidence and prevalence of Enterobacter aerogenes in 21 Belgian hospitals in 1996 and 1997 and to characterize the genotypic diversity and the antimicrobial resistance profiles of clinical strains of E. aerogenes isolated from hospitalized patients in Belgium in 199...

journal_title：Journal of clinical microbiology

authors： De Gheldre Y,Struelens MJ,Glupczynski Y,De Mol P,Maes N,Nonhoff C,Chetoui H,Sion C,Ronveaux O,Vaneechoutte M,Groupement pour le Dépistage, l'Etude et la Prevention des Infections Hospitalières (GDEPIH-GOSPIZ).

更新日期：2001-03-01 00:00:00

abstract：:Of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, Butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of Campylobacter jejuni from cecal contents of chickens and chicken livers. It allowed the retrieval of 1 CFU...

journal_title：Journal of clinical microbiology

authors： Barot MS,Bokkenheuser VD

更新日期：1984-07-01 00:00:00

abstract：:Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10 Mycobacterium tuberculosis samples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpe...

journal_title：Journal of clinical microbiology

authors： Mokaddas E,Ahmad S,Eldeen HS,Al-Mutairi N

更新日期：2015-04-01 00:00:00

abstract：:Vibrio metschnikovii and Vibrio gazogenes are two new Vibrio species that have been little studied. Thirteen strains of V. metschnikovii were highly related to the type strain, NCTC 8443, by DNA-DNA hybridization. Relatedness values were 83 to 90% at 60 degrees C and 75 to 84% at the more stringent 75 degrees C. Diver...

journal_title：Journal of clinical microbiology

authors： Farmer JJ 3rd,Hickman-Brenner FW,Fanning GR,Gordon CM,Brenner DJ

更新日期：1988-10-01 00:00:00

abstract：:In Brazil, Salmonella enterica serovar Infantis resistant to various antimicrobials, including cephalosporins, has been identified as an etiological agent of severe gastroenteritis in hospitalized children since 1994. In this study, 35 serovar Infantis strains, isolated from children admitted to four different Rio de ...

journal_title：Journal of clinical microbiology

authors： Fonseca EL,Mykytczuk OL,Asensi MD,Reis EM,Ferraz LR,Paula FL,Ng LK,Rodrigues DP

更新日期：2006-08-01 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis causes Johne's disease, a systemic infection and chronic inflammation of the intestine that affects many species, including primates. Infection is widespread in livestock, and human populations are exposed. Johne's disease is associated with immune dysregulation, with involv...

journal_title：Journal of clinical microbiology

authors： Scanu AM,Bull TJ,Cannas S,Sanderson JD,Sechi LA,Dettori G,Zanetti S,Hermon-Taylor J

更新日期：2007-12-01 00:00:00

abstract：:We report a case of pneumonia with a fatal outcome caused by a nonhemolytic strain of Streptococcus pyogenes. This strain was isolated in pure growth from blood cultures and was fully identified biochemically. Such strains will be difficult to recognize and isolate from sites with heavy growth of normal flora. This ph...

journal_title：Journal of clinical microbiology

authors： Taylor MB,Barkham T

更新日期：2002-06-01 00:00:00

abstract：:Neisseria meningitidis represents a pathogen of great public health importance in both developed and developing countries. Resistance to some antimicrobial agents used either for therapy of invasive infections or for prophylaxis of case contacts has long been recognized, although specific guidelines for susceptibility...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Crawford SA,Fiebelkorn KR

更新日期：2005-07-01 00:00:00

abstract：:Eleven isolates of Aeromonas species from human stool cultures were found to produce enterotoxin activity as determined by assay of culture filtrates in rabbit intestinal loops and rabbit skin and on adrenal Y1 cells. Hemolysin(s) and a cytotoxic protein were found to interfere in all three assay systems but could be ...

journal_title：Journal of clinical microbiology

authors： Ljungh A,Popoff M,Wadstrom T

更新日期：1977-08-01 00:00:00

abstract：:It has been difficult to obtain pure Pneumocystis carinii antigen either from cultures or from infected lungs for use in producing a specific antibody against P. carinii. This report describes an approach toward producing a monoclonal antibody that bypasses the antigen purification steps. P. carinii infection was deve...

journal_title：Journal of clinical microbiology

authors： Lee CH,Bolinger CD,Bartlett MS,Kohler RB,Wilde CE 3rd,Smith JW

更新日期：1986-03-01 00:00:00

abstract：:PCR was used to amplify a 537-bp region of an Ehrlichia ewingii gene encoding a homologue of the 28-kDa major antigenic protein (P28) of Ehrlichia chaffeensis. The E. ewingii p28 gene homologue was amplified from DNA extracted from whole blood obtained from four humans and one canine with confirmed cases of infection....

journal_title：Journal of clinical microbiology

authors： Gusa AA,Buller RS,Storch GA,Huycke MM,Machado LJ,Slater LN,Stockham SL,Massung RF

更新日期：2001-11-01 00:00:00

abstract：:Pneumocystis jirovecii pneumonia (PCP) is an opportunistic infection with airborne transmission and remains a major cause of respiratory illness among immunocompromised individuals. In recent years, several outbreaks of PCP, occurring mostly in kidney transplant recipients, have been reported. Currently, multilocus se...

journal_title：Journal of clinical microbiology

authors： Maitte C,Leterrier M,Le Pape P,Miegeville M,Morio F

更新日期：2013-09-01 00:00:00

abstract：:The detection of porphyrins produced from omega-aminolevulinic acid was evaluated for use in speciation of the Haemophilus. Two hundred and forty-eight clinical isolates of nonhemolytic Haemophilus were tested concurrently for porphyrin production and for X and V requirements. The porphyrin production test was more ra...

journal_title：Journal of clinical microbiology

authors： Lund ME,Blazevic DJ

更新日期：1977-02-01 00:00:00

abstract：:Ten cultivable equine rotavirus isolates, two of North American, six of British, and two of Irish origin, were compared with standard rotavirus strains and with each other by cross neutralization, neutralization with a panel of monoclonal antibodies (MAbs), hybridization to a simian rotavirus (SA-11) VP7 gene probe, a...

journal_title：Journal of clinical microbiology

authors： Browning GF,Chalmers RM,Fitzgerald TA,Snodgrass DR

更新日期：1992-02-01 00:00:00

abstract：:The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more freque...

journal_title：Journal of clinical microbiology

authors： Russo C,Tortoli E,Menichella D

更新日期：2006-02-01 00:00:00

abstract：:Standard repetitive extragenic palindromic (REP)-PCR, enterobacterial repetitive intergenic consensus-PCR, and Salmonella enteritidis repetitive element-PCR methods for bacterial strain typing were performed with DNA extracted by boiling members of each of the currently recognized species of human viridans group strep...

journal_title：Journal of clinical microbiology

authors： Alam S,Brailsford SR,Whiley RA,Beighton D

更新日期：1999-09-01 00:00:00

abstract：:Two multiplex PCR enzyme immunoassays (PCR-EIAs) were developed for Staphylococcus aureus exotoxin gene screening as an alternative to the conventional biological assays, which depend on detectable amounts of toxins produced. One set of oligonucleotide primers and probes was designed to search for enterotoxin A to E g...

journal_title：Journal of clinical microbiology

authors： Becker K,Roth R,Peters G

更新日期：1998-09-01 00:00:00

abstract：:Leifsonia aquatica is an aquatic bacterium that is typically found in environmental water habitats. Infections due to L. aquatica are rare and commonly catheter associated in immunocompromised patients. We report the first case of an acute septicemia caused by L. aquatica in a healthy immunocompetent host after cryope...

journal_title：Journal of clinical microbiology

authors： Han L,Lei JE,Wang X,Guo LT,Kang QY,He L,Xu JR

更新日期：2013-11-01 00:00:00

abstract：:We standardized and assessed the performance of an in-house microtiter assay for determining the susceptibilities of Mycobacterium tuberculosis clinical isolates to isoniazid based on mycobacteriophage amplification technology. Seventy isolates (43 resistant and 27 sensitive according to the BACTEC 460 radiometric met...

journal_title：Journal of clinical microbiology

authors： Galí N,Domínguez J,Blanco S,Prat C,Alcaide F,Coll P,Ausina V

更新日期：2006-01-01 00:00:00

abstract：:We hypothesized that diversion of the first milliliter of venipuncture blood-the initial specimen diversion technique (ISDT)-would eliminate incompletely sterilized fragments of skin from the culture specimen and significantly reduce our blood culture contamination rate (R). We studied our hypothesis prospectively beg...

journal_title：Journal of clinical microbiology

authors： Patton RG,Schmitt T

更新日期：2010-12-01 00:00:00

abstract：:Polymyxins are relied upon for the treatment of carbapenem-resistant Gram-negative bacterial infections, but polymyxin resistance is increasing. Only broth microdilution is recommended for polymyxin susceptibility testing, but this method is impractical for most clinical microbiology laboratories. An article in this i...

journal_title：Journal of clinical microbiology

authors： Satlin MJ

更新日期：2019-01-30 00:00:00

abstract：:Sixty rotavirus-positive stool specimens from children with diarrhea were classified into G and P genotypes. G typing was done by PCR and then by hybridization with G type-specific (G1 to G4) oligonucleotide probes, whereas nested PCR was performed for P typing. Thirty-nine samples could be classified into both G and ...

journal_title：Journal of clinical microbiology

authors： Husain M,Seth P,Dar L,Broor S

更新日期：1996-06-01 00:00:00

abstract：:Murex hybrid capture DNA assay (HCS) is a solution hybridization antibody capture assay for detection and quantitation of cytomegalovirus (CMV) DNA in leukocytes. To determine whether CMV HCS is sensitive enough to initiate and monitor antiviral therapy after allogeneic stem cell transplantation (SCT), 51 consecutive ...

journal_title：Journal of clinical microbiology

authors： Hebart H,Gamer D,Loeffler J,Mueller C,Sinzger C,Jahn G,Bader P,Klingebiel T,Kanz L,Einsele H

更新日期：1998-05-01 00:00:00

abstract：:A screening assay for phospholipase C using a chromogenic substrate incorporated into agar medium is described. The assay directly visualizes phospholipase C activity of mycoplasma lysates and membranes on agar plates, or the activity may be measured by spectrophotometry. The results from the assay confirm the presenc...

journal_title：Journal of clinical microbiology

authors： De Silva NS,Quinn PA

更新日期：1987-04-01 00:00:00

abstract：:Performance in proficiency testing (PT) schemes is an objective measure of a laboratory's best performance. We examined the performance of participants in two parasitology PT schemes in South Africa from 2004 through 2010. The average rates of acceptable scores over the period were 58% and 66% for the stool and blood ...

journal_title：Journal of clinical microbiology

authors： Poonsamy B,Dini L,Frean J

更新日期：2012-10-01 00:00:00

abstract：:We compared the Difco ESP 384 blood culture system with the pediatric Septi-Chek system for the detection of bloodstream infections in pediatric patients. A total of 10,762 blood culture sets included an ESP aerobic bottle and a Septi-Chek bottle. From these cultures, a total of 278 organisms classified as probable pa...

journal_title：Journal of clinical microbiology

authors： Welby PL,Keller DS,Storch GA

更新日期：1995-05-01 00:00:00

abstract：:Infection of calves with bovine herpesvirus 1 (BHV-1) results in transient immunosuppression that may lead to bacterium-induced pneumonia and, occasionally, death. Although sensory neurons in the trigeminal ganglia (TG) are the primary site of BHV-1 latency, viral genomes are detected in the tonsils of latently infect...

journal_title：Journal of clinical microbiology

authors： Perez S,Inman M,Doster A,Jones C

更新日期：2005-01-01 00:00:00

abstract：:Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. ...

journal_title：Journal of clinical microbiology

authors： Arko RJ,Wong KH,Peacock WL

更新日期：1979-04-01 00:00:00

abstract：:We have used a polymerase chain reaction (PCR) to examine cervical cancer biopsy specimens and pelvic lymph nodes for the presence of human papillomavirus type 16 (HPV 16) DNA. Of the 75 cervical specimens tested, 36 (48%) were positive for HPV 16 in the PCR. A total of 65 pelvic lymph nodes removed during radical sur...

journal_title：Journal of clinical microbiology

authors： Czeglédy J,Póka R,Veress G,Gergely L

更新日期：1992-01-01 00:00:00

abstract：:Immunoblots were used to study the immunoglobulin G response to Borrelia burgdorferi in experimentally and naturally exposed dogs. Adsorption studies confirmed that the antibodies were specific for B. burgdorferi. Experimentally exposed dogs were asymptomatic. Naturally exposed dogs included both asymptomatic animals ...

journal_title：Journal of clinical microbiology

authors： Greene RT,Walker RL,Nicholson WL,Heidner HW,Levine JF,Burgess EC,Wyand M,Breitschwerdt EB,Berkhoff HA

更新日期：1988-04-01 00:00:00