Discordance between Xpert MTB/RIF assay and Bactec MGIT 960 Culture System for detection of rifampin-resistant Mycobacterium tuberculosis isolates in a country with a low tuberculosis (TB) incidence.

abstract：:Collecting whole blood on filter paper simplifies the processing, transport, and storage of specimens used for the diagnosis of human immunodeficiency virus type 1 (HIV-1) and other tests. Specimens may be collected in tropical or rural areas with minimal facilities for handling specimens. To compare simulated tropica...

journal_title：Journal of clinical microbiology

authors： Mitchell C,Jennings C,Brambilla D,Aldrovandi G,Amedee AM,Beck I,Bremer JW,Coombs R,Decker D,Fiscus S,Fitzgibbon J,Luzuriaga K,Moye J,Palumbo P,Reichelderfer P,Somasundaran M,Stevens W,Frenkel L,Dried Blood Spot Workin

更新日期：2008-09-01 00:00:00

abstract：:We developed a strategy to determine the clinical impact associated with errors in clinical microbiology testing. Over a 9-month period, we used a sequential three-stage method to prospectively evaluate 480 consecutive corrected microbiology laboratory reports. The three stages were physician review of the corrected r...

journal_title：Journal of clinical microbiology

authors： Yuan S,Astion ML,Schapiro J,Limaye AP

更新日期：2005-05-01 00:00:00

abstract：:A combined clinical and molecular epidemiologic analysis of 46 strains of Clostridium difficile, including 16 nosocomial isolates from one ward (outbreak ward) plus 17 other nosocomial isolates and 13 community-acquired isolates, was performed. HindIII digests of total cellular DNA were analyzed by restriction enzyme ...

journal_title：Journal of clinical microbiology

authors： Kristjánsson M,Samore MH,Gerding DN,DeGirolami PC,Bettin KM,Karchmer AW,Arbeit RD

更新日期：1994-08-01 00:00:00

abstract：:Toxin-specific enzyme immunoassays, cytotoxicity assays, and PCR were used to analyze 48 toxin A-negative, toxin B-positive Clostridium difficile isolates from various geographical sites around the world. All the isolates were negative by the TOX-A TEST and positive by the TOX A/B TEST. A deletion of approximately 1.7...

journal_title：Journal of clinical microbiology

authors： Moncrief JS,Zheng L,Neville LM,Lyerly DM

更新日期：2000-08-01 00:00:00

abstract：:Haemophilus influenzae type b (Hib) is, in contrast to non-type b H. influenzae, associated with severe invasive disease, such as meningitis and epiglottitis, in small children. To date, accurate H. influenzae capsule typing requires PCR, a time-consuming and cumbersome method. Matrix-assisted laser desorption ionizat...

journal_title：Journal of clinical microbiology

authors： Månsson V,Resman F,Kostrzewa M,Nilson B,Riesbeck K

更新日期：2015-07-01 00:00:00

abstract：:Paecilomyces variotii is a commonly occurring species in air and food, but it is also associated with many types of human infections and is among the emerging causative agents of opportunistic mycoses in immunocompromised hosts. Paecilomyces can cause hyalohyphomycosis, and two species, Paecilomyces lilacinus and P. v...

journal_title：Journal of clinical microbiology

authors： Houbraken J,Verweij PE,Rijs AJ,Borman AM,Samson RA

更新日期：2010-08-01 00:00:00

abstract：:In this study we describe a multiplex PCR assay for the detection of nine clinically relevant antibiotic resistance genes of Staphylococcus aureus. Conditions were optimized to amplify fragments of mecA (encoding methicillin resistance), aacA-aphD (aminoglycoside resistance), tetK, tetM (tetracycline resistance), erm(...

journal_title：Journal of clinical microbiology

authors： Strommenger B,Kettlitz C,Werner G,Witte W

更新日期：2003-09-01 00:00:00

abstract：:The Mycobacterium tuberculosis complex includes the four species M. tuberculosis, M. bovis, M. africanum, and M. microti. We sequenced 13 M. tuberculosis complex strains in the 16S-to-23S rDNA internal transcribed spacer (ITS). The ITS has a high rate of nucleotide substitution. Previous reports found three nucleotide...

journal_title：Journal of clinical microbiology

authors： Frothingham R,Hills HG,Wilson KH

更新日期：1994-07-01 00:00:00

abstract：:We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species le...

journal_title：Journal of clinical microbiology

authors： Won H,Rothman R,Ramachandran P,Hsieh YH,Kecojevic A,Carroll KC,Aird D,Gaydos C,Yang S

更新日期：2010-09-01 00:00:00

abstract：:The human immunodeficiency virus (HIV) was isolated from the blood of 192 of 410 seropositive individuals. Original isolations were made in peripheral blood mononuclear cell (PBMC) cultures, and only one-fifth of the HIV isolates could be adapted to replicate in continuous T-cell lines. Of the 192 HIV isolates, 42 had...

journal_title：Journal of clinical microbiology

authors： Bolton V,Pedersen NC,Higgins J,Jennings M,Carlson J

更新日期：1987-08-01 00:00:00

abstract：:Two extended-spectrum beta-lactamase (ESBL)-producing salmonella isolates, Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium, were analyzed. Both isolates produced the CTX-M-3 ESBL; however, their bla(CTX-M-3) genes were located on different plasmids. The serovar Typhimurium isolate a...

journal_title：Journal of clinical microbiology

authors： Baraniak A,Sadowy E,Hryniewicz W,Gniadkowski M

更新日期：2002-03-01 00:00:00

abstract：:Invasive aspergillosis (IA) is a life-threatening infection that mainly occurs in immunocompromised patients. Here, we compared the novel Aspergillus-specific galactomannoprotein (GP) enzyme-linked immunosorbent assay (ELISA) (Euroimmun Medizinische Labordiagnostika AG) to the established Platelia Aspergillus galactom...

journal_title：Journal of clinical microbiology

authors： Dichtl K,Seybold U,Ormanns S,Horns H,Wagener J

更新日期：2019-06-25 00:00:00

abstract：:Buruli ulcer (BU) is a necrotizing infection of skin and soft tissue caused by Mycobacterium ulcerans. In Australia, most cases of BU are linked to temperate, coastal Victoria and tropical, northern Queensland, and strains from these regions are distinguishable by variable-number tandem repeat (VNTR) typing. We presen...

journal_title：Journal of clinical microbiology

authors： Lavender CJ,Globan M,Johnson PD,Charles PG,Jenkin GA,Ghosh N,Clark BM,Martinello M,Fyfe JA

更新日期：2012-11-01 00:00:00

abstract：:Mitochondrial cytochrome b (cyt b) genes of 42 strains representing 23 species of the genus Trichosporon were partially sequenced to determine their molecular phylogenetic relationships. Almost half of the 22 strains investigated (from 11 different species) contained introns in their sequences. Analysis of a 396-bp co...

journal_title：Journal of clinical microbiology

authors： Biswas SK,Wang L,Yokoyama K,Nishimura K

更新日期：2005-10-01 00:00:00

abstract：:Four methods for the detection of Trichomonas vaginalis in vaginal secretions from 88 symptomatic patients were compared: wet-mount examination, Kupferberg liquid medium, Hirsch charcoal agar, and the Papanicolaou smear. Hirsch diphasic slants and Kupferberg medium did not significantly differ in sensitivity from dire...

journal_title：Journal of clinical microbiology

authors： Thomason JL,Gelbart SM,Sobun JF,Schulien MB,Hamilton PR

更新日期：1988-09-01 00:00:00

abstract：:Molecular typing of 246 Staphylococcus aureus isolates from unselected patients in Thailand showed that 10 (4.1%) were actually Staphylococcus argenteus. Contrary to the suggestion that S. argenteus is less virulent than S. aureus, we demonstrated comparable rates of morbidity, death, and health care-associated infect...

journal_title：Journal of clinical microbiology

authors： Thaipadungpanit J,Amornchai P,Nickerson EK,Wongsuvan G,Wuthiekanun V,Limmathurotsakul D,Peacock SJ

更新日期：2015-03-01 00:00:00

abstract：:Approximately 15 to 35% of patients with a first episode of Clostridium difficile-associated diarrhea relapse within 2 months. Between 1994 and 1997, strains from 93 hospitalized patients with C. difficile recurrences were fingerprinted by using both serotyping and PCR-ribotyping. The results showed that 48.4% of clin...

journal_title：Journal of clinical microbiology

authors： Barbut F,Richard A,Hamadi K,Chomette V,Burghoffer B,Petit JC

更新日期：2000-06-01 00:00:00

abstract：:Data are limited for the performance of enzyme immunoassays for the detection of Chlamydia trachomatis in conjunctival and nasopharyngeal specimens from infants. The only available data are for one assay, Chlamydiazyme (Abbott Diagnostics). The purpose of this study was to compare a new enzyme immunoassay, Pathfinder ...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Roblin PM,Gelling M,Worku M

更新日期：1990-08-01 00:00:00

abstract：:The Gen-Probe Amplified Mycobacterium tuberculosis direct test is widely used in the diagnosis of tuberculosis. Specimens may contain amplification inhibitors, potentially leading to false-negative results if unrecognized. We report a failure to detect inhibition, despite adherence to the inhibition testing guidelines...

journal_title：Journal of clinical microbiology

authors： Kim JY,Ferraro MJ,Branda JA

更新日期：2009-09-01 00:00:00

abstract：:Staphylococcus aureus produced an inhibitory factor that suppressed slime formation by Psuudomonas aeruginosa but had little effect on the growth of the organism. The inhibitory factor was not found in broth cultures but could be extracted from cultures grown on solid agar. The inhibitory factor moderately inhibited g...

journal_title：Journal of clinical microbiology

authors： Shibl AM,Al-Sowaygh IA

更新日期：1980-02-01 00:00:00

abstract：:A fundamental role for the endosymbiotic bacteria Wolbachia pipientis in the pathogenesis of Dirofilaria immitis infections has emerged in recent years. Diagnostic opportunities arising from this breakthrough have not yet been fully exploited. This study was aimed at developing conventional and real-time PCR assays to...

journal_title：Journal of clinical microbiology

authors： Turba ME,Zambon E,Zannoni A,Russo S,Gentilini F

更新日期：2012-08-01 00:00:00

abstract：:The aim of this study was to describe the frequency of positive Aspergillus tests in COVID-19 patients and investigate the association between COVID-19 and a positive Aspergillus test result. We compared the proportion of positive Aspergillus tests in COVID-19 patients admitted to the ICU for > 24 hours with two contr...

journal_title：Journal of clinical microbiology

authors： Yusuf E,Vonk A,van den Akker JPC,Bode L,Sips GJ,Rijnders BJA,de Steenwinkel J,Verkaik NJ,Vogel M,van der Eerden M,van Westreenen M

更新日期：2020-12-04 00:00:00

abstract：:We tested 606 specimens for cytomegalovirus infection. Fifty-two specimens were positive by conventional culture versus forty-three by pre-cytopathic effect detection. Twenty-nine specimens were positive by both methods. Because of toxicity, specimens should be set up on both conventional and shell vial culture system...

journal_title：Journal of clinical microbiology

authors： Rabella N,Drew WL

更新日期：1990-04-01 00:00:00

abstract：:An enzymatic profile of 20 strains of Pseudomonas maltophilia was undertaken with conventional plate tests, API ZYM, and 4-methylumbelliferyl-conjugated substrates. All strains produced DNase, RNase, arbutinase, esterases and lipases, mucinase, acid and alkaline phosphatases, alkaline pyrophosphate diesterase, phospho...

journal_title：Journal of clinical microbiology

authors： O'Brien M,Davis GH

更新日期：1982-09-01 00:00:00

abstract：:Inconsistent quality control results in disk diffusion testing of cefaclor, cefamandole, cefonicid, and cefuroxime with Haemophilus influenzae ATCC 49247 and Haemophilus test medium (HTM) prompted a search for an alternative control strain that would provide more reliable results. A five-laboratory study was conducted...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Barry AL,Doern GV,Ferraro MJ,Murray PR

更新日期：1992-08-01 00:00:00

abstract：:A combined method of the Limulus amebocyte lysate (LAL) test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigenicity of coagulogen, a major protein in LAL, was lost when LAL reacted with endotoxin as shown by immunoblotting. Determination of ...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Koch C

更新日期：1988-08-01 00:00:00

abstract：:The utility of combined 16S rRNA (rrs) gene restriction fragment length polymorphism and plasmid profiles to differentiate between and within Campylobacter upsaliensis of human and canine origin was examined. Fourteen distinct rrs gene restriction fragment length polymorphs consisting of bands sized between 1.9 and 4....

journal_title：Journal of clinical microbiology

authors： Stanley J,Jones C,Burnens A,Owen RJ

更新日期：1994-07-01 00:00:00

abstract：:The genetic composition and antibiotic sensitivities of 50 clinical isolates of Staphylococcus aureus obtained from various clinics in the Republic of Georgia were characterized. S. aureus strains ATCC 700699 and ATCC 29737 were included as reference standards in all analyses. All 52 strains had identical 16S rRNA pro...

journal_title：Journal of clinical microbiology

authors： Revazishvili T,Bakanidze L,Gomelauri T,Zhgenti E,Chanturia G,Kekelidze M,Rajanna C,Kreger A,Sulakvelidze A

更新日期：2006-10-01 00:00:00

abstract：:Anthopsis deltoidea was found to be the cause of an olecranon bursitis in a 79-year-old golfer. Serial histological sections of the olecranon bursa showed faintly stained, brown-walled, septate, hyphal elements in the centers of the necrotic debris. The combination of bursectomy and flucytosine treatment cured the inf...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Droller DD

更新日期：1984-08-01 00:00:00

abstract：:We attempted to find a relationship between the microbiological properties of bloodstream isolates of methicillin-resistant Staphylococcus aureus (MRSA) and the efficacy of vancomycin in the treatment of bacteremia. Vancomycin susceptibility testing was performed, and bactericidal activity was determined for 30 isolat...

journal_title：Journal of clinical microbiology

authors： Sakoulas G,Moise-Broder PA,Schentag J,Forrest A,Moellering RC Jr,Eliopoulos GM

更新日期：2004-06-01 00:00:00