Comparison of four methods to detect Trichomonas vaginalis.

abstract：:A 42-plex clustered regularly interspaced short palindromic repeat (CRISPR)-based typing technique (spoligotyping) was recently developed at the French National Reference Center for Legionella. It allows the subtyping of the Legionella pneumophila sequence type 1/Paris pulsotype. In this report, we present the transfe...

journal_title：Journal of clinical microbiology

authors： Gomgnimbou MK,Ginevra C,Peron-Cane C,Versapuech M,Refrégier G,Jacotin N,Sola C,Jarraud S

更新日期：2014-07-01 00:00:00

abstract：:To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplifi...

journal_title：Journal of clinical microbiology

authors： Pietilä J,He Q,Oksi J,Viljanen MK

更新日期：2000-07-01 00:00:00

abstract：:Sapporo-like viruses (SLVs) are associated with acute gastroenteritis in humans. Due to a limited supply of available reagents for diagnosis, little is known about the incidence and pathogenicity of these viruses. We have developed a first-generation generic reverse transcriptase (RT) PCR assay based on a single prime...

journal_title：Journal of clinical microbiology

authors： Vinjé J,Deijl H,van der Heide R,Lewis D,Hedlund KO,Svensson L,Koopmans MP

更新日期：2000-02-01 00:00:00

abstract：:Molecular techniques such as spa typing and multilocus sequence typing use DNA sequence data for differentiating Staphylococcus aureus isolates. Although spa typing is capable of detecting both genetic micro- and macrovariation, it has less discriminatory power than the more labor-intensive pulsed-field gel electropho...

journal_title：Journal of clinical microbiology

authors： Koreen L,Ramaswamy SV,Naidich S,Koreen IV,Graff GR,Graviss EA,Kreiswirth BN

更新日期：2005-08-01 00:00:00

abstract：:Streptococcus pneumoniae is a major cause of bacteremia, meningitis, pneumonia, sinusitis, and acute otitis media in children. Although optochin susceptibility, bile solubility, and Quellung testing are the standards for identifying and differentiating pneumococci, there are several reports of nontypeable pneumococci ...

journal_title：Journal of clinical microbiology

authors： Ing J,Mason EO,Kaplan SL,Lamberth LB,Revell PA,Luna RA,Hulten KG

更新日期：2012-04-01 00:00:00

abstract：:Whole HIV-1 genome sequences are pivotal for large-scale studies of inter- and intrahost evolution, including the acquisition of drug resistance mutations. The ability to rapidly and cost-effectively generate large numbers of HIV-1 genome sequences from different populations and geographical locations and determine th...

journal_title：Journal of clinical microbiology

authors： Gall A,Ferns B,Morris C,Watson S,Cotten M,Robinson M,Berry N,Pillay D,Kellam P

更新日期：2012-12-01 00:00:00

abstract：:During 5.5 years of a study in Washington, D.C., hospitalizations of children for rotavirus gastroenteritis tended to be more common after a month of cold or dry weather than after a corresponding calendar month of warm or wet weather. Overall, there were 84% more (178 versus 97) inpatients with rotavirus gastroenteri...

journal_title：Journal of clinical microbiology

authors： Brandt CD,Kim HW,Rodriguez WJ,Arrobio JO,Jeffries BC,Parrott RH

更新日期：1982-09-01 00:00:00

abstract：:This collaborative three-center study evaluated NCCLS M38-A document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of Aspergillus nidulans, A. terreus, Bipolaris hawaiiensis, B. spicifera, Cladophialophora bantiana, Dactylaria constricta, Fusarium solani, Paec...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Chaturvedi V,Fothergill A,Rinaldi MG

更新日期：2002-10-01 00:00:00

abstract：:The Leifson staining method was used to diagnose Helicobacter pylori infection and was compared to histology, culture, and the rapid urease test (RUT). Histology gave the best sensitivity (98%), compared to Leifson staining (97%), culture (92%), and RUT (85%) (P < 0.005). Leifson staining is a sensitive, rapid, econom...

journal_title：Journal of clinical microbiology

authors： Piccolomini R,Di Bonaventura G,Neri M,Di Girolamo A,Catamo G,Pizzigallo E

更新日期：1999-01-01 00:00:00

abstract：:The virulence plasmid of Escherichia coli O157 strain EDL933 carries a 10-kb putative virulence gene designated toxB. Little is known about the distribution of this gene among E. coli O157 strains or its presence in other enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) strains. We developed PCR an...

journal_title：Journal of clinical microbiology

authors： Tozzoli R,Caprioli A,Morabito S

更新日期：2005-08-01 00:00:00

abstract：:DNA encoding two repeat units of 120-kDa protein of Ehrlichia chaffeensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an ind...

journal_title：Journal of clinical microbiology

authors： Yu XJ,Crocquet-Valdes P,Cullman LC,Walker DH

更新日期：1996-11-01 00:00:00

abstract：:We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species le...

journal_title：Journal of clinical microbiology

authors： Won H,Rothman R,Ramachandran P,Hsieh YH,Kecojevic A,Carroll KC,Aird D,Gaydos C,Yang S

更新日期：2010-09-01 00:00:00

abstract：:Over a 6-year period, 24 extended-spectrum beta-lactamase (ESBL)-producing isolates of Pseudomonas aeruginosa were collected from 18 patients living in a nursing home. These isolates had a delayed development of a red pigment and exhibited a similar antibiotype (resistance to all beta-lactams except for imipenem and t...

journal_title：Journal of clinical microbiology

authors： Dubois V,Arpin C,Noury P,Andre C,Coulange L,Quentin C

更新日期：2005-08-01 00:00:00

abstract：:A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. Th...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Tsunomori Y,Seki R

更新日期：2003-11-01 00:00:00

abstract：:The coagulation of Limulus amebocyte lysate (LAL) can be activated through two pathways, one initiated by endotoxin and the other by beta-glucans. The two pathways join at the step of activation of the proclotting enzyme. We report here that the endotoxin-activated pathway can be differentially inhibited by two method...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Buchardt O,Koch C

更新日期：1994-06-01 00:00:00

abstract：:A quantitative, indirect, fluorescence immunoassay (FIAX; Whittaker Bioproducts, Inc.) was compared with the conventional indirect fluorescent-antibody test for detection of serum antibody to Borrelia burgdorferi. FIAX correlated well with the indirect fluorescent-antibody test (r = 0.72). FIAX is a convenient and dep...

journal_title：Journal of clinical microbiology

authors： Pennell DR,Wand PJ,Schell RF

更新日期：1987-11-01 00:00:00

abstract：:Large hospital-based clinical laboratories must be prepared to rapidly investigate potential infectious disease outbreaks. To challenge the ability of our molecular diagnostics laboratory to use whole-genome sequencing in a potential outbreak scenario and identify impediments to these efforts, we studied 84 invasive s...

journal_title：Journal of clinical microbiology

authors： Olsen RJ,Fittipaldi N,Kachroo P,Sanson MA,Long SW,Como-Sabetti KJ,Valson C,Cantu C,Lynfield R,Van Beneden C,Beres SB,Musser JM

更新日期：2014-12-01 00:00:00

abstract：:Ventilator-associated pneumonia is frequently diagnosed with quantitative cultures of samples obtained by bronchoscopic techniques, a method associated with high costs and potential adverse effects. Quantitative cultures of endotracheal aspirates are easier and cheaper to obtain, and good correlations between the resu...

journal_title：Journal of clinical microbiology

authors： Bergmans DC,Bonten MJ,De Leeuw PW,Stobberingh EE

更新日期：1997-03-01 00:00:00

abstract：:The way in which the extensive use of highly active antiretroviral therapy (HAART) has influenced the incidence of visceral leishmaniasis (VL) among human immunodeficiency type 1 (HIV-1)-infected patients is not yet understood. The present study assessed whether the incidence of symptomatic VL in HIV-infected patients...

journal_title：Journal of clinical microbiology

authors： de La Rosa R,Pineda JA,Delgado J,Macías J,Morillas F,Mira JA,Sánchez-Quijano A,Leal M,Lissen E

更新日期：2002-03-01 00:00:00

abstract：:A novel panfungal PCR assay which detects the small-subunit rRNA gene sequence of the two major fungal organism groups was used to test whole-blood specimens obtained from a series of blood or bone marrow transplant recipients. The 580-bp PCR product was identified after amplification by panfungal primers and hybridiz...

journal_title：Journal of clinical microbiology

authors： Van Burik JA,Myerson D,Schreckhise RW,Bowden RA

更新日期：1998-05-01 00:00:00

abstract：:A new case of Campylobacter hyointestinalis-associated diarrhea in a human is reported. The medical history of the patient was significant for immunodeficiency because of an evolutive chronic myeloid leukemia. The diarrhea rapidly stopped after administration of oral erythromycin. No other enteropathogenic agent was f...

journal_title：Journal of clinical microbiology

authors： Minet J,Grosbois B,Megraud F

更新日期：1988-12-01 00:00:00

abstract：:A direct immunofluorescence assay (DFA) with a monoclonal antibody from Ortho Diagnostic Systems was compared with conventional cell culture for the rapid detection of varicella-zoster virus (VZV) in 140 dermal lesions from 133 patients. A total of 79 (56%) specimens were positive for VZV: 40 (51%) by DFA alone, 2 (3%...

journal_title：Journal of clinical microbiology

authors： Coffin SE,Hodinka RL

更新日期：1995-10-01 00:00:00

abstract：:Among 23 patients carrying methicillin-resistant Staphylococcus aureus (MRSA) in their anterior nares, 6 (26%) also carried methicillin-susceptible S. aureus (MSSA) as less prevalent flora. In 4 of the 6 patients, the MSSA was unrelated to prevalent MRSA, as determined by pulsed-field gel electrophoresis (PFGE), multi...

journal_title：Journal of clinical microbiology

authors： Boundy S,Zhao Q,Fairbanks C,Folgosa L,Climo M,Archer GL

更新日期：2012-02-01 00:00:00

abstract：:Pneumocystis jirovecii pneumonia (PCP) is an opportunistic infection with airborne transmission and remains a major cause of respiratory illness among immunocompromised individuals. In recent years, several outbreaks of PCP, occurring mostly in kidney transplant recipients, have been reported. Currently, multilocus se...

journal_title：Journal of clinical microbiology

authors： Maitte C,Leterrier M,Le Pape P,Miegeville M,Morio F

更新日期：2013-09-01 00:00:00

abstract：:This study evaluated a PCR method for the rapid detection of clinically significant genotypes of vancomycin-resistant enterococci (VRE) in nosocomial surveillance specimens. Detection of the vanA and vanB genes by multiplex PCR using 657 specimens that showed presumptive growth of VRE on bile esculin azide agar contai...

journal_title：Journal of clinical microbiology

authors： Jayaratne P,Rutherford C

更新日期：1999-06-01 00:00:00

abstract：:We present the first case of severe pneumonia possibly caused by Leptotrichia species with oral bacteria. This was found in a healthy but elderly subject whose bronchoalveolar lavage fluid was analyzed by 16S rRNA gene sequencing analysis. The combination of this method and microscopic observation provided useful info...

journal_title：Journal of clinical microbiology

authors： Kawanami T,Fukuda K,Yatera K,Kido T,Yoshii C,Taniguchi H,Kido M

更新日期：2009-02-01 00:00:00

abstract：:A 45-year-old man from Nepal with a 13-year history of polycystic kidney disease was diagnosed as suffering from chronic renal failure with end-stage renal disease. After receiving empirical antituberculosis treatment, he was treated with broad-spectrum antibiotics. A left nephrectomy was performed, and after 4 months...

journal_title：Journal of clinical microbiology

authors： Padhye AA,Verghese S,Ravichandran P,Balamurugan G,Hall L,Padmaja P,Fernandez MC

更新日期：2003-01-01 00:00:00

abstract：:Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10 Mycobacterium tuberculosis samples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpe...

journal_title：Journal of clinical microbiology

authors： Mokaddas E,Ahmad S,Eldeen HS,Al-Mutairi N

更新日期：2015-04-01 00:00:00

abstract：:We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the SmartCycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A+B and Binax NOW ...

journal_title：Journal of clinical microbiology

authors： Liao RS,Tomalty LL,Majury A,Zoutman DE

更新日期：2009-03-01 00:00:00

abstract：:Because self-collected vaginal swabs (VS) are potentially very useful for screening asymptomatic women for Chlamydia trachomatis infection, a multicenter study evaluated that specimen with nucleic acid amplification tests (NAATs). The objective was to determine whether VS are equal to Food and Drug Administration (FDA...

journal_title：Journal of clinical microbiology

authors： Schachter J,McCormack WM,Chernesky MA,Martin DH,Van Der Pol B,Rice PA,Hook EW 3rd,Stamm WE,Quinn TC,Chow JM

更新日期：2003-08-01 00:00:00