Characterization of nontypeable and atypical Streptococcus pneumoniae pediatric isolates from 1994 to 2010.

abstract：:Of 1,510 sputum samples examined from 1,016 patients with bronchopulmonary disorders, Nocardia asteroides was isolated from 67 samples by paraffin baiting, as compared with only 30 isolations by the conventional technique of culturing on Sabouraud dextrose agar. This higher efficacy of the paraffin bait technique was ...

journal_title：Journal of clinical microbiology

authors： Singh M,Sandhu RS,Randhawa HS

更新日期：1987-01-01 00:00:00

abstract：:Aberrant filamentous forms of Escherichia coli were observed on direct Gram stain of blood cultures from a patient being treated with the beta-lactam antibiotic cephalexin. After the institution of an alternative antibiotic regimen which included a different cell wall-active agent, E. coli of normal morphology was det...

journal_title：Journal of clinical microbiology

authors： Magnussen CR,Hruska JF

更新日期：1980-11-01 00:00:00

abstract：:Of 45 influenza virus strains (43 type A and 2 type B) detected in conventional tube cell cultures (average time, 4 days), 25 (56%) were detected by immunofluorescence in the shell vial assay 24 h postinoculation. The specific fluorescence produced should allow this procedure to be readily adapted by laboratories with...

journal_title：Journal of clinical microbiology

authors： Espy MJ,Smith TF,Harmon MW,Kendal AP

更新日期：1986-10-01 00:00:00

abstract：:Hepatitis C virus (HCV)-positive sera from 106 chronically infected patients which had previously been genotyped were characterized by serotyping. Genotypes were determined by a first-generation line probe assay (INNO-LiPA HCV) and by sequence analyses of the core, core-E1, and NS5B regions. HCV serotypes were determi...

journal_title：Journal of clinical microbiology

authors： van Doorn LJ,Kleter B,Pike I,Quint W

更新日期：1996-07-01 00:00:00

abstract：:Tracking the spread of noroviruses during outbreaks of gastroenteritis is hampered by the lack of sequence diversity in those regions of the genome chosen for virus detection and characterization. Sequence analysis of regions of the genes encoding the RNA-dependent RNA polymerase and the S domain of the capsid does no...

journal_title：Journal of clinical microbiology

authors： Xerry J,Gallimore CI,Iturriza-Gómara M,Allen DJ,Gray JJ

更新日期：2008-03-01 00:00:00

abstract：:Nasopharyngeal secretions from adults and children were obtained in Stockholm, Sweden, for routine diagnosis of influenza A virus, influenza B virus, respiratory syncytial (RS) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. The cells in nasoph...

journal_title：Journal of clinical microbiology

authors： Grandien M,Pettersson CA,Gardner PS,Linde A,Stanton A

更新日期：1985-11-01 00:00:00

abstract：:Detection of human cytomegalovirus (CMV) DNA in clinical specimens is considered a cornerstone in the diagnosis of CMV disease. The aim of this study was to evaluate a newly designed LightCycler-based quantitative CMV PCR. Specimens of human origin (n = 200) were tested using the LightCycler PCR, the quantitative COBA...

journal_title：Journal of clinical microbiology

authors： Schaade L,Kockelkorn P,Ritter K,Kleines M

更新日期：2000-11-01 00:00:00

abstract：:The first vector-borne Capnocytophaga canimorsus sepsis case is presented. An immunocompetent male who denied any contact with canines and who worked in a sawmill was bitten on his neck by a large pine weevil (Hylobius abietis L.; Coleoptera: Curculionidae). Bacteriological diagnosis was confirmed by 16S rRNA gene seq...

journal_title：Journal of clinical microbiology

authors： Tuuminen T,Viiri H,Vuorinen S

更新日期：2014-07-01 00:00:00

abstract：:Community-acquired bloodstream infections cause substantial morbidity and mortality worldwide, but microbiology capacity and surveillance limitations have challenged good descriptions of pathogen distribution in many regions, including Southeast Asia. Active surveillance for bloodstream infections has been conducted i...

journal_title：Journal of clinical microbiology

authors： Jorakate P,Higdon M,Kaewpan A,Makprasert S,Yuenprakhon S,Tawisaid K,Dejsirilert S,Whistler T,Baggett HC

更新日期：2015-03-01 00:00:00

abstract：:A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude bi...

journal_title：Journal of clinical microbiology

authors： Arita M

更新日期：2013-08-01 00:00:00

abstract：:Campylobacter rectus was isolated under routine anaerobic conditions (no additional hydrogen gas in the atmosphere) from an oral, nonperiodontal abscess from a patient with gastroesophageal adenocarcinoma. We report the first case of a palate abscess caused by C. rectus and review the literature and atmospheric requir...

journal_title：Journal of clinical microbiology

authors： Mahlen SD,Clarridge JE 3rd

更新日期：2009-03-01 00:00:00

abstract：:We evaluated the utility of the commercial Allplex genital ulcer real-time PCR multiplex assay for detecting Treponema pallidum, herpes simplex virus 1 (HSV-1) and 2 (HSV-2), and Chlamydia trachomatis serovar L (lymphogranuloma venereum [LGV]) DNA in mucosal and genital ulcers in the context of suspected syphilis. In ...

journal_title：Journal of clinical microbiology

authors： Grange PA,Jary A,Isnard C,Burrel S,Boutolleau D,Touati A,Bébéar C,Saule J,Martinet P,Robert JL,Moulene D,Vermersch-Langlin A,Benhaddou N,Janier M,Dupin N

更新日期：2021-01-21 00:00:00

abstract：:Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 +/- 9 days was significantly higher than that from sputa that were untreated and processed by the N-acetyl-L-cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subje...

journal_title：Journal of clinical microbiology

authors： Pardini M,Varaine F,Iona E,Arzumanian E,Checchi F,Oggioni MR,Orefici G,Fattorini L

更新日期：2005-01-01 00:00:00

abstract：:One of the major unanswered questions regarding the presence of Listeria monocytogenes in foods is how many cells must be ingested in order to cause illness. To answer this question, studies were undertaken by using Macaca fascicularis (cynomolgus monkey) as an animal model. Healthy nonhuman primates were dosed with v...

journal_title：Journal of clinical microbiology

authors： Farber JM,Daley E,Coates F,Beausoleil N,Fournier J

更新日期：1991-11-01 00:00:00

abstract：:Typical and atypical Campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. Some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as Campylobacter coli by DNA homology ...

journal_title：Journal of clinical microbiology

authors： Ng LK,Taylor DE,Stiles ME

更新日期：1988-03-01 00:00:00

abstract：:Neisseria meningitidis represents a pathogen of great public health importance in both developed and developing countries. Resistance to some antimicrobial agents used either for therapy of invasive infections or for prophylaxis of case contacts has long been recognized, although specific guidelines for susceptibility...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Crawford SA,Fiebelkorn KR

更新日期：2005-07-01 00:00:00

abstract：:The attenuated bacillus Calmette-Guérin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and other members of the M. tuberculosis complex by conventional methods. Recently, a genomic region designated RD1 was found to be pre...

journal_title：Journal of clinical microbiology

authors： Talbot EA,Williams DL,Frothingham R

更新日期：1997-03-01 00:00:00

abstract：:Neisseria gonorrhoeae is a common bacterial sexually transmitted infection (STI). Currently, there are limited data on the bacterial load in both men and women and on both genital and extragenital sites. Therefore, we quantified N. gonorrhoeae bacterial loads in a large population of women, heterosexual men, and men w...

journal_title：Journal of clinical microbiology

authors： van der Veer BMJW,Hoebe CJPA,Dukers-Muijrers NHTM,van Alphen LB,Wolffs PFG

更新日期：2020-10-21 00:00:00

abstract：:Polyomavirus BK (BKV) establishes latent infection in various human tissues, including the kidney. Reactivation following renal transplantation (RT) may cause BKV-associated nephropathy, leading to graft loss. BKV reactivation is often associated with extensive rearrangements in the BKV noncoding regulatory region (NC...

journal_title：Journal of clinical microbiology

authors： Perets TT,Silberstein I,Rubinov J,Sarid R,Mendelson E,Shulman LM

更新日期：2009-05-01 00:00:00

abstract：:Lower levels of performance of human papillomavirus (HPV) typing assays in studies using formalin-fixed paraffin-embedded (FFPE) tissue compared to those using exfoliated cervical cells have been reported. The interpretation of current studies is limited by bias in inclusion criteria, sample matching, and methods of c...

journal_title：Journal of clinical microbiology

authors： Dal Bello B,Spinillo A,Alberizzi P,Cesari S,Gardella B,Silini EM

更新日期：2009-07-01 00:00:00

abstract：:Cowdria ruminantium causes severe, often fatal disease in domestic ruminants, whereas wildlife species usually are not affected. Blood and bone marrow samples from healthy, free-ranging Zimbabwean ungulates were taken during translocation from areas harboring Amblyomma ticks and tested for the presence of C. ruminanti...

journal_title：Journal of clinical microbiology

authors： Kock ND,van Vliet AH,Charlton K,Jongejan F

更新日期：1995-09-01 00:00:00

abstract：:We evaluated the diagnostic value of antibody to Blastomyces dermatitidis A precipitin arcs for identifying B. dermatitidis cultures by the exoantigen technique with 88 isolates from the United States and Canada, 12 from Africa, and 1 each from India and Israel. In addition, we studied 190 mycelial-form isolates of ot...

journal_title：Journal of clinical microbiology

authors： Kaufman L,Standard PG,Weeks RJ,Padhye AA

更新日期：1983-07-01 00:00:00

abstract：:A multiplex real-time PCR assay was developed with a LightCycler instrument for detection of influenza viruses A and B and the human respiratory syncytial virus (HRSV). Detection of each viral product and of an internal control was based on determination of specific melting temperatures by the LightCycler software. Th...

journal_title：Journal of clinical microbiology

authors： Boivin G,Côté S,Déry P,De Serres G,Bergeron MG

更新日期：2004-01-01 00:00:00

abstract：:The classification of viruses is relevant to a number of scientific and clinical disciplines, including the practice of diagnostic virology. Here, we provide an update to our previous review of taxonomic changes for disease-causing viruses in humans and vertebrate animals, covering changes between 2018 and 2020. Recen...

journal_title：Journal of clinical microbiology

authors： Loeffelholz MJ,Fenwick BW

更新日期：2021-01-21 00:00:00

abstract：:The fluorescent amplified-fragment length polymorphism (FAFLP) assay potentially amplifies a unique set of genome fragments from each bacterial clone. It uses stringently hybridizing primers which carry a fluorescent label. Precise fragment sizing is achieved by the inclusion of an internal size standard in every lane...

journal_title：Journal of clinical microbiology

authors： Arnold C,Metherell L,Willshaw G,Maggs A,Stanley J

更新日期：1999-05-01 00:00:00

abstract：:Molecular genotyping of strains of Trichophyton rubrum and T. mentagrophytes from patients with onychomycosis of the toes was performed to ascertain whether the fungal genotype changes over the course of time as sequential samples were obtained from patients receiving antifungal therapy and during follow-up. Sixty-six...

journal_title：Journal of clinical microbiology

authors： Gupta AK,Kohli Y,Summerbell RC

更新日期：2001-09-01 00:00:00

abstract：:Adolescent children were surveyed for colonization with Mycoplasma hominis and Ureaplasma urealyticum by culturing urine specimens. Rates were compared between three study groups: (i) 397 children attending parochial schools, (ii) 293 children attending an adolescent clinic specializing in adjustment problems, and (ii...

journal_title：Journal of clinical microbiology

authors： Foy H,Kenny G,Bor E,Hammar S,Hickman R

更新日期：1975-09-01 00:00:00

abstract：:In this work, we describe the assembly of a synthetic gene coding for several antigenic determinants found in different Leishmania infantum antigens. Selected epitopes were derived from the ribosomal proteins LiP2a, LiP2b, and LiP0 and from the histone H2A. The resulting gene was overexpressed in Escherichia coli eith...

journal_title：Journal of clinical microbiology

authors： Soto M,Requena JM,Quijada L,Alonso C

更新日期：1998-01-01 00:00:00

abstract：:The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The t...

journal_title：Journal of clinical microbiology

authors： Janda WM,Zigler KL,Bradna JJ

更新日期：1987-02-01 00:00:00

abstract：:A competitive inhibition enzyme-linked immunosorbent assay (ELISA) based on a major surface protein 5 (MSP5) B-cell epitope conserved among Anaplasma species was used to detect goats infected with Anaplasma ovis. We examined strains of A. ovis isolated from goats in Kenya and demonstrated that MSP5 and the target B-ce...

journal_title：Journal of clinical microbiology

authors： Ndung'u LW,Aguirre C,Rurangirwa FR,McElwain TF,McGuire TC,Knowles DP,Palmer GH

更新日期：1995-03-01 00:00:00