Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses.

abstract：:The serological properties of six new pneumococcal capsular types are described. A table listing all 90 pneumococcal types and their cross-reactions is included. ...

journal_title：Journal of clinical microbiology

authors： Henrichsen J

更新日期：1995-10-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00

abstract：:An evaluation of the serotyping system for Proteus rettgeri has been performed through its application to 561 human isolates of this species. Of these, 545 were typable and could be separated into 58 O serotypes. A group of isolates from one hospital, and clinically implicated in cross-infections, was found to belong ...

journal_title：Journal of clinical microbiology

authors： Penner JL,Hinton NA,Hennessy JN

更新日期：1976-04-01 00:00:00

abstract：:The method of choice for the detection of Lassa virus is reverse transcription (RT)-PCR. However, the high degree of genetic variability of the virus poses a problem with the design of RT-PCR assays that will reliably detect all strains. Recently, we encountered difficulties in detecting some strains from Liberia and ...

journal_title：Journal of clinical microbiology

authors： Olschläger S,Lelke M,Emmerich P,Panning M,Drosten C,Hass M,Asogun D,Ehichioya D,Omilabu S,Günther S

更新日期：2010-06-01 00:00:00

abstract：:A quantitative modification of the shell vial assay was used to investigate cytomegalovirus viremia in solid-organ transplant recipients. The level of viremia detected in 109 of 407 specimens ranged from 0.02 to 28 infectious foci per 100,000 leukocytes. By using a Poisson model, a technique was developed to determine...

journal_title：Journal of clinical microbiology

authors： Buller RS,Bailey TC,Ettinger NA,Keener M,Langlois T,Miller JP,Storch GA

更新日期：1992-10-01 00:00:00

abstract：:Because relatively few caprine arthritis-encephalitis virus (CAEV)-infected animals exhibit clinical signs of illness, efforts to control and eradicate this virus will depend heavily on a sensitive diagnostic test that can be easily carried out. The currently utilized tests are of limited usefulness because of relativ...

journal_title：Journal of clinical microbiology

authors： Archambault D,East N,Perk K,Dahlberg JE

更新日期：1988-05-01 00:00:00

abstract：:During lyssavirus surveillance, 1,221 bats of at least 30 species were collected from 25 locations in Kenya. One isolate of Lagos bat virus (LBV) was obtained from a dead Eidolon helvum fruit bat. The virus was most similar phylogenetically to LBV isolates from Senegal (1985) and from France (imported from Togo or Egy...

journal_title：Journal of clinical microbiology

authors： Kuzmin IV,Niezgoda M,Franka R,Agwanda B,Markotter W,Beagley JC,Urazova OY,Breiman RF,Rupprecht CE

更新日期：2008-04-01 00:00:00

abstract：:Little information is available about the genetic variability of Leishmania populations and the possible correlations with ecoepidemiological features of leishmaniases. The present study was carried out in French Guiana, a country where cutaneous leishmaniases (CL) are endemic over the whole territory. The genetic pol...

journal_title：Journal of clinical microbiology

authors： Rotureau B,Ravel C,Nacher M,Couppié P,Curtet I,Dedet JP,Carme B

更新日期：2006-02-01 00:00:00

abstract：:We report a fatal case of a chromoblastomycosis-like infection caused by a novel species of Fonsecaea in a 52-year-old immunocompetent Caucasian male from an area of chromoblastomycosis endemicity in Brazil. The patient had a 30-year history of slowly evolving, verrucous lesions on the right upper arm which gradually ...

journal_title：Journal of clinical microbiology

authors： de Azevedo CM,Gomes RR,Vicente VA,Santos DW,Marques SG,do Nascimento MM,Andrade CE,Silva RR,Queiroz-Telles F,de Hoog GS

更新日期：2015-08-01 00:00:00

abstract：:We isolated and characterized a Vibrio cholerae O139 Bengal-specific DNA region by arbitrary PCR. The fragment contains open reading frames encoding two potential glycosyltransferases possibly involved in capsular polysaccharide or lipopolysaccharide biosynthesis. In order to evaluate the possibility that this region ...

journal_title：Journal of clinical microbiology

authors： Falklind S,Stark M,Albert MJ,Uhlén M,Lundeberg J,Weintraub A

更新日期：1996-12-01 00:00:00

abstract：:Among 452 samples that were positive by the Xpert MTB/RIF (Xpert) assay and MGIT 960 system (MGIT), 440 and 10 Mycobacterium tuberculosis samples were detected as rifampin susceptible and rifampin resistant, respectively. Two isolates that were rifampin susceptible by the MGIT system were rifampin resistant by the Xpe...

journal_title：Journal of clinical microbiology

authors： Mokaddas E,Ahmad S,Eldeen HS,Al-Mutairi N

更新日期：2015-04-01 00:00:00

abstract：:A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the res...

journal_title：Journal of clinical microbiology

authors： Liang SY,Chan YH,Hsia KT,Lee JL,Kuo MC,Hwa KY,Chan CW,Chiang TY,Chen JS,Wu FT,Ji DD

更新日期：2009-06-01 00:00:00

abstract：:We report a case of a 27-year-old male who presented with respiratory distress that required mechanical ventilation. Transbronchial biopsy revealed adiaspores of the fungus Emmonsia crescens within granulomata, a condition known as adiaspiromycosis. The patient received amphotericin products and corticosteroids, follo...

journal_title：Journal of clinical microbiology

authors： Anstead GM,Sutton DA,Graybill JR

更新日期：2012-04-01 00:00:00

abstract：:Lipooligosaccharide configurations were predicted in nontypeable Haemophilus influenzae isolates based on the presence of seven oligosaccharide extension-initiating genes (or alleles). Predicted configurations with 2 to 3 oligosaccharide extensions were more prevalent among middle ear than throat strains. In addition,...

journal_title：Journal of clinical microbiology

authors： McCrea KW,Xie J,Daniel D,Ulrich-Lewis JT,Zhang L

更新日期：2014-07-01 00:00:00

abstract：:Lipopolysaccharide (LPS) of a reference strain of Yersinia enterocolitica serovar O15, Ye614, was examined for its chemical structure and antigenicity. The O15 LPS was partially hydrolyzed with diluted acetic acid and fractionated on a Sephadex G-50 column. Only one peak was detected at fractions where the R-core olig...

journal_title：Journal of clinical microbiology

authors： Kawaoka Y,Wauters G,Otsuki K,Tsubokura M

更新日期：1986-08-01 00:00:00

abstract：:Lysis and filtration of blood culture specimens were combined with impedance detection of bacterial growth to facilitate the diagnosis of bacteremia. A blood lysis-filtration technique (Zierdt et al., 1976) was coupled to a simple, inexpensive automated detection system. The practical and technical aspects of the impe...

journal_title：Journal of clinical microbiology

authors： Kagan RL,Schuette WH,Zierdt CH,MacLowry JD

更新日期：1977-01-01 00:00:00

abstract：:We compared the BD MAX enteric bacterial panel (EBP) to culture for the detection of Salmonella, Shigella, Campylobacter, and Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157 in seeded stool samples. The EBP panel demonstrated superior sensitivity and reliably detected Salmonella, EHEC O157, Shigel...

journal_title：Journal of clinical microbiology

authors： Anderson NW,Buchan BW,Ledeboer NA

更新日期：2014-04-01 00:00:00

abstract：:A disease similar to ulcerative colitis in humans has been identified in cotton-top tamarins (CTTs) in captivity. The clinical signs include weight loss, diarrhea, and rectal bleeding with the pathological features and biochemical abnormalities of ulcerative colitis. Approximately 25 to 40% of these animals develop co...

journal_title：Journal of clinical microbiology

authors： Saunders KE,Shen Z,Dewhirst FE,Paster BJ,Dangler CA,Fox JG

更新日期：1999-01-01 00:00:00

abstract：:The aim of this study was to determine if gastroenteric viruses were present on surfaces and equipment in a pediatric primary immunodeficiency unit (PPIU) by environmental sampling using swabs and subsequent nucleic acid extraction and reverse transcriptase PCR assays. A PPIU was chosen, and 11 swabs were taken at the...

journal_title：Journal of clinical microbiology

authors： Gallimore CI,Taylor C,Gennery AR,Cant AJ,Galloway A,Iturriza-Gomara M,Gray JJ

更新日期：2006-02-01 00:00:00

abstract：:Two previously demonstrated sensitive and specific enzyme immunoassays (EIAs) for rotavirus, one using polyclonal and monoclonal antisera (TestPack Rotavirus [TPK]; Abbott Laboratories) and the other using only monoclonal anti-rotavirus antibodies (Rotaclone [RTC]; Cambridge BioScience Corporation), were evaluated as ...

journal_title：Journal of clinical microbiology

authors： Dennehy PH,Gauntlett DR,Spangenberger SE

更新日期：1990-06-01 00:00:00

abstract：:Automation of the clinical microbiology laboratory has become more prominent as laboratories face higher specimen volumes and understaffing and are becoming more consolidated. One recent advancement is the use of digital image analysis to rapidly distinguish between chromogenic growth for screening bacterial cultures....

journal_title：Journal of clinical microbiology

authors： Faron ML,Buchan BW,Relich RF,Clark J,Ledeboer NA

更新日期：2020-03-25 00:00:00

abstract：:Streptococcus agalactiae causes severe invasive disease in humans and mastitis in cattle. Temporally matched bovine milk isolates and clinical human invasive isolates (52 each) collected in New York State over 18 months were characterized by molecular subtyping and phenotypic methods to probe the interspecies transmis...

journal_title：Journal of clinical microbiology

authors： Sukhnanand S,Dogan B,Ayodele MO,Zadoks RN,Craver MP,Dumas NB,Schukken YH,Boor KJ,Wiedmann M

更新日期：2005-03-01 00:00:00

abstract：:Spirochetes of the genus Treponema were cultured from 7 of 10 cases of digital dermatitis in sheep. Two cultures comprised Treponema phagedenis-like and Treponema medium/Treponema vincentii-like spirochetes, respectively, while the remaining cultures comprised mixed populations of Treponema medium/Treponema vincentii-...

journal_title：Journal of clinical microbiology

authors： Sayers G,Marques PX,Evans NJ,O'Grady L,Doherty ML,Carter SD,Nally JE

更新日期：2009-04-01 00:00:00

abstract：:Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral zoonosis which occurs throughout Africa, Eastern Europe, and Asia and results in an approximately 30% fatality rate. A reverse transcription-PCR assay including a competitive internal control was developed on the basis of the most up-to-date genome informati...

journal_title：Journal of clinical microbiology

authors： Wölfel R,Paweska JT,Petersen N,Grobbelaar AA,Leman PA,Hewson R,Georges-Courbot MC,Papa A,Heiser V,Panning M,Günther S,Drosten C

更新日期：2009-04-01 00:00:00

abstract：:A microwave-accelerated direct immunofluorescence staining method which requires only 20 min from specimen receipt to interpretation is as effective as conventional methods for detecting respiratory syncytial virus and influenza A virus antigens in clinical specimens. The time required compares favorably with that for...

journal_title：Journal of clinical microbiology

authors： Hite SA,Huang YT

更新日期：1996-07-01 00:00:00

abstract：:We mapped the coding single nucleotide polymorphisms in four toxin genes-exoS, exoT, exoU, and exoY-of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes....

journal_title：Journal of clinical microbiology

authors： Ajayi T,Allmond LR,Sawa T,Wiener-Kronish JP

更新日期：2003-08-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) based on the detection of specific antibodies in serum are commonly used for the diagnosis of visceral leishmaniasis (VL). Several commercial kits are available, and some of them allow the use of whole-blood samples instead of serum. An RDT is much more user-friendly for blood samples tha...

journal_title：Journal of clinical microbiology

authors： Kumar D,Khanal B,Tiwary P,Mudavath SL,Tiwary NK,Singh R,Koirala K,Boelaert M,Rijal S,Sundar S

更新日期：2013-12-01 00:00:00

abstract：:We analyzed the characteristics of subjects from the same area who were infected with hepatitis C virus genotypes 1 through 4 and subtypes 1a and 1b. Our data are consistent with a rapid evolution in the epidemiology of HCV genotypes and argue against different pathogenic potentials for genotypes 1b and 2. ...

journal_title：Journal of clinical microbiology

authors： Ravaggi A,Rossini A,Mazza C,Puoti M,Marin MG,Cariani E

更新日期：1996-11-01 00:00:00

abstract：:Blood culture records from 1994 to 1995 from five U.S. medical centers all using the Difco ESP continuous monitoring blood culture system were reviewed retrospectively. Among a total of 7,362 isolates of bacteria and yeasts, only 0.1% of possibly significant isolates would have been missed had blood cultures been rout...

journal_title：Journal of clinical microbiology

authors： Doern GV,Brueggemann AB,Dunne WM,Jenkins SG,Halstead DC,McLaughlin JC

更新日期：1997-05-01 00:00:00

abstract：:Subgroup J avian leukosis virus (ALV-J) is an avian retrovirus that causes severe economic losses in the poultry industry. The early identification and removal of virus-shedding birds are important to reduce the spread of congenital and contact infections. In this study, a TaqMan-based real-time PCR method for the rap...

journal_title：Journal of clinical microbiology

authors： Qin L,Gao Y,Ni W,Sun M,Wang Y,Yin C,Qi X,Gao H,Wang X

更新日期：2013-01-01 00:00:00