Environmental monitoring for gastroenteric viruses in a pediatric primary immunodeficiency unit.

abstract：:A novel reverse primer (GLM MR1) was designed for detection of the glmM gene in Helicobacter pylori by PCR. The percentage of amplification in clinical isolates using GLM MR1 was 100% for detection of the glmM gene and 86.36% for the ureA gene. The primer designed is useful for the identification of H. pylori. ...

journal_title：Journal of clinical microbiology

authors： Espinoza MG,Vazquez RG,Mendez IM,Vargas CR,Cerezo SG

更新日期：2011-04-01 00:00:00

abstract：:Molecular analysis of Mycobacterium ulcerans has revealed two new insertion sequences (ISs), IS2404 and IS2606. IS2404 was identified by complete sequencing of a previously described repetitive DNA segment from M. ulcerans. This element is 1,274 bp long, contains 12-bp inverted repeats and a single open reading frame ...

journal_title：Journal of clinical microbiology

authors： Stinear T,Ross BC,Davies JK,Marino L,Robins-Browne RM,Oppedisano F,Sievers A,Johnson PD

更新日期：1999-04-01 00:00:00

abstract：:A cross-reaction between Acinetobacter calcoaceticus and chlamydiae is described. A water-soluble, heat stable, non-dialyzable antigen was extracted from Acinetobacter species by boiling. This antigen fixed complement in the presence of homologous hyperimmune sera from rabbits or guinea pigs and in the presence of het...

journal_title：Journal of clinical microbiology

authors： Brade H,Brunner H

更新日期：1979-12-01 00:00:00

abstract：:Serological tests to detect Trypanosoma cruzi antibodies have been used for screening blood donors, for epidemic studies, and for diagnosis of probably infected persons. Among different tests, the enzyme-linked immunosorbent assay (ELISA) with total, semipurified, or synthetic antigens has been widely used, mainly due...

journal_title：Journal of clinical microbiology

authors： Ferreira AW,Belem ZR,Lemos EA,Reed SG,Campos-Neto A

更新日期：2001-12-01 00:00:00

abstract：:The risk factors for relapse of methicillin-resistant Staphylococcus aureus (MRSA) bacteremia after vancomycin treatment are unknown. Diversilab typing was used to classify recurrent bacteremia as relapse or reinfection. Bacteremia for >7 days and staphylococcal cassette chromosome mec element (SCCmec) type II were in...

journal_title：Journal of clinical microbiology

authors： Welsh KJ,Skrobarcek KA,Abbott AN,Lewis CT,Kruzel MC,Lewis EM,Gardiner JM,Mohr JF,Armitige LY,Wanger A

更新日期：2011-10-01 00:00:00

abstract：:Coronavirus disease (COVID) serological tests are essential to determine the overall seroprevalence of a population and to facilitate exposure estimates within that population. We performed a head-to-head assessment of enzyme immunoassays (EIAs) and point-of-care lateral flow assays (POCTs) to detect severe acute resp...

journal_title：Journal of clinical microbiology

authors： Charlton CL,Kanji JN,Johal K,Bailey A,Plitt SS,MacDonald C,Kunst A,Buss E,Burnes LE,Fonseca K,Berenger BM,Schnabl K,Hu J,Stokes W,Zelyas N,Tipples G

更新日期：2020-09-22 00:00:00

abstract：:Widespread use of conjugate pneumococcal polysaccharide-protein vaccines may alter the spectrum of pneumococci producing invasive disease. Novel sensitive diagnostic methods would be valuable for monitoring the epidemiology of pneumococcal disease within populations and vaccine recipients. Ideally, these methods shoul...

journal_title：Journal of clinical microbiology

authors： Leeming JP,Cartwright K,Morris R,Martin SA,Smith MD,South-West Pneumococcus Study Group.

更新日期：2005-10-01 00:00:00

abstract：:A total of 1,835 Yersinia spp. were isolated from 925 (60.5%) of 1,530 wild mice and from 139 (79.9%) of 174 moles living in mountainous areas of eastern Shimane Prefecture, Japan. The Yersinia spp. included 1,106 Yersinia enterocolitica, 26 Y. enterocolitica-like, 176 Yersinia mollaretii, 149 Yersinia frederiksenii, ...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Gomyoda M,Kaneko S

更新日期：1990-11-01 00:00:00

abstract：:PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus ...

journal_title：Journal of clinical microbiology

authors： Spilker T,Baldwin A,Bumford A,Dowson CG,Mahenthiralingam E,LiPuma JJ

更新日期：2009-08-01 00:00:00

abstract：:The findings from a preliminary assessment of a new instrument designed for the inoculation and spreading of specimens for microbiological analysis onto agar plates are described. The study found that the instrument was able to select full or biplates from a number of input cassettes, each containing different agar ty...

journal_title：Journal of clinical microbiology

authors： Glasson JH,Guthrie LH,Nielsen DJ,Bethell FA

更新日期：2008-04-01 00:00:00

abstract：:We describe the serum immunoglobulin A (IgA) antibody response to Norwalk virus infection in human volunteers and compare it with previously described IgM and total antibody responses. Whereas specific IgA and IgM peak within 2 weeks after onset of symptoms, titers of total blocking antibody continue to rise, implying...

journal_title：Journal of clinical microbiology

authors： Erdman DD,Gary GW,Anderson LJ

更新日期：1989-06-01 00:00:00

abstract：:Two multiplex PCR enzyme immunoassays (PCR-EIAs) were developed for Staphylococcus aureus exotoxin gene screening as an alternative to the conventional biological assays, which depend on detectable amounts of toxins produced. One set of oligonucleotide primers and probes was designed to search for enterotoxin A to E g...

journal_title：Journal of clinical microbiology

authors： Becker K,Roth R,Peters G

更新日期：1998-09-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the titration of antibodies in human sera to influenza virus neuraminidase, employing partially purified N1 neuraminidase. Specificity of the test was demonstrated, and the test was more sensitive than either the conventional neuraminidase inhibition or plaque siz...

journal_title：Journal of clinical microbiology

authors： Khan MW,Gallagher M,Bucher D,Cerini CP,Kilbourne ED

更新日期：1982-07-01 00:00:00

abstract：:Total immunoglobulin E (IgE) and specific IgE were assayed by a radioimmunosorbent test and a reverse enzyme immunoassay in patients with hookworm infections before and after treatment. A total of 77 subjects (30 patients with hookworm infections and 47 subjects as controls) were studied. Both specific and total IgE l...

journal_title：Journal of clinical microbiology

authors： Ganguly NK,Mahajan RC,Sehgal R,Shetty P,Dilawari JB

更新日期：1988-04-01 00:00:00

abstract：:Confirmative diagnosis of visceral leishmaniasis (VL) is still a challenge at the primary health care facilities in most of the rural areas of endemicity in the Indian subcontinent. Conventional methods for parasitological confirmation are risky and require skilled personnel, and hence they are unavailable to the poor...

journal_title：Journal of clinical microbiology

authors： Salam MA,Khan MG,Bhaskar KR,Afrad MH,Huda MM,Mondal D

更新日期：2012-03-01 00:00:00

abstract：:Patients with infection from bacteremic Bartonella spp., tested using Bartonella Alphaproteobacteria growth medium (BAPGM), were retrospectively categorized into one of two groups that included those whose blood was collected once (group 1; n = 55) or three times (group 2; n = 36) within a 1-week period. Overall, 19 p...

journal_title：Journal of clinical microbiology

authors： Pultorak EL,Maggi RG,Mascarelli PE,Breitschwerdt EB

更新日期：2013-06-01 00:00:00

abstract：:Early and accurate diagnosis is essential for optimal therapeutic outcomes in patients infected with HIV. Currently, none of the commercially available fourth-generation assays differentiate HIV-1 and HIV-2 antibodies (Ab) or the HIV-1 p24 antigen (Ag). The aim of this study was to evaluate the performance of a novel ...

journal_title：Journal of clinical microbiology

authors： Salmona M,Delarue S,Delaugerre C,Simon F,Maylin S

更新日期：2014-01-01 00:00:00

abstract：:Fourth-generation human immunodeficiency virus (HIV) screening immunoassays reduce the diagnostic window between infection and diagnosis by the inclusion of HIV p24 antigen detection together with HIV antibody detection in the same test. We compared third- and fourth-generation HIV immunoassays and a dedicated HIV p24...

journal_title：Journal of clinical microbiology

authors： Speers D,Phillips P,Dyer J

更新日期：2005-10-01 00:00:00

abstract：:Several patients receiving blood transfusions during the summer of 1991 developed bacteremia after the transfusion. In all cases, the infection was caused by Serratia marcescens. The same strain of Serratia marcescens was isolated from the patients and from the outer surface of unfilled blood bags. The transport conta...

journal_title：Journal of clinical microbiology

authors： Szewzyk U,Szewzyk R,Stenström TA

更新日期：1993-07-01 00:00:00

abstract：:Brucellosis is a globally significant zoonosis, the control of which is difficult and resource intensive. Serological tests form a vital part of a multifactorial approach to control and are often performed in large numbers. The aim of the present study was to develop a new assay to improve the efficiency, ease, and ef...

journal_title：Journal of clinical microbiology

authors： McGiven JA,Thompson IJ,Commander NJ,Stack JA

更新日期：2009-10-01 00:00:00

abstract：:A slight modification of the chemically defined medium of McVeigh and Morton resulted in an excellent substratum for the cultivation of Paracoccidioides brasiliensis yeast phase. ...

journal_title：Journal of clinical microbiology

authors： Restrepo A,Jiménez BE

更新日期：1980-08-01 00:00:00

abstract：:A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reasses...

journal_title：Journal of clinical microbiology

authors： Kondas AV,Olson VA,Li Y,Abel J,Laker M,Rose L,Wilkins K,Turner J,Kline R,Damon IK

更新日期：2015-04-01 00:00:00

abstract：:The Alere i Influenza A&B assay is a newly developed rapid molecular assay which has the potential to generate results within 15 min from sample collection. In this study, we evaluated the Alere i Influenza A&B assay by using salvaged frozen respiratory specimens that were collected in viral transport medium from chil...

journal_title：Journal of clinical microbiology

authors： Bell JJ,Selvarangan R

更新日期：2014-11-01 00:00:00

abstract：:The Oxi-ferm tube, an eight-compartment system suitable for the recognition of nonfermentative, gram-negative, rod-shaped bacteria, was evaluated in conjunction with the oxidase test in a clinical microbiology laboratory setting. A comparison between this system and the 17-test routine examination was performed with 2...

journal_title：Journal of clinical microbiology

authors： Isenberg HD,Sampson-Scherer J

更新日期：1977-03-01 00:00:00

abstract：:A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was...

journal_title：Journal of clinical microbiology

authors： Rench MA,Metzger TG,Baker CJ

更新日期：1984-11-01 00:00:00

abstract：:Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the...

journal_title：Journal of clinical microbiology

authors： Carlson JR,McCarthy LR

更新日期：1979-03-01 00:00:00

abstract：:A novel PCR primer pair was used to detect the presence of cmeC in 131 Campylobacter jejuni and C. coli strains isolated from various hosts (cattle, turkeys, humans, and pigs). DNA sequence analysis revealed a high degree of genetic variation between the two species, while extremely limited genetic variation among iso...

journal_title：Journal of clinical microbiology

authors： Fakhr MK,Logue CM

更新日期：2007-10-01 00:00:00

abstract：:A total of 201 Mycoplasma pneumoniae clinical isolates from Beijing, China, isolated from 2008 to 2011, were clustered into 16 multiple-locus variable-number tandem-repeat analysis (MLVA) types, of which 6 new MLVA types have never been reported previously. Type 1 isolates based on p1 gene genotyping were mainly MLVA ...

journal_title：Journal of clinical microbiology

authors： Zhao F,Liu G,Cao B,Wu J,Gu Y,He L,Meng F,Zhu L,Yin Y,Lv M,Zhang J

更新日期：2013-02-01 00:00:00

abstract：:Serum antibody responses to influenza A virus infection were examined in 388 normal subjects during a trial of chemoprophylaxis in an outbreak of influenza A in 1980-1981 in which both A/H1N1 and A/H3N2 viruses circulated. Paired serum specimens obtained over a 6-week period were tested for antibodies to both A/H1N1 a...

journal_title：Journal of clinical microbiology

authors： Madore HP,Reichman RC,Dolin R

更新日期：1983-12-01 00:00:00

abstract：:The COVID-19 pandemic has necessitated a multifaceted rapid response by the scientific community, bringing researchers, health officials, and industry together to address the ongoing public health emergency. To meet this challenge, participants need an informed approach for working safely with the etiological agent, t...

journal_title：Journal of clinical microbiology

authors： Welch SR,Davies KA,Buczkowski H,Hettiarachchi N,Green N,Arnold U,Jones M,Hannah MJ,Evans R,Burton C,Burton JE,Guiver M,Cane PA,Woodford N,Bruce CB,Roberts ADG,Killip MJ

更新日期：2020-10-21 00:00:00