Combination assay detecting both human immunodeficiency virus (HIV) p24 antigen and anti-HIV antibodies opens a second diagnostic window.

abstract：:The aim of this study was to describe the frequency of positive Aspergillus tests in COVID-19 patients and investigate the association between COVID-19 and a positive Aspergillus test result. We compared the proportion of positive Aspergillus tests in COVID-19 patients admitted to the ICU for > 24 hours with two contr...

journal_title：Journal of clinical microbiology

authors： Yusuf E,Vonk A,van den Akker JPC,Bode L,Sips GJ,Rijnders BJA,de Steenwinkel J,Verkaik NJ,Vogel M,van der Eerden M,van Westreenen M

更新日期：2020-12-04 00:00:00

abstract：:A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Maher LA,Howell AW

更新日期：1990-03-01 00:00:00

abstract：:Neisseria meningitidis is the causative agent of meningococcal sepsis and meningitis. Neisseria polysaccharea is a nonpathogenic species. N. polysaccharea is able to use sucrose to produce amylopectin, a starch-like polysaccharide, which distinguishes it biochemically from the pathogenic species N. meningitidis. The d...

journal_title：Journal of clinical microbiology

authors： Zhu P,Tsang RS,Tsai CM

更新日期：2003-01-01 00:00:00

abstract：:Virology laboratories historically have used direct fluorescent-antibody assay (DFA) and culture to detect six or seven respiratory viruses. Following the discovery of five new human respiratory viruses since 2000, there is an increasing need for diagnostic tests to detect these emerging viruses. We have developed a n...

journal_title：Journal of clinical microbiology

authors： Mahony J,Chong S,Merante F,Yaghoubian S,Sinha T,Lisle C,Janeczko R

更新日期：2007-09-01 00:00:00

abstract：:This study estimated the regional and age- and gender-specific seroprevalences of herpes simplex virus type 1 (HSV-1) and HSV-2 in Ontario, Canada. Stored serum specimens from subjects aged 15 to 44 years, including men (n = 979), women not under prenatal care (n = 638), and women under prenatal care (n = 701) submitt...

journal_title：Journal of clinical microbiology

authors： Howard M,Sellors JW,Jang D,Robinson NJ,Fearon M,Kaczorowski J,Chernesky M

更新日期：2003-01-01 00:00:00

abstract：:Despite the declining rate of sexually transmitted diseases in developed countries, trichomoniasis is still one of the most common venereal infections. While diagnosis of this condition is commonly based on the microscopic wet-mount method, culture remains the most accurate single procedure for detecting the presence ...

journal_title：Journal of clinical microbiology

authors： Poch F,Levin D,Levin S,Dan M

更新日期：1996-10-01 00:00:00

abstract：:The Aspergillus galactomannan test was performed on cerebrospinal fluid and serum samples from 5 patients with probable cerebral aspergillosis and from 16 control patients. Cerebrospinal fluid galactomannan levels were significantly higher in aspergillosis patients, and most galactomannan was produced intrathecally. C...

journal_title：Journal of clinical microbiology

authors： Viscoli C,Machetti M,Gazzola P,De Maria A,Paola D,Van Lint MT,Gualandi F,Truini M,Bacigalupo A

更新日期：2002-04-01 00:00:00

abstract：:In order to determine whether mixed infections of human herpesvirus 6B (HHV-6B) occur in immunocompetent and immunocompromised individuals, we examined the copy numbers of telomeric repeat sequences (TRS) of clinical isolates. In clinical isolates obtained from patients with exanthem subitum caused by primary HHV-6B i...

journal_title：Journal of clinical microbiology

authors： Kato Y,Ihira M,Umeda M,Higashimoto Y,Kawamura Y,Ohashi M,Ishi J,Yoshikawa T

更新日期：2014-02-01 00:00:00

abstract：:Recent Lassa virus strains from Nigeria were completely or partially sequenced. Phylogenetic analysis revealed the predominance of lineage II and III strains, the existence of a previously undescribed (sub)lineage in Nigeria, and the directional spread of virus in the southern part of the country. The Bayesian analysi...

journal_title：Journal of clinical microbiology

authors： Ehichioya DU,Hass M,Becker-Ziaja B,Ehimuan J,Asogun DA,Fichet-Calvet E,Kleinsteuber K,Lelke M,ter Meulen J,Akpede GO,Omilabu SA,Günther S,Olschläger S

更新日期：2011-03-01 00:00:00

abstract：:The amino acids L-arginine, L-isoleucine, L-leucine, L-methionine, L-serine, L-threonine, and L-valine were essential for the growth of Legionella pneumophila in a chemically defined medium. A partial requirement for L-cysteine (or L-cystine) was also observed. A minimal medium containing only the eight required amino...

journal_title：Journal of clinical microbiology

authors： Tesh MJ,Miller RD

更新日期：1981-05-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection is an increasing health problem worldwide. Quantitative assays for HCV viral load are valuable in predicting response to therapy and for following treatment efficacy. Unfortunately, most quantitative tests for HCV RNA are limited by poor sensitivity. We have developed a convenient, hi...

journal_title：Journal of clinical microbiology

authors： Cook L,Ng KW,Bagabag A,Corey L,Jerome KR

更新日期：2004-09-01 00:00:00

abstract：:Diarrheal diseases are highly prevalent in Bangladesh. However, the relative contribution of diarrheagenic Escherichia coli organisms--those that are enterotoxigenic (ETEC), enteropathogenic (EPEC), enteroinvasive, enterohemorrhagic, enteroaggregative, and diffuse adherent--to diarrhea in Bangladeshi populations is no...

journal_title：Journal of clinical microbiology

authors： Albert MJ,Faruque SM,Faruque AS,Neogi PK,Ansaruzzaman M,Bhuiyan NA,Alam K,Akbar MS

更新日期：1995-04-01 00:00:00

abstract：:Of a total of 1043 macrolide-resistant Streptococcus pneumoniae isolates collected from 24 countries as part of PROTEKT 1999-2000, 71 isolates tested positive for both the mef(A) and erm(B) genes. Of 69 isolates subjected to further molecular investigations, all were resistant to tetracycline, 63 (91.3%) were resistan...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Morrissey I,Bakker S,Morris L,Buckridge S,Felmingham D

更新日期：2004-02-01 00:00:00

abstract：:Genetic diversity among Borrelia burgdorferi isolates recovered from the skin of Lyme disease patients was assessed by ribosomal DNA (rDNA) spacer restriction fragment length polymorphism analysis, genomic restriction site polymorphism analysis, and plasmid content analysis. There was a significant association between...

journal_title：Journal of clinical microbiology

authors： Iyer R,Liveris D,Adams A,Nowakowski J,McKenna D,Bittker S,Cooper D,Wormser GP,Schwartz I

更新日期：2001-08-01 00:00:00

abstract：:Rapid and reliable detection of methicillin-resistant Staphylococcus aureus (MRSA) carriers is crucial for the effective control of MRSA transmission in healthcare facilities. The aim of this study was to verify the performance of the IDI-MRSA real-time PCR assay for direct MRSA detection in diverse mucocutaneous swab...

journal_title：Journal of clinical microbiology

authors： de San N,Denis O,Gasasira MF,De Mendonça R,Nonhoff C,Struelens MJ

更新日期：2007-04-01 00:00:00

abstract：:A comparison of three different commercial media was made to assess their recovery of anaerobic organisms from the blood stream. The three media used were the 50-ml brain heart infusion broth with added CO2 (Pfizer), the 50-ml Thiol broth with added CO2 (Difco), and the 50-ml prereduced, supplemented peptone broth in ...

journal_title：Journal of clinical microbiology

authors： Mangels JI,Lindberg LH,Vosti KL

更新日期：1977-05-01 00:00:00

abstract：:In this report we present clinical descriptions of 12 Hawaiian patients from whom Escherichia vulneris or E. hermannii strains were isolated. All but two patients had soft-tissue infections with multiple bacteria, particularly Staphylococcus aureus. The other two had purulent conjunctivitis associated with S. aureus a...

journal_title：Journal of clinical microbiology

authors： Pien FD,Shrum S,Swenson JM,Hill BC,Thornsberry C,Farmer JJ 3rd

更新日期：1985-08-01 00:00:00

abstract：:Whole-genome sequencing (WGS) is a highly sensitive method for identifying genetic relatedness and transmission of Clostridioides difficile strains. Previous studies suggest that as few as 3 core genome single-nucleotide variants (SNVs) discriminate between genetically distinct isolates. Because a single C. difficile ...

journal_title：Journal of clinical microbiology

authors： Balaji A,Ozer EA,Kociolek LK

更新日期：2019-08-26 00:00:00

abstract：:Diagnostic tests for respiratory viral infections have traditionally been performed on nasopharyngeal swabs or washings. Reverse transcriptase PCR (RT-PCR) is rapid, sensitive, and specific for viral infection diagnosis but is rarely applied to sputum samples. Thus, we evaluated the diagnostic yield of RT-PCR for dete...

journal_title：Journal of clinical microbiology

authors： Falsey AR,Formica MA,Walsh EE

更新日期：2012-01-01 00:00:00

abstract：:A quantitative PCR assay for the detection of human herpesvirus 6 (HHV-6) (variants A and B) and HHV-7 DNAs in clinical samples was developed. The assay uses a nonhomologous internal standard (IS) for each virus that is coamplified with the wild-type target sequence in the same vial and with the same pair of primers. ...

journal_title：Journal of clinical microbiology

authors： Secchiero P,Zella D,Crowley RW,Gallo RC,Lusso P

更新日期：1995-08-01 00:00:00

abstract：:Haemophilus ducreyi ATCC 33922, a virulent, well-characterized strain, was used to construct a genomic library in a bacteriophage expression vector. Three DNA fragments were selected for use as probes on the basis of their ability to encode H. ducreyi-specific proteins, as demonstrated by reactivity with rabbit polycl...

journal_title：Journal of clinical microbiology

authors： Parsons LM,Shayegani M,Waring AL,Bopp LH

更新日期：1989-07-01 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of an ongoing pandemic that has infected over 36 million and killed over 1 million people. Informed implementation of government public health policies depends on accurate data on SARS-CoV-2 immunity at a population scale. We hypothesized that d...

journal_title：Journal of clinical microbiology

authors： Pisanic N,Randad PR,Kruczynski K,Manabe YC,Thomas DL,Pekosz A,Klein SL,Betenbaugh MJ,Clarke WA,Laeyendecker O,Caturegli PP,Larman HB,Detrick B,Fairley JK,Sherman AC,Rouphael N,Edupuganti S,Granger DA,Granger SW,Coll

更新日期：2020-12-17 00:00:00

abstract：:Of 35 stool specimens isolated and examined in 293 cells, 15 isolates contained adenovirus species 40 (Ad40), and 4 of these 15 isolates also contained a nonfastidious adenovirus species (Ad1 in two cases, Ad18 or Ad31) which was selected over Ad40 during serial passage in the 293 cells. The selection of Ad1 over Ad40...

journal_title：Journal of clinical microbiology

authors： Brown M

更新日期：1985-08-01 00:00:00

abstract：:Time to detection (TTD) in automated blood culture systems is delayed for sensitive microorganisms in the presence of antimicrobial substances and has been associated with worse outcomes for sepsis patients on inadequate empirical therapy. While resin addition removes antimicrobial substances to various degrees from b...

journal_title：Journal of clinical microbiology

authors： Mitteregger D,Barousch W,Nehr M,Kundi M,Zeitlinger M,Makristathis A,Hirschl AM

更新日期：2013-05-01 00:00:00

abstract：:Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. T...

journal_title：Journal of clinical microbiology

authors： Giacomelli LR,Helbel C,Ogassawara RL,Barreto AM,Martins FM,Cardoso CL,Leite CQ

更新日期：2005-08-01 00:00:00

abstract：:The performance of a direct sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was compared with that of the Pastorex Aspergillus antigen latex agglutination (LA) test by using 532 serum samples from 61 patients at risk for invasive aspergillosis. The ELISA gave positive results...

journal_title：Journal of clinical microbiology

authors： Verweij PE,Stynen D,Rijs AJ,de Pauw BE,Hoogkamp-Korstanje JA,Meis JF

更新日期：1995-07-01 00:00:00

abstract：:The correlation between response to antiviral therapy and pretreatment viral load in patients with chronic hepatitis C has prompted the development of quantitative assays to measure viral load. The aim of our study was to assess the clinical relevance of the newly developed semiautomated PCR system COBAS HCV MONITOR v...

journal_title：Journal of clinical microbiology

authors： Martinot-Peignoux M,Le Breton V,Fritsch S,Le Guludec G,Labouret N,Keller F,Marcellin P

更新日期：2000-07-01 00:00:00

abstract：:In 1998, 21 inhabitants of a German nursing home fell ill with acute gastroenteritis after consumption of minced beef heart (P. Graf and L. Bader, Epidemiol. Bull. 41:327-329, 2000). Two residents died during hospital treatment. Seventeen Clostridium perfringens strains were collected from two different dishes and fro...

journal_title：Journal of clinical microbiology

authors： Schalch B,Bader L,Schau HP,Bergmann R,Rometsch A,Maydl G,Kessler S

更新日期：2003-02-01 00:00:00

abstract：:Pyrazinamide is important in tuberculosis treatment, as it is bactericidal to semidormant mycobacteria not killed by other antituberculosis drugs. Pyrazinamide is also one of the cornerstone drugs retained in the treatment of multidrug-resistant tuberculosis (MDR-TB). However, due to technical difficulties, routine dr...

journal_title：Journal of clinical microbiology

authors： Mphahlele M,Syre H,Valvatne H,Stavrum R,Mannsåker T,Muthivhi T,Weyer K,Fourie PB,Grewal HM

更新日期：2008-10-01 00:00:00

abstract：:We have used comparative genomic hybridization (CGH) on a full-genome Campylobacter jejuni microarray to examine genome-wide gene conservation patterns among 51 strains isolated from food and clinical sources. These data have been integrated with data from three previous C. jejuni CGH studies to perform a meta-analysi...

journal_title：Journal of clinical microbiology

authors： Taboada EN,Acedillo RR,Carrillo CD,Findlay WA,Medeiros DT,Mykytczuk OL,Roberts MJ,Valencia CA,Farber JM,Nash JH

更新日期：2004-10-01 00:00:00