Selection of nonfastidious adenovirus species in 293 cells inoculated with stool specimens containing adenovirus 40.

abstract：:In this report, we present a case of rhino-orbital mucormycosis in a 57-year-old female with poorly controlled diabetes mellitus. The only mold cultured at 25°C, 37°C, and 40°C from a specimen of the nasal crust was identified phenotypically and independently using nuclear ribosomal DNA sequence data as Thamnostylum l...

journal_title：Journal of clinical microbiology

authors： Xess I,Mohapatra S,Shivaprakash MR,Chakrabarti A,Benny GL,O'Donnell K,Padhye AA

更新日期：2012-04-01 00:00:00

abstract：:A proficiency review of antituberculous drug susceptibility testing (DST) was undertaken by the regional tuberculosis reference laboratories of the Western Pacific Region of WHO to evaluate the performance of national reference laboratories (NRLs) and to ensure that the results from the participating laboratories are ...

journal_title：Journal of clinical microbiology

authors： Bai GH,Kim SJ,Chang CL,National and Regional Tuberculosis Reference Laboratories.

更新日期：2007-11-01 00:00:00

abstract：:In a cohort of subjects from Italy, anti-hepatitis C virus (HCV) and HCV RNA [HCV(+) subgroup] prevalences were 24.6 and 79.6%, respectively. HCV types 1b and 2a/c accounted for 95% of infections. Adjusted alanine aminotransferase levels were higher in males than in females and in RNA-positive subjects than in RNA-neg...

journal_title：Journal of clinical microbiology

authors： Osella AR,Sonzogni L,Cavallini A,Foti L,Guerra V,Di Leo A,Mondelli MU,Misciagna G,Silini EM

更新日期：1999-07-01 00:00:00

abstract：:Eleven isolates of Aeromonas species from human stool cultures were found to produce enterotoxin activity as determined by assay of culture filtrates in rabbit intestinal loops and rabbit skin and on adrenal Y1 cells. Hemolysin(s) and a cytotoxic protein were found to interfere in all three assay systems but could be ...

journal_title：Journal of clinical microbiology

authors： Ljungh A,Popoff M,Wadstrom T

更新日期：1977-08-01 00:00:00

abstract：:Enterocytozoon bieneusi, a microsporidian parasite, has been recognized since 1985 as an agent of intestinal microsporidiosis leading to malabsorption syndrome, diarrhea, and weight loss in AIDS patients. Recently, however, we have identified E. bieneusi spores in the sputum, bronchoalveolar lavage, and stool samples ...

journal_title：Journal of clinical microbiology

authors： del Aguila C,Lopez-Velez R,Fenoy S,Turrientes C,Cobo J,Navajas R,Visvesvara GS,Croppo GP,Da Silva AJ,Pieniazek NJ

更新日期：1997-07-01 00:00:00

abstract：:Viridans streptococci (VS) are common etiologic agents of subacute infective endocarditis and are capable of causing a variety of pyogenic infections. Many species of VS are difficult to differentiate by phenotypic traits. An oligonucleotide array based on 16S-23S rRNA gene intergenic spacer (ITS) sequences was develo...

journal_title：Journal of clinical microbiology

authors： Chen CC,Teng LJ,Kaiung S,Chang TC

更新日期：2005-04-01 00:00:00

abstract：:Various fixatives and treatments such as acetone, methanol, Bouin fixative, modified Bouin fixative, 10% Formalin, modified methacarn, periodate-lysine-paraformaldehyde, acetone-methyl benzoate-xylene, and EDTA were evaluated for their effect on the immunoreactivity of Coxiella burnetii in paraffin-embedded tissues by...

journal_title：Journal of clinical microbiology

authors： Baumgärtner W,Dettinger H,Schmeer N,Hoffmeister E

更新日期：1988-10-01 00:00:00

abstract：:An enzyme immunoassay (enzyme-linked immunosorbent assay [ELISA]) to detect hamster antibody to toxins A and B of Clostridium difficile was developed in which toxin preparations are used to coat the solid phase. The specificity of the assay was supported by blocking tests with the toxin preparations and proteins from ...

journal_title：Journal of clinical microbiology

authors： Viscidi RP,Yolken RH,Laughon BE,Bartlett JG

更新日期：1983-08-01 00:00:00

abstract：:Serum hepatitis B virus (HBV) DNA was extracted from a chronically infected patient with cocirculation of hepatitis B surface antigen (HBsAg) and anti-HBs antibodies. Direct PCR and clone-derived sequences of the S and overlapped P genes were obtained. DNA sequences and phylogenetic analysis ascribed this isolate to g...

journal_title：Journal of clinical microbiology

authors： Cuestas ML,Mathet VL,Ruiz V,Minassian ML,Rivero C,Sala A,Corach D,Alessio A,Pozzati M,Frider B,Oubiña JR

更新日期：2006-06-01 00:00:00

abstract：:During a 3-year period, all Pasteurella strains recovered at the Clinical Microbiological Laboratory, Lund, Sweden, were studied biochemically with respect to their relationship to the recently described taxa of this genus. Of 159 strains recovered from 146 infected humans, 95 were identified as Pasteurella multocida ...

journal_title：Journal of clinical microbiology

authors： Holst E,Rollof J,Larsson L,Nielsen JP

更新日期：1992-11-01 00:00:00

abstract：:Optimal conditions for a fluorescence immunoassay of antibodies to, and surface antigens of, Actinomyces viscosus ATCC 19246 are described. In the standard fluorescence immunoassay, 10(8) colony-forming units of A. viscosus reacted with an antibody preparation, were washed, and then were treated with an excess of fluo...

journal_title：Journal of clinical microbiology

authors： Gillis TP,Thompson JJ

更新日期：1978-02-01 00:00:00

abstract：:We evaluated the use of mannitol salt agar with oxacillin for use as a primary screening medium for the simultaneous detection and identification of methicillin-resistant Staphylococcus aureus in clinical surveillance specimens. Oxacillin agar dilution susceptibility tests with mannitol salt agar and Mueller-Hinton ag...

journal_title：Journal of clinical microbiology

authors： Lally RT,Ederer MN,Woolfrey BF

更新日期：1985-10-01 00:00:00

abstract：:We tested 121 pneumococcal serogroup 6 isolates (including 30 serotype 6C and 24 serotype 6D isolates) by serotype-specific PCR and the Quellung reaction, using "old" and "new" pool B, factor 6b, and new factor 6d antisera. In combination with group B and other factor antisera, factor 6d antiserum can reliably identif...

journal_title：Journal of clinical microbiology

authors： Oftadeh S,Satzke C,Gilbert GL

更新日期：2010-09-01 00:00:00

abstract：:Group A streptococcus (GAS) species cause bacterial pharyngitis in both adults and children. Early and accurate diagnosis of GAS is important for appropriate antibiotic therapy to prevent GAS sequalae. The Revogene Strep A molecular assay (Meridian Bioscience Canada Inc, Quebec City, QC, Canada) is an automated real-t...

journal_title：Journal of clinical microbiology

authors： Banerjee D,Michael J,Schmitt B,Salimnia H,Mhaissen N,Goldfarb DM,Lachance P,Faron ML,Aufderheide T,Ledeboer N,Weissfeld A,Selvarangan R

更新日期：2020-06-24 00:00:00

abstract：:Nocardia brasiliensis, the second most frequently isolated aerobic actinomycete in the clinical laboratory, is usually associated with localized cutaneous infections. However, 22% of 238 N. brasiliensis isolates from the United States and 12% of 66 isolates from Queensland, Australia, which had been collected over a 1...

journal_title：Journal of clinical microbiology

authors： Wallace RJ Jr,Brown BA,Blacklock Z,Ulrich R,Jost K,Brown JM,McNeil MM,Onyi G,Steingrube VA,Gibson J

更新日期：1995-06-01 00:00:00

abstract：:The frequency with which clue cells could be detected in Gram-stained vaginal smears and/or cervical Papanicolaou (Pap) smears was compared with the frequency of Corynebacterium vaginale (Haemophilus vaginalis) isolation in a group of 236 female patients, of whom 221 had vaginitis. Vaginal clue cells were found most o...

journal_title：Journal of clinical microbiology

authors： Smith RF,Rodgers HA,Hines PA,Ray RM

更新日期：1977-03-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:Accreditation in Ontario, Canada, requires that licensed clinical laboratories participate in external quality assessment (also known as proficiency testing) and perform competency evaluation of their staff. To assess the extent of ongoing competency assessment practices, the Quality Management Program--Laboratory Ser...

journal_title：Journal of clinical microbiology

authors： Desjardins M,Fleming CA

更新日期：2014-08-01 00:00:00

abstract：:Resistance to linezolid is rare in clinical isolates of Enterococcus faecalis. A strain resistant to this antimicrobial but susceptible to vancomycin was found to cause central venous catheter colonization in a patient who never received linezolid. ...

journal_title：Journal of clinical microbiology

authors： Marra AR,Major Y,Edmond MB

更新日期：2006-05-01 00:00:00

abstract：:We compared a set of commercial Salmonella somatic and flagellar serotyping antisera to in-house-prepared antisera from the Microbial Diseases Laboratory, California Department of Public Health, using 327 Salmonella enterica strains belonging to subgroups I, II, IIIa, IIIb, and IV. The sensitivities of Denka Seiken (T...

journal_title：Journal of clinical microbiology

authors： Schrader KN,Fernandez-Castro A,Cheung WK,Crandall CM,Abbott SL

更新日期：2008-02-01 00:00:00

abstract：:Influenza epidemics arise through the accumulation of viral genetic changes, culminating in a novel antigenic type that is able to escape host immunity. Following an outbreak of the A/Fujian/411/2002-like strains in Asia, including China, Japan, and South Korea, in 2002, Australia and New Zealand experienced substanti...

journal_title：Journal of clinical microbiology

authors： Kang S,Yang IS,Lee JY,Park Y,Oh HB,Kang C,Kim KH

更新日期：2010-06-01 00:00:00

abstract：:The fluorescent amplified-fragment length polymorphism (FAFLP) assay potentially amplifies a unique set of genome fragments from each bacterial clone. It uses stringently hybridizing primers which carry a fluorescent label. Precise fragment sizing is achieved by the inclusion of an internal size standard in every lane...

journal_title：Journal of clinical microbiology

authors： Arnold C,Metherell L,Willshaw G,Maggs A,Stanley J

更新日期：1999-05-01 00:00:00

abstract：:In comparative studies, Hemo-De (PMP Medical Industries, Inc., Irving, Tex.) was found to be a suitable replacement for ethyl acetate in the Formalin-ethyl acetate concentration technique. With essentially equivalent recovery rates for both procedures, the Formalin-Hemo-De concentration technique is considered to be t...

journal_title：Journal of clinical microbiology

authors： Neimeister R,Logan AL,Gerber B,Egleton JH,Kleger B

更新日期：1987-02-01 00:00:00

abstract：:We investigated the performance of a double-antigen sandwich recombinant enzyme immunoassay (EIA; Abbott Laboratories, North Chicago, Ill.) and compared it with that of a synthetic-peptide-based EIA (Biochem Immunosystems, Montreal, Quebec, Canada) for the detection of human immunodeficiency type 1 (HIV-1) and HIV-2 a...

journal_title：Journal of clinical microbiology

authors： Galli RA,Castriciano S,Fearon M,Major C,Choi KW,Mahony J,Chernesky M

更新日期：1996-04-01 00:00:00

abstract：:An increased human parvovirus B19 infection rate has been observed in immunocompromised hosts. In this study, we sought to determine the prevalence of parvovirus B19 infection in adult cancer patients receiving multiple courses of systemic chemotherapy. From March 1999 through April 2000, 59 men and 68 women, with a m...

journal_title：Journal of clinical microbiology

authors： Kuo SH,Lin LI,Chang CJ,Liu YR,Lin KS,Cheng AL

更新日期：2002-11-01 00:00:00

abstract：:A PCR method was developed to identify and fingerprint Candida krusei isolates simply and rapidly. The primer pair Arno1 and Arno2 was designed to amplify the polymorphic species-specific repetitive sequence CKRS-1 (C. krusei repeated sequence 1) that we identified in the nontranscribed intergenic regions (IGRs) of rR...

journal_title：Journal of clinical microbiology

authors： Carlotti A,Chaib F,Couble A,Bourgeois N,Blanchard V,Villard J

更新日期：1997-06-01 00:00:00

abstract：:Two DNA probes, one 1.1- and one 0.75-kb probe, specific for Serpulina hyodysenteriae were isolated from a genomic library generated from virulent S. hyodysenteriae 5380. These probes are highly specific and react with all S. hyodysenteriae strains tested. Under stringent conditions, the DNA probes did not react with ...

journal_title：Journal of clinical microbiology

authors： Sotiropoulos C,Smith SC,Coloe PJ

更新日期：1993-07-01 00:00:00

abstract：:Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spott...

journal_title：Journal of clinical microbiology

authors： Monecke S,Müller E,Buechler J,Rejman J,Stieber B,Akpaka PE,Bandt D,Burris R,Coombs G,Hidalgo-Arroyo GA,Hughes P,Kearns A,Abós SM,Pichon B,Skakni L,Söderquist B,Ehricht R

更新日期：2013-02-01 00:00:00

abstract：:Virology laboratories historically have used direct fluorescent-antibody assay (DFA) and culture to detect six or seven respiratory viruses. Following the discovery of five new human respiratory viruses since 2000, there is an increasing need for diagnostic tests to detect these emerging viruses. We have developed a n...

journal_title：Journal of clinical microbiology

authors： Mahony J,Chong S,Merante F,Yaghoubian S,Sinha T,Lisle C,Janeczko R

更新日期：2007-09-01 00:00:00

abstract：:Blastocystis hominis is now gaining acceptance as an agent of human intestinal disease. A case-control study of the cause of gastroenteritis in children less than 6 years old was conducted. A total of 500 stool specimens were examined by wet mount preparation, formalin-ether concentration, Sheather's sugar flotation t...

journal_title：Journal of clinical microbiology

authors： Nimri LF

更新日期：1993-10-01 00:00:00