Comparisons between direct microscopic and cultural methods for recognition of Corynebacterium vaginale in women with vaginitis.

abstract：:A rapid and highly sensitive real-time PCR detection and quantification method for infectious hypodermal and hematopoietic necrosis virus (IHHNV), a single-stranded DNA virus, and white spot virus (WSV), a double-stranded DNA (dsDNA) virus infecting penaeid shrimp (Penaeus sp.), was developed using the GeneAmp 5700 se...

journal_title：Journal of clinical microbiology

authors： Dhar AK,Roux MM,Klimpel KR

更新日期：2001-08-01 00:00:00

abstract：:Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain-Barré syndrome in adults. Diagnosis and surv...

journal_title：Journal of clinical microbiology

authors： Balmaseda A,Zambrana JV,Collado D,García N,Saborío S,Elizondo D,Mercado JC,Gonzalez K,Cerpas C,Nuñez A,Corti D,Waggoner JJ,Kuan G,Burger-Calderon R,Harris E

更新日期：2018-02-22 00:00:00

abstract：:By using a single synthetic oligonucleotide primer pair in the polymerase chain reaction, we amplified specific Shiga-like-toxin (SLT) gene segments from DNAs of 20 clinical Escherichia coli isolates, irrespective of whether they produce SLT-I, SLT-II, or heretofore uncategorized SLTs. These segments were not detectab...

journal_title：Journal of clinical microbiology

authors： Karch H,Meyer T

更新日期：1989-12-01 00:00:00

abstract：:Large hospital-based clinical laboratories must be prepared to rapidly investigate potential infectious disease outbreaks. To challenge the ability of our molecular diagnostics laboratory to use whole-genome sequencing in a potential outbreak scenario and identify impediments to these efforts, we studied 84 invasive s...

journal_title：Journal of clinical microbiology

authors： Olsen RJ,Fittipaldi N,Kachroo P,Sanson MA,Long SW,Como-Sabetti KJ,Valson C,Cantu C,Lynfield R,Van Beneden C,Beres SB,Musser JM

更新日期：2014-12-01 00:00:00

abstract：:Molecular techniques are playing an important role in the diagnosis of nontuberculous mycobacterial infections. This case report describes a chronic soft tissue infection in an immunocompetent patient caused by a previously undescribed pigmented, rapidly growing Mycobacterium species, emphasizing the importance of cli...

journal_title：Journal of clinical microbiology

authors： Turenne CY,Suchak AA,Wolfe JN,Kabani A,Nicolle LE

更新日期：2003-06-01 00:00:00

abstract：:A total of 221 isolates of multidrug-resistant Salmonella enterica serovar Typhimurium in Japan were characterized in the present study. The results revealed that clonal serovar Typhimurium definitive phage type 104 strains prevailed and that these strains had drug resistance patterns, integron types, and pulsed-field...

journal_title：Journal of clinical microbiology

authors： Izumiya H,Terajima J,Matsushita S,Tamura K,Watanabe H

更新日期：2001-07-01 00:00:00

abstract：:The ability of fluorescence resonance energy transfer, molecular-beacon, and TaqMan probes to detect single nucleotide polymorphism (SNP) in the presence of a wild-type allele was evaluated using drug resistance-conferring SNPs in mixed Mycobacterium tuberculosis DNA. It was found that both the absolute quantity and t...

journal_title：Journal of clinical microbiology

authors： Yesilkaya H,Meacci F,Niemann S,Hillemann D,Rüsch-Gerdes S,LONG DRUG Study Group.,Barer MR,Andrew PW,Oggioni MR

更新日期：2006-10-01 00:00:00

abstract：:Sera from patients with allergic bronchopulmonary aspergillosis (ABPA) or aspergilloma and normal sera were analyzed for specific antibodies by Western (immuno-) blotting with Aspergillus fumigatus antigens transferred electrophoretically onto polyvinylidene difluoride membranes. Western blot analysis demonstrated con...

journal_title：Journal of clinical microbiology

authors： Kurup VP,Greenberger PA,Fink JN

更新日期：1989-06-01 00:00:00

abstract：:Contamination of broth cultures of acid-fast bacilli (AFB) by bacterial species other than Mycobacterium species frequently occurs. Many of these contaminated cultures require redecontamination and reincubation before the appropriate tests can be performed for identification, significantly affecting the turnaround tim...

journal_title：Journal of clinical microbiology

authors： Zheng X,Pang M,Engler HD,Tanaka S,Reppun T

更新日期：2001-10-01 00:00:00

abstract：:Blastocystis hominis is now gaining acceptance as an agent of human intestinal disease. A case-control study of the cause of gastroenteritis in children less than 6 years old was conducted. A total of 500 stool specimens were examined by wet mount preparation, formalin-ether concentration, Sheather's sugar flotation t...

journal_title：Journal of clinical microbiology

authors： Nimri LF

更新日期：1993-10-01 00:00:00

abstract：:The production and characterization of five monoclonal antibodies to Mycobacterium tuberculosis are described. Specificity of the monoclonal antibodies was tested against other mycobacterial species by an enzyme-linked immunosorbent assay and immunoblots. HGT 3a, an immunoglobulin M (IgM) antibody, recognizes a molecu...

journal_title：Journal of clinical microbiology

authors： Kadival GV,Chaparas SD

更新日期：1987-01-01 00:00:00

abstract：:A directory of test patterns and their interpretations has been prepared for identification of Enterobacteriaceae by using the 11-test API 10 S kit. The diagnostic accuracy of the directory and kit were evaluated by using records of test results for 37,476 isolates studied with the 21-test API 20 Enteric kit. Analysis...

journal_title：Journal of clinical microbiology

authors： Robertson EA,MacLowry JD

更新日期：1975-06-01 00:00:00

abstract：:Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis, Entamoeba histolytica, Cryptosporidium parvum, and Cryptosporidium hominis Similarly, these species cause the majority of parasitic diarrhea acquired in the Un...

journal_title：Journal of clinical microbiology

authors： Madison-Antenucci S,Relich RF,Doyle L,Espina N,Fuller D,Karchmer T,Lainesse A,Mortensen JE,Pancholi P,Veros W,Harrington SM

更新日期：2016-11-01 00:00:00

abstract：:Random amplified polymorphic DNA (RAPD) was used to better characterize the genotypic relatedness among medically important Candida species. By using short oligomer primers (10-mers) with arbitrarily chosen sequences in the polymerase chain reaction, distinctive and reproducible sets of polymerase chain reaction produ...

journal_title：Journal of clinical microbiology

authors： Lehmann PF,Lin D,Lasker BA

更新日期：1992-12-01 00:00:00

abstract：:Pseudomonas gladioli was isolated from 11 patients with cystic fibrosis. It resembled Pseudomonas cepacia on the selective and differential medium OFPBL, producing yellow colonies after 48 to 72 h of incubation. Isolates were characterized biochemically, by DNA hybridization, and by cellular fatty acid analysis. A rev...

journal_title：Journal of clinical microbiology

authors： Christenson JC,Welch DF,Mukwaya G,Muszynski MJ,Weaver RE,Brenner DJ

更新日期：1989-02-01 00:00:00

abstract：:The diagnostic utility of Epstein-Barr virus (EBV) DNA detection in cerebrospinal fluid for the diagnosis of central nervous system lymphoma was evaluated with two different PCR assays to test a collection of cerebrospinal fluid samples from 24 AIDS patients with central nervous system disorders. A PCR assay amplifyin...

journal_title：Journal of clinical microbiology

authors： Arribas JR,Clifford DB,Fichtenbaum CJ,Roberts RL,Powderly WG,Storch GA

更新日期：1995-06-01 00:00:00

abstract：:A production model of the semi-automated PASCO MIC-ID system (PASCO Laboratories, Wheat Ridge, Colo.) was evaluated with 122 groups or species of gram-negative bacilli, which included typical (499 cultures) and atypical (37 cultures) strains of fermenters and nonfermenters. The PASCO system identified 90.9% of 536 cul...

journal_title：Journal of clinical microbiology

authors： Rhoden DL,Schable B,Smith PB

更新日期：1987-12-01 00:00:00

abstract：:We report the recovery of Histoplasma capsulatum from blood specimens cultured for Mycobacterium sp. in BACTEC 13A radiometric medium. H. capsulatum was recovered from six of eight blood specimens submitted for mycobacterial cultures from five human immunodeficiency virus-positive individuals. Initial positive metabol...

journal_title：Journal of clinical microbiology

authors： Merz WG,Kodsy S,Merz CS

更新日期：1992-01-01 00:00:00

abstract：:Serum samples collected from 68 patients (age, mean +/- the standard deviation [SD], 56.3 +/- 12.8 years) at admission who were subsequently molecularly diagnosed as having hepatitis E and from 2,781 individuals who were assumed not to have been recently infected with hepatitis E virus (HEV; negative controls; 52.9 +/...

journal_title：Journal of clinical microbiology

authors： Takahashi M,Kusakai S,Mizuo H,Suzuki K,Fujimura K,Masuko K,Sugai Y,Aikawa T,Nishizawa T,Okamoto H

更新日期：2005-01-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 (ST398) has frequently been detected in pigs and pig handlers. However, in Malaysia, sampling 360 pigs and 90 pig handlers from 30 farms identified novel ST9-spa type t4358-staphylococcal cassette chromosome mec type V MRSA strains that were found...

journal_title：Journal of clinical microbiology

authors： Neela V,Mohd Zafrul A,Mariana NS,van Belkum A,Liew YK,Rad EG

更新日期：2009-12-01 00:00:00

abstract：:Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct gram-negative bacteria. IEM should be a valuable tool for confirming presumptive isolates of F. tularensis and may potent...

journal_title：Journal of clinical microbiology

authors： Geisbert TW,Jahrling PB,Ezzell JW Jr

更新日期：1993-07-01 00:00:00

abstract：:Strains of Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, Mycobacterium xenopi, and Mycobacterium gordonae were identified by high-performance liquid chromatography (HPLC) analysis of mycolic acids as bromophenacyl esters. HPLC criteria were used to develop a flow chart identification s...

journal_title：Journal of clinical microbiology

authors： Butler WR,Thibert L,Kilburn JO

更新日期：1992-10-01 00:00:00

abstract：:The DNA fingerprint profiles and somatic serotypes of 71 Pasteurella multocida capsule serogroup B isolates, 13 capsule serogroup E isolates, and 16 somatic reference serotype strains were compared. Each of the 16 reference somatic serotypes had a unique DNA fingerprint profile with the HhaI restriction endonuclease. ...

journal_title：Journal of clinical microbiology

authors： Wilson MA,Rimler RB,Hoffman LJ

更新日期：1992-06-01 00:00:00

abstract：:A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this assay. Patients with the following IFI were selected according to the availability of sera obtained...

journal_title：Journal of clinical microbiology

authors： Cornu M,Sendid B,Mery A,François N,Mikulska M,Letscher-Bru V,De Carolis E,Damonti L,Titecat M,Bochud PY,Alanio A,Sanguinetti M,Viscoli C,Herbrecht R,Guerardel Y,Poulain D

更新日期：2019-04-26 00:00:00

abstract：:Nineteen multidrug-resistant (MDR) Mycobacterium complex strains isolated in a nosocomial outbreak were characterized at the molecular level. The strains were microbiologically characterized as Mycobacterium bovis. The mpt40 sequence was not present in chromosomal DNA from these strains, supporting the fact that they ...

journal_title：Journal of clinical microbiology

authors： Blázquez J,Espinosa de Los Monteros LE,Samper S,Martín C,Guerrero A,Cobo J,Van Embden J,Baquero F,Gómez-Mampaso E

更新日期：1997-06-01 00:00:00

abstract：:Hepatitis E virus (HEV) genotype 3 is endemic in Europe and hyperendemic in southern France. Recent reports of a high prevalence of HEV RNA in blood donations and in culinary specialties from this geographical area confirmed the endemicity of HEV and sources of viral transmission in this geographical area. HEV causes ...

journal_title：Journal of clinical microbiology

authors： Moal V,Legris T,Motte A,Vacher-Coponat H,Fages L,Jourde-Chiche N,Borentain P,Jaubert D,Gerolami R,Colson P

更新日期：2015-05-01 00:00:00

abstract：:From 1974 to 1994, 2,033 specimens from children were submitted for cultures for anaerobic bacteria. Eighty-three Veillonella spp. were recovered from 83 children (4%). Most Veillonella species were recovered from abscesses, aspiration pneumonias, burns, bites, and sinuses. The infections were polymicrobial in 79 (95%...

journal_title：Journal of clinical microbiology

authors： Brook I

更新日期：1996-05-01 00:00:00

abstract：:The recovery of Nocardia species from mixed cultures is facilitated by use of a selective medium. We show that buffered charcoal-yeast extract medium with polymyxin, anisomycin, and vancomycin can be used for the selective isolation of nocardiae from contaminated specimens. ...

journal_title：Journal of clinical microbiology

authors： Garrett MA,Holmes HT,Nolte FS

更新日期：1992-07-01 00:00:00

abstract：:Coxsackievirus (CV) strains CVA2, CVA4, CVA5, and CVA10 were isolated from patients with hand, foot, and mouth disease during a 2009 outbreak in China. Full genome sequences for four representative strains, CVA2/SD/CHN/09 (A2SD09), CVA4/SZ/CHN/09 (A4SZ09), CVA5/SD/CHN/09 (A5SD09), and CVA10/SD/CHN/09 (A10SD09), were d...

journal_title：Journal of clinical microbiology

authors： Hu YF,Yang F,Du J,Dong J,Zhang T,Wu ZQ,Xue Y,Jin Q

更新日期：2011-07-01 00:00:00

abstract：:The timely and exact diagnosis of prosthetic joint infection (PJI) is crucial for surgical decision-making. Intraoperatively, delivery of the result within an hour is required. Alpha-defensin lateral immunoassay of joint fluid (JF) is precise for the intraoperative exclusion of PJI; however, for patients with a limite...

journal_title：Journal of clinical microbiology

authors： Fillerova R,Gallo J,Radvansky M,Kraiczova V,Kudelka M,Kriegova E

更新日期：2017-09-01 00:00:00