Systematic serological testing for hepatitis E virus in kidney transplant recipients.

abstract：:The ability of Yersinia to digest polypectate may be of some value in differentiating Y. enterocolitica and Y. pseudotuberculosis from some of the other fermenting gram-negative bacilli, such as Enterobacter agglomerans, with which they can be confused. Pectolytic activity in Yersinia may also have some teleologic or ...

journal_title：Journal of clinical microbiology

authors： von Riesen VL

更新日期：1975-12-01 00:00:00

abstract：:A combination of immunomagnetic separation (IMS) and a polymerase chain reaction (PCR) procedure was used for direct isolation and identification of Shigella dysenteriae type 1 and Shigella flexneri from feces. Immunomagnetic particles were coated with monoclonal antibody MASFB, which is specific for a common epitope ...

journal_title：Journal of clinical microbiology

authors： Islam D,Lindberg AA

更新日期：1992-11-01 00:00:00

abstract：:The aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. One hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-...

journal_title：Journal of clinical microbiology

authors： Willinger B,Obradovic A,Selitsch B,Beck-Mannagetta J,Buzina W,Braun H,Apfalter P,Hirschl AM,Makristathis A,Rotter M

更新日期：2003-02-01 00:00:00

abstract：:Continuous cell lines were tested for their potential use in distinguishing pathogenic from nonpathogenic Listeria species. Listeria monocytogenes and Listeria ivanovii strains were lethal for mice, and culture filtrates were cytotoxic for cultured cells and hemolytic for sheep erythrocytes, while Listeria innocua, Li...

journal_title：Journal of clinical microbiology

authors： Farber JM,Speirs JI

更新日期：1987-08-01 00:00:00

abstract：:In an examination of arginine-utilizing Mycoplasma species, simple fluorogenic and chromogenic tests were used to demonstrate high levels of arginine aminopeptidase activity in unwashed, unconcentrated broth and agar cultures in all strains of 5 species and low levels of activity in all strains of 14 species. Four spe...

journal_title：Journal of clinical microbiology

authors： Ball HJ,Neill SD,Reid RL

更新日期：1985-05-01 00:00:00

abstract：:The Streptococcus bovis group has undergone significant taxonomic changes over the past 2 decades with the advent of new identification methods with higher discriminatory power. Although the current classification system is not yet embraced by all researchers in the field and debate remains over the performance of mol...

journal_title：Journal of clinical microbiology

authors： Dekker JP,Lau AF

更新日期：2016-07-01 00:00:00

abstract：:We report on the performance of a recently introduced commercial chessboard method using 12 antisera, in comparison with that of the 55-antiserum panel used in determining the serogroups and types (SGTs) of Streptococcus pneumoniae, both of which were carried out by a coagglutination technique. Of a total of 150 strai...

journal_title：Journal of clinical microbiology

authors： Lalitha MK,Thomas K,Kumar RS,Steinhoff MC

更新日期：1999-01-01 00:00:00

abstract：:VP1 sequences were determined for poliovirus type 1 isolates obtained over a 189-day period from a poliomyelitis patient with common variable immunodeficiency syndrome (a defect in antibody formation). The isolate from the first sample, taken 11 days after onset of paralysis, contained two poliovirus populations, diff...

journal_title：Journal of clinical microbiology

authors： Kew OM,Sutter RW,Nottay BK,McDonough MJ,Prevots DR,Quick L,Pallansch MA

更新日期：1998-10-01 00:00:00

abstract：:Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less t...

journal_title：Journal of clinical microbiology

authors： Hendry C,Dionne K,Hedgepeth A,Carroll K,Parrish N

更新日期：2009-04-01 00:00:00

abstract：:Entamoeba histolytica is an intestinal parasite causing significant morbidity and mortality worldwide. More tools are needed to understand the epidemiology and molecular pathogenesis of amebiasis. A cDNA library was constructed by using poly(A)+ RNA isolated from an axenic strain of E. histolytica, HM1:IMSS, which exp...

journal_title：Journal of clinical microbiology

authors： Burch DJ,Li E,Reed S,Jackson TF,Stanley SL Jr

更新日期：1991-04-01 00:00:00

abstract：:Nontuberculous mycobacteria (NTM) cause severe opportunistic infections and have a rising incidence in most settings. Rising diagnostic need must be met by national reference laboratories, which rely on Clinical and Laboratory Standards Institute (CLSI) guideline-approved manual readout of microtiter plates for antimi...

journal_title：Journal of clinical microbiology

authors： Rockland M,Ruth MM,Aalders N,Pennings L,Hoefsloot W,Wattenberg M,van Ingen J

更新日期：2019-03-28 00:00:00

abstract：:A nontoxigenic strain of Vibrio cholerae O group 1 was isolated in Florida from the stool of a patient with severe diarrhea. The strain had the same hemolytic and unique phage-sensitivity pattern as all toxigenic isolates from recent cases of cholera in Texas and Louisiana. Identical strains were transiently isolated ...

journal_title：Journal of clinical microbiology

authors： Morris JG Jr,Picardi JL,Lieb S,Lee JV,Roberts A,Hood M,Gunn RA,Blake PA

更新日期：1984-02-01 00:00:00

abstract：:Sixty-one Bordetella pertussis isolates were tested blindly in two laboratories to determine their serotype nature by monoclonal antibodies using two independent methods: the standard bacterial microagglutination assay and an indirect whole-cell enzyme-linked immunosorbent assay. Both methods gave concordant results i...

journal_title：Journal of clinical microbiology

authors： Tsang RS,Sill ML,Advani A,Xing D,Newland P,Hallander H

更新日期：2005-05-01 00:00:00

abstract：:Severe early childhood caries (ECC), while strongly associated with Streptococcus mutans using selective detection (culture, PCR), has also been associated with a widely diverse microbiota using molecular cloning approaches. The aim of this study was to evaluate the microbiota of severe ECC using anaerobic culture. Th...

journal_title：Journal of clinical microbiology

authors： Tanner AC,Mathney JM,Kent RL,Chalmers NI,Hughes CV,Loo CY,Pradhan N,Kanasi E,Hwang J,Dahlan MA,Papadopolou E,Dewhirst FE

更新日期：2011-04-01 00:00:00

abstract：:Streptococcus pyogenes, a major human pathogen, is still considered susceptible to beta-lactams, but for other relevant antibiotics, highly variable resistance rates have been reported. Since no data were available from Iran, we tested 1,335 throat isolates from two different regions of the country for their antibioti...

journal_title：Journal of clinical microbiology

authors： Jasir A,Tanna A,Noorani A,Mirsalehian A,Efstratiou A,Schalen C

更新日期：2000-06-01 00:00:00

abstract：:Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping r...

journal_title：Journal of clinical microbiology

authors： Moser SA,Mitchell SC,Ruby JD,Momeni S,Osgood RC,Whiddon J,Childers NK

更新日期：2010-02-01 00:00:00

abstract：:An isolate of Proteus mirabilis recovered from blood cultures of a diabetic patient was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole-cell and/or plasmid DNA recovered from the isolate with primers specific for the bla(KPC) carbapenemase ge...

journal_title：Journal of clinical microbiology

authors： Tibbetts R,Frye JG,Marschall J,Warren D,Dunne W

更新日期：2008-09-01 00:00:00

abstract：:Streptococcus pneumoniae is both a commensal and a major pathogen that causes invasive disease in people of all ages. The introduction of serotype-specific pneumococcal vaccines has reduced the burden of disease but has also led to replacement with new strains; thus, serotyping remains important for vaccine-related di...

journal_title：Journal of clinical microbiology

authors： Pholwat S,Sakai F,Turner P,Vidal JE,Houpt ER

更新日期：2016-07-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC) is an emerging enteric pathogen that causes acute and chronic diarrhea among children, human immunodeficiency virus-infected patients, and travelers to developing regions of the world. The pathogenesis of EAEC strains involves the production of biofilm. In this study, we deter...

journal_title：Journal of clinical microbiology

authors： Mohamed JA,Huang DB,Jiang ZD,DuPont HL,Nataro JP,Belkind-Gerson J,Okhuysen PC

更新日期：2007-01-01 00:00:00

abstract：:Of a total of 1043 macrolide-resistant Streptococcus pneumoniae isolates collected from 24 countries as part of PROTEKT 1999-2000, 71 isolates tested positive for both the mef(A) and erm(B) genes. Of 69 isolates subjected to further molecular investigations, all were resistant to tetracycline, 63 (91.3%) were resistan...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Morrissey I,Bakker S,Morris L,Buckridge S,Felmingham D

更新日期：2004-02-01 00:00:00

abstract：:The serological properties of six new pneumococcal capsular types are described. A table listing all 90 pneumococcal types and their cross-reactions is included. ...

journal_title：Journal of clinical microbiology

authors： Henrichsen J

更新日期：1995-10-01 00:00:00

abstract：:Genotypic typing by restriction fragment length polymorphism and pulsed-field gel electrophoresis showed that two neonates in a neonatal ward were infected with the same Campylobacter jejuni strain. Isolates from the mother and brother of the index patient were identical to each other but distinct from the neonatal ty...

journal_title：Journal of clinical microbiology

authors： Llovo J,Mateo E,Muñoz A,Urquijo M,On SL,Fernández-Astorga A

更新日期：2003-08-01 00:00:00

abstract：:A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency >/=5 x 10(-7) was established at >/=70 and >/=20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 microl of an...

journal_title：Journal of clinical microbiology

authors： Galán JC,Tato M,Baquero MR,Turrientes C,Baquero F,Martinez JL

更新日期：2004-09-01 00:00:00

abstract：:We compared the results of Gram staining and culture of cerebrospinal fluid to results obtained with a rapid PCR assay for the diagnosis of meningococcal meningitis in 281 cases of suspected bacterial meningitis. PCR had a sensitivity of 97% compared to a sensitivity of 55% for culture, and the PCR specificity was 99....

journal_title：Journal of clinical microbiology

authors： Richardson DC,Louie L,Louie M,Simor AE

更新日期：2003-08-01 00:00:00

abstract：:The aim of the present study was to evaluate the Helicobacter pylori ClariRes assay (Ingenetix, Vienna, Austria) for the detection of H. pylori infection and the simultaneous clarithromycin susceptibility testing of the H. pylori isolates in stool samples from 100 symptomatic children. The results obtained by this nov...

journal_title：Journal of clinical microbiology

authors： Lottspeich C,Schwarzer A,Panthel K,Koletzko S,Rüssmann H

更新日期：2007-06-01 00:00:00

abstract：:Vibrio cholerae serotype O1 organisms that do not produce cholera toxin and, in fact, lack the genetic material encoding the enterotoxin have recently been detected in coastal regions of the United States. Although these organisms have been assumed to be nonpathogenic, they have been considered a potential reservoir o...

journal_title：Journal of clinical microbiology

authors： Johnston JM,McFarland LM,Bradford HB,Caraway CT

更新日期：1983-05-01 00:00:00

abstract：:Quantitative real-time PCR for the detection of respiratory syncytial virus (RSV) RNA is increasingly used to study the causal role of RSV in lower airway disease. The objective of our study was to evaluate variations in RSV RNA loads at different steps in the RNA quantification process: (i) variation in RSV RNA load ...

journal_title：Journal of clinical microbiology

authors： van de Pol AC,Wolfs TF,van Loon AM,Tacke CE,Viveen MC,Jansen NJ,Kimpen JL,Rossen JW,Coenjaerts FE

更新日期：2010-10-01 00:00:00

abstract：:We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern Japanese prefecture of Nagasaki. The random amplified polymorphic DNA profiles obtained by using three primers revealed six pat...

journal_title：Journal of clinical microbiology

authors： Yamamoto Y,Kohno S,Koga H,Kakeya H,Tomono K,Kaku M,Yamazaki T,Arisawa M,Hara K

更新日期：1995-12-01 00:00:00

abstract：:The microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16S rRNA genes were randomly amplified by means of conserved eubacterial primers and cloned. Restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16S rRNA from the cult...

journal_title：Journal of clinical microbiology

authors： Dymock D,Weightman AJ,Scully C,Wade WG

更新日期：1996-03-01 00:00:00

abstract：:Methicillin-resistant coagulase-negative staphylococci were isolated from the nares and skin of 1- to 8-week-old healthy chickens in three flocks from a farm. Isolation of methicillin-resistant coagulase-negative staphylococci was positive for 72 (25.7%) of the 280 chickens tested, with the frequency varying from 2.2 ...

journal_title：Journal of clinical microbiology

authors： Kawano J,Shimizu A,Saitoh Y,Yagi M,Saito T,Okamoto R

更新日期：1996-09-01 00:00:00