Development of a TaqMan Array Card for Pneumococcal Serotyping on Isolates and Nasopharyngeal Samples.

abstract：:Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative ...

journal_title：Journal of clinical microbiology

authors： Titze-de-Almeida R,Willems RJ,Top J,Rodrigues IP,Ferreira RF 2nd,Boelens H,Brandileone MC,Zanella RC,Felipe MS,van Belkum A

更新日期：2004-10-01 00:00:00

abstract：:The genes encoding the 56-kDa polypeptides were amplified by polymerase chain reaction from the genomic DNAs of three serotypes of Rickettsia tsutsugamushi, Gilliam, Karp, and Boryong. The amplified products were cloned into expression vector pIH821, and the recombinant antigens were expressed in Escherichia coli as f...

journal_title：Journal of clinical microbiology

authors： Kim IS,Seong SY,Woo SG,Choi MS,Kang JS,Chang WH

更新日期：1993-08-01 00:00:00

abstract：:Studies of human immunodeficiency virus type 1 (HIV-1) transmission suggest that genital HIV-1 RNA and DNA may both be determinants of HIV-1 infectivity. Despite its potential role in HIV-1 transmission, there are limited quantitative data on genital HIV-1 DNA. Here we validated an in-house real-time PCR method for qu...

journal_title：Journal of clinical microbiology

authors： Benki S,McClelland RS,Emery S,Baeten JM,Richardson BA,Lavreys L,Mandaliya K,Overbaugh J

更新日期：2006-12-01 00:00:00

abstract：:Amphotericin B therapy continues to be the "gold standard" in the treatment of invasive aspergillosis in the immunocompromised host. Although Aspergillus fumigatus and Aspergillus flavus constitute the major species, several reports have described invasive pulmonary or disseminated disease due to the less common Asper...

journal_title：Journal of clinical microbiology

authors： Sutton DA,Sanche SE,Revankar SG,Fothergill AW,Rinaldi MG

更新日期：1999-07-01 00:00:00

abstract：:A DNA microarray platform, based on the nucleotide sequences of the internal transcribed spacer regions (ITS1 and ITS2) of the rRNA gene, was developed to identify 32 fungal pathogens at the species level. The probe sequences were spotted onto polycarbonate slides with a mini-microarray printer, and after the hybridiz...

journal_title：Journal of clinical microbiology

authors： Sturaro LL,Gonoi T,Busso-Lopes AF,Tararam CA,Levy CE,Lyra L,Trabasso P,Schreiber AZ,Kamei K,Moretti ML

更新日期：2018-04-25 00:00:00

abstract：:Chlamydia pneumoniae is a newly recognized organism associated with respiratory tract infections. Asymptomatic infection with C. pneumoniae, although it has been suggested to occur, has not been previously documented. We describe two asymptomatic individuals infected with this organism; these infections demonstrate th...

journal_title：Journal of clinical microbiology

authors： Hyman CL,Augenbraun MH,Roblin PM,Schachter J,Hammerschlag MR

更新日期：1991-09-01 00:00:00

abstract：:During 5.5 years of a study in Washington, D.C., hospitalizations of children for rotavirus gastroenteritis tended to be more common after a month of cold or dry weather than after a corresponding calendar month of warm or wet weather. Overall, there were 84% more (178 versus 97) inpatients with rotavirus gastroenteri...

journal_title：Journal of clinical microbiology

authors： Brandt CD,Kim HW,Rodriguez WJ,Arrobio JO,Jeffries BC,Parrott RH

更新日期：1982-09-01 00:00:00

abstract：:Helcococcus kunzii, a nonvirulent member of the human skin flora, has recently been implicated in causing infections in immunosuppressed patients. We report a case of breast abscess associated with H. kunzii in an immunocompetant patient and discuss the criteria used in its identification and our observations of susce...

journal_title：Journal of clinical microbiology

authors： Chagla AH,Borczyk AA,Facklam RR,Lovgren M

更新日期：1998-08-01 00:00:00

abstract：:Methicillin-resistant coagulase-negative staphylococci were isolated from the nares and skin of 1- to 8-week-old healthy chickens in three flocks from a farm. Isolation of methicillin-resistant coagulase-negative staphylococci was positive for 72 (25.7%) of the 280 chickens tested, with the frequency varying from 2.2 ...

journal_title：Journal of clinical microbiology

authors： Kawano J,Shimizu A,Saitoh Y,Yagi M,Saito T,Okamoto R

更新日期：1996-09-01 00:00:00

abstract：:We report the first documented case of endocarditis in a man infected with Bartonella alsatica, which causes bacteremia in healthy wild rabbits. B. alsatica was identified by serology and culture and by PCR of an aortic valve specimen. B. alsatica should be added to the list of zoonotic agents of blood culture-negativ...

journal_title：Journal of clinical microbiology

authors： Raoult D,Roblot F,Rolain JM,Besnier JM,Loulergue J,Bastides F,Choutet P

更新日期：2006-01-01 00:00:00

abstract：:Borrelia burgdorferi sensu lato species display considerable antigenic polymorphism. In order to evaluate the importance of this antigenic heterogeneity in the serodiagnosis of Lyme disease, the serum immunoglobulin G response in 148 healthy individuals from an area in northern Sweden where Lyme disease is endemic and...

journal_title：Journal of clinical microbiology

authors： Bunikis J,Olsén B,Westman G,Bergstroöm S

更新日期：1995-06-01 00:00:00

abstract：:To evaluate the accuracy and utility of the Eiken Systek No. 1 (Eiken system; Eiken Chemical Co., Ltd., Tokyo, Japan), we conducted a clinical comparison, with 345 Enterobacteriaceae isolates, of the Eiken System with API 20E (Analytab Products, Inc., Plainview, N.Y.) and conventional methods. The Eiken system is a 21...

journal_title：Journal of clinical microbiology

authors： Castillo CB,Bruckner DA

更新日期：1984-10-01 00:00:00

abstract：:Gastrointestinal disease and colonization by Helicobacter pylori were determined in 36 asymptomatic volunteers and 30 symptomatic individuals undergoing endoscopy and biopsy. Serum antibody immunoglobulin G (IgG) and IgA to H. pylori were measured by enzyme-linked immunosorbent assay. Serum antibody to a cytotoxin pro...

journal_title：Journal of clinical microbiology

authors： Leunk RD,Ferguson MA,Morgan DR,Low DE,Simor AE

更新日期：1990-06-01 00:00:00

abstract：:The FUNGITEST method (Sanofi Diagnostics Pasteur, Paris, France) is a microplate-based procedure for the breakpoint testing of six antifungal agents (amphotericin B, flucytosine, fluconazole, itraconazole, ketoconazole, and miconazole). We compared the FUNGITEST method with a broth microdilution test, performed accord...

journal_title：Journal of clinical microbiology

authors： Davey KG,Holmes AD,Johnson EM,Szekely A,Warnock DW

更新日期：1998-04-01 00:00:00

abstract：:Helicobacter pylori is known to be a major cause of peptic ulceration. The jhp0562 gene, encoding a glycosyltransferase involved in the synthesis of the lipopolysaccharide, was associated with peptic ulcer disease (PUD) in children. The beta-(1,3)-galactosyltransferase [beta-(1,3)GalT] gene (jhp0563), involved in Lewi...

journal_title：Journal of clinical microbiology

authors： Oleastro M,Santos A,Cordeiro R,Nunes B,Mégraud F,Ménard A

更新日期：2010-08-01 00:00:00

abstract：:Severe early childhood caries (ECC), while strongly associated with Streptococcus mutans using selective detection (culture, PCR), has also been associated with a widely diverse microbiota using molecular cloning approaches. The aim of this study was to evaluate the microbiota of severe ECC using anaerobic culture. Th...

journal_title：Journal of clinical microbiology

authors： Tanner AC,Mathney JM,Kent RL,Chalmers NI,Hughes CV,Loo CY,Pradhan N,Kanasi E,Hwang J,Dahlan MA,Papadopolou E,Dewhirst FE

更新日期：2011-04-01 00:00:00

abstract：:Chlamydia pecorum is a significant pathogen of domestic livestock and wildlife. We have developed a C. pecorum-specific multilocus sequence analysis (MLSA) scheme to examine the genetic diversity of and relationships between Australian sheep, cattle, and koala isolates. An MLSA of seven concatenated housekeeping gene ...

journal_title：Journal of clinical microbiology

authors： Jelocnik M,Frentiu FD,Timms P,Polkinghorne A

更新日期：2013-08-01 00:00:00

abstract：:Large hospital-based clinical laboratories must be prepared to rapidly investigate potential infectious disease outbreaks. To challenge the ability of our molecular diagnostics laboratory to use whole-genome sequencing in a potential outbreak scenario and identify impediments to these efforts, we studied 84 invasive s...

journal_title：Journal of clinical microbiology

authors： Olsen RJ,Fittipaldi N,Kachroo P,Sanson MA,Long SW,Como-Sabetti KJ,Valson C,Cantu C,Lynfield R,Van Beneden C,Beres SB,Musser JM

更新日期：2014-12-01 00:00:00

abstract：:Rickettsiae were isolated by cell culture of buffy coat blood from six patients with spotted fever from southeastern Australia and Flinders Island in Bass Strait. The isolates were genetically compared with two previous Rickettsia australis patient isolates. The genus-specific 17-kDA genes from the isolates were compa...

journal_title：Journal of clinical microbiology

authors： Baird RW,Stenos J,Stewart R,Hudson B,Lloyd M,Aiuto S,Dwyer B

更新日期：1996-06-01 00:00:00

abstract：:A severe outbreak of hemorrhagic colitis occurred in London, Ontario, during the month of September 1985. A total of 55 residents and 18 employees of a nursing home developed diarrhea, and 17 residents (age range, 78 to 99 years) died. Specimens from 38 patients, 37 employees and contacts, and 10 autopsies were invest...

journal_title：Journal of clinical microbiology

authors： Krishnan C,Fitzgerald VA,Dakin SJ,Behme RJ

更新日期：1987-06-01 00:00:00

abstract：:Central nervous system cysticercosis is common in countries where Taenia solium occurs in pigs and the level of hygiene and sanitation is low. The clinical presentation may include epileptic seizures, focal neurological deficits, hydrocephalus, or aseptic meningitis. The disease is frequently seen in California reside...

journal_title：Journal of clinical microbiology

authors： Mohammad IN,Heiner DC,Miller BL,Goldberg MA,Kagan IG

更新日期：1984-10-01 00:00:00

abstract：:Infections with human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2, respectively) are zoonotic infections. In Africa, the potential exists for additional cross-species transmissions from at least 33 different species of simian immunodeficiency virus (SIV)-infected nonhuman primates (NHPs) through hunting and ...

journal_title：Journal of clinical microbiology

authors： Ndongmo CB,Switzer WM,Pau CP,Zeh C,Schaefer A,Pieniazek D,Folks TM,Kalish ML

更新日期：2004-11-01 00:00:00

abstract：:The usefulness of filter paper for preservation of bacterial cells was shown by mixed-linker DNA fingerprint analysis of Mycobacterium tuberculosis isolates from 77 Brazilian patients. DNA fingerprints of samples spotted onto filter paper and conventional culture material were identical. Thus, filter paper specimens a...

journal_title：Journal of clinical microbiology

authors： Burger M,Raskin S,Brockelt SR,Amthor B,Geiss HK,Haas WH

更新日期：1998-02-01 00:00:00

abstract：:Opisthorchis viverrini is an important food-borne trematode in Southeast Asia. The infection causes significant morbidity in terms of hepatobiliary diseases and cholangiocarcinoma. The aim of this study was to improve the sensitivity of the PCR-based diagnosis of O. viverrini infection. A new fecal DNA extraction prot...

journal_title：Journal of clinical microbiology

authors： Duenngai K,Sithithaworn P,Rudrappa UK,Iddya K,Laha T,Stensvold CR,Strandgaard H,Johansen MV

更新日期：2008-01-01 00:00:00

abstract：:Antiserum specific for Salmonella O3 antigen was raised by immunization of rabbits with an artificial glycoconjugate consisting of the synthetic trisaccharide beta-D- Manp (1----4)-alpha-L- Rhap (1----3)-alpha-D-Galp covalently linked to bovine serum albumin (beta- MRG -BSA). Enzyme immunoassays showed that only lipop...

journal_title：Journal of clinical microbiology

authors： Ekwall E,Norberg T,Svenson SB,Lindberg AA

更新日期：1984-05-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤ 1.8 M, compared with a PCR assay that was functional at concentrations of <100 mM. The LAMP assay was as sensitive as the PCR ass...

journal_title：Journal of clinical microbiology

authors： Edwards T,Burke PA,Smalley HB,Gillies L,Hobbs G

更新日期：2014-06-01 00:00:00

abstract：:A reversed passive latex agglutination (RPLA) assay for determining the toxigenicity of Corynebacterium diphtheriae is presented. Rabbit antitoxin antiserum was raised by using commercially available diphtheria toxoid. This antiserum reacted with the diphtheria toxin when the culture supernatant was assayed by Western...

journal_title：Journal of clinical microbiology

authors： Toma C,Sisavath L,Iwanaga M

更新日期：1997-12-01 00:00:00

abstract：:We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpA), outer membrane protein (ompU), and central regulatory protein ToxR (toxR) in ...

journal_title：Journal of clinical microbiology

authors： Singh DV,Isac SR,Colwell RR

更新日期：2002-11-01 00:00:00

abstract：:The development of a novel normalized quantitative competitive real-time PCR on the LightCycler instrument (NQC-LC-PCR) and its application to the quantification of cytomegalovirus (CMV) DNA in clinical samples are described. A heterologous competitor DNA was spiked into test samples and served as an internal amplific...

journal_title：Journal of clinical microbiology

authors： Stöcher M,Berg J

更新日期：2002-12-01 00:00:00

abstract：:Staphylococcus aureus ST45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. It is one of the most prevalent carrier lineages but also frequently causes severe invasive disease, such as bacteremia. Little is known about its evolutionary history. In this study, we used whole-genome s...

journal_title：Journal of clinical microbiology

authors： Effelsberg N,Stegger M,Peitzmann L,Altinok O,Coombs GW,Pichon B,Kearns A,Randad PR,Heaney CD,Bletz S,Schaumburg F,Mellmann A

更新日期：2020-12-17 00:00:00