Asymptomatic respiratory tract infection with Chlamydia pneumoniae TWAR.

abstract：:We developed a highly specific, sensitive, and economical hemadsorption immunosorbent technique for the detection of dengue-specific immunoglobulin M (IgM) antibody. The technique is based on the reaction of human sera with anti-human IgM immobilized onto a solid phase followed by the detection of dengue-specific IgM ...

journal_title：Journal of clinical microbiology

authors： Gunasegaran K,Lim TW,Ahmed A,Aaskov JG,Lam SK,Pang T

更新日期：1986-01-01 00:00:00

abstract：:Simple sample-processing methods for PCR detection of Porphyromonas gingivalis, a major pathogen causing adult periodontitis, from saliva were studied. The ability to detect P. gingivalis from 118 salivary samples by PCR after boiling and Chelex 100 processing was compared with bacterial culture. P. gingivalis was det...

journal_title：Journal of clinical microbiology

authors： Mättö J,Saarela M,Alaluusua S,Oja V,Jousimies-Somer H,Asikainen S

更新日期：1998-01-01 00:00:00

abstract：:Sepsis is a major cause of death worldwide among nonhospitalized people and hospitalized patients. A wide range of pathogens are involved, and the correct identification and correct antimicrobial therapy are critical to ensure optimal clinical outcomes. With the recent introduction of matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Foster AG

更新日期：2013-11-01 00:00:00

abstract：:The genetic compositions of 71 isoniazid-resistant Mycobacterium tuberculosis strains from Poland were determined by spoligotyping. Nearly 80% of the isolates belonged to either the T or the Haarlem family. The genotypic diversity was largely due to variation within those families. The scarcity of imported genotypes s...

journal_title：Journal of clinical microbiology

authors： Augustynowicz-Kopec E,Jagielski T,Zwolska Z

更新日期：2008-12-01 00:00:00

abstract：:The emergence of ciprofloxacin-resistant Salmonella enterica serovar Choleraesuis in recent years has become an important public health issue in Taiwan. The resistant strains that cause human infections are considered to be from pigs. In this study, we characterized 157 swine and 42 human Salmonella serovar Choleraesu...

journal_title：Journal of clinical microbiology

authors： Chang CC,Lin YH,Chang CF,Yeh KS,Chiu CH,Chu C,Chien MS,Hsu YM,Tsai LS,Chiou CS

更新日期：2005-06-01 00:00:00

abstract：:HIV-1 non-B subtypes/circulating recombinant forms (CRFs) are increasing worldwide. Since subtype identification can be clinically relevant, we assessed the added value in HIV-1 subtyping using updated molecular phylogeny (Mphy) and the performance of routinely used automated tools. Updated Mphy (2015 updated referenc...

journal_title：Journal of clinical microbiology

authors： Fabeni L,Berno G,Fokam J,Bertoli A,Alteri C,Gori C,Forbici F,Takou D,Vergori A,Zaccarelli M,Maffongelli G,Borghi V,Latini A,Pennica A,Mastroianni CM,Montella F,Mussini C,Andreoni M,Antinori A,Perno CF,Santoro MM,

更新日期：2017-09-01 00:00:00

abstract：:We describe a multiplex PCR assay to identify and discriminate between isolates of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. The C. jejuni isolate F38011 lpxA gene, encoding a UDP-N-acetylglucosamine acyltransferase, was identified by sequence analysis of an expressio...

journal_title：Journal of clinical microbiology

authors： Klena JD,Parker CT,Knibb K,Ibbitt JC,Devane PM,Horn ST,Miller WG,Konkel ME

更新日期：2004-12-01 00:00:00

abstract：:This report describes an unusual pathogen associated with post-traumatic meningitis. Haemophilus influenzae type D was isolated from both cerebrospinal fluid and blood of this patient with meningitis. ...

journal_title：Journal of clinical microbiology

authors： Viner JP,Massanari RM

更新日期：1979-02-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome has been devastating the swine industry since the late 1980s. The disease has been controlled, to some extent, through the use of modified live-attenuated (MLV) vaccines once available. However, such a practice periodically resulted in isolation or detection of vaccine-lik...

journal_title：Journal of clinical microbiology

authors： Key KF,Guenette DK,Yoon KJ,Halbur PG,Toth TE,Meng XJ

更新日期：2003-06-01 00:00:00

abstract：:Bordetella pertussis was transmitted from an immunized boy to his father and possibly to other family members. This case report demonstrates that although unusual, acute adult B. pertussis infection can occur. B. pertussis immunization may not prevent infection but can reduce the severity. B. pertussis was detected in...

journal_title：Journal of clinical microbiology

authors： Smith S,Tilton RC

更新日期：1996-02-01 00:00:00

abstract：:Colony phenotype and genetic similarity were assessed within and between groups of commensal and pathogenic strains of Candida albicans collected from the oral cavities of individuals in a single geographical locale. Thirty-eight percent of pathogenic isolates contained predominant or minor variant colony morphologies...

journal_title：Journal of clinical microbiology

authors： Hellstein J,Vawter-Hugart H,Fotos P,Schmid J,Soll DR

更新日期：1993-12-01 00:00:00

abstract：:A dot immunobinding assay was used to detect antibodies to measles virus, mumps virus, and rubella virus antigens. Filter paper soaked with serum or whole blood was directly applied to the antigen-coated nitrocellulose sheets. The test was easy to perform, and its results agreed very well with those obtained by standa...

journal_title：Journal of clinical microbiology

authors： Condorelli F,Ziegler T

更新日期：1993-03-01 00:00:00

abstract：:Collecting whole blood on filter paper simplifies the processing, transport, and storage of specimens used for the diagnosis of human immunodeficiency virus type 1 (HIV-1) and other tests. Specimens may be collected in tropical or rural areas with minimal facilities for handling specimens. To compare simulated tropica...

journal_title：Journal of clinical microbiology

authors： Mitchell C,Jennings C,Brambilla D,Aldrovandi G,Amedee AM,Beck I,Bremer JW,Coombs R,Decker D,Fiscus S,Fitzgibbon J,Luzuriaga K,Moye J,Palumbo P,Reichelderfer P,Somasundaran M,Stevens W,Frenkel L,Dried Blood Spot Workin

更新日期：2008-09-01 00:00:00

abstract：:Paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (Dasypus novemcinctus) were comparable with 19 clinical isolates by sequence analysis of the PbGP43 gene and ribosomal internal transcribed spacer 1 (ITS1) and ITS2 and by random amplified polymorphic DNA. In this original ITS study, eight isolates ...

journal_title：Journal of clinical microbiology

authors： Hebeler-Barbosa F,Morais FV,Montenegro MR,Kuramae EE,Montes B,McEwen JG,Bagagli E,Puccia R

更新日期：2003-12-01 00:00:00

abstract：:Meningococcal disease is normally suspected on clinical grounds and is confirmed by isolation of Neisseria meningitidis bacteria from blood or cerebrospinal fluid or, more recently, by serology or PCR of cerebrospinal fluid. Achieving confirmation of a clinical diagnosis of meningococcal disease has become more diffic...

journal_title：Journal of clinical microbiology

authors： Newcombe J,Cartwright K,Palmer WH,McFadden J

更新日期：1996-07-01 00:00:00

abstract：:Disseminated Mycobacterium avium infection is a common complication in late-stage AIDS. We describe a patient seropositive for human immunodeficiency virus with a disseminated infection caused by mycobacteria. Acid-fast rods were visible by microscopy in stool and bone marrow and in specimens from skin, liver, spleen,...

journal_title：Journal of clinical microbiology

authors： Kirschner P,Vogel U,Hein R,Böttger EC

更新日期：1994-03-01 00:00:00

abstract：:We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobul...

journal_title：Journal of clinical microbiology

authors： Woo PC,Lau SK,Wong BH,Tsoi HW,Fung AM,Chan KH,Tam VK,Peiris JS,Yuen KY

更新日期：2004-05-01 00:00:00

abstract：:Group A streptococcus (GAS) species cause bacterial pharyngitis in both adults and children. Early and accurate diagnosis of GAS is important for appropriate antibiotic therapy to prevent GAS sequalae. The Revogene Strep A molecular assay (Meridian Bioscience Canada Inc, Quebec City, QC, Canada) is an automated real-t...

journal_title：Journal of clinical microbiology

authors： Banerjee D,Michael J,Schmitt B,Salimnia H,Mhaissen N,Goldfarb DM,Lachance P,Faron ML,Aufderheide T,Ledeboer N,Weissfeld A,Selvarangan R

更新日期：2020-06-24 00:00:00

abstract：:It has been difficult to obtain pure Pneumocystis carinii antigen either from cultures or from infected lungs for use in producing a specific antibody against P. carinii. This report describes an approach toward producing a monoclonal antibody that bypasses the antigen purification steps. P. carinii infection was deve...

journal_title：Journal of clinical microbiology

authors： Lee CH,Bolinger CD,Bartlett MS,Kohler RB,Wilde CE 3rd,Smith JW

更新日期：1986-03-01 00:00:00

abstract：:Tremendous interest has been generated in the commercial kits now available that incorporate herpes simplex virus isolation in cell culture with immunoperoxidase staining for viral antigen detection. Most studies comparing commercial kits with conventional cell culture techniques have found the kits to be less sensiti...

journal_title：Journal of clinical microbiology

authors： Zhao LS,Landry ML,Balkovic ES,Hsiung GD

更新日期：1987-08-01 00:00:00

abstract：:Chromosomal DNAs of selected Bacteroides organisms whose relatedness had been previously determined by "conventional" filter-annealing studies (J. L. Johnson, Int. J. Syst. Bacteriol. 28:245, 1978) were further analyzed by restriction endonuclease analysis coupled with the Southern hybridization procedure (E. M. South...

journal_title：Journal of clinical microbiology

authors： Bradbury WC,Murray RG,Mancini C,Morris VL

更新日期：1985-01-01 00:00:00

abstract：:Thirty stool filtrates known to contain Clostridium difficile toxin based on previous testing on McCoy cells were tested for toxicity on primary African green monkey kidney (AGMK), McCoy, MRC-5, primary rhesus monkey kidney (RMK), and Vero cells. All 30 filtrates showed cytotoxic effect at greater than or equal to 1:1...

journal_title：Journal of clinical microbiology

authors： Maniar AC,Williams TW,Hammond GW

更新日期：1987-10-01 00:00:00

abstract：:Infections due to biochemically typical Yersinia enterocolitica usually present as gastroenteritis, mesenteric lymphadenitis, terminal ileitis, and septicemia often with visceral abscesses. In these instances, the isolates have been biochemically typical and of well-established serotypes, namely 0:3 or 0:9 and, in the...

journal_title：Journal of clinical microbiology

authors： Bottone EJ

更新日期：1978-06-01 00:00:00

abstract：:A sensitive PCR assay that detects the thermophilic campylobacters C. jejuni, C. coli, C. lari, and C. upsaliensis is reported. Furthermore, by digestion of the PCR products with two restriction enzymes, species differentiation was demonstrated. Thus, the present method has the potential to be used for both detection ...

journal_title：Journal of clinical microbiology

authors： Fermér C,Engvall EO

更新日期：1999-10-01 00:00:00

abstract：:A screening assay for phospholipase C using a chromogenic substrate incorporated into agar medium is described. The assay directly visualizes phospholipase C activity of mycoplasma lysates and membranes on agar plates, or the activity may be measured by spectrophotometry. The results from the assay confirm the presenc...

journal_title：Journal of clinical microbiology

authors： De Silva NS,Quinn PA

更新日期：1987-04-01 00:00:00

abstract：:Nasopharyngeal secretions from adults and children were obtained in Stockholm, Sweden, for routine diagnosis of influenza A virus, influenza B virus, respiratory syncytial (RS) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. The cells in nasoph...

journal_title：Journal of clinical microbiology

authors： Grandien M,Pettersson CA,Gardner PS,Linde A,Stanton A

更新日期：1985-11-01 00:00:00

abstract：:Cell-free and cell-associated human immunodeficiency virus cultures suspended in 10% serum remained infectious for several weeks at room temperature. The stability was further increased when cell-associated virus was suspended in neat serum. When dried onto a glass coverslip, virus remained infectious for several days...

journal_title：Journal of clinical microbiology

authors： van Bueren J,Simpson RA,Jacobs P,Cookson BD

更新日期：1994-02-01 00:00:00

abstract：:The highly specific and sensitive latex agglutination test for cryptococcal antigen detection in cerebrospinal fluid is routine in many hospitals. Contamination of cerebrospinal fluid by a minute amount of syneresis fluid (surface condensation) from agar gave a strongly positive reaction which was heat stable, was not...

journal_title：Journal of clinical microbiology

authors： Boom WH,Piper DJ,Ruoff KL,Ferraro MJ

更新日期：1985-11-01 00:00:00

abstract：:We describe a 2009 H1N1 virus infection with a high viral load in a previously healthy infant who presented with complex febrile seizures and improved on oseltamivir without neurologic sequelae. Febrile seizures may be a complication in young children experiencing infection with high viral loads of 2009 H1N1 influenza...

journal_title：Journal of clinical microbiology

authors： O'Leary MF,Chappell JD,Stratton CW,Cronin RM,Taylor MB,Tang YW

更新日期：2010-10-01 00:00:00

abstract：:We compared the Gen-Probe transcription-mediated amplification assay (AMP CT), the Abbott LCx assay, and the Roche COBAS AMPLICOR assay for the detection of Chlamydia trachomatis in a mixed population in urine samples. First-void urine, urethral specimens, and cervical specimens in females were obtained from 1,000 pat...

journal_title：Journal of clinical microbiology

authors： Goessens WH,Mouton JW,van der Meijden WI,Deelen S,van Rijsoort-Vos TH,Lemmens-den Toom N,Verbrugh HA,Verkooyen RP

更新日期：1997-10-01 00:00:00