Evaluation of a rapid PCR assay for diagnosis of meningococcal meningitis.

abstract：:We undertook a controlled study of children younger than 5 years in Bangladesh to determine whether enterotoxigenic Bacteroides fragilis (ETBF) was associated with diarrhea in this population. ETBF was isolated from 22 (6.1%) of 358 patients and 5 (1.2%) of 425 controls (P = 0.0001). In children younger than 1 year, h...

journal_title：Journal of clinical microbiology

authors： Sack RB,Albert MJ,Alam K,Neogi PK,Akbar MS

更新日期：1994-04-01 00:00:00

abstract：:MICs and minimal bactericidal concentrations (MBCs) were evaluated for the four antibiotics azithromycin, amoxicillin, ceftriaxone, and doxycycline against the three main genospecies of Borrelia burgdorferi sensu lato. In MBC testing, statistically significant differences between the genospecies could be found in 7 ou...

journal_title：Journal of clinical microbiology

authors： Sicklinger M,Wienecke R,Neubert U

更新日期：2003-04-01 00:00:00

abstract：:The diagnosis and management of pneumonia are limited by the use of culture-based techniques of microbial identification, which may fail to identify unculturable, fastidious, and metabolically active viable but unculturable bacteria. Novel high-throughput culture-independent techniques hold promise but have not been s...

journal_title：Journal of clinical microbiology

authors： Dickson RP,Erb-Downward JR,Prescott HC,Martinez FJ,Curtis JL,Lama VN,Huffnagle GB

更新日期：2014-10-01 00:00:00

abstract：:The recovery of Nocardia species from mixed cultures is facilitated by use of a selective medium. We show that buffered charcoal-yeast extract medium with polymyxin, anisomycin, and vancomycin can be used for the selective isolation of nocardiae from contaminated specimens. ...

journal_title：Journal of clinical microbiology

authors： Garrett MA,Holmes HT,Nolte FS

更新日期：1992-07-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is known to cause respiratory tract infections in children and immunocompromised individuals. Given the difficulties of identifying hMPV by conventional culture, molecular techniques could improve the detection of this virus in clinical specimens...

journal_title：Journal of clinical microbiology

authors： Dare R,Sanghavi S,Bullotta A,Keightley MC,George KS,Wadowsky RM,Paterson DL,McCurry KR,Reinhart TA,Husain S,Rinaldo CR

更新日期：2007-02-01 00:00:00

abstract：:We compared putative molecular markers of virulence (vacA, cagA, and iceA) of Helicobacter pylori strains isolated from 52 adult duodenal ulcer patients from West Bengal, India, with those of H. pylori strains isolated from 48 adult healthy volunteers from the same region. On the basis of genotyping by PCR, we conclud...

journal_title：Journal of clinical microbiology

authors： Chattopadhyay S,Datta S,Chowdhury A,Chowdhury S,Mukhopadhyay AK,Rajendran K,Bhattacharya SK,Berg DE,Nair GB

更新日期：2002-07-01 00:00:00

abstract：:Parameters like zone reading, inoculum density, and plate streaking influence the precision and accuracy of disk diffusion antibiotic susceptibility testing (AST). While improved reading precision has been demonstrated using automated imaging systems, standardization of the inoculum and of plate streaking have not bee...

journal_title：Journal of clinical microbiology

authors： Hombach M,Maurer FP,Pfiffner T,Böttger EC,Furrer R

更新日期：2015-12-01 00:00:00

abstract：:In accordance with recent WHO recommendations, this study evaluates the sensitivities of PCR and microscopy for fine-needle aspiration (FNA) versus techniques involving swabs and punch biopsy specimens and suggests that FNA can replace punch biopsies for nonulcerative lesions and may serve as an alternative for ulcera...

journal_title：Journal of clinical microbiology

authors： Herbinger KH,Beissner M,Huber K,Awua-Boateng NY,Nitschke J,Thompson W,Klutse E,Agbenorku P,Assiobo A,Piten E,Wiedemann F,Fleischmann E,Helfrich K,Adjei O,Löscher T,Bretzel G

更新日期：2010-10-01 00:00:00

abstract：:Cryptococcus gattii emerged in North America in 1999 as a human and veterinary pathogen on Vancouver Island, British Columbia. The emergent subtype, VGIIa, and the closely related subtype VGIIb can now be found in the United States in Washington, Oregon, and California. We performed multilocus sequence typing and anti...

journal_title：Journal of clinical microbiology

authors： Iqbal N,DeBess EE,Wohrle R,Sun B,Nett RJ,Ahlquist AM,Chiller T,Lockhart SR,Cryptococcus gattii Public Health Working Group.

更新日期：2010-02-01 00:00:00

abstract：:A new test principle for the simultaneous detection of total approximate titers and immunoglobulin M antibodies has been developed and applied to the detection of antibody to hepatitis B core antigen. The method is based on the combination of a competition radioimmunoassay, for the determination of total antibody tite...

journal_title：Journal of clinical microbiology

authors： Angarano G,Monno L,Santantonio TA,Pastore G

更新日期：1984-06-01 00:00:00

abstract：:A total of 186 volunteers, including 40 hospital patients, participated in a cross-sectional survey of oropharyngeal colonization with Legionella pneumophila. Colonization was defined as the appearance of any L. pneumophila organisms on culture or a positive direct fluorescent-antibody (FA) test or both in the absence...

journal_title：Journal of clinical microbiology

authors： Bridge JA,Edelstein PH

更新日期：1983-11-01 00:00:00

abstract：:We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Prudent E,Gautret P,Memish ZA,Raoult D

更新日期：2015-03-01 00:00:00

abstract：:A new molecular assay, based on a rapid DNA extraction protocol, PCR, and hybridization to a specific probe in a nonradioactive microwell plate format was used to detect Mycoplasma pneumoniae in bronchoalveolar fluid specimens. The sensitivity of the assay was determined to be 10 to 100 organisms with M. pneumoniae re...

journal_title：Journal of clinical microbiology

authors： Kessler HH,Dodge DE,Pierer K,Young KK,Liao Y,Santner BI,Eber E,Roeger MG,Stuenzner D,Sixl-Voigt B,Marth E

更新日期：1997-06-01 00:00:00

abstract：:psaA is the gene encoding pneumococcal surface adhesin A (PsaA), a 37-kDa protein expressed on the surface of Streptococcus pneumoniae. PCR primers for psaA have been shown to amplify the target DNA sequence in all 90 serotypes of S. pneumoniae and in none of 67 heterologous pathogens and colonizing bacteria of the up...

journal_title：Journal of clinical microbiology

authors： Scott JA,Marston EL,Hall AJ,Marsh K

更新日期：2003-06-01 00:00:00

abstract：:The incidence of enterotoxigenic Escherichia coli (ETEC) was investigated in 95 E. coli strains isolated from 48 infants with diarrhea in Santiago, Chile. By using standard biological assays and DNA-DNA hybridization procedures, ETEC was found in 31.2% of the cases: 14 strains produced heat-stable enterotoxin (ST) onl...

journal_title：Journal of clinical microbiology

authors： Agüero ME,Reyes L,Prado V,Orskov I,Orskov F,Cabello FC

更新日期：1985-10-01 00:00:00

abstract：:Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Epidemiological studies of GBS infections require comprehensive typing systems that provide informa...

journal_title：Journal of clinical microbiology

authors： Sun Y,Kong F,Zhao Z,Gilbert GL

更新日期：2005-09-01 00:00:00

abstract：:Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was d...

journal_title：Journal of clinical microbiology

authors： Vannier AM,Tarrand JJ,Murray PR

更新日期：1988-09-01 00:00:00

abstract：:Murine monoclonal antibodies (MAbs) were produced which were specific for Pasteurella haemolytica serotype 1 lipopolysaccharide (LPS). The MAbs also reacted with LPS present in a partially purified antigen derived from a saline extract of the organism. The epitope to which the MAbs were directed was a carbohydrate whi...

journal_title：Journal of clinical microbiology

authors： Durham JA,Antone SM,Cunningham MW,Confer AW

更新日期：1988-05-01 00:00:00

abstract：:We have developed a method for determining the serotypes of poliovirus isolates by PCR. Three sets of serotype-specific antisense PCR-initiating primers (primers seroPV1A, seroPV2A, and seroPV3A) were designed to pair with codons of VP1 amino acid sequences that are conserved within but that differ across serotypes. T...

journal_title：Journal of clinical microbiology

authors： Kilpatrick DR,Nottay B,Yang CF,Yang SJ,Da Silva E,Peñaranda S,Pallansch M,Kew O

更新日期：1998-02-01 00:00:00

abstract：:Rose Bengal and counterimmunoelectrophoresis, two tests that detect antibodies against different structural antigens, when carried out on spinal fluid permitted rapid diagnosis of human Brucella meningitis. The Rose Bengal test was positive in five out of five patients studied, and counterimmunoelectrophoresis was pos...

journal_title：Journal of clinical microbiology

authors： Díaz R,Maraví-Poma E,Delgado G,Rivero A

更新日期：1978-02-01 00:00:00

abstract：:The ImmunoCard STAT! Cryptosporidium/Giardia rapid assay (Meridian Bioscience, Inc.) is a solid-phase qualitative immunochromatographic assay that detects and distinguishes between Giardia lamblia and Cryptosporidium parvum in aqueous extracts of human fecal specimens (fresh, frozen, unfixed, or fixed in 5 or 10% form...

journal_title：Journal of clinical microbiology

authors： Garcia LS,Shimizu RY,Novak S,Carroll M,Chan F

更新日期：2003-01-01 00:00:00

abstract：:HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific f...

journal_title：Journal of clinical microbiology

authors： Estrade C,Sahli R

更新日期：2014-11-01 00:00:00

abstract：:Blood isolates of Salmonella enterica serovar Typhi from two recently returned Bangladeshi patients in Kuwait were ciprofloxacin resistant, with ciprofloxacin MICs of 12 mg/liter for both isolates. Both isolates had three novel gyrA mutations (55-Leu-->Trp, 87-Asp-->Ala, and 106-Gln-->Arg) and three novel parC mutatio...

journal_title：Journal of clinical microbiology

authors： Dimitrov T,Dashti AA,Albaksami O,Udo EE,Jadaon MM,Albert MJ

更新日期：2009-01-01 00:00:00

abstract：:In a long-term rodent bioassay evaluating the carcinogenicity of triethanolamine, there was equivocal evidence of carcinogenic activity in male B6C3F1 mice, based on a marginal increase in the number of hepatocellular adenomas and hepatoblastomas. Interpretation was complicated by the presence of Helicobacter hepaticu...

journal_title：Journal of clinical microbiology

authors： Fox JG,MacGregor JA,Shen Z,Li X,Lewis R,Dangler CA

更新日期：1998-05-01 00:00:00

abstract：:Recent studies suggest that a group of Chlamydia strains known as TWAR, which are now proposed to be a new species called Chlamydia pneumoniae, may be a frequent cause of respiratory disease in the United States and many other countries. Current serotesting methods do not allow rapid screening of large numbers of samp...

journal_title：Journal of clinical microbiology

authors： Ladany S,Black CM,Farshy CE,Ossewaarde JM,Barnes RC

更新日期：1989-12-01 00:00:00

abstract：:In a multi-health center study, a new rapid optical immunoassay (OIA) for the detection of Shiga toxin types 1 and 2, the BioStar OIA SHIGATOX kit (Inverness Medical Professional Diagnostics, Inc.), was used to prospectively screen 742 fresh fecal samples for Shiga toxins in parallel with the Premier enterohemorrhagic...

journal_title：Journal of clinical microbiology

authors： Teel LD,Daly JA,Jerris RC,Maul D,Svanas G,O'Brien AD,Park CH

更新日期：2007-10-01 00:00:00

abstract：:By using the computer-assisted Dendron system to analyze the patterns of Southern blots probed with the repetitive sequence Ca3, we have compared oral isolates of Candida spp. from a group of 11 nonhospitalized patients with AIDS suffering from recurrent episodes of oral thrush in Leicester, England, with oral isolate...

journal_title：Journal of clinical microbiology

authors： Schmid J,Odds FC,Wiselka MJ,Nicholson KG,Soll DR

更新日期：1992-04-01 00:00:00

abstract：:Streptococcus pneumoniae is both a commensal and a major pathogen that causes invasive disease in people of all ages. The introduction of serotype-specific pneumococcal vaccines has reduced the burden of disease but has also led to replacement with new strains; thus, serotyping remains important for vaccine-related di...

journal_title：Journal of clinical microbiology

authors： Pholwat S,Sakai F,Turner P,Vidal JE,Houpt ER

更新日期：2016-07-01 00:00:00

abstract：:We compared whole blood dried on filter paper to the standard assay with frozen cell-free plasma for use in the quantitation of the human immunodeficiency virus RNA load in blood. RNA values from filter paper, corrected for the hematocrit, gave results comparable to those of the standard assay in terms of sensitivity ...

journal_title：Journal of clinical microbiology

authors： Fiscus SA,Brambilla D,Grosso L,Schock J,Cronin M

更新日期：1998-01-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection is an increasing health problem worldwide. Quantitative assays for HCV viral load are valuable in predicting response to therapy and for following treatment efficacy. Unfortunately, most quantitative tests for HCV RNA are limited by poor sensitivity. We have developed a convenient, hi...

journal_title：Journal of clinical microbiology

authors： Cook L,Ng KW,Bagabag A,Corey L,Jerome KR

更新日期：2004-09-01 00:00:00