Mycobacterial cross contamination during radiometric culturing.

abstract：:Concentrations of Mycobacterium avium-M. intracellulare ranging from 10(-1) to 10(3) CFU/ml were added to blood, placed in Isolator tubes, and held at room temperature for intervals ranging from 4 h to 56 days before being processed (centrifugation and culture on Middlebrook 7H10 agar). At all concentrations tested, M...

journal_title：Journal of clinical microbiology

authors： von Reyn CF,Hennigan S,Niemczyk S,Jacobs NJ

更新日期：1991-06-01 00:00:00

abstract：:To determine that susceptibility of AMPLICOR Chlamydia trachomatis PCR to inhibitory factors possibly present in cervical specimens, we obtained cervical specimens from 200 gynecology patients attending our outpatient clinic. The prevalence of C. trachomatis infection was 4.1%, as determined by cell culture. All AMPLI...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Luijendijk A,Huisman WM,Goessens WH,Kluytmans JA,van Rijsoort-Vos JH,Verbrugh HA

更新日期：1996-12-01 00:00:00

abstract：:We enrolled 22 patients with gas-forming pyogenic liver abscess in a study to assess the mechanism of gas formation. Klebsiella pneumoniae was cultured from specimens from all patients. Gas and pus samples from abscesses revealed four major components: nitrogen, oxygen, carbon dioxide, and hydrogen; this implicates mi...

journal_title：Journal of clinical microbiology

authors： Lee HL,Lee HC,Guo HR,Ko WC,Chen KW

更新日期：2004-06-01 00:00:00

abstract：:Recent studies suggest that a group of Chlamydia strains known as TWAR, which are now proposed to be a new species called Chlamydia pneumoniae, may be a frequent cause of respiratory disease in the United States and many other countries. Current serotesting methods do not allow rapid screening of large numbers of samp...

journal_title：Journal of clinical microbiology

authors： Ladany S,Black CM,Farshy CE,Ossewaarde JM,Barnes RC

更新日期：1989-12-01 00:00:00

abstract：:A newly developed commercial serological test (Iatron Laboratories, Inc., Tokyo, Japan) for the rapid identification of medically important species of Candida was evaluated against the API 20C (Analytab Products, Plainview, N.Y.) and the standard Wickerham assimilation and fermentation procedures. Our results indicate...

journal_title：Journal of clinical microbiology

authors： Shinoda T,Kaufman L,Padhye AA

更新日期：1981-03-01 00:00:00

abstract：:Haemophilus influenzae type b (Hib) is, in contrast to non-type b H. influenzae, associated with severe invasive disease, such as meningitis and epiglottitis, in small children. To date, accurate H. influenzae capsule typing requires PCR, a time-consuming and cumbersome method. Matrix-assisted laser desorption ionizat...

journal_title：Journal of clinical microbiology

authors： Månsson V,Resman F,Kostrzewa M,Nilson B,Riesbeck K

更新日期：2015-07-01 00:00:00

abstract：:We analyzed a collection of 97 well-characterized Burkholderia cepacia genomovar III isolates to evaluate multiple genomic typing systems, including pulsed-field gel electrophoresis (PFGE), BOX-PCR fingerprinting and random amplified polymorphic DNA (RAPD) typing. The typeability, reproducibility, and discriminatory p...

journal_title：Journal of clinical microbiology

authors： Coenye T,Spilker T,Martin A,LiPuma JJ

更新日期：2002-09-01 00:00:00

abstract：:PCR detecting the protein D (hpd) and fuculose kinase (fucK) genes showed high sensitivity and specificity for identifying Haemophilus influenzae and differentiating it from H. haemolyticus. Phylogenetic analysis using the 16S rRNA gene demonstrated two distinct groups for H. influenzae and H. haemolyticus. ...

journal_title：Journal of clinical microbiology

authors： Theodore MJ,Anderson RD,Wang X,Katz LS,Vuong JT,Bell ME,Juni BA,Lowther SA,Lynfield R,MacNeil JR,Mayer LW

更新日期：2012-04-01 00:00:00

abstract：:Group A streptococcus (GAS) species cause bacterial pharyngitis in both adults and children. Early and accurate diagnosis of GAS is important for appropriate antibiotic therapy to prevent GAS sequalae. The Revogene Strep A molecular assay (Meridian Bioscience Canada Inc, Quebec City, QC, Canada) is an automated real-t...

journal_title：Journal of clinical microbiology

authors： Banerjee D,Michael J,Schmitt B,Salimnia H,Mhaissen N,Goldfarb DM,Lachance P,Faron ML,Aufderheide T,Ledeboer N,Weissfeld A,Selvarangan R

更新日期：2020-06-24 00:00:00

abstract：:The causative agent of Johne's disease is Mycobacterium avium subsp. paratuberculosis. This is a chronic, debilitating gastrointestinal disorder that affects ruminants and is responsible for significant economic loss. The specimen processing method that combines C(18)-carboxypropylbetaine (CB-18) treatment and lytic e...

journal_title：Journal of clinical microbiology

authors： Thornton CG,MacLellan KM,Stabel JR,Carothers C,Whitlock RH,Passen S

更新日期：2002-05-01 00:00:00

abstract：:Five strains of an unknown, multidrug-resistant coryneform, gram-positive rod were isolated from blood, bronchial aspirate, and abscess specimens. Four of the five strains isolated were highly resistant to antimicrobial agents, including beta-lactams, aminoglycosides, macrolides, quinolones, and tetracyclines, except ...

journal_title：Journal of clinical microbiology

authors： Otsuka Y,Kawamura Y,Koyama T,Iihara H,Ohkusu K,Ezaki T

更新日期：2005-08-01 00:00:00

abstract：:Previous studies have reported the increased sensitivity of PCR targeting AF146527 over that of PCR targeting the B1 gene for diagnosis of toxoplasmosis. The present study suggests that the AF146527 element was absent in 4.8% of human Toxoplasma gondii-positive samples tested. The data argue that the B1 gene may be th...

journal_title：Journal of clinical microbiology

authors： Wahab T,Edvinsson B,Palm D,Lindh J

更新日期：2010-02-01 00:00:00

abstract：:In a clinical trial, Strep-A-Chek (a 10-min chromogenic test) was compared with the bacitracin disk susceptibility test for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes. Among 461 isolates of beta-hemolytic streptococci (344 throat isolates and 117 isolates from other sites)...

journal_title：Journal of clinical microbiology

authors： Yajko DM,Lawrence J,Nassos P,Young J,Hadley WK

更新日期：1986-09-01 00:00:00

abstract：:Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trich...

journal_title：Journal of clinical microbiology

authors： de Almeida JN Jr,Favero Gimenes VM,Francisco EC,Machado Siqueira LP,Gonçalves de Almeida RK,Guitard J,Hennequin C,Colombo AL,Benard G,Rossi F

更新日期：2017-08-01 00:00:00

abstract：:We compared the new Abbott TestPack (TP) respiratory syncytial virus (RSV) enzyme immunoassay (EIA) with cell culture and two commercial RSV EIAs (from Abbott Diagnostics and Kallestad Laboratories) by using split samples of fresh nasal washings from children with suspected RSV disease. Two tubes of HEp-2 cells were i...

journal_title：Journal of clinical microbiology

authors： Wren CG,Bate BJ,Masters HB,Lauer BA

更新日期：1990-06-01 00:00:00

abstract：:Between November 1983 and January 1984, 13 cases of Candida parapsilosis endophthalmitis occurred in Florida, Georgia, and Tennessee in patients who had had an intraocular lens implantation (IOLI) or cataract extraction with an IOLI. This outbreak followed the introduction in July 1983 of a new brand of balanced salt ...

journal_title：Journal of clinical microbiology

authors： McCray E,Rampell N,Solomon SL,Bond WW,Martone WJ,O'Day D

更新日期：1986-10-01 00:00:00

abstract：:Production of staphylococcal alpha- or alpha-beta-toxins correlated well with production of coagulase or thermonuclease (or both) in 203 Staphylococcus aureus isolates from milk and should be reliable indicators of S. aureus in the absence of Staphylococcus intermedius. Failures to produce toxin, tube coagulase, or th...

journal_title：Journal of clinical microbiology

authors： Jasper DE,Infante F,Dellinger JD

更新日期：1985-04-01 00:00:00

abstract：:The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The t...

journal_title：Journal of clinical microbiology

authors： Janda WM,Zigler KL,Bradna JJ

更新日期：1987-02-01 00:00:00

abstract：:We recently developed a molecular typing system for Campylobacter jejuni and Campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaA (I.Nachamkin, K. Bohachick, and C.M. Patton, J. Clin. Microbiol. 31:1531-1536, 1993). We extended the typing system to 83 flagellin types...

journal_title：Journal of clinical microbiology

authors： Nachamkin I,Ung H,Patton CM

更新日期：1996-02-01 00:00:00

abstract：:Twenty-nine Mycobacterium reference strains representing 10 species and 60 mycobacterial cultures isolated from sputum specimens were studied. These cultures were grown in Bactec 7H12B medium (Becton Dickinson and Co., Paramus, N.J.) supplemented with oleic acid-albumin-dextrose-catalase enrichment broth (Becton Dicki...

journal_title：Journal of clinical microbiology

authors： Chou S,Chedore P,Haddad A,Paul NR,Kasatiya S

更新日期：1996-05-01 00:00:00

abstract：:In titrating serum immunoglobulin G antibody to viruses by enzyme-linked immunosorbent assay, we used two rows of wells for serial twofold dilutions of the serum; in one row, a low concentration of a protein denaturant, 0.5 or 1.0 M guanidine hydrochloride, was added to the diluent so that the binding of low-avidity a...

journal_title：Journal of clinical microbiology

authors： Inouye S,Hasegawa A,Matsuno S,Katow S

更新日期：1984-09-01 00:00:00

abstract：:The binding of human immunoglobulin G by Fc receptors in herpes simplex virus (HSV)-infected cells can cause false-positive interpretations in the immunofluorescence test for HSV antibody. When the infected cell smears were treated with 10% glacial acetic acid for 5 min and rinsed in phosphate-buffered saline before t...

journal_title：Journal of clinical microbiology

authors： Gallo D

更新日期：1986-10-01 00:00:00

abstract：:The emergence of azole-resistant strains of Aspergillus fumigatus during treatment for aspergillosis occurs by a mutation selection process. Understanding how antifungal resistance mechanisms evolve in the host environment during infection is of great clinical importance and biological interest. Here, we used next-gen...

journal_title：Journal of clinical microbiology

authors： Hagiwara D,Takahashi H,Watanabe A,Takahashi-Nakaguchi A,Kawamoto S,Kamei K,Gonoi T

更新日期：2014-12-01 00:00:00

abstract：:In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%...

journal_title：Journal of clinical microbiology

authors： Amornchai P,Chierakul W,Wuthiekanun V,Mahakhunkijcharoen Y,Phetsouvanh R,Currie BJ,Newton PN,van Vinh Chau N,Wongratanacheewin S,Day NP,Peacock SJ

更新日期：2007-11-01 00:00:00

abstract：:Studies of human immunodeficiency virus type 1 (HIV-1) transmission suggest that genital HIV-1 RNA and DNA may both be determinants of HIV-1 infectivity. Despite its potential role in HIV-1 transmission, there are limited quantitative data on genital HIV-1 DNA. Here we validated an in-house real-time PCR method for qu...

journal_title：Journal of clinical microbiology

authors： Benki S,McClelland RS,Emery S,Baeten JM,Richardson BA,Lavreys L,Mandaliya K,Overbaugh J

更新日期：2006-12-01 00:00:00

abstract：:Total DNA from five Campylobacter species was digested with a mixture of XhoI and BglII restriction endonucleases and analyzed by Southern hybridization by using a probe complementary to the DNA coding for the 16S rRNA. Each of the Campylobacter species, including C. jejuni, C. coli, C. laridis, C. fetus, and C. upsal...

journal_title：Journal of clinical microbiology

authors： Moureau P,Derclaye I,Gregoire D,Janssen M,Cornelis GR

更新日期：1989-07-01 00:00:00

abstract：:Two closely related species of Rochalimaea, Rochalimaea quintana and Rochalimaea henselae, are nutritionally fastidious but can be cultivated on bacteriologic media from the blood of patients with diverse clinical presentations. We report a case of culture-proven R. henselae bacteremia in a child with persistent fever...

journal_title：Journal of clinical microbiology

authors： Welch DF,Hensel DM,Pickett DA,San Joaquin VH,Robinson A,Slater LN

更新日期：1993-09-01 00:00:00

abstract：:Accurate identification of pathogenic species is important for early appropriate patient management, but growing diversity of infectious species/strains makes the identification of clinical yeasts increasingly difficult. Among conventional methods that are commercially available, the API ID32C, AuxaColor, and Vitek 2 ...

journal_title：Journal of clinical microbiology

authors： Posteraro B,Efremov L,Leoncini E,Amore R,Posteraro P,Ricciardi W,Sanguinetti M

更新日期：2015-08-01 00:00:00

abstract：:Two hundred seventy nasopharyngeal aspirates were tested in duplicate with the Ortho Diagnostics, Inc. (Raritan, N.J.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. The test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. The enzyme-linked immunosorbent assay det...

journal_title：Journal of clinical microbiology

authors： Bromberg K,Tannis G,Daidone B,Clarke L,Sierra MF

更新日期：1985-12-01 00:00:00

abstract：:Recent evidence supports a causal relationship between Bartonella (Rochalimaea) henselae, cat-scratch disease (CSD), and bacillary angiomatosis. Cats appear to be the primary reservoir. Blood from 19 cats owned by 14 patients diagnosed with CSD was cultured. Blood samples from cats owned by veterinary students (n = 25...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Wilson KH,Sexton DJ,Hadfield TL,Berkhoff HA,Breitschwerdt EB

更新日期：1995-12-01 00:00:00