Comparative evaluation of the Iatron serological Candida check kit and the API 20C kit for identification of medically important Candida species.

abstract：:An enzyme-linked immunosorbent assay for the measurement of antibodies directed against cell surface antigens of Vibrio cholerae (CSA ELISA) was developed. NaN3-killed whole cells of V. cholerae, adsorbed to polystyrene tubes, were used as immobilized antigens. The assay was capable of detecting antibodies directed ag...

journal_title：Journal of clinical microbiology

authors： Cryz SJ Jr,Fürer E,Germanier R

更新日期：1982-07-01 00:00:00

abstract：:Helicobacter pylori cagA and vacA genotypes have been used for almost a decade as stable entities to link the severity of gastritis and ulcer disease. We describe here microevolution of the two genomic islands, cag pathogenicity island (cagPAI; 40 kb) and tfs3 (16 kb) from isolates obtained at inclusion (one subclone)...

journal_title：Journal of clinical microbiology

authors： Alvi A,Devi SM,Ahmed I,Hussain MA,Rizwan M,Lamouliatte H,Mégraud F,Ahmed N

更新日期：2007-12-01 00:00:00

abstract：:A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was...

journal_title：Journal of clinical microbiology

authors： Rench MA,Metzger TG,Baker CJ

更新日期：1984-11-01 00:00:00

abstract：:A new blood lysis-filtration culture technique revealed a high incidence of Staphylococcus epidermidis in the blood of patients and of healthy people. Of 2,004 blood cultures from patients, the blood lysis method grew S. epidermidis in 233 (11.6%), whereas a conventional two-bottle culture system grew this organism in...

journal_title：Journal of clinical microbiology

authors： Zierdt CH

更新日期：1983-04-01 00:00:00

abstract：:Eight genotypes (A to H) and nine subtypes (adw2, adw4, ayw1, ayw2, ayw3, ayw4, adrq+, adrq-, and ayr) of hepatitis B virus (HBV) have been identified worldwide. They appear to be associated with geographical distribution, virological characteristics, and possibly clinical outcomes. We performed sequence analysis of p...

journal_title：Journal of clinical microbiology

authors： Lusida MI,Nugrahaputra VE,Soetjipto,Handajani R,Nagano-Fujii M,Sasayama M,Utsumi T,Hotta H

更新日期：2008-07-01 00:00:00

abstract：:We evaluated three rapid identification systems-The Biomerieux rapid ID 32 STREP (ID32), the BBL Crystal rapid gram-positive identification (Crystal), and the Remel IDS RapID STR (IDS) systems-for their ability to identify 7 strains of Alloiococcus otitidis, 27 strains of Dolosigranulum pigrum, 3 strains of Ignavigran...

journal_title：Journal of clinical microbiology

authors： LaClaire LL,Facklam RR

更新日期：2000-06-01 00:00:00

abstract：:Maternal prenatal screening for group B streptococci (GBS) followed by offering of intrapartum chemoprophylaxis to carriers is one of the strategies used to reduce the incidence of neonatal early-onset GBS infections. Culturing of vaginal and anorectal swab specimens in selective broth is the screening procedure recom...

journal_title：Journal of clinical microbiology

authors： Hansen SM,Sørensen UB

更新日期：2003-04-01 00:00:00

abstract：:A commercially available endotoxin assay (CS-TAL) employing a chromogenic peptide and an amebocyte lysate from the Japanese horseshoe crab, Tachypleus tridentatus, gave a positive result with aqueous extracts of all 15 strains of Candida albicans and 1 strain each of Candida tropicalis, Cryptococcus neoformans, and a ...

journal_title：Journal of clinical microbiology

authors： Hodes DS,Heon D,Hass A,Hyatt AC,Hodes HL

更新日期：1987-09-01 00:00:00

abstract：:In 1997 and 1998, H3N2 influenza A viruses emerged among pigs in North America. Genetic analyses of the H3N2 isolates demonstrated that they had distinctly different genotypes. The most commonly isolated viruses in the United States have a triple-reassortant genotype, with the hemagglutinin, neuraminidase, and PB1 pol...

journal_title：Journal of clinical microbiology

authors： Landolt GA,Karasin AI,Phillips L,Olsen CW

更新日期：2003-05-01 00:00:00

abstract：:In this study, the optimal combination of three commercial glycoprotein G-2 (gG-2)-based herpes simplex virus type 2 (HSV-2) type-specific enzyme-linked immunosorbent assays (Euroimmun anti-HSV-2 immunoglobulin G [IgG] ELISA [Eu2], Gull HSV-2-specific IgG ELISA [Gu2], and Radim HSV-2 IgG ELISA [Ra2]) and one gG-2-base...

journal_title：Journal of clinical microbiology

authors： Eing BR,Lippelt L,Lorentzen EU,Hafezi W,Schlumberger W,Steinhagen K,Kühn JE

更新日期：2002-02-01 00:00:00

abstract：:Fifty-three isolates of Streptococcus uberis from various sources were examined for the presence of a common antigen. Initially, a serum was produced in rabbits which, by using rocket line immunoelectrophoresis, proved to react with identity to all of the S. uberis crude extracts as well as group B and E streptococcal...

journal_title：Journal of clinical microbiology

authors： Jones KF,Norcross NL

更新日期：1983-05-01 00:00:00

abstract：:Nineteen multidrug-resistant (MDR) Mycobacterium complex strains isolated in a nosocomial outbreak were characterized at the molecular level. The strains were microbiologically characterized as Mycobacterium bovis. The mpt40 sequence was not present in chromosomal DNA from these strains, supporting the fact that they ...

journal_title：Journal of clinical microbiology

authors： Blázquez J,Espinosa de Los Monteros LE,Samper S,Martín C,Guerrero A,Cobo J,Van Embden J,Baquero F,Gómez-Mampaso E

更新日期：1997-06-01 00:00:00

abstract：:The Leifson staining method was used to diagnose Helicobacter pylori infection and was compared to histology, culture, and the rapid urease test (RUT). Histology gave the best sensitivity (98%), compared to Leifson staining (97%), culture (92%), and RUT (85%) (P < 0.005). Leifson staining is a sensitive, rapid, econom...

journal_title：Journal of clinical microbiology

authors： Piccolomini R,Di Bonaventura G,Neri M,Di Girolamo A,Catamo G,Pizzigallo E

更新日期：1999-01-01 00:00:00

abstract：:Septic arthritis of the knee developed in a 21-month-old child. The causative organism, isolated from two separate arthrocenteses, was identified as Capnocytophaga ochracea morphologically and by biochemical reactions. Previous human infections (bacteremias) have occurred in granulocytopenic hosts with concomitant ora...

journal_title：Journal of clinical microbiology

authors： Winn RE,Chase WF,Lauderdale PW,McCleskey FK

更新日期：1984-04-01 00:00:00

abstract：:A group of 300 clinically derived isolates of coagulase-negative staphylococci were tested in parallel with the API STAPH-IDENT system (Analytab Products) and 14 conventional biochemical tests contained in Kloos and Schleifer's simplified scheme for identification of human Staphylococcus species. STAPH-IDENT is a mini...

journal_title：Journal of clinical microbiology

authors： Almeida RJ,Jorgensen JH

更新日期：1983-08-01 00:00:00

abstract：:We examined several factors for their effects on the serological reactivity of Legionella antigens used for direct or indirect fluorescent-antibody tests. These factors included media, methods of killing, strain differences, and the nature of the reactivity with diverse human sera. The maximum serological reactivities...

journal_title：Journal of clinical microbiology

authors： Benson RF,Malcolm GB,Pine L,Harrell WK

更新日期：1983-05-01 00:00:00

abstract：:A total of 272 staphylococcal isolates from cases of bovine mastitis (159 Staphylococcus aureus) belonging to 12 different species were identified with ID32 STAPH galleries, and 51 of them were confirmed by 16S rRNA gene (rrs) sequencing. The same isolates were examined for their hemolytic activity on sheep blood agar...

journal_title：Journal of clinical microbiology

authors： Boerlin P,Kuhnert P,Hüssy D,Schaellibaum M

更新日期：2003-02-01 00:00:00

abstract：:We examined mutations in the dihydropteroate synthase (DHPS) genes of Pneumocystis carinii f. sp. hominis (P. carinii) strains isolated from 24 patients with P. carinii pneumonia (PCP) in Japan. DHPS mutations were identified at amino acid positions 55 and/or 57 in isolates from 6 (25.0%) of 24 patients. The underlyin...

journal_title：Journal of clinical microbiology

authors： Takahashi T,Hosoya N,Endo T,Nakamura T,Sakashita H,Kimura K,Ohnishi K,Nakamura Y,Iwamoto A

更新日期：2000-09-01 00:00:00

abstract：:Amphotericin B therapy continues to be the "gold standard" in the treatment of invasive aspergillosis in the immunocompromised host. Although Aspergillus fumigatus and Aspergillus flavus constitute the major species, several reports have described invasive pulmonary or disseminated disease due to the less common Asper...

journal_title：Journal of clinical microbiology

authors： Sutton DA,Sanche SE,Revankar SG,Fothergill AW,Rinaldi MG

更新日期：1999-07-01 00:00:00

abstract：:A gag-based molecular beacon assay utilizing real-time nucleic acid sequence-based amplification technology has been developed to differentiate between the two genetic subclusters of human immunodeficiency virus type 1 (HIV-1) subtype C (C and C') circulating in Ethiopia. Of 41 samples, 36 could be classified as C or ...

journal_title：Journal of clinical microbiology

authors： Ayele W,Pollakis G,Abebe A,Fisseha B,Tegbaru B,Tesfaye G,Mengistu Y,Wolday D,van Gemen B,Goudsmit J,Dorigo-Zetsma W,de Baar MP

更新日期：2004-04-01 00:00:00

abstract：:We have developed and validated a nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma venereum (LGV). The 11 probes used in the NRT-PCR correctly identified the DNA from D to K and L...

journal_title：Journal of clinical microbiology

authors： Jalal H,Stephen H,Alexander S,Carne C,Sonnex C

更新日期：2007-08-01 00:00:00

abstract：:Seventy-nine methicillin-resistant Staphylococcus aureus (MRSA) strains, isolated during 1980 to 1990, were classified as MRSA Aggl- (14 strains) and MRSA Aggl+ (65 strains) strains on the basis of test results in slide agglutination assays designed to detect fibrinogen-binding protein (clumping factor) and protein A ...

journal_title：Journal of clinical microbiology

authors： Kuusela P,Hildén P,Savolainen K,Vuento M,Lyytikäinen O,Vuopio-Varkila J

更新日期：1994-01-01 00:00:00

abstract：:The API STAPH-IDENT system was compared with conventional methods for the identification of 14 Staphylococcus species. Conventional methods included the Kloos and Schleifer simplified scheme and DNA-DNA hybridization. The API STAPH-IDENT strip utilizes a battery of 10 miniaturized biochemical tests, including alkaline...

journal_title：Journal of clinical microbiology

authors： Kloos WE,Wolfshohl JF

更新日期：1982-09-01 00:00:00

abstract：:Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific prim...

journal_title：Journal of clinical microbiology

authors： Githui WA,Wilson SM,Drobniewski FA

更新日期：1999-04-01 00:00:00

abstract：:We recently developed a mass spectrometry (MS) procedure based on the detection of a serum disaccharide (MS-DS) in patients with invasive candidiasis (IC). Here, we compare the performance of MS-DS for the diagnosis of IC, invasive aspergillosis (IA), and mucormycosis (MM) with those of commercially available antigen ...

journal_title：Journal of clinical microbiology

authors： Mery A,Sendid B,François N,Cornu M,Poissy J,Guerardel Y,Poulain D

更新日期：2016-11-01 00:00:00

abstract：:There is a lack of consensus among investigators who use a variety of immunoassay techniques (e.g., enzyme-linked immunosorbent assay [ELISA] and radioimmunoassay) regarding the protocols for describing and forming standard reference or calibration curves and interpolating serum antibody concentrations. This confounds...

journal_title：Journal of clinical microbiology

authors： Plikaytis BD,Holder PF,Pais LB,Maslanka SE,Gheesling LL,Carlone GM

更新日期：1994-10-01 00:00:00

abstract：:Recent evidence supports a causal relationship between Bartonella (Rochalimaea) henselae, cat-scratch disease (CSD), and bacillary angiomatosis. Cats appear to be the primary reservoir. Blood from 19 cats owned by 14 patients diagnosed with CSD was cultured. Blood samples from cats owned by veterinary students (n = 25...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Wilson KH,Sexton DJ,Hadfield TL,Berkhoff HA,Breitschwerdt EB

更新日期：1995-12-01 00:00:00

abstract：:A simple method of storage and retrieval of serum samples for adenovirus serology is described. Complement-fixing antibodies remained stable for at least 5 years when stored at -10 degrees C or below. ...

journal_title：Journal of clinical microbiology

authors： Edwards EA

更新日期：1977-02-01 00:00:00

abstract：:The use of high-risk human papillomavirus (HPV) testing for surveillance and clinical applications is increasing globally, and it is important that tests are evaluated to ensure they are fit for this purpose. In this study, the performance of a new HPV genotyping test, the Papilloplex high-risk HPV (HR-HPV) test, was ...

journal_title：Journal of clinical microbiology

authors： Bhatia R,Serrano I,Wennington H,Graham C,Cubie H,Boland E,Fu G,Cuschieri K

更新日期：2018-02-22 00:00:00

abstract：:The data available for the diagnosis of chlamydial infections in infants which compare direct fluorescent-monoclonal-antibody stains (DFAs) with culture are limited to one reagent, MicroTrak (Syva Inc., Palo Alto, Calif.). We therefore performed a comparison of Pathfinder (Kallestad Diagnostics, Chaska, Minn.) and Mic...

journal_title：Journal of clinical microbiology

authors： Roblin PM,Hammerschlag MR,Cummings C,Williams TH,Worku M

更新日期：1989-05-01 00:00:00