Septic arthritis involving Capnocytophaga ochracea.

abstract：:We have evaluated the GenoType blood culture assay (Hain Lifescience, Nehren, Germany) for the identification of bacteria in 233 positive blood cultures and assessed its suitability in the workflow of a routine microbiology laboratory. In 68/233 (29.2%) samples, the culture result could not be confirmed by the GenoTyp...

journal_title：Journal of clinical microbiology

authors： Karumaa S,Kärpänoja P,Sarkkinen H

更新日期：2012-03-01 00:00:00

abstract：:During an outbreak of diarrheal illness among residents of a trailer park in rural Vermont, 37 (30%) of 122 residents met the case definition of outbreak-related giardiasis. Convalescent-phase sera from 24 residents and 20 nonresident control subjects were tested by enzyme-linked immunosorbent assay for immunoglobulin...

journal_title：Journal of clinical microbiology

authors： Birkhead G,Janoff EN,Vogt RL,Smith PD

更新日期：1989-08-01 00:00:00

abstract：:The findings from a preliminary assessment of a new instrument designed for the inoculation and spreading of specimens for microbiological analysis onto agar plates are described. The study found that the instrument was able to select full or biplates from a number of input cassettes, each containing different agar ty...

journal_title：Journal of clinical microbiology

authors： Glasson JH,Guthrie LH,Nielsen DJ,Bethell FA

更新日期：2008-04-01 00:00:00

abstract：:IS6110 restriction fragment length polymorphism (RFLP) genotyping is the most widely used genotyping method to study the epidemiology of Mycobacterium tuberculosis. However, due to the complexity of the IS6110 RFLP genotyping technique, and the interpretation of RFLP data, mycobacterial interspersed repetitive-unit-va...

journal_title：Journal of clinical microbiology

authors： Hanekom M,van der Spuy GD,Gey van Pittius NC,McEvoy CR,Hoek KG,Ndabambi SL,Jordaan AM,Victor TC,van Helden PD,Warren RM

更新日期：2008-10-01 00:00:00

abstract：:The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tåstrup, Denmark) and the Staph ID 32 API system (API System, BioMérieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated....

journal_title：Journal of clinical microbiology

authors： Renneberg J,Rieneck K,Gutschik E

更新日期：1995-05-01 00:00:00

abstract：:Cefaclor is less stable than most cephalosporins in media at 35 degrees C. We demonstrated that the activity of cefaclor in Mueller-Hinton agar diminishes continuously at 4 degrees C, resulting in a loss of two-thirds of the activity within 21 days. We recommend that agar dilution plates for this cephalosporin be prep...

journal_title：Journal of clinical microbiology

authors： Surprenant AM,Preston DA

更新日期：1985-01-01 00:00:00

abstract：:Microagglutination and micro-enzyme-linked immunosorbent assay (ELISA) tests with easily prepared, safe, heat-killed antigens for detecting antibodies to the legionnaires disease organism have been developed. A safranin-stained whole-cell antigen is used in the microagglutination test, and a simply prepared soluble an...

journal_title：Journal of clinical microbiology

authors： Farshy CE,Klein GC,Feeley JC

更新日期：1978-04-01 00:00:00

abstract：:A 55-year-old man with severe peripheral vascular disease developed nosocomial septicemia which was caused by the gram-negative bacterium CDC group IV c-2, presumably from a plantar abscess on the left foot. Recovery followed amputation of the infected extremity and antibiotic therapy. This is the first reported case ...

journal_title：Journal of clinical microbiology

authors： Crowe HM,Brecher SM

更新日期：1987-11-01 00:00:00

abstract：:Extended-spectrum-beta-lactamase (ESBL)-producing members of the Enterobacteriaceae are often resistant to multiple drug classes, making therapy of urinary infections with oral antibiotics difficult. Previously it was shown that amoxicillin-clavulanate can provide clavulanate inhibition of ESBLs and protect an oral ce...

journal_title：Journal of clinical microbiology

authors： Campbell JD,Lewis JS 2nd,McElmeel ML,Fulcher LC,Jorgensen JH

更新日期：2012-03-01 00:00:00

abstract：:Staphylococcus epidermidis, a human commensal, is a common cause of bacteremia in immunocompromised patients with indwelling medical devices. We report a case of isolated cervical adenitis caused by S. epidermidis in an immunocompetent patient and comment on the presumed pathogenesis. ...

journal_title：Journal of clinical microbiology

authors： Ryan-Poirier K,Patrick CC

更新日期：1993-02-01 00:00:00

abstract：:The 37-kDa protein (P37) of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The P37 gene was cloned from a B. burgdorferi genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to ...

journal_title：Journal of clinical microbiology

authors： Gilmore RD Jr,Murphree RL,James AM,Sullivan SA,Johnson BJ

更新日期：1999-03-01 00:00:00

abstract：:A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. Th...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Tsunomori Y,Seki R

更新日期：2003-11-01 00:00:00

abstract：:The hemagglutination inhibition test (HAI) and the enzyme-linked immunosorbent assay (ELISA) for detecting antibody to rubella virus were compared by testing 25 sets of paired sera taken before and after infection and 10 sets of sera taken during acute and convalescent stages of the disease and by screening 700 serum ...

journal_title：Journal of clinical microbiology

authors： Shekarchi IC,Sever JL,Tzan N,Ley A,Ward LC,Madden D

更新日期：1981-05-01 00:00:00

abstract：:The performance of four media for the isolation of Aeromonas strains from stool specimens, the importance of ampicillin-susceptible Aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for Aeromonas strains were evaluated in two phases. In the fi...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Stroh EM,Jessop J

更新日期：1988-09-01 00:00:00

abstract：:In Asia, bla(KPC) detection has been limited to East Asia and not yet seen in Southeast Asia. We report four bla(KPC-2)-containing Klebsiella pneumoniae isolates from two different hospitals in Singapore. All isolates belonged to strain type 11 (ST11) and were indistinguishable by pulsed-field gel electrophoresis (PFG...

journal_title：Journal of clinical microbiology

authors： Balm MN,Ngan G,Jureen R,Lin RT,Teo J

更新日期：2012-02-01 00:00:00

abstract：:The virulence plasmid of Escherichia coli O157 strain EDL933 carries a 10-kb putative virulence gene designated toxB. Little is known about the distribution of this gene among E. coli O157 strains or its presence in other enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) strains. We developed PCR an...

journal_title：Journal of clinical microbiology

authors： Tozzoli R,Caprioli A,Morabito S

更新日期：2005-08-01 00:00:00

abstract：:Data pertaining to 20 strains of Yensinia enterocolitica isolated from the cecal content of swine slaughtered at Toronto Abattoirs are presented. Fifteen of 20 cultures belonged to the two predominant human serotypes in Ontario (i.e., serotype O:3 and O:5,27.) Seven cultures of Y. enterocolitica O:3 belonged to the "C...

journal_title：Journal of clinical microbiology

authors： Toma S,Deidrick VR

更新日期：1975-12-01 00:00:00

abstract：:Viral hemorrhagic fevers, because of their high mortality rates, the lack of medical countermeasures, and their potential use as instruments of bioterrorism, pose a significant threat to the developed and the developing areas of the world. The key to preventing the spread of these diseases is early and accurate detect...

journal_title：Journal of clinical microbiology

authors： Satterly NG,Voorhees MA,Ames AD,Schoepp RJ

更新日期：2016-12-28 00:00:00

abstract：:The diagnostic gains of repeat testing for Clostridium difficile by enzyme immunoassay and PCR (i.e., initial negative result followed by positive result) within a 7-day period were 1.9 and 1.7%, respectively. There is little value of repeat testing for C. difficile by enzyme immunoassay or PCR. ...

journal_title：Journal of clinical microbiology

authors： Aichinger E,Schleck CD,Harmsen WS,Nyre LM,Patel R

更新日期：2008-11-01 00:00:00

abstract：:Developing interpretive breakpoints for any given organism-drug combination requires integration of the MIC distribution, pharmacokinetic and pharmacodynamic parameters, and the relationship between the in vitro activity and outcome from both in vivo and clinical studies. Using data generated by standardized broth mic...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Diekema DJ,Rex JH,Espinel-Ingroff A,Johnson EM,Andes D,Chaturvedi V,Ghannoum MA,Odds FC,Rinaldi MG,Sheehan DJ,Troke P,Walsh TJ,Warnock DW

更新日期：2006-03-01 00:00:00

abstract：:Mycobacteria include a large number of pathogens. Identification to species level is important for diagnoses and treatments. Here, we report the development of a Web-accessible database of the hsp65 locus sequences (http://msis.mycobacteria.info) from 149 out of 150 Mycobacterium species/subspecies. This database can ...

journal_title：Journal of clinical microbiology

authors： Dai J,Chen Y,Lauzardo M

更新日期：2011-06-01 00:00:00

abstract：:The pathogenesis of human rhinovirus (HRV) during severe respiratory disease remains undefined; thus, we aimed to explore the relationship between the HRV molecular subtyping results obtained during severe and asymptomatic childhood infections. Nasopharyngeal/oropharyngeal swabs from children (1 to 59 months of age) h...

journal_title：Journal of clinical microbiology

authors： Baillie VL,Moore DP,Mathunjwa A,Morailane P,Simões EAF,Madhi SA

更新日期：2019-08-26 00:00:00

abstract：:A blind volunteer was inoculated in one eye with an isolate of Chlamydia trachomatis in 1961 and followed for 20 years. During this time, many observations were made of his clinical responses to the first inoculation and several subsequent inoculations with the same and other strains, chlamydial shedding, and antibody...

journal_title：Journal of clinical microbiology

authors： Hanna L,Jawetz E,Dawson CR,Thygeson P

更新日期：1982-11-01 00:00:00

abstract：:The capsular-like envelope of Legionella pneumophila strains Togus 1 (serotype 2) and Philadelphia 1 (serotype 1) was isolated and purified by column chromatography on Sepharose 6B. Antibody raised in rabbits to these two antigenic materials did not cross-react in gel diffusion. Upon electrophoresis followed by gel di...

journal_title：Journal of clinical microbiology

authors： Smith RA,DiGiorgio S,Darner J,Wilhelm A

更新日期：1981-04-01 00:00:00

abstract：:A 2-h nonisotopic DNA probe assay for the direct detection of Neisseria gonorrhoeae in urogenital specimens has recently been modified (PACE 2; Gen-Probe, San Diego, Calif.). The new assay format was developed to increase the sensitivity of the assay and simplify procedural steps. In this study, the new DNA probe test...

journal_title：Journal of clinical microbiology

authors： Panke ES,Yang LI,Leist PA,Magevney P,Fry RJ,Lee RF

更新日期：1991-05-01 00:00:00

abstract：:A 4-h Micro-ID technique for direct identification of oxidase-negative gram-negative rods from positive blood cultures was compared to subculture and species identification of single colonies by API 20E and Micro-ID, using standardized inocula. A total of 127 patients (220 positive cultures) were studied. Isolates inc...

journal_title：Journal of clinical microbiology

authors： Appelbaum PC,Schick SF,Kellogg JA

更新日期：1980-10-01 00:00:00

abstract：:Fully standardized reproducible and sensitive quantification assays for cytomegalovirus (CMV) are needed to better define thresholds for antiviral therapy initiation and interruption. We evaluated the newly released Abbott RealTime CMV assay for CMV quantification in whole blood (WB) that includes automated extraction...

journal_title：Journal of clinical microbiology

authors： Schnepf N,Scieux C,Resche-Riggon M,Feghoul L,Xhaard A,Gallien S,Molina JM,Socié G,Viglietti D,Simon F,Mazeron MC,Legoff J

更新日期：2013-07-01 00:00:00

abstract：:The microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16S rRNA genes were randomly amplified by means of conserved eubacterial primers and cloned. Restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16S rRNA from the cult...

journal_title：Journal of clinical microbiology

authors： Dymock D,Weightman AJ,Scully C,Wade WG

更新日期：1996-03-01 00:00:00

abstract：:Lactococcus garvieae is a Gram-positive coccus that has morphological and biochemical similarities to enterococci. L. garvieae strains rare human pathogens, with only a few cases reported in the literature, mainly as a cause of infective endocarditis. L. garvieae is well known as a fish pathogen, and in some of the re...

journal_title：Journal of clinical microbiology

authors： Navas ME,Hall G,El Bejjani D

更新日期：2013-06-01 00:00:00

abstract：:A clustered regularly interspaced short palindromic repeat (CRISPR) typing method has recently been developed and used for typing and subtyping of Salmonella spp., but it is complicated and labor intensive because it has to analyze all spacers in two CRISPR loci. Here, we developed a more convenient and efficient meth...

journal_title：Journal of clinical microbiology

authors： Li H,Li P,Xie J,Yi S,Yang C,Wang J,Sun J,Liu N,Wang X,Wu Z,Wang L,Hao R,Wang Y,Jia L,Li K,Qiu S,Song H

更新日期：2014-08-01 00:00:00